• Journal of the Korean association of regional geographers
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• v.8 no.1
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• pp.20-39
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• 2002

The purpose of this study is to analyze the characteristics of a life cycle of a theme park through looking at Everland in Yongin, Korea. The life cycle of Everland is divided into four stages. In "initial development" stage(1976 - the mid-80's), Everland become inclined the characteristics of an amusement park by constructing the amusement facilities in the Flower Center(botanical garden). In "growth" stage(the mid-80's - the early 90's), Everland had full grown to the amusement park and inclined to a theme park. In "maturity" stage(the early 90's - the mid-90's), many visitors were not motivated to re-visit Everland because the facilities and events did not change often. Therefore, Everland makes plans for multiple theme park. In "rejuvenation" stage(after the mid-90's), Everland has practiced the plans for multiple theme park and has become the family and accommodating-oriented theme resort park. Since 1976 when Everland was opened, it has evolved from an amusement park to a theme park by creating various and continuous innovations in facilities and events.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.2
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• pp.220-225
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• 1986

This study was conducted to know the effects of temperature and gibberellic acid on the dormancy block-ing of ginseng seeds at different embryo growth stage. Optimum temperature for embryo growth appeared to be I soc at the beginning stage of post ripening (up to dehiscent time), 1$0^{\circ}C$ at middle stage (for 30 days after dehiscence) and 5$^{\circ}C$ at last stage (between 30 and 92 days after dehiscence). And optimum temperature for dehiscence is about 17$^{\circ}C$, also the optimum temperature for dehiscence is higher than that of embryo growth. Germination of ginseng seed with full grown embryo was accelerated at high temperature (25-3$0^{\circ}C$). Germina-tion percentage was 80% at 105th date after dehiscence under 5$^{\circ}C$, 28% at 147th date under 1$0^{\circ}C$, but no germination under the over 15$^{\circ}C$. Gibberellic acid increased the dehiscent rate, whereas the gibberellic acid treatment may not be substituted for effect of low temperature on the germination. Low temperature may be reguired to finish the embryo growth in thickness.

• The Korean Journal of Malacology
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• v.27 no.4
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• pp.359-369
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• 2011

Twelve species of food microalgae were investigated to clarify the digestion index of Crassostrea gigas larvae using epifluorescence microscopy to choose an appropriate diet for artificial seed production in hatchery. An experiment was conducted using 1 (D shaped stage), 4 (Early umbo stage), 8 (umbo stage) and 12 (Full grown stage) days old larvae. larvae were stocked in 1 L flasks at 5 individuals/mL and fed $10{\times}10^4$ algal cells/mL of each species individually. Prior to larvae were fed for 3 h and then were observed under the microscope to detect ingestion; larvae were then sieved and replaced in 1 L flasks containing filtered seawater and were observed after 3, 5 and 8 h to analyse the digestion index. Values of digestion indices were specific for each alga. No evidence for the ingestion of Thalassiosira weissflogii was evident at all larval development stages tested. Digestion indices of others microalgae were 0.8-99.7% at 4 stage of larval development stages: Chlorella ellipsoidea (0.8-5.4%), Nannochloris oculata (1.4-5.0%), Isochrysis galbana (99.1-99.5%), Pavlova lutheri (99.1-99.5%), I. aff. galbana (99.4-99.5%), Cheatoceros calcitrans (0.0-99.2%), C. gracilis (0.0-99.7%), C. simplex (0.0-95.9%), Phaeodactylum tricornutum (0.0-99.6%), Tetraselmis tetrathele (0.0-99.7%) and Dunaliella tertiolecta (0.0-99.6%), respectively. Therefore, it is assumed that food microalgae showing the high digestion such as I. galbana should be supplied to the early umbo stage larvae, and then after the umbo larval stage, the mixed microalgae with diatoms and light green algae should be supplied to the full grown stage larvae to increase the digestion of their larvae.

