• 한국버섯학회지
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• 제2권3호
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• pp.157-162
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• 2004

이 실험의 목적은 침엽수(Paraserianthes falcataria)와 활엽수(Shoreasp) 골목에서 두 개의 Ganoderma lucidum 균주의 자실체 발생과 성장을 연구하고 자실체의 organic germanium 과 crude ganoderic acid 함량을 측정하기 위함이다. 두 개의 Ganaderma lucidum중 하나는 인도네시아 본지에서 분리한 것이고 또 다른 하나는 Fungi Perfecti, USA에서 구입한 것을 사용하였다. 일반적으로 활엽수에서 생성된 자실체의 농도가 침엽수에서 자란 것보다 훨씬 높다. 두 종류의 골목 모두에서 상업용 균주에서 생산된 자실체의 organic germanium 농도가 인도네시아의 균주에서 생산된 자실체의 organic germanium 농도보다 높았다. 이에 반하여 버섯 자실체의 crude ganoderic acid 농도는 두 개의 균주로부터 생산된 자실체에서 거의 같은 농도를 보였다. 인도네시아 균주는 상업용 분리균주와 비교했을 때 생산된 자실체의 총 organic germanium과 총 crude ganoderic acid의 생산성이 높았다.

• 대한수의학회지
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• 제22권2호
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• pp.121-138
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• 1982

The present study was conducted in order to investigate the role of yeast-like fungi in bovine mastitis. Attempts were made to isolate and identify yeast-like fungi from the milk from normal udders and those with clinical or subclinical mastitis and from feces. Also incuded in the study were trials for the pathogenicity of the isolates for laboratory animals and efficacy of an anti-fungal drug for the treatment of mastitis. A total of 133 isolates of yeast-like fungi was made from milk and feces and they were identified as Candida (C.) albicans (5 isolates), C. krusei (63 isolates), C. tropicalis (27 isolates), Torulopsis (T.) glabrata (10 isolates), Rhodotourla sp. (6 isolates), Hansenula sp. (6 isolates) and Pichia sp. (1 isolate). Sixty seven strains of yeast-like fungi were isolated from the milk of 64 quarters (4.3% of quarters examined) of 55 cows (14.3% of cows examined). C. krusei, C. tropicalis, C. pseudotropicalis, C. parapsilosis and T. glabrata were isolated as the causative agents from 20 quarters (1.3% of quarters examined) with clinical mastitis. C. krusei, C. tropicalis, C. albicans, C. pseudotropicalis, T. glabrata, Rhodotorula sp. and Hansenula sp. were isolated as the causative agents from 22 quarters (1.5% quarters examined) with subclinical mastitis. C. tropicalis, C. krusei, T. glabrata and Rhodotorula sp. were isolated as the contaminants from 22 normal quarters (1.5% of quarters examined). C. krusei, C albicans, C. tropicalis, C. pseudotropicalis, C. parapsilosis, T. glabrata, Hansenula sp., Rhodotorula sp. and Pichia sp. were isolated as the contaminants from feces and all of the species except Pichia sp. were isolated from milk of the same cows at the some time. Intramammary infusion of nystatin was effective for the treatment of mastitis caused by C. albicans, C. krusei, C. tropicalis, C. pseudotoropicalis, C. parapsilosis, T. glabrata and Rhodotorula sp. C. albicans, C. krusei, C. tropicalis, C. pseudotropicalis, T. glabrata, Hamsenula sp. and Pichia sp. were pathogenic for rat but C. parapsilosis and Rhodotorula sp. were not.

