• Title/Summary/Keyword: Function spaces

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An Analysis of Wooden Wells from the Three Kingdoms Period in the Yeongsan River Basin (영산강유역 삼국시대 목조우물에 대한 검토)

  • CHOI Misook
    • Korean Journal of Heritage: History & Science
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    • v.57 no.3
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    • pp.6-22
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    • 2024
  • This paper examines the characteristics of wooden wells from the Three Kingdoms period that were discovered in the Yeongsan River basin, in addition to their functions based on the distribution of the remains and excavated artifacts found near the wells. A total of 11 wooden wells have been found at six archeological sites along the middle and upper reaches of the Yeongsan River basin. These wooden wells were built in a wider variety of forms than wells made of other materials due to the ease of processing resulting from the physical properties of wood. However, due to the limited geological conditions in which these wells can be installed and their rapid decay, the discovery of such wells is rare. They tend to be located in the clay and mud layers of old river channels or near river channels where it was relatively easy to obtain water from the riverbed. The wooden wells are mostly square or rectangular in shape and were assembled transversely, and some include support beams in their construction. The backfill was reinforced with either stone, a mixture of stone and clay, or a mixture of clay and pottery shards. The material mainly used was pine wood boards, with wood from chestnut trees being used as a sub-material. Various artifacts, such as pottery and wooden containers, animal bones, and seeds, have been excavated in small quantities. The excavated pottery items consist mostly of flat cups with a cover, mounted cups, pottery stands, wide-mouthed jars with a hole, and round pottery. Based on the environment and remains of the sites, the wells are thought to have been used for domestic and production purposes. The assumed primary function was to obtain domestic water, as most of the wells were located within residential spaces where the area's inhabitants lived. The wells were also used to obtain water for agricultural purposes, as well as for productive purposes such as for operating kilns and smelters. Lastly, the wooden wells were also found to be strongly associated with rites, as evidenced by the artifacts found inside them.

Cellular activities of osteoblast-like cells on alkali-treated titanium surface (알칼리 처리된 타이타늄 표면에 대한 골아 유사세포의 세포 활성도)

  • Park, Jin-Woo;Lee, Deog-Hye;Yeo, Shin-Il;Park, Kwang-Bum;Choi, Seok-Kyu;Suh, Jo-Young
    • Journal of Periodontal and Implant Science
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    • v.37 no.sup2
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    • pp.427-445
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    • 2007
  • To improve osseointegration at the boneto-implant interface, several studies have been carried out to modify titanium surface. Variations in surface texture or microtopography may affect the cellular response to an implant. Osteoblast-like cells attach more readily to a rougher titanium surface, and synthesis of extracellular matrix and subsequent mineralization were found to be enhanced on rough or porous coated titanium. However, regarding the effect of roughened surface by physical and mechanical methods, most studies carried out on the reactions of cells to micrometric topography, little work has been performed on the reaction of cells to nanotopography. The purpose of this study was to examme the response of osteoblast-like cell cultured on blasted surfaces and alkali treated surfaces, and to evaluate the influence of surface texture or submicro-scaled surface topography on the cell attachment, cell proliferation and the gene expression of osteoblastic phenotype using ROS 17/2.8 cell lines. In scanning electron micrographs, the blasted, alkali treated and machined surfaces demonstrated microscopic differences in the surface topography. The specimens of alkali treatment had a submicro-scaled porous sur-face with pore size about 200 nm. The blasted surfaces showed irregularities in morphology with small(<10 ${\mu}m$) depression and indentation among flatter-appearing areas of various sizes. Based on profilometry, the blasted surfaces was significantly rougher than the machined and the alkali treated surfaces (p$TiO_2$) were observed on alkali treated surfaces, whereas not observed on machined and blasted surfaces. The attachment morphology of cells according to time was observed by the scanning electron microscope. After 1 hour incubation, the cells were in the process of adhesion and spreading on the prepared surfaces. After 3 hours, the cells on all prepared surfaces were further spreaded and flattened, however on the blasted and alkali treated surfaces, the cells exhibited slightly irregular shapes and some gaps or spaces were seen. After 24 hours incubation, most cells of the all groups had a flattened and polygonal shape, but the cells were more spreaded on the machined surfaces than the blasted and alkali treated surfaces. The MTT assay indicated the increase on machined, alkali treated and blasted surfaces according to time, and the alkali treated and blasted surfaces showed significantly increased in optical density comparing with machined surfaces at 1 day (p<0.01). Gene expression study showed that mRNA expression level of ${\alpha}\;1(I)$ collagen, alkaline phosphatase and osteopontin of the osteoblast-like cells showed a tendency to be higher on blasted and alkali treated surfaces than on the machined surfaces, although no siginificant difference in the mRNA expression level of ${\alpha}\;1(I)$ collagen, alkaline phosphatase and osteopontin was observed among all groups. In conclusion, we suggest that submicroscaled surfaces on osteoblast-like cell response do not over-ride the one of the surface with micro-scaled topography produced by blasting method, although the microscaled and submicro-scaled surfaces can accelerate osteogenic cell attachment and function compared with the machined surfaces.