• The Korean Journal of Mycology
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• v.21 no.1
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• pp.38-50
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• 1993

Four plant (Sorghum bicolor, Cassia mimosoides var. nomame, Sesamum indicum and Glycine soja) were cultivated at the pots including the soils containing arbuscular mycorrhizal fungi and were also investigated with the colonizations and productions of arbuscular mycorrhizal fungi. Whereas the colonizations of arbuscular mycorrhizal fungi continuosly increased on the roots until 50 days, the productions of arbuscular mycorrhizal fungal spores were fluctuated with the terms of 30 days after inoculated. This indicated that the colonizations on the roots were not correlated with productions of arbuscular mycorrhizal fungal spores. Also, the various soils collected were applied to this technique by using pot cultures. Out of 82 various soils collected, the spore productions of arbuscular mycorrhiaze were observed only from 42 soils. The spores cultured under artificial conditions were identified to 15 species with four genera. The spore productions of arbuscular mycorrhizal fungi using this technique would be considered to be related to the soil pH: The spore productions were found in the low pH for the species of Acaulospora and Glomus, the those near pH 7.6 for the species of some Glomus, Scutellospora and Gigaspora.

• The Korean Journal of Mycology
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• v.19 no.2
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• pp.148-155
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• 1991

Fungal ochratoxin A wes extracted and measured from the Korean traditional and fermented soybean foodstuffs (12 samples of Maeju, 28 samples of Dwangjang and 28 samples of Kangjang) collected from the whole nation wide regions. The various fungi were isolated from the foodstuffs and they were also examined whether the isolates produce ochratoxin A (OA) under the artificial conditions or not. Determinations of OA produced by the fungi were done with the antibody-attached CIA method, which was accurate or sensitive at the range of 20 pg per sample with a ninty percent recovery. Out of the 222 fungal isolates, 39 isolates produced the OA under the artificial conditions, and were identified as species of Aspergillus, Penicillium, Paecilom­yces or other genera. The OA detected in all soybean foodstuffs was presumed to be originated from the first fermentation step of maeju.

• The Korean Journal of Mycology
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• v.39 no.3
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• pp.155-163
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• 2011

The investigation on the species diversity for the Korean fungal flora was carried out by authors. Five species of the Agaricales recorded in the present paper are distributed Hygrocybe laeta (Pers.)P. Kummer var. laeta, Lyophyllum tylicolor (Fr.) M. Lange & Sivertsen, Clitopilus scyphoides (Fr.) Singer, var. scyphoides, Entoloma rusticoides (Gillet) Noordel. and Lactarius uyedae Singer were recorded for the first time and are described and illustrated in detail. Specimens cited here are deposited in the Herbarium Conservation Center of National Academy of Agricultural Sciences.

• The Korean Journal of Mycology
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• v.22 no.3
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• pp.216-221
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• 1994

As a part of studies on the fungal flora of Korea, authors have collected over 60 higher fungi specimens during summer season in 1993 in Mt. Hungjung which was located in eastern part of Korea. Among them two genera are new to Korea: Neobulgaria Petrak and Melastiza Boudier. Following five species are recorded for the first time in Korea: Psilocybe xeroderma Huijsm, Galerina vittaeformis (Fr.) Singer var. vittaeformis f. vittaeformis (Fr.) Singer, Gyromitra infula (Schaeffer ex Fries) Quelet, Neobulgaria pura (Fries) Petrak and Melastiza chateri (W. G. Smith) Boudier, for which Korean common names are designated in this present paper. All color names and terms within quotation marks are taken from Kornerup and Wanscher 1983, Methuen handbook of colour. The specimens are all deposited in the RDAGB's herbarium.