• Journal of Microbiology and Biotechnology
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• 제31권11호
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• pp.1526-1532
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• 2021

Suaeda australis, Phragmites australis, Suaeda maritima, Suaeda glauca Bunge, and Limonium tetragonum in the Seocheon salt marsh on the west coast of the Korean Penincula were sampled in order to identify the endophytes inhabiting the roots. A total of 128 endophytic fungal isolates belonging to 31 different genera were identified using the fungal internal transcribed spacer (ITS) regions and the 5.8S ribosomal RNA gene. Fusarium, Paraconiothyrium and Alternaria were the most commonly isolated genera in the plant root samples. Various diversity indicators were used to assess the diversity of the isolated fungi. Pure cultures containing each of the 128 endophytic fungi, respectively, were tested for the plant growth-promoting abilities of the fungus on Waito-C rice germinals. The culture filtrate of the isolate Lt-1-3-3 significantly increased the growth of shoots compared to the shoots treated with the control. Lt-1-3-3 culture filtrate was analyzed and showed the presence of gibberellins (GA₁ 2.487 ng/ml, GA₃ 2.592 ng/ml, GA₉ 3.998, and GA₂₄ 6.191 ng/ml). The culture filtrate from the Lt-1-3-3 fungal isolate produced greater amounts of GA₉ and GA₂₄ than the wild-type Gibberella fujikuroi, a fungus known to produce large amounts of gibberellins. By the molecular analysis, fungal isolate Lt-1-3-3 was identified as Gibberella intermedia, with 100% similarity.

• 한국식물병리학회지
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• 제14권6호
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• pp.700-704
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• 1998

A total of 26 and 55 isolates of fungi were isolated from corn and wheat samples collected from different markets in Korea, respectively. The number of Penicillium isolates from corn and wheat was 9 and 33, respectively. The Penicillium species isolated from corn were P. chrysogenum (3 isolates) and P. oxalicum (6 isolates), and from wheat were P. aurantiogriseum (16 isolates), P. citrinum (1 isolate), P. commun (4 isolates), P. griseofulvum (1 isolate), P. verrucosum (7 isolates), and P. viridicatum (4 isolates). Production of major mycotoxins in the yeast extract sucrose medium cultures of Penicillium isolates was analysed. Penicillium cultures were extracted with chloroform and purified by thin-layer chromatograhy (TLC), and high performance liquid chromatography (HPLC). Among 9 isolates of Penicillium from corn, 2 isolates of P. chrysogenum produced patulin, 1 isolate of the fungus produced patulin and citrinin, 2 isolates of P. oxalicum produced penicillic acid, 4 isolates produced pencillic acid and griseofulvin. Of the 33 isolates of Penicillium from wheat, 6 isolates of P. aurantiogriseum produced patulin, 8 isolates produced penicillic acid, 1 isolate produced patulin and penicillic acid, 1 isolate of P. citrinum produced citrinin and patulin, 2 isolates of P. commun produced brefeldin A and patulin, 1 isolate of P. griseofulvum produced brefeldin A, griseofulvin and patulin. Five isolates of P. verrucosum produced patulin, 1 isolate of the fungus produced penicillic acid, and 3 isolates of P. viridicatium produced penicillic acid.

• Mycobiology
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• 제28권3호
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• pp.127-129
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• 2000

During the study of soil mycoflora in Jinju-shi in 1997, a dematiaceous hyphomycete, Gonytrichum macrocladum, was isolated using the soil dilute plating method. The isolate was recovered with very low frequencies and recorded for the first time in Korea. illustrated descriptions are presented for the isolate examined in the present study.

• Mycobiology
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• 제47권4호
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• pp.430-440
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• 2019

Fungal contamination of built-in furniture is a frequent problem in Korea when new apartment is built. However, domestic information on the contaminating fungi is very limited. This study was conducted to isolate, identify and characterize the fungi of the problem in one of the apartment houses where the fungi were claimed in the built-in furniture before the house owner moves in. Fungi present in the furniture installed in a main room, dress room, and kitchen side were visually and microscopically confirmed and purely isolated on PDA. The isolated fungi were identified by analyzing the morphological characteristics and nucleotide sequence of the ITS, calmodulin gene, and TEF-1α gene. Aspergillus creber, A. niger, A. pseudoglacus, A. ruber, Cladosporium perangustum and Penicillium commune were identified. Four out of the six fungal species were positive for at least one enzyme in six kinds of extracellular enzyme assays. When these four species (A. creber, A. niger, C. perangustum and P. commune) were inoculated onto four kinds of wood chips of furniture materials, they were able to colonize all of the wood chips. Their settlement was better at 95% humidity condition than at 30% humidity condition. Among the four species, C. perangustum caused the darkest discoloration and secreted the most number of extracellular enzymes. The four species were re-isolated from the colonized wood chips and confirmed as the problematic fungi in the built-in furniture.

