• Journal of Mushroom
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• v.17 no.4
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• pp.179-184
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• 2019

This study was conducted to investigate the diversity of culture-dependent fungal species in the sawdust media of Lentinula edodes. A total of 405 fungi were isolated from the specimens and identified to belong to 24 genera and 42 species. Among the identified 42 species of fungi, 26.2% belonged to Penicillium sp., 9.5% belonged to Trichoderma sp., and 64.3% belonged to others. Especially, Trichoderma harzianum, which is a causal agent of fungal disease in mushroom, was found on all the farms, and showed the highest frequency among the identified fungi. Community analysis showed that the fungal diversity patterns of the samples were similar to each farm and many kinds of fungi existed in the sawdust media at high levels. These results showed that the management of internal environments would be required for the stable cultivation of Lentinula edodes.

• Journal of Ginseng Research
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• v.36 no.2
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• pp.211-217
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• 2012

Endophytic fungi were isolated from various tissues (root, stem, petiole, leaf, and flower stalk) of 3- and 4-year-old ginseng plants (Panax ginseng Meyer) cultivated in Korea. The isolated endophytic fungi were identified based on the sequence analysis of the internal transcribed spacer (ITS), 1-5.8-ITS 2. A morphological characterization was also conducted using microscopic observations. According to the identification, 127 fungal isolates were assigned to 27 taxa. The genera of Phoma, Alternaria and Colletotrichum were the most frequent isolates, followed by Fusarium, Entrophospora and Xylaria. Although 19 of the 27 taxa were identified at the species level, the remainder were classified at the genus level (6 isolates), phylum level (Ascomycota, 1 isolate), and unknown fungal species (1 isolate). Endophytic fungi of 13 and 19 species were isolated from 3- and 4-year-old ginseng plants, respectively, and Phoma radicina and Fusarium solani were the most frequently isolated species colonizing the tissues of the 3- and 4-year-old ginseng plants, respectively. The colonization frequency (CF%) was dependant on the age and tissue examined: the CFs of the roots and stems in the 3-year-old ginseng were higher than the CF of tissues in the 4-year-old plants. In contrast, higher CFs were observed in the leaves and petioles of 4-year-old plants, and endophytic fungi in the flower stalks were only detected in the 4-year-old plants. In conclusion, we detected diverse endophytic fungi in ginseng plants, which were distributed differently depending on the age and tissue examined.

• The Korean Journal of Mycology
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• v.45 no.4
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• pp.304-318
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• 2017

While evaluating fungal diversity in fecal and freshwater samples in Korea, three fungal strains, CNUFC-GHD83-1, CNUFC-RD8126, and CNUFC-YR2-1, were isolated from specific habitats including grasshopper and rat feces, and freshwater samples in Korea. On the basis of the morphological characteristics and phylogenetic analysis of the internal transcribed spacer (ITS) and 28S rDNA, the isolates CNUFC-GHD83-1, CNUFC-RD8126, and CNUFC-YR2-1 were identified as Albifimbria terrestris, Cephaliophora tropica, and Mariannaea aquaticola, respectively. These species have not been previously described in Korea.

• Journal of Applied Biological Chemistry
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• v.50 no.4
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• pp.221-226
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• 2007

The high molecular-weight mucilage extracted and purified from cactus fruit of Opuntia ficus-indica var. Saboten was degraded by the cell-free culture filtrate of a fungus isolated from soil. TLC analysis of the polymeric mucilage after incubation with the fungal culture filtrate confirmed its degradation. When the degradation products were tested for their qualitative reactions with ninhydrin and phenol-sulfuric acid, only phenol-sulfuric acid gave positive development, and ninhydrin did not show any observable color reaction. This coloring reaction suggested the presence of a carbohydrate without an amino group within the mucilage. Analyses by HPLC and liquid gel permeation chromatography on sephadex G-100 also provided additional information on degradation of the mucilage by the fungal culture filtrate. The sequences of ITS-5.8S rDNA from the fungal isolate that was cultivated for the preparation of mucilage-degrading enzyme showed 99% similarity to those of Pestalotiopsis aquatica.