• Journal of The Korean Society of Grassland and Forage Science
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• v.6 no.1
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• pp.31-37
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• 1986

In order to study the possibility of grassland improvement by spring sowing in the forest, microenvironmental conditions, emergence, percentage of grasses and weeds, root weight and dry matter yield of grasses were investigated. Two field sites (forest grassland and full-sunlight grassland) and two sowing times (March 20 and April 10) were assigned. The condition of the forest grassland was area of pine trees with 50% shading, and the experiment was performed at the Livestock Experiment Station in Suweon, 1984. The results obtained are summarized as follows: 1. For germination and early growth of grasses, full-sunlight grassland was more advantageous than forest grassland. Growth after that stage, on the other hand, forest grassland was more suitable. Especially, during dry and high temperature season, temperature of soil surface and underground in the forest grassland were decreased by $6-7^{\circ}C$ and $3-4^{\circ}C$ each other, compared with those of the full-sunlight grassland. Also soil moisture content was continuously higher in the forest grassland. 2. At March 20 sowing the emergence time in the full-sunlight grassland was shortened by 8 days, compared with that of the forest grassland. In case of sowing on April 10, however, there was no difference between two grassland sites. 3. Grasses grown in the forest was more prostrate and leaves from them decayed more, compared with those of the full-sunlight grassland. 4. The percentage of grasses in the forest grassland was 80 to 85 %, on the other hand, that of the full-sunlight grassland was only 15 to 20 %. And the percentage of grasses tended to be high in the plot of early sowing time. 5. Dry root weight and root length of grasses grown in the forest were inferior to those of the full-sunlight grassland (P<0.05), but there was no significant difference between two sowing times. 6. Dry matter yield of grasses was significantly higher (P<0.05) in the forest grassland than in the full-sunlight grassland, and yield was influenced by sowing time. Higher yield (4,011 kg/ha) was produced in the plot of the forest grassland with early spring sowing. 7. From above results, it is suggested that grassland improvement by spring sowing in the forest is possible, and it is desirable to sow in early spring.

• Proceedings of the KSAR Conference
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• 2000.10a
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• pp.9-9
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• 2000

The mammalian ovary has a large number of primordial and preantral follicles, which are a potential source of oocytes for the in vitro mass production of embryos. Several in vitro culture systems have been developed to support the growth and development of oocytes from mouse preantral follicles. Under the appropriate condition, meiotically incompetent oocytes from preantral follicles can grow to final size and complete nuclear maturation in vitro. Furthermore, the successful production of live young from in vitro grown and matured oocytes demonstrates that oocytes from preantral follicles are able to acquire full developmental capacity in vitro. However, the efficiency of in vitro production of embryos from mouse preantral follicles is still low. In farm animals as well as human, the growth of oocyte from preantral follicle to the meiotic competence stage has yet to be demonstrate. Therefore, further studies to improve the culture condition or to develope new culture system should be needed in the future. In addition, the visible progress in the establishment of the in vitro culture system for preantral follicles of farm animals and human could help to enlarge the populations of valuable agricultural, phamaceutical product-producing, and endangered animals, and to rescue the oocytes of women about to undergo clinical procedures that jeopardize oocytes.

• Journal of Sericultural and Entomological Science
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• v.9
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• pp.43-47
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• 1969

This experiment was carried out to investigate the effect of gamma ray on the eggs of later stage in late fall rearing season. The results are summarized as follows: 1. The duration of larval stage was simmilar to that of control in any dose. The silkworm growth was uniform in does of 4,000 gamma ray, but in does of 8,000r 16000r was irregular. There were not malformed silkworms but many small silkworm. 2. Mortality of silkworms was very high 8,000r and over; virus diseases of 87.6 per cent, flacherie of 5.9 per cent and complication of 6.5 per cent. 3. The more the dose of gamma ray became, the lighter the weight full grown silkworm and matured silkworm became. 4. The uniformness of cocoons was registered in dose of 2,000r and the cocoons were irregular or small in dose of 16,000r

• Korean Journal of Ichthyology
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• v.13 no.4
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• pp.254-260
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• 2001

In the goby Micropercops swinhonis, the follicular layer of full-grown oocytes consists of an outer layer (theca cell) and an inner layer (granulosa cell). As the oocyte grows, columnar cells of inner granulosa layer secrete mucin to their cytoplasm and then surround the oocyte. Such granulosa cells appear to be cuboidal cells in the early vitellogenesis, yolk vesicle stage, to be replaced by columnar cell secreting mucins (adhesive materials) in the middle vitellogenesis, yolk granule stage. The enveloping layer of the oocyte has a muco- follicle layer filled with mucins. The mucins are an amorphous and electron-dense substance. Interestingly, the oocyte enveloping layer becomes thickened towards the animal pole as vitelogenesis proceeds. A zona radiata of about $7.8{\sim}11.5\;{\mu}m$ thick is present below the muco-follicle layer. The zona radiata is composed of an one-layered electron-dense externa and a three to five-layered electron-less interna.

