• Korean Journal of Agricultural Science
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• v.50 no.2
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• pp.323-336
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• 2023

As a byproduct obtained from cheese manufacture, whey protein was developed as a functional food that contains multi-functional proteins. In this study, the biochemical activity of fermented milk prepared by fortifying whey protein with excellent physiological activity was investigated. Immunoglobulin (IgG) content was higher in 10% fortified whey protein fermented milk than in the control. The viable cell counts were 20% higher in the fermented milk with 10% fortified whey protein than in the control group. The antibacterial effect of 10% fortified whey protein fermented milk compared to the control group was shown to be effective against four pathogenic microorganisms, Escherichia coli (KCTC1039), Pseudomonas aeruginosa 530, Salmonela Typhimurium (KCTC3216), and Staphylococcus aureus (KCTC1621). The antioxidant effect by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities wasincreased two-fold in 10% fortified whey protein fermented milk compared to the control. The 10% fortified whey protein fermented milk inhibited the expression of the inflammatory cytokines (interleukin [IL]-6, tumor necrosis factor [TNF]-α, and induced nitric oxide synthase [iNOS]) in a concentration-dependent manner. In a piglets feeding test, the weight gain with 10% fortified whey protein fermented milk was increased by 18% compared to the control group, and no diarrhea symptoms appeared. Our results clearly demonstrated that 10% fortified whey protein fermented milk could be a useful functional ingredient for improving health.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.4
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• pp.264-271
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• 2019

This study was conducted to investigate the effect of whole crop rice based TMR on the meat quality of Korean Native Cattle (Hanwoo). Sixteen Hanwoo steers (average 8 months old) were divided into the control group (commercially marketed TMR) and the WCR-TMR group (whole crop rice based TMR). Feeding trials were carried out up to 30 months of age, and after slaughter, the strip loin were collected and analyzed for meat quality. There were no significant differences in proximate compositions of Hanwoo (P>0.05), and crude protein, crude fat and crude ash were 19.51~20.23 %, 11.53~11.35 % and 1.10~1.12 %, respectively. There was no significant difference in water holding capacity and cooking loss between treatments (P>0.05), but it was slightly lower in the control group. Among the various functional components in meat, β-carotene was not detected, but α-tocopherol was significantly higher in WCR-TMR group and vitamin A content in control (P<0.05). The a^⁎-value in the meat color index decreased significantly with longer storage period in the control group, whereas the WCR-TMR fed group showed no significant difference (P>0.05) according to the storage period. There were no difference between the treatments in the TBARS (2-Thiobarbituric acid reactive substances) value used as an indicator of lipid oxidation and in fatty acid content. But the control group had high myristic acid content, whereas the WCR-TMR supplemented group showed higher oleic acid and linoleic acid content. In conclusion, feeding of WCR based-TMR increased the α-tocopherol content, which is a functional ingredient in meat, and the other components were not significantly different from the control. Therefore, it was judged that there was no significant difference between WCR based and conventional TMR in meat quality characteristics.

• Journal of Korean Academy of Nursing
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• v.37 no.4
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• pp.558-567
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• 2007

Purpose: This study was to identify the effects of oropharyngeal sensory stimulation on a functional oral intake scale, ore-pharyngeal swallowing functions, and aspiration pneumonia symptoms with nasogastric tube insertion in stroke patients. Method: This study was a nonequivalent control group non-synchronized design. The subjects or the study were 32 patients who were hospitalized in Kosin Gaspel hospital. The experimental subjects were stimulated for $10{\sim}20$ minutes, 1 time per day. The intervention was implemented for 2 weeks. Result: Participants in the experimental group significantly received a higher score in ore-pharyngeal swallowing function than those in the control group. However the participants in the experimental group only got a high score in the function oralintake scale which doesn't imply a statistical significance. In addition, they didn't geta remarkably higher score in aspiration pneumonia symptoms than those in the control group. Conclusion: This study demonstrates that oropharyngeal sensory stimulation is effective in promoting recovery oro-pharyngeal swallowing function of nasogastric tube insertions in stroke patients.