• 복식
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• 제53권5호
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• pp.23-31
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• 2003

The Purpose of this study was to isolate and identify fungi found in excavated clothes dating on the 16th century. As a result of microscoping with LPCB staining, seven isolated strains were classified-Acremonium strictum, Acremonium kiliense, Fonsecaea sp., Candida sp., Trichoderma sp., Cladosporium spp., Penicillium spp.. In the process of isolation and identification of fungi by the sort of textile, more fungi were found in cotton, cotton batt and silk than in ramie and hemp, suggesting that hemp has antibacterial characteristics due to the presence of lignin. In the Comparison washed samples with unwashed ones, there were more kinds of fungi in washed samples. In the cellulase activity test, Acremonium kiliense had the highest level cellulase activity, suggesting that Acremonium killiense has an important Influence on damage of textiles.

• The Plant Pathology Journal
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• 제23권4호
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• pp.287-294
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• 2007

Endophytic fungi were isolated from surface sterilized root tissues of Aralia elata and Aralia continentalis, collected from farmer's field in Chungnam province, Republic of Korea, in 2005. Based on ITS sequence analysis, 24 fungal genera were characterized from 359 isolates, belonging to 22 Ascomycota, 1 Glomeromycota and 1 Oomycota. Strumella, Rhizopycnis and Entrophospora in A. elata and Rhizopycnis and Leptosphaeria in A. continentalis were the most abundant taxa. Out of 24 genera, Entrophospora, Leptodontidium, Neoplaconema, Paraconiothyrium, Rhizopycnis, Strumella and Tumularia were new to Korea. A total of 110 isolates were tested for antifungal activities against six plant pathogenic fungi. Out of these, 39 isolates showed antifungal activity against at least one plant pathogenic fungi. Four isolates of Pyrenochaeta, 1 isolate of Entrophospora and 1 unidentified fungus strongly inhibited the growth of six plant pathogenic fungi.

• Mycobiology
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• 제34권4호
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• pp.185-190
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• 2006

Endophytic fungi were isolated from healthy leaf and root samples of Taraxacum coreanum. Of the 72 isolates recovered, 39 were from leaves and 33 from roots with an isolation frequency of 54% and 46%, respectively. Based on ITS sequence analysis, 72 isolates were classified into 19 genera of which 17 were under the phylum Ascomycota and 2 were under Basidiomycota. Diverse genera were found and Alternaria, Cladosporium, Fusarium and Phoma were dominant. Out of 19 genera, Apodus, Ceriporia, Dothideales, Leptodontidium, Nemania, Neoplaconema, Phaeosphaeria, Plectosphaerella and Terfezia were new to Korea. Seventy two isolates were screened for antifungal activity, of which 10 isolates (14%) were found active at least against one of the tested fungi. Isolate 050603 had the widest antifungal spectra of activity, and isolates 050592 and 050611 were active against three plant pathogenic fungi.

• 한국균학회지
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• 제51권4호
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• pp.265-276
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• 2023

본 연구는 담수 환경에서 분리한 곰팡이의 특성을 알아보기 위해 효소 활성을 평가하였다. 40개의 곰팡이들은 다양한 담수 시료로부터 분리되었으며, 계통분석을 통해 동정하였다. 실험에 사용된 균주들은 최근에 국내에 보고되었거나, 아직 보고되지 않은 종으로서 이에 대한 특성 정보가 거의 알려지지 않았다. 본연구에서는 40개 균주를 대상으로 protease, amylase, lipase, cellulase, laccase, chitinase의 효소에 대해서 활성을 검정하였다. 대부분의 균주가 laccase 활성을 보였으며, protease, amylase 순으로 높게 나타났다. 담수 환경에서의 효소 활성 정보는 이들의 생태적 역할을 이해하고 산업적으로 활용하는데 기여할 수 있을 것이다.