• Mycobiology
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• v.30 no.2
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• pp.76-81
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• 2002

Aquatic fungi from four brackish water lakes; Edku, Burullus and Manzala lakes which are located at the northern region of Egypt and Qarun lake that located in El-Fayoum city are reported in this manuscript. Twenty-nine fungal species which belong to 19 genera of aquatic fungi were recovered from water samples collected from the studied lakes. The most frequently isolated fungal species were Chytridium conferrop, Allomyces throughout and Rhizoclosmatium globosum. Thraustochytrium amoeboidum and Leptolegniella exoosporus have a moderately occurrence frequency. The maximum fungal count of recovered aquatic fungi was recorded in Burrullus lake followed by EdKu, Manzala and Qarun lakes. This study was extended to test the ability of six selected aquatic fungi(Brevilegniella keratinophila, Blastocladiella cystogena, Chytridium conferrop, Entophlyctis variabilis, Schizochytrium mangrovei and Thraustochytrium rosin), to uptake the radionuclide from their culture medium as a step to biologically treat the waste water or solution with radio-cesium and radio-cobalt. Fifty seven of Cs-137 and 35% of Co-60 could be removed from liquid waste by the selected aquatic fungi.

• Mycobiology
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• v.42 no.4
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• pp.317-321
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• 2014

Fruiting bodies similar to those of the ascomycete fungi Podostroma cornu-damae and Cordyceps militaris were collected from Mt. Seunghak in Busan, Korea on August 21, 2012. The fruiting bodies were cylindrical, with tapered ends and golden red in color. The fruiting bodies contained abundant conidiophores bearing single-celled conidia, but no perithecia or asci. Pure culture of the fungal isolates was obtained through single-spore isolation. Analyses of morphological characteristics, including conidia shape, and phylogenetic traits, using internal transcribed spacer sequences, showed that these isolates belonged to the species Simplicillium lanosoniveum. Although this fungal species is known to be mycoparasitic, the isolates obtained in this study were unable to infect fungi. However, silkworms (Bombyx mori) inoculated with the fungal isolates died during the larval or pupal stages, as has been shown for the strongly entomopathogenic fungus Beauveria bassiana. This study is the first report of the entomopathogenicity of S. lanosoniveum and indicates its potential for use in biological control of insects.

• 한국균학회소식:학술대회논문집
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• 2014.10a
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• pp.50-50
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• 2014

In this study, we used pyrosequencing method to analysis of soil fungal communities on the Ulleungdo and Dokdo islands. 768 operational taxonomic units (OTUs) were analyzed from the Ulleungdo sample and 640 OTUs and 382 OTUs were analyzed from the Dongdo and Seodo samples, respectively. Compared to the species richness of Ulleungdo and the Dokdo sample, the Ulleungdo sample was higher than in the Dongdo and Seodo samples. Species diversity was much the same. The phylum Basidiomycota was dominant in the Ulleungdo sample, while the phylum Ascomycota was dominant in the Dongdo sample.

• Mycobiology
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• v.46 no.4
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• pp.328-340
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• 2018

While evaluating fungal diversity in freshwater, grasshopper feces, and soil collected at Dokdo Island in Korea, four fungal strains designated CNUFC-DDS14-1, CNUFC-GHD05-1, CNUFC-DDS47-1, and CNUFC-NDR5-2 were isolated. Based on combination studies using phylogenies and morphological characteristics, the isolates were confirmed as Ascodesmis sphaerospora, Chaetomella raphigera, Gibellulopsis nigrescens, and Myrmecridium schulzeri, respectively. This is the first records of these four species from Korea.

• Journal of Species Research
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• v.3 no.2
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• pp.123-126
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• 2014

We present a list of the beetle species associated with the oyster mushroom, Pleurotus ostreatus Fr. in the birch forest of Onon-Balj National Park in Mongolia. A total of 21 species in seven families were found in this fungus, including fungivorous species: Litargus japonicus Reitter and Mycetophagus antennatus (Reitter) (Mycetophagidae), Pseudamblyopus similis (Lewis) (Erotylidae), and Lordithon sp. (Staphylinidae) etc. Both adult and larval forms are presented in the checklist along with the type of fungal association, of which approximately 52 percent are obligate mycetobionts. The main trends of the ecological and trophic specialization of the mycetophilic beetles are discussed.

• Mycobiology
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• v.42 no.4
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• pp.416-421
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• 2014

In this study, we examined arbuscular mycorrhizal fungal (AMF) community structure colonizing field-cultivated ginseng roots according of different ages, such as 1- to 5-year-old plant, collected from Geumsan-gun, Korea. A total of seven AMF species namely, Funnelliformis caledonium, F. moseae, Gigaspora margarita, Paraglomus laccatum, P. occultum, Rhizophagus irregularis, and Scutellospora heterogama were identified from the roots using cloning, PCR-restriction fragment length polymorphism and sequence analysis of the large subunit region in rDNA. AMF species diversity in the ginseng roots decreased with the increase in root age because of the decreased species evenness. In addition, the community structures of AMF in the roots became more uniform. These results suggest that the age of ginseng affects mycorrhizal colonization and its community structure.