• Nutrition Research and Practice
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• v.9 no.1
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• pp.37-42
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• 2015

BACKGROUND/OBJECTIVES: Red grape seeds as functional food are a good source of important bioactive components such as phenolics and antioxidants, which decrease oxidative stress that contributes to the pathogenesis of hepatotoxicity. The current study was conducted in order to evaluate the protective effect of red grape dried seeds (RGDS) on antioxidant properties, lipid metabolism, and liver and kidney functions of rats with paracetamol (750 mg/kg) induced hepatotoxicity. MATERIALS/METHODS: RGDS was added to the basal diet at 5, 10, and 20%. Thirty five adult male rats were assigned to five groups (n = 7) for a six-week feeding period; group (1) normal control, group (2) induced control, groups (3, 4, and 5) fed a diet with RGPS at different levels, 5, 10, and 20%, respectively. At the end of the feeding period, animals' blood and tissues were collected for estimation of serum lipid profile, serum liver, and kidney biomarkers. The protection was measured by detecting lipid peroxidation (LPO), glutathione (GSH), superoxide dismutase (SOD), Catalase (CAT) (in liver tissues), and liver histological examination. RESULTS: The results showed a significant (P < 0.05) decrease in levels of serum cholesterol, triglycerides, low density lipoprotein (LDL-C), and very low density lipoprotein (VLDL-C), with a significant increase in level of high density lipoprotein (HDL-C) for RGDS groups compared to induced control. Rats administered a diet containing RGDS levels produced significant (P < 0.05) hepatoprotection by decreasing the activities of liver enzymes, kidney parameters, and lipid peroxidation, while levels of GSH, SOD, and CAT were increased significantly to near the normal levels. CONCLUSION: The RGDS 20% group was more effective than others against hepatotoxicity of paracetamol, which may be attributed to RGDS total phenols and antioxidant contents, which were 1.438 mg and 1.231 mg, respectively.

• Korean Journal of Organic Agriculture
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• v.24 no.3
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• pp.485-495
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• 2016

The aim of the present study was to determine the effect of dietary cultured wild ginseng root (CWGR) supplementation on goat milk composition and ginsenoside profiles. Sixteen Saanen dairy goats were allocated to two balanced groups based on lactation period, body weight ($38.6{\pm}3.2kg$), and dairy milk yield ($2.85{\pm}1.2kg$), and were kept in separate pens. Goats were fed a total mixed ration (TMR) feed (2.3 kg/d, dry matter basis) and 1.5 g of CWGR powder was supplemented in the experimental diet. The total feeding period was 3 weeks, and milk and blood samples were collected on the last three days of the experimental period. There was no effect of CWGR on daily milk yield and milk composition (fat, protein, lactose, and solid-not-fat). However, the CWGR-treatment group had significantly higher plasma IgG and protein contents than the control group (P < 0.05). Significant amounts of ginsenosides were observed in the milk of the CWGR-treatment group, whereas ginsenosides were not detected in the milk of the control group. In conclusion, dietary CWGR was a useful regimen to produce functional goat milk enriched in ginsenosides.

• Journal of Environmental Science International
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• v.27 no.8
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• pp.689-699
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• 2018

The changes on community structures of benthic macroinvertebrates, relevance to the environment and interrelationship between benthos were studied over two years in stream with large environmental disturbance, which caused by localized heavy rain during Typhoon Chaba in October 2016. As a result, the number of species and individuals were increased after localized heavy rain, especially numbers of individuals of Ephemeroptera and Plecoptera were greatly increased. On the contrary, those of Semisulcospira libertina and Semisulcospira forticosta of Mesogastropoda were greatly decreased. Dominant species was Baetis fuscatus of Ephemeroptera, numbers of species and individuals of Ephemeroptera, Plecoptera and Trichoptera(EPT group) were dramatically increased from 26 species, 110 individuals to 32 species, 365 individuals respectively. This suggests that the change of river bed and flow velocity due to heavy rain provided a suitable environment for the EPT group that preferred the rift of a stream. In the functional feeding group, only gathering collectors and filtering collectors were identified in autumn of 2017 because some functional groups preferentially adapted to the changed environment. The interspecific competition and environmental condition were the worst in autumn after heavy rain due to the increase individuals of some species. The ecological score of benthic macroinvertebrate community(ESB) was higher after the heavy rain than before. Results of the Group Pollution Index(GPI), Korean Saprobic Index(KSI) and Benthic Macroinvertebrate Index(BMI) were similar to those before and after heavy rainfall. Therefore, ESB was the most discriminating method for estimating the biological water quality in this study. Some species that are sensitive to water quality changes still appear or increase individuals in the area under investigation after the heavy rain. On the other hand, the individuals of some pollutant species decreased. This is thought to be because the habitat fluctuation caused by heavy rainfall has improved the water environment.

