• Journal of Plant Biotechnology
• /
• v.1 no.1
• /
• pp.46-55
• /
• 1999

The expressed sequence tags(ESTs) from immature seed of rice, Oryza sativa cv Milyang 23, were partially sequenced and analyzed by homology. As of 1998, the partial sequences of about 6,600 cDNA clones were analyzed from normal and normalized immature seed cDNA libraries. About 2,200 ESTs were putatively identified by BLASTX deduced amino acid sequence homology analysis. About 20% of them were putatively identified as storage proteins. Also the clones were highly homologous to genes involved particularly in starch biosynthesis, glycolysis, signal transduction and defenses. Compared to 35% of redundancy in the ESTs of normal cDNA library, that from the substracted library was 15%. The Korea Rice Genome Network is maintained to provide the updated information of sequences, their homologies and sequence alignments of ESTs. For the stable expression of transgene in rice, diverse vectors were developed for overexpression, targeting and gene dosage effect with transit peptides (Tp) and matrix attachment region (MAR) sequence from chicken lysozyme locus. The rice calli were transformed via Agrobacterium tumefaciens LBA4404(pSB1) with the triparental mating technique and selected by herbicide resistance. The green fluorescent protein(GFP) gene in expression vector under the control of rbcS promoter-Tp was overexpressed upto 10 % of the total soluble protein. In addition, the Tp-sGFP fusion protein was properly processed during translocation into chloroplast. The expression of sGFP in the presence of MAR sequences was analyzed with Northern and immunoblot analysis. All the lines in which sGFP transgene with MAR sequence, showed position independent and copy number-dependent expression, while the lines without MAR showed the varied level of expression with the integration site. Thus the MAR sequence significantly reduced the variation in transgene expression between independent transformants.

• Archives of Plastic Surgery
• /
• v.41 no.1
• /
• pp.45-49
• /
• 2014

Background Preauricular sinuses are congenital abnormalities caused by a failure of fusion of the primitive tubercles from which the pinna is formed. When persistent or recurring inflammation occurs, surgical excision of the infected tissue should be considered. Preauricular defects inevitably occur as a result of excisions and are often difficult to resolve with a simple suture; a more effective reconstruction technique is required for treating these defects. Methods After total excision of a preauricular sinus, the defect was closed by a plastic surgeon. Based on the depth of the defect and the degree of tension when apposing the wound margins, the surgeon determined whether to use primary closure or a posterior auricular flap. Results A total of 28 cases were examined. In 5 cases, including 2 reoperations for dehiscence after primary repair, reconstruction was performed using posterior auricular transposition flaps. In 16 cases of primary closure, the defects were closed using simple sutures, and in 7 cases, closure was performed after wide undermining. Conclusions If a preauricular defect is limited to the subcutaneous layer and the margins can be easily approximated, primary closure by only simple suturing may be used to perform the repair. If the defect is deep enough to expose the perichondrium or if there is tension when apposing the wound margins, wide undermining should be performed before primary closure. If the extent of the excision exposes cartilage, the procedure follows dehiscence of the primary repair, or the tissue is not sufficiently healthy, the surgeon should use a posterior auricular flap.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.12 no.5
• /
• pp.261-265
• /
• 2012

The applications of augmented reality technology is increasing in the several field by IT convergence and we find augmented reality application easily in the smart device application area. And then, to attempt to use augmented reality technology in advantage, exhibitions and performances was activated. However, it less than other fields of augmented reality technology expertise yet. Among them services to Use in fusion with monitoring are lacking. In this paper, we proposed the augmented reality monitoring technology based on smart device. It is able to incorporate QR-code which is already building for the purpose of advertising promotional materials with augmented reality technology in many areas. Therefore, it is able to utilize unattended building or automation equipment facilities using QR-code and environmental monitoring sensor in the industry.