• Mycobiology
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• v.45 no.4
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• pp.240-254
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• 2017

Cereal grains are the most important food source for humans. As the global population continues to grow exponentially, the need for the enhanced yield and minimal loss of agricultural crops, mainly cereal grains, is increasing. In general, harvested grains are stored for specific time periods to guarantee their continuous supply throughout the year. During storage, economic losses due to reduction in quality and quantity of grains can become very significant. Grain loss is usually the result of its deterioration due to fungal contamination that can occur from preharvest to postharvest stages. The deleterious fungi can be classified based on predominance at different stages of crop growth and harvest that are affected by environmental factors such as water activity ($a_w$) and eco-physiological requirements. These fungi include species such as those belonging to the genera Aspergillus and Penicillium that can produce mycotoxins harmful to animals and humans. The grain type and condition, environment, and biological factors can also influence the occurrence and predominance of mycotoxigenic fungi in stored grains. The main environmental factors influencing grain fungi and mycotoxins are temperature and $a_w$. This review discusses the effects of temperature and $a_w$ on fungal growth and mycotoxin production in stored grains. The focus is on the occurrence and optimum and minimum growth requirements for grain fungi and mycotoxin production. The environmental influence on aflatoxin production and hypothesized mechanisms of its molecular suppression in response to environmental changes are also discussed. In addition, the use of controlled or modified atmosphere as an environmentally safe alternative to harmful agricultural chemicals is discussed and recommended future research issues are highlighted.

• Journal of Microbiology and Biotechnology
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• v.33 no.4
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• pp.543-551
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• 2023

In this study, five endophytic fungi belonging to the Aspergillus and Alternaria genera were isolated from Lagopsis supina. The antimicrobial activity of all fungal fermented extracts against Staphylococcus and Fusarium graminearum was tested using the cup-plate method. Among them, Aspergillus ochraceus XZC-1 showed the best activity and was subsequently selected for large-scale fermentation and bioactivity-directed separation of the secondary metabolites. Four compounds, including 2-methoxy-6-methyl-1,4-benzoquinone (1), 3,5-dihydroxytoluene (2), oleic acid (3), and penicillic acid (4) were discovered. Here, compounds 1 and 4 displayed anti-fungal activity against F. graminearum, F. oxysporum, F. moniliforme, F. stratum, Botrytis cinerea, Magnaporthe oryzae, and Verticillium dahlia with diverse MIC values (128-512 ㎍/ml), which were close to that of the positive control antifungal, actidione (64-128 ㎍/ml). Additionally, compounds 1 and 4 also exhibited moderate antibacterial activity against S. aureus, Listeria monocytogenes, Escherichia coli, and Salmonella enterica, with low MIC values (8-64 ㎍/ml). Moreover, compounds 1 and 4 displayed selective cytotoxicity against cancer cell lines as compared with the normal fibroblast cells. Therefore, this study proposes that the endophytic fungi from L. supina can potentially produce bioactive molecules to be used as lead compounds in drugs or agricultural antibiotics.

• The Korean Journal of Mycology
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• v.36 no.1
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• pp.1-8
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• 2008

To investigate the mycoflora of indoor air in a Sogokju, traditional rice wine, factory, fungi were sampled and analyzed from the air of several rooms in the factory using an Anderson air sampler and from two kinds of Nuruk. Twelve fungal species belonging to the genera of Aspergillus, Penicillium, Gibberella, Cladosporium, and Talaromyces were isolated. Species belonging to Aspergillus and Penicillium genera were the major species. Seven different species of Penicillium were isolated from each different room of the factory. The Aspergillus species found from indoor air of the factory was also found from Nuruk. Rhizopus sp. was commonly isolated from Nuruk but not from indoor air of the factory. This is first report of fungi present in indoor air of a traditional rice wine factory in Korea.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.18 no.1
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• pp.11-19
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• 2008

Mean levels of airborne bacteria, airborne fungi, temperature, relative humidity and carbon dioxide in total 69 general offices were $426({\pm}83)\;cfu/m^3$, $234({\pm}125)\;cfu/m^3$, $25.9({\pm}1.3)\;^{\circ}C$, $57.7({\pm}8.6)\;%$, $422({\pm}38)\;ppm$, respectively. The I/O ratio of airborne bacteria and fungi was over 1 and there was no significant difference among temperature, relative humidity and carbon dioxide in total 69 general offices. In construction period, a concentration of airborne bacteria and fungi was significantly highest in general offices constructed under one year and over three years since construction, respectively (p<0.05). The concentration of airborne fungi in general offices located at basement was significantly higher than those located at ground (p<0.05). No significant difference of airborne bacteria and fungi in general offices was found regardless of installation of HVAC system (p>0.05). The dominant bacterial genera identified in general offices was Staphylococcus, followed by Micrococcus, Bacillus, and Corynebacterium while usarium, Penicillium, Aspergillus, Alternaria, Rhizopus and Mucor were identified as dominant fungal genera in general offices.