• Journal of Environmental Impact Assessment
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• v.26 no.6
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• pp.404-417
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• 2017

In this study, we analyzed response patterns of rotifer community to eutrophic state, and estimated the applicability of rotifer community as an environmental indicator for highly eutrophicated reservoirs. In order to evaluate the relationships among spatial and temporal distributions and the water quality of rotifer community, we selected the Jundae Reservoir and Chodae Reservoir in Chungcheongnam-do, Korea, which are geographically adjacent but have different water quality, particularly in their eutrophic states. For the analyses on their correlations, monthly survey of water quality and rotifer community, was conducted from April to November 2013 in both reservoirs. The rotifer community was divided into different compositions of functional groups as well as species. Functional groups were classified according to the structure and shape of trophi which can represent feeding behavior of rotifer genus. To reflect ecological characteristics of species, body size and habitat preferences were also considered. Species-based composition did not show a consistent tendency with water quality parameters related with eutrophication. On the contrary, functional group composition showed relatively clear group-specific patterns, increasing or decreasing according to the parameters. The results suggest the possible application of rotifer functional group composition as an indicatorforthe lentic systems, especially hyper-eutrophicated reservoirs. The present study can suggest the applicability based on the field observations from the limited time scale and sites, and further studies on feeding behavior of the rotifer functional group and its interactions with environmental variables are necessary for the further application.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.9
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• pp.1537-1543
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• 2004

The purpose of this study was to determine the effect of dietary linseed oil and canola oil on the deposition n-3 polyunsaturated fatty acid in pork. Twelve pigs weighing 50 kg were offered one of four diets based on corn and soybean meal and containing tallow, linseed oil, canola oil or mixed oil (linseed oil plus canola oil). The pigs were slaughtered at approximately 110 kg of their market live weight. Linseed oil, canola oil or mixed oil did not affect any of the three indicators of growth performance-body weight gain, feed intake and feed efficiency. Saturated fatty acid content of plasma was the highest in tallow oil group, while the plasma proportion of saturated fatty acid was lowered in linseed oil, canola oil and mixed oil group from 11.84% to 16.54% than tallow group (p<0.05). The plasma n-3 polyunsaturated fatty acid was not detected at all in the tallow-fed pigs, while the plasma proportion of n-3 polyunsaturated fatty acid were higher in linseed oil, canola oil and mixed oil from 4.68% to 12.83% than tallow group (p<0.05). All three lipid supplements containing n-3 poly-unsaturated fatty acid increased the content of pork belly $\alpha$-linolenic acid (18:3n-3) by 9.43% relative to the tallow values (p<0.05). Feeding linseed oil or canola oil increased the n-3 : n-6 ratio in pork belly to 0.68, and increased the polyunsaturated fatty acid : saturated fatty acid ratio to 0.70 (p<0.05). This result showed that feeding linseed oil and canola oil can produce novel functional pork enriched in n-3 polyunsaturated fatty acid.