• Journal of the Korean Society of Costume
• /
• v.62 no.6
• /
• pp.165-181
• /
• 2012

Alexander McQueen is a typical designer of unconventional and experimental design who showed maximalism through outstanding tailoring. The purpose of this research is to study formative characteristics of maximalism that are expressed in modern fashion based on the works of Alexander McQueen, and to suggest boundless possibilities of development through his sense of design and the expressive ability of his works. Theoretical considerations will be made through literature research and precedent studies, and the collection data for case analysis will be used to select ready-to-wear women's collections from 2001 S/S to 2010 F/W by referring to internet sites. The study results are as follows. First, the most representative characteristic of formative characteristics of maximalism expressed in Alexander McQueen's works is expandability. Second, decorations used as a part of detail on clothes is used as decoration itself, and handcraft expression is shown through excessive use of decorations. The expandability of decoration that emphasizes splendid magnificence is expressed through flawless technique. Third, the fusion of each different image and the combination of ethnic image and modern image was most prevalent. Fourth, fantasy expresses a fictive image that could not be seen in reality by expressing an unrealistic and maximized image through the imagination of designer. Fifth, experimental element is expressed as an image that actively reflects the challenging spirit, which suggests a new direction of beauty through shocking and extraordinary images of the inner world of the designer and the root of new ideas about inhumane modern society.

• Journal of Korea Multimedia Society
• /
• v.16 no.9
• /
• pp.1031-1043
• /
• 2013

Extracting hand region from images is the first part in the process to recognize hand posture and gesture interaction. Therefore, a good segmenting method is important because it determines the overall performance of hand recognition systems. Conventional hand segmentation researches were prone to changing illumination conditions or limited to the ability to detect multiple people. In this paper, we propose a robust technique based on the fusion of skin-color data and depth information for hand segmentation process. The proposed algorithm uses skin-color data to localize accurate seed location for region-growing from a complicated background. Based on the seed location, our algorithm adjusts each detected blob to fill up the hole region. A region-growing algorithm is applied to the adjusted blob boundary at the detected depth image to obtain a robust hand region against illumination effects. Also, the resulting hand region is used to train our skin-model adaptively which further reduces the effects of changing illumination. We conducted experiments to compare our results with conventional techniques which validates the robustness of the proposed algorithm and in addition we show our method works well even in a counter light condition.

• Journal of Korean Neurosurgical Society
• /
• v.63 no.6
• /
• pp.777-783
• /
• 2020

Objective : To compare the accuracy and breach rates of freehand (FH) versus navigated (NV) pedicle screws in the thoracic and lumbar spine in patients with metastatic spinal tumors. Methods : A retrospective review of adult patients who underwent pedicle screw fixation in the thoracic or lumbar spine for metastatic spinal tumors between 2012 and 2018 was conducted. Breaches were assessed based on the Gertzbein and Robbins classification and only screws placed >4 mm outside of the pedicle wall (lateral or medial) were considered breached. Results : A total of 62 patients received 547 pedicle screws (average 8 per patient) - 34 patients received 298 pedicle screws in the FH group and 28 patients received 249 screws in the NV group. There were 40/547 breaches, corresponding to a breach and accuracy rate of 7.3% and 92.7%, respectively. The breach rate was 9.7% in the FH group and 4.4% in the NV group (chi-squared test, p=0.017); this corresponded to an accuracy rate of 90.3% and 95.6%, respectively. Only one patient from the overall cohort (in the FH group) required revision surgery due to a medial breach abutting the spinal cord (1.6% of all patients; 2.9% of FH patients); no patient suffered organ, vessel, or neurological injury from screw breaches. Conclusion : Navigated pedicle screw placement in patients with metastatic spinal tumors has a significantly higher radiographic accuracy compared to the FH technique. However, the revision surgery was low and no patient suffered from clinically-relevant breach. Navigation also offers the advantage of real-time localization of spinal tumors and aids in targeting and resection of these lesions.