• Parasites, Hosts and Diseases
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• v.27 no.4
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• pp.231-248
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• 1989

The growth and development of the metacercariae of F. seoulensis cultivated in vitro or on the chick chorioallantois were assessed by comparison with the optimum process of maturation in albino rats and new born chickens. The process of maturation was divided for convenience into six stages: Stage 1 ; cell multiplication, Stage 2; body shaping, Stage 3; separation of genital anlagen, Stage :1 organogeny, Stage 5; gametogony, and Stage 6: oviposition. In Hank's and Tyrode's .solutions, the metacercariae were alive up to 200 days or more at $4^{\circ}C$ without any development. The in vivo maturation process in rats or chicks was as follows: stage 1 from 6 hours; stage 2 from 24 hours; stage 3 from 48 to 72 hours; stage 4 from 3 to 4 days; stage 5 from 4 to 5 days; and stage 6 from 5 to 8 days. Despite unsuccessful infection of the metacercariae to 12 day old chicks, fully mature worms of stage 5 or 6 were recovered from new born chicks (1 to 2 days old), The metacercariae of F. seoulensis grown in vitro were up to stage 3 and no further maturation was observed. Of various media employed, the medium NCTC 109 (Gibco) or NCTC 135(Gibco) supplemented with 20% egg yolk or 20% whole egg macerate or 0.5% yeast was basically required for the earlier development of the fluke. It took 16.1 days(in average) to reach the stage 3 after cultivation. The metacercariae cultivated on the chorioallantoic membranes of 6∼13 day old chick embryo at 37∼38℃ showed their full development up to stage 5 or 6. However, the worms were in general remarkably retarded, compared with those grown in rats or chickens. In the experiments of worm transplant, although the transfer was failed from in vitro culture to in vivo of rats(Per os), the transplants from in vitro culture to the chorioallantois and from the choriollantois to in vivo of rat host were successful with or without development of the transferred worms. In the present study, it was observed that the metacercariae of F, seoulensis can be maintained in vitro media with poor development as well as fully matured in 1 to 2 day-old chicks or on the chorioallantois at a very low rate.

• Korean Journal of Environmental Biology
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• v.33 no.4
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• pp.419-424
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• 2015

In order to determine the ovarian cycle of Asian Toad, Bufo gargarizans, the developmental stage based on the gonadosomatic index (GSI), size of follicle oocytes in ovary and vitellogenesis for adult females were investigated all around the year. The weight of ovary and GSI were the lowest from April, and all follicle oocytes exist in the pre-vitellogenic form, indicating that the vitellogenesis was suspended. The follicle oocytes in early-vitellogenic stage appeared in ovary during may when the weight of ovary and GSI start to increased, and the follicle oocytes in mid-vitellogenic and pre-vitellogenic stages existed during June and the weight of ovary and GSI also increased. This indicates that vitellogenesis has been carried out actively during this period. The follicle oocytes in mid-vitellogenic stage and late-vitellogenic stage when the vitellogenesis was also completed existed on September. Post-vitellogenic follicle oocytes after vitellogenesis started to appear from October and rapidly increased from December in hibernation. The full grown follicle oocytes existed during February, indicating the ovarian cycle that all follicle oocytes in ovary are developed separately, not synchronized, during the growing period of follicle oocytes and the post-vitellogenic follicle oocytes are maintained the ovulation period.

• Korean Journal of Environment and Ecology
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• v.28 no.3
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• pp.287-292
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• 2014

In order to determine the ovarian cycle of Korean brown frog, Rana coreana, the developmental stage based on the gonadosomatic index (GSI), size of follicle oocytes in ovary and vitellogenesis for adult females were investigated all around the year. The weight of ovary and GSI were the lowest from March to May, and all follicle oocytes existed in the pre-vitellogenic form, indicating that the vitellogenesis was suspended. The follicle oocytes in early-vitellogenic stage appeared in ovary during June when the weight of ovary and GSI started to increase, and the follicle oocytes in mid-vitellogenic and pre-vitellogenic stages existed during August and the weight of ovary and GSI also increased. This indicates that vitellogenesis has been carried out actively during this period. The follicle oocytes in mid-vitellogenic stage and late-vitellogenic stage when the vitellogenesis was also completed existed between September and November. Post-vitellogenic follicle oocytes after vitellogenesis started to appear from December in hibernation, and the full grown follicle oocytes existed during February, indicating the ovarian cycle that all follicle oocytes in ovary are developed separately, not synchronized, during the growing period of follicle oocytes and the post-vitellogenic follicle oocytes are maintained the ovulation period.