Proceedings of the Korean Society for Applied Microbiology Conference (한국미생물생명공학회:학술대회논문집)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Intergeneric protoplast fusion between Bacillus pumilus and Cellulomonas fimi

  • Kim, D.M. (Dept. Food Sci. & Technol., College of Agri Seoul National Univ.) ;
  • Lee, K.H. (Dept. Food Sci. & Technol., College of Agri Seoul National Univ.)
  • Published : 1986.12.01
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Abstract

Cellulose utilising hybrids between Cellulomonas fimi and Bacillus pumilus were isolated after PEG mediated protoplast fusion. 33% (w/v) PEG #6, 000 and 50mM $Ca^{++}$were optimum concentration. The intergeneric fusion frequency was 3.2$\times$10$^{-7}$ xtracellular CMCase and $\beta$-glucosidase activities were detected from one hydrid unlike only CMCase was detected from Cellulomonas fimi.

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