• Journal of the Korean Society of Costume
• /
• v.52 no.3
• /
• pp.171-187
• /
• 2002

The purpose of this study is to grasp the meanings of retro-fashion, to research the design characteristics and background on the birth of repro-fashion. It can help to confirm the importance of aesthetic marketing which is based on human feelings, the roles and cultural and industrial meanings. Reto-fashion Is one of the mediums between the expression of Post-modern fashion and emotional sympath of human beings as consumer and fashion creators. So this inspiration is the way of fashion creativities. The backgrounds of appearance repro-fashion are reflection about materialism and technique and 20th century, the sense of instability and finding new ideas from the past. The characteristics of retro-fashion designs are as follows : First, the styles have been come from 50's. 60's, 70's, and 80\`s. It means that the styles not just expressed to return to the past simply but an expression the emotional state for missing the past. Second, the colors and materials are various also as like styles. Third, the decorations of repro-fashion are more crafts by human. The roles of retro-fashion are for creation of high valued product in fashion design, expression of individuality with disharmonized coordination and application as a fusion style. The cultural and industrial meaning of repro-fashion are endowment of aesthetic marketing using human feeling in fashion marketing area and pursuing of the Renaissance of fashion culture and industry. Therefore it is needed that design critics and analysis going side by culture and industry condition for fashion study with human feelings in 21th century.

• Journal of Information Processing Systems
• /
• v.14 no.5
• /
• pp.1176-1194
• /
• 2018

The object classification using the images' contents is a big challenge in computer vision. The superpixels' information can be used to detect and classify objects in an image based on locations. In this paper, we proposed a methodology to detect and classify the image's pixels' locations using enhanced bag of words (BOW). It calculates the initial positions of each segment of an image using superpixels and then ranks it according to the region score. Further, this information is used to extract local and global features using a hybrid approach of Scale Invariant Feature Transform (SIFT) and GIST, respectively. To enhance the classification accuracy, the feature fusion technique is applied to combine local and global features vectors through weight parameter. The support vector machine classifier is a supervised algorithm is used for classification in order to analyze the proposed methodology. The Pascal Visual Object Classes Challenge 2007 (VOC2007) dataset is used in the experiment to test the results. The proposed approach gave the results in high-quality class for independent objects' locations with a mean average best overlap (MABO) of 0.833 at 1,500 locations resulting in a better detection rate. The results are compared with previous approaches and it is proved that it gave the better classification results for the non-rigid classes.

• Korean Journal of Animal Reproduction
• /
• v.26 no.4
• /
• pp.329-338
• /
• 2002

This study was carried out to investigate the developmental rates of embryo reconstructed with different cell type and to estimate correlation of transcriptional level of octamer-binding transcription factor 4 (Oct4) and fibroblast growth factor 4 (FCF4) gene on peri-implantation stage embryos. Donor cells were transferred into perivitelline space of enucleated oocytes. The karyoplast-cytoplast couplets were accom- plished by cell to cell fusion and activated with ionomycin and 6-dimethylaminopurine. Reconstructed embryos were co-cultured with bovine oviduct epithelial cells in CR 1 aa medium. There is no difference in blastocyst formation rate following nuclear transfer UT) with fetal fibroblast cell (16/50; 32.0%), cumulus cell (16/49; 32.6%) and ear cell (17/52; 32.6%). The expression level of Oct4 and FCF4 in peri-implantation bovine embryo derived from in vitro fertilization (IVF) and NT were determined by reverse-transcription polymerase chain reaction (RT-PCR) technique. In peri-implantation of IVF result in a transient increased of FCF4 paralleled by an increased expression of Oct4. However, Oct4 gene was highly expressed in hatching blastocysts derived from NT compared to IVF. Also, FGF4 expression level in hatching blastocysts and outgrowth stage derived from NT was lower than that of IVF. In conclusion, it is suggested that the different transcription patterns observed in nuclear transfer embryos may lead to a lower rate of embryo development, implantation and pregnancy.

• Journal of the Korean Society of Manufacturing Technology Engineers
• /
• v.24 no.6
• /
• pp.703-709
• /
• 2015

In this work, a robot aimed at grapping and delivering an object by using a simple finger-pointing command from a hand- or arm-handicapped person is introduced. In this robot system, a Leap Motion sensor is utilized to obtain the finger-motion data of the user. In addition, a Kinect sensor is also used to measure the 3D (Three Dimensional)-position information of the desired object. Once the object is pointed at through the finger pointing of the handicapped user, the exact 3D information of the object is determined using an image processing technique and a coordinate transformation between the Leap Motion and Kinect sensors. It was found that the information obtained is transmitted to the robot controller, and that the robot eventually grabs the target and delivers it to the handicapped person successfully.