• KSBB Journal
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• v.23 no.5
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• pp.445-448
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• 2008

Candida antarctica lipase B (CalB) is an efficient biocatalyst for many organic synthesis reactions. To make full use of CalB, we need effective expression system. Previously recombinant CalB was successfully expressed in the methylotropic yeast Pichia pastoris. In addition, we succeed in the functional expression of CalB in the Escherichia coli cytoplasm. This CalB expression system in E.coli has many considerable advantages in comparison with other expression systems and enables high-throughput screening of gene libraries as those derived from directed evolution experiments. To optimize E.coli system, we investigate comparing between OrigamiB (DE3) and BL21 (DE3) and observing effect of IPTG amount.

• Bulletin of the Korean Chemical Society
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• v.28 no.12
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• pp.2303-2309
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• 2007

Expressed protein ligation (EPL) technique, joining recombinantly expressed proteins to polypeptides, has been widely adopted for addressing various biological questions and for drug discovery. However, joining two recombinant proteins together is sometimes difficult when proteins are expressed insoluble and unrefoldable, because ligation-active proteins via intein-fusion are obtainable when they are folded correctly. We overcame this limitation coexpressing target protein with additional methionine aminopeptidase (MAP) which enhances removal of the initiation methionine of recombinantly expressed protein. Our approach demonstrated that two domains of 46 kDa 5-Enolpyruvylshikimate-3-phosphate (EPSP) synthase, a target of herbicide glyphosate, were successfully joined by native chemical ligation, although its C-terminal domain was expressed as an inclusion body. The intein-fused N-terminal fragment of EPSP synthase (EPSPSN, residues 1-237) was expressed and the ligation-active thioester tagged N-terminal fragment (EPSPSN-thioester) was purified using a chitin affinity chromatography and mercapto-ethanesulphonate (MESNA) as intein thiolysis reagent. Its Cterminal fragment (EPSPSC, residues Met237-238CYS-427), expressed as an inclusion body, was prepared from an additional MAP-expressing strain. Protein ligation was initiated by mixing ~1 mM of EPSPSN-thioester with ~2 mM of EPSPSCCYS (residues 238CYS-427). Also we found that addition of 2% thiophenol increased the ligation efficiency via thiol exchange. The ligation efficiency was ~85%. The ligated full-length EPSP synthase was dissolved in 6 M GdHCl and refolded. Circular dichroism (CD) and enzyme activity assay of the purified protein showed that the ligated enzyme has distinct secondary structure and ~115% specific activity compared to those of wild-type EPSP synthase. This work demonstrates rare example of EPL between two recombinantly expressed proteins and also provides hands-on protein engineering protocol for large proteins.

• Clinics in Shoulder and Elbow
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• v.23 no.2
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• pp.86-93
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• 2020

Background: Several systematic reviews have reported on the conservative treatment of full-thickness rotator cuff tears; however, clinical results of this treatment still remain determined. Methods: PubMed, Cochrane Library, PEDro, and CINAHL databases were systematically searched for randomized clinical trials and observational studies. Two independent researchers reviewed a total of 2,981 articles, 28 of which met the criteria for inclusion in the study. Clinical outcome measures included Constant score, visual analog scale score for pain, range of motion, and short-form 36 questionnaires. The meta-analysis used a linear mixed model weighted with the variance of the estimate. Results: The meta-analysis showed a significant improvement after surgery. Pain score is 26.2 mm (1 month) to 26.4 mm (3 months), and 24.8 mm (12 months) (P<0.05); active abduction: 153.2° (2 months), 159.0° (6 months), 168.1° (12 months) (P<0.05); Constant score: 67.8 points (2 months) to 77.2 points (12 months) (P<0.05); short-form 36 questionnaires "vitality" section: 57.0 points (6 months) to 70.0 points (12 months) (P<0.05). Conclusions: Our data confirmed the effectiveness of conservative treatment in patients with full-thickness rotator cuff tears 12 months post-intervention. The results suggest that conservative treatment for patients with full-thickness rotator cuff tears should be the first line of treatment before considering surgery.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.5
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• pp.645-652
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• 2000

A total of 80 piglets ($5.18{\pm}0.61kg$ of BW; 16 d of age) were fed experimental diets to evaluate the effect of extruded full-fat soybean (EFS) on the growth of eatly weaned pigs. Pigs were allotted into five treatments based on body weight, in a completely randomized block design. Each treatment has 4 replicates of 4 animals each. Treatments consisted of diets representing substitutional ratios of EFS for soybean meal. 1) 100:0 (SBM), 2) 75:25 (EFS 25), 3) 50:50 (EFS 50), 4) 25:75 (EFS 75) and 5) 0:100 (EFS 100). During phase I (d 0 to 7), piglets were fed diets containing 3,340 kcal ME, 26% crude protein, 1.85% lysine, 1.0% Ca and 0.9% P. For phase II (d 8 to 21), piglets were fed diets supplying 3,340 kcal ME, 23% crude protein, 1.65% lysine, 0.9% Ca and 0.8% P. Urease activity (pH