• Journal of Life Science
• /
• v.23 no.2
• /
• pp.290-298
• /
• 2013

The genus Mycobacterium includes crucial animal and human pathogens such as Mycobacterium tuberculosis, Mycobacterium leprae, and Mycobacterium bovis. Although it is important to understand the genetic basis for their virulence and persistence in host, genetic analysis in mycobacteria was hampered by a lack of sufficient genetic tools. Therefore, many functional vectors as molecular genetic tools have been designed for understanding mycobacterial biology, and the application of these tools to mycobacteria has accelerated the study of mechanisms involved in virulence and gene expression. To overcome the pre-existing problems in genetic manipulation of mycobacteria, this paper reports new vector systems as effective genetic tools in Mycobacterium smegmatis. Three vectors were developed; pKOTs is a suicide vector for mutagenesis containing a temperature-sensitive replication origin (TSRO) and the sacB gene encoding levansucrase as a counterselectable marker. pMV306lacZ is an integrative lacZ transcriptional fusion vector that can be inserted into chromosomal DNA by site-specific recombination. pTnMod-OKmTs is a minitransposon vector harboring the TSRO that can be used in random mutagenesis. It was demonstrated in this study that these vectors effectively worked in M. smegmatis. The vector systems reported here are expected to successfully applicable to future research of mycobacterial molecular genetics.

• Food Science of Animal Resources
• /
• v.29 no.6
• /
• pp.659-667
• /
• 2009

Casein is considered to be the main source of protein in milk; therefore, many studies have been conducted to identify casein-derived bioactive peptides and their physiological effects. Casein is inactive within the parent protein but can be liberated by various proteases and enzymatic hydrolysis during microbial fermentation and gastrointestinal digestion. Once absorbed, casein exhibits different bioavailabilities in the body. Specifically, casein-derived peptides function as angiotensin converting enzyme (ACE) inhibitor in the cardiovascular system; thus, they are expected to reduce and prevent hypertension. Additionally, casein-derived peptides behave as opioid-like peptides in the nervous system, which impacts relaxation. These peptides are also expected to modulate various aspects of immune functions. Finally, caseinophosphopeptide (CPP) and glycomacropeptide (GMP) may exhibit a number of nutritional effects such as the absorption of calcium, iron or zinc. Many studies have been conducted to evaluate casein-derived peptides due to their multifunctional properties and the results of these studies have contributed to the development of a wide variety of functional dairy products. The purpose of this paper was to review the generation of bioactive peptides, their absorption and metabolism, and their specific bioactive effects.

• Journal of the Korean Institute of Intelligent Systems
• /
• v.22 no.6
• /
• pp.722-728
• /
• 2012

Urban sewer systems has a limitation of capacity of rainwater storage and problem of occurrence of untreated sewage, so adopting a storage facility for sewer flooding prevention and urban non-point pollution reduction has a big attention. The Korea Ministry of Environment has recently introduced a new concept of "multi-functional storage facility", which is crucial not only in preventive stormwater management but also in dealing with combined sewer overflow and sanitary sewer discharge, and also has been promoting its adoption. However, reserving a space for a single large-scale storage facility might be difficult especially in urban areas. Thus, decentralized construction of small- and midium-sized storage facilities and its operation have been introduced as an alternative way. In this paper, we propose a model predictive control scheme for an optimized operation of distributed storage facilities and sewer networks. To this aim, we first describe the mathematical model of each component of networks system which enables us to analyze its detailed dynamic behavior. Second, overflow locations and volumes will be predicted based on the developed network model with data on the external inflow occurred at specific locations of the network. MPC scheme based on the introduced particle swarm optimization technique then produces the optimized the gate setting for sewer network flow control, which minimizes sewer flooding and maximizes the potential storage capacity. Finally, the operational efficacy of the proposed control scheme is demonstrated by simulation study with virtual rainstorm event.