• Journal of Food Hygiene and Safety
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• v.26 no.4
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• pp.361-365
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• 2011

To evaluate the indoor air quality of food manufacturing plants, the presence of viable bacteria and fungi was assessed in the indoor air of the facilities at which 9 food items were manufactured. Air samples were collected from the general zone, low clean zone and clean zone of each factory with an air sampler, in combination with plate counts agar using for bacteria, and dichloran-glycerol agar for fungi. The samples were incubated at $25^{\circ}C$ for 4 to 7 days. After culture, the colony forming units (CFU) on each plate were counted and corrected with a positive hole conversion table. The average concentration of bacteria was $2.2{\times}10^3\;CFU/m^3$ in the general zone, $1.2{\times}10^3\;CFU/m^3$ in the low clean zone and $7.3{\times}10^2\;CFU/m^3$ in the clean zone. The average concentration of fungal microbes was $2.5{\times}10^3\;CFU/m^3$ in the general zone, $2.6{\times}10^3\;CFU/m^3$ in the low clean zone, and $2.0{\times}10^2\;CFU/m^3$ in the clean zone. No meaningful differences were detected between the general zone and the low clean zone, but the clean zone had significantly lower concentrations than the other zones. Additionally, the identification of the fungi was performed according to morphological method using a giant culture and slide culture. The fungi were identified as belonging to 18 genera, and the genera Cladosporium(33%), Penicillium(29%) and Aspergillus(26%), predominated. Aspergillus isolates were identified to species level, and A. ochraceus, a mycotoxigenic species, was identified. As part of the effort to control the quality of the indoor air of food manufacturing plants, our results show that continued studies are clearly warranted.

• Environmental Analysis Health and Toxicology
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• v.5 no.3_4
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• pp.29-36
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• 1990

Aflatoxins, produced by strains of Aspergillus flavus and Aspergillus parasiticus, can be found worldwide in corn, barley, peanuts, and other commodities. Among this group of toxins, aflatoxin B$_1$was realized to be one of the most potent environmental carcinogens, mutagens and teratogens. It is routinely monitored by methods such as thin layer chromatography, liquid chromatography, fluorodensitometric technique and radioimmunoassay. However, these assays are expensive, necessitate radioactive reagents, and require overnight incubation. In this study, the determination of fungal flora in several sorts cereals has been carried out in order to obtain an appropriate information of the population of fungi. The quantitative analysis of aflatoxin B$_1$has been carried out by High Performance Liquid Chromatography (HPLC) method and Enzyme Linked Immunosorbent Assay (ELISA). The results were summarized as follow: 1) From the 100 samples,313 colonies of fungi were isolated. Among the 313 colonies, 274 were possible to identify into 11 genera. The identified genera were Aspergillus Penicillium, Mucor, Rhizopus, Alternaria, Cladosorium, Fusarium, Circinella, Chrysosporium, Paecilomyces and Phoma. 2) Six of Aspergillus flavus were aflatoxin-producing strains. Aspergillus flavus isolated from sample barleys was contained the highest content (21.8 $\mu\textrm{g}$/ml) of aflatoxin B$_1$. 3) The yield of aflatoxin B$_1$-oxime compound was appromately 75%. Aflatoxin B$_1$-oxime-Human serum albumin was approved by formal consent as complete antigen. 4) Direct competitive ELISA permitted detection of 0.15 ng levels. In the quantitative microanalysis, ELISA was superior to HPLC method.