• Korean Journal of Veterinary Service
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• v.44 no.3
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• pp.141-148
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• 2021

The objective of this research was to evaluate the immune response to Salmonella Gallinarum experimentally infected layers fed with Guanosine 5'-monophosphate-chelated calcium and iron feed additives. Hy-Line brown, 34 week-olds layers were assigned to 3 groups; Group 1: basal diet feed, Group 2 (CaFe-GMP): basal diet feed mixed with chelated calcium and iron, and Group 3 (Fe-OCHT): basal diet feed mixed with chitosan for 4 weeks. There were challenged with 1.0×10⁸ CFU/mL of the cultured Salmonella Gallinarum (SG) by oral administration on 28th feeding days. After SG challenge, Flow cytometric profiles showed that the CD4+/CD8+ T lymphocyte activation of Group 2 was much higher than Group 1 and Group 3 (P<0.05). In addition, the levels of interleukin-2 (13.37 mg/dl) and interferon-γ (2.35 mg/dl) in Group 2 were higher than Group 1 and Group 2. Populations of Lactic acid bacteria (3.5×10¹⁰ CFU/g) from cecum was highest observed in group 2. Re-isolation of SG from cecum in group 2 (8×10⁵ CFU/g) was lower than group 1 (1.83×10¹⁰ CFU/g). The result of this study demonstrated that CaFe-GMP feed additive may be one of the potential candidates to control salmonellosis and functional feeds in layers.

• Korean Journal of Ecology and Environment
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• v.36 no.1 s.102
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• pp.21-28
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• 2003

Community composition and functional feeding groups of aquatic insects according to stream order were investigated from the Gapyeong Creek, a typical mid-sized Korean stream in Gyeonggi -do, Korea, in April 2000. For field investigations, the main watercourse and three major tributaries of the stream that belong to stream order ll to Vll were divided into reaches (ca. $1{\sim}4$ km in distance). Aquatic insects were sampled from one or two sites each reach (total 30 sites) using a Surber sampler ($50{\times}50$cm, mesh 0.75 mm). As a result of the quantitative samplings(two Surber samplings at riffle and pool/run per site; total $2{\times}30$=60 Surber samplings) and additional qualitative samplings, a total of 164 species of aquatic insects in 103 genera, 54 families, and 8 orders were collected from the stream. Those aquatic insects were composed of Trichoptera (56spp.: 34.1%), Ephemeroptera (43 spp.: 26.2%), Diptera (25spp.: 15.2%), Plecoptera (23 spp.: 14.0%), Coleoptera (6 spp.: 3.7%), Odonata (6 spp.:3.7%), Hemiptera (3 spp.: 1.8%), and Megaloptera (2 spp.: 1.2%); EPT-group (122 spp.:74.4%) or EPT-group plus Diptera (147 spp.: 89.6%) occupied most of the aquatic insect community; relatively larger number of species occurred in the mid-stream reaches (order III-Vl). The quantitative samplings throughout the study sites yielded a total of 26,286 individuals of aquatic insects ($136{\sim}2522$ inds./0.5 $m^2$, mean 906.4inds./0.5 $m^2$) that belongs to Ephemeroptera (11,994 inds.: 45.6%), Diptera (8730 inds.:33.2%), Trichoptera (4123 inds.: 15.7%), Plecoptera (1213 inds.: 4.6%), Coleoptera (204 inds.: 0.8%), Odonata (13 inds.: 0.05%), Megaloptera (5 inds.: 0.02%), and Hemiptera (4inds.: inds.: 0.02%); average number of individuals of aquatic insects increased as the stream order increased: average numbers of individuals of Bllecoptera and Trichoptera decreased and increased, respectively, as the stream order increased. Tolerant species such as Chironomidae spp., Uracanthella rufa and Hydropsychidae spp. were particularly abundant in the down stream reaches (order Vll) . Species diversity indices (H`) and dominance indices (Dl) were relatively higher and lower, respectively, in the mid-stream reaches (order $IV{\sim}VI$). Shredders occupied the smallest partand collector-gatherers were most abundant among the functional feeding groups(FFGs); collector-filterers considerably increased in the down stream reaches (orders Vl and VII); scrappers were relatively evenly distributed throughout the stream reaches: predators were relatively more abundant in the uppermost stream reaches(order ll) . Overall, the characteristics of aquatic insect comminity and FFGs in the Gapyeong Creek are largely similar to those in the normal streams of temperate deciduous forest in the northern hemisphere that is explained by the river confineum concept.