• 재활복지
• /
• v.22 no.4
• /
• pp.103-121
• /
• 2018

For knowing the effects of aging on conversational skills in daily communication, this paper studied for the conversational turn-taking skills, working memory and naming ability on healthy elderly adults over 65 ages. 85 elderly adults participated in this study, which divided into four groups by ages. Speech samples were collected in natural conversation. Memorization of numbers, mental calculation, repetition of words were administered for working memory test. K-BNT was used for the naming ability. One-way ANOVA analysis was used for the comparison of conversational turn-taking skills among four groups. We analyzed the correlation between conversational skills, working memory and naming ability. The results were as follows: first, there were a significant difference in conversational turn-taking skills by age, but not by gender. There was a significant difference in 'Turn-Taking Frequency' and 'Total Utterance Frequency' among four groups. The same results were shown in the scores of females within three groups(exclude groups over 85D)(p<.01). Second, there was a significant correlation between 'rates of maintenance' and 'naming ability'. In addition, it was found that the naming test predicted 'rates of maintenance' skills. The results of this study suggest that word-retrieval ability will be helpful to enhance functional communication skills in aging old adults.

• Journal of the Korea Institute of Construction Safety
• /
• v.1 no.1
• /
• pp.22-30
• /
• 2018

Construction accidents in Korea are continuously increasing. Safety signs with high visibility color can diminish such accidents in dangerous places. In order for safety color to be more effective as a global communication means in construction sites, it needs to be checked out whether safety color standard reflect the characteristics of construction industry such as worker or work environment. This paper compared safety color standards among Korea, the US, the UK, and Australia. First, blue color was included in all, which should be corrected considering aged workers increasing in construction industry. Because with age, the ability to distinguish between blue, purple, and gray colors decreases. Second, Korea, which has only single code designated to safety colors, should find alternatives like tolerance to be applicable to a variety of light environments on construction sites, as the contrast which affect the visibility may decrease in dark conditions.

• Journal of the Korean School Mathematics Society
• /
• v.24 no.1
• /
• pp.83-105
• /
• 2021

In this paper, we analyzed the misconceptions and errors incurred during factorization learning. We also examined whether online individualization classes had a positive effect on students' mathematical achievement. The experiment was conducted for 4 weeks (16 times in total) on middle school juniors in rural areas of Gyeonggi Province, where the influence of private extra education was small. In the class, the 'Google Classroom' was used as a LMS, the video lecture was uploaded to YouTube, and the teacher interacted with the students through "Zoom" and "Facetalk". In the online class situation, students' assignments and test answers were checked in real time through 'Google Classroom', and immediate feedback was provided to the experimental class group's students. However, for the control group students, feedback was provided only to those who desired. A total of 7 achievement evaluations were conducted in the order of pre-test, formative evaluation (5 times), and post-test to confirm the change in students' ability improvement and achievement. Through the formative evaluation analysis, it was possible to grasp the types of errors and misconceptions that occured during the factorization process. Students' errors were divided into four types: theorem or definition distortion error, functional errors such as calculation, operation, and manipulation, errors that do not verify the solution, and no response. As a result of ANCOVA, the two groups did not show any difference from the 1st to 4th formative assessment. However, the 5th formative assessment and post-test showed statistically significant differences, confirming that online individualization classes contributed to improvemed achievement.

• Journal of Plant Biotechnology
• /
• v.45 no.4
• /
• pp.289-298
• /
• 2018

The Biosafety Division of the National Academy of Agricultural Science has developed a 'Crop Composition DB' that provides analytical data on commercialized crops. It can be used as a reference in the 'Comparative Evaluation by Compositional Analysis' for the safety assessment of genetically modified (GM) crops. This database provides the composition of crops cultivated in Korea, and thus upgrades the data to check the extent of changes in the compositional content depending on the cultivated area, varieties and year. The database is a compilation of data on the antioxidant, nutrient and secondary metabolite compositions of rice and capsicum grown in two or more cultivation areas for a period of more than two years. Data analysis was conducted under the guidelines of the Association of Official Analytical Chemists or methods previously reported on papers. The data was provided as average, minimum and maximum values to assess whether the statistical differences between the GM crops and comparative non-GM crops fall within the biological differences or tolerances of the existing commercial crops. The Crop Composition DB is an open-access source and is easy to access based on the query selected by the user. Moreover, functional ingredients of colored crops, such as potatoes, sweet potatoes and cauliflowers, were provided so that food information can be used and utilized by general consumers. This paper introduces the feature and usage of 'Crop Composition DB', which is a valuable tool for characterizing the composition of conventional crops.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.20 no.3
• /
• pp.91-99
• /
• 2019

Naval Ships have a number of requirements related to performance. Naval ship acquisition takes a long time from initial planning to delivery, and various products such as drawings and reports are created. In complex systems, such as naval ships, it is difficult to maintain the required traceability through these outputs. The basic design of the ship is an important step to determine the specifications, performance, and equipment of the ship. It is necessary to apply the systematic requirements management process. The basic design manages the requirements in accordance with the systems engineering-based technical review process, but the actual system architecture design process is not presented. The traceability between the requirements and the functional and physical architectures of components is unclear. This paper examined how to design the system architecture required for specification and system design, and the design results were verified using SE-based technical review process for requirements management. A step-by-step process for designing a submarine system architecture is presented and verified using the SE technical review. This facilitates the specification of the requirements and system architecture design, and supports traceability management and verification of the requirements. The proposed process can be applied in various ships including submarines.

• 재활복지
• /
• v.18 no.4
• /
• pp.221-235
• /
• 2014

Recently, the frequency of stroke disease is increased due to the rapid aging population, and is contributed to the major occurrence factors of the posteriori acquired disability. This study is about an postural change device for the control of supine posture which is an assisted equipment using in daily rehabilitation process for overcoming the disability by the aftereffects of the stoke disease. In this paper, the existing domestic and Japan postural appliances is examined and its comparison and categorization is performed according to its functions and purposes. Here, in order to control the supine posture state, the design method for advanced multi functional system is proposed, which is devised to have an unified mattress control operations of combining the bedsore prevention tube with the supine posture tilting tube. And also, in addition of an smart function, it is designed to enable to perform an RF functions such as the monitoring of the present device state, the alteration of the basic position and the control of alternative floating and supine posture. This system control hardware consists of three main parts : the sensor detection part, the motor driving /control part, and the system control part for bluetooth communication. In results, we confirmed that the system designed by this research is possible to make it practical as an advanced smart postural change device combined by IoT technology in the application field of the recent IT technology.

• Health Policy and Management
• /
• v.29 no.2
• /
• pp.206-219
• /
• 2019

This paper aims to demonstrate current health expenditure (CHE) and National Health Accounts of the years 2018 constructed according to the SHA2011, which is a manual for System of Health Accounts (SHA) that was published jointly by the Organization for Economic Cooperation and Development (OECD), Eurostat, and World Health Organization in 2011. Comparison is made with international trends by collecting and analyzing health accounts of OECD member countries. Particularly, scale and trends of the total CHE financing as well as public-private mix are parsed in depth. In the case of private financing, estimation of total expenditures for (revenues by) provider groups (HP) is made from both survey on the benefit coverage rate of National Health Insurance (by National Health Insurance Service) and Economic Census and Service Industry Census (by National Statistical Office); and other pieces of information from Korean Health Panel Study, etc. are supplementarily used to allocate those totals into functional classifications. CHE was 144.4 trillion won in 2018, which accounts for 8.1% of Korea's gross domestic product (GDP). It was a big increase of 12.8 trillion won, or 9.7%, from the previous year. GDP share of Korean CHE has already been close to the average of OECD member countries. Government and compulsory schemes' share (or public share), 59.8% of the CHE in 2018, is much lower than the OECD average of 73.6%. 'Transfers from government domestic revenue' share of total revenue of health financing was 16.9% in Korea, lower than the other social insurance countries. When it comes to 'compulsory contributory health financing schemes,' 'transfers from government domestic revenue' share of 13.5% was again much lower compared to Japan (43.0%) and Belgium (30.1%) with social insurance scheme.