• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1986.12a
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• pp.528.1-528
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• 1986

Cellulose utilising hybrids between Cellulomonas fimi and Bacillus pumilus were isolated after PEG mediated protoplast fusion. 33% (w/v) PEG #6, 000 and 50mM $Ca^{++}$were optimum concentration. The intergeneric fusion frequency was 3.2$\times$10$^{-7}$ xtracellular CMCase and $\beta$-glucosidase activities were detected from one hydrid unlike only CMCase was detected from Cellulomonas fimi.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.7 no.3
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• pp.539-557
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• 2013

A novel multifocus image fusion algorithm based on NSCT is proposed in this paper. In order to not only attain the image focusing properties and more visual information in the fused image, but also sensitive to the human visual perception, a local multidirection variance (LEOV) fusion rule is proposed for lowpass subband coefficient. In order to introduce more visual saliency, a modified local contrast is defined. In addition, according to the feature of distribution of highpass subband coefficients, a direction vector is proposed to constrain the modified local contrast and construct the new fusion rule for highpass subband coefficients selection The NSCT is a flexible multiscale, multidirection, and shift-invariant tool for image decomposition, which can be implemented via the atrous algorithm. The proposed fusion algorithm based on NSCT not only can prevent artifacts and erroneous from introducing into the fused image, but also can eliminate 'block effect' and 'frequency aliasing' phenomenon. Experimental results show that the proposed method achieved better fusion results than wavelet-based and CT-based fusion method in contrast and clarity.

• Korean Journal of Microbiology
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• v.22 no.1
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• pp.35-40
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• 1984

Attempts were made to optimise protoplast formation and regeneration methods to improve the protoplast fusion frequencies of Streptomyces coelicolor. The yields of protoplast formation and regeneration were varied with different growth phase of the culture. Maximum yields were obtained when cells were taken from the late logarithmic phase. Protoplast formation reached almost its maximum with lysozyme treatment at a concentration of 2mg/ml without any other lytic enzyme. A high frequency of protoplast regeneration was accomplished by overlay method: the method gave 14% recovery of regenerated protoplast versus 1.8% recovery for monolay method. A recombinant frequency of 1.8X10^-2 was obtained by protoplast fusion using PEG 1000(50% w/v).

• Reproductive and Developmental Biology
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• v.34 no.2
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• pp.73-79
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• 2010

We investigated the microtubule dynamics, including the inheritance of donor centrosomes and the mitotic spindle assembly occurring during the first mitosis of somatic cell nuclear transfer (SCNT) embryos in pigs. SCNT embryos were fixed 15 min and 1 h after fusion in order to assess the inheritance pattern of the donor centrosome. The distribution and dynamic of the centrosome and microtubule during the first mitotic phase of SCNT embryos were also evaluated. The frequency of embryos evidencing $\gamma$-tubulin spots (centrosome) was 93.2% in the SCNT embryos 15 min after fusion. In the majority of the SCNT embryos (61.5%), however, no centrosome was observed 1 h after fusion. The frequency of the embryos with no or abnormal mitotic spindles 20 h after fusion was 19.6%. The $\gamma$-tubulin spots were detected near the nuclei of somatic cells regardless of cell cycle phase, whereas $\gamma$-tubulin spots in the SCNT embryos were observed only during the inter-anaphase transition. These results showed that the donor centrosome is inherited into the SCNT embryos, but failed to assemble the normal mitotic spindles during first mitotic phase in some SCNT embryos.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.7
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• pp.3620-3637
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• 2019

Since the birth of Synthetic Aperture Radar (SAR), it has been widely used in the military field and so on. However, the existence of speckle noise makes a good deal inconvenience for the subsequent image processing. The continuous development of sparse representation (SR) opens a new field for the speckle suppressing of SAR image. Although the SR de-noising may be effective, the over-smooth phenomenon still has bad influence on the integrity of the image information. In this paper, one novel SAR image de-noising method based on residual image fusion and sparse representation is proposed. Firstly we can get the similar block groups by the non-local similar block matching method (NLS-BM). Then SR de-noising based on the adaptive K-means singular value decomposition (K-SVD) is adopted to obtain the initial de-noised image and residual image. The residual image is processed by Shearlet transform (ST), and the corresponding de-noising methods are applied on it. Finally, in ST domain the low-frequency and high-frequency components of the initial de-noised and residual image are fused respectively by relevant fusion rules. The final de-noised image can be recovered by inverse ST. Experimental results show the proposed method can not only suppress the speckle effectively, but also save more details and other useful information of the original SAR image, which could provide more authentic and credible records for the follow-up image processing.

• Smart Structures and Systems
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• v.6 no.9
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• pp.1025-1039
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• 2010

Measuring displacement response for civil structures is very important for assessing their performance, safety and integrity. Recently, video-based techniques that utilize low-cost high-resolution digital cameras have been developed for such an application. These techniques however have relatively low sampling frequency and the results are usually contaminated with noises. In this study, an integrated visual-inertial measurement method that combines a monocular videogrammetric displacement measurement technique and a collocated accelerometer is proposed for displacement and velocity measurement of civil engineering structures. The monocular videogrammetric technique extracts three-dimensional translation and rotation of a planar target from an image sequence recorded by one camera. The obtained displacement is then fused with acceleration measured from a collocated accelerometer using a multi-rate Kalman filter with smoothing technique. This data fusion not only can improve the accuracy and the frequency bandwidth of displacement measurement but also provide estimate for velocity. The proposed measurement technique is illustrated by a shake table test and a pedestrian bridge test. Results show that the fusion of displacement and acceleration can mitigate their respective limitations and produce more accurate displacement and velocity responses with a broader frequency bandwidth.

• Korean Journal of Microbiology
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• v.26 no.2
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• pp.82-87
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• 1988

The genetic analysis and characterization of protoplast fusion hybrids between complementary auxotrophic mutants of Candida pseudotropicalis were carried out. Nuclear fusion appeared to occur in fusion hybrids (e.g., F15 and F33), as strongly suggested by isolation of recombinants after mitotic segregation of parental genetic markers. This was confirmed by KNA content, nuclear staining and comparison of survival rate to UV light. After keeping fusion hybrids for approximately one year, the frequency of spontaneous mitotic segregation was $3.0\times 10^{-4}$ - $8.1\times 10^{-4}$ while that of induced mitotic segregation was $1.4\times 10^{-3}$- $1.7\times 10^{-3}$. These results suggested that they maintained stable karyogamy state. It was also found that the production of $\beta$-D-galactosidase from F15, F33 and F158 was somewhat increased when compared with that from either auxotrophic parents or wild type.

• Journal of Information Processing Systems
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• v.14 no.5
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• pp.1293-1300
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• 2018

For the disadvantages of multi-scale geometric analysis methods such as loss of definition and complex selection of rules in image fusion, an improved multi-focus image fusion method is proposed. First, the initial fused image is quickly obtained based on the lifting stationary wavelet transform, and a simple normalized cut is performed on the initial fused image to obtain different segmented regions. Then, the original image is subjected to NSCT transformation and the absolute value of the high frequency component coefficient in each segmented region is calculated. At last, the region with the largest absolute value is selected as the postfusion region, and the fused multi-focus image is obtained by traversing each segment region. Numerical experiments show that the proposed algorithm can not only simplify the selection of fusion rules, but also overcome loss of definition and has validity.

• Applied Microscopy
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• v.13 no.1
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• pp.31-40
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• 1983

Ultrathin sections of intact mycelia, released protoplast and fused protoplast of Micromonospora rosaria were observed by electron microscopy Intact mycelia showed a typical gram-positive bacterial cell wall structure and mesosomes. Released protoplasts had no cell wall components and fibrous nuclear region was distinguished from cytoplsmic region clearly. Protoplasts which treated with sucrose supplemented buffer were stable. But those treated with buffer without sucrose were extensively damaged, forming mom braneous vesicles. It was surmised that those vesicles originated from the damaged cytoplasmic membrane. High frequency of fusion was achieved by 50%(w/v). polyethylene-glycol 1,000 Fusion bodies in different stage of fusion were observed. Cell membrane barrier was stepwise relieved.

• Smart Structures and Systems
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• v.12 no.3_4
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• pp.271-290
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• 2013

The acceleration information is significant for the structural health monitoring, which is the basic measurement to identify structural dynamic characteristics and structural vibration. The efficiency of the accelerometer is subsequently important for the structural health monitoring. In this paper, the distance measure matrix and the support level matrix are constructed firstly and the synthesized support level and the fusion method are given subsequently. Furthermore, the synthesized support level can be served as the determination for diagnosis on accelerometers, while the consensus data fusion method can be used to recover the acceleration information in frequency domain. The acceleration acquisition measurements from the accelerometers located on the real structure National Aquatics Center are used to be the basic simulation data here. By calculating two groups of accelerometers, the validation and stability of diagnosis and recovering on acceleration based on the data fusion are proofed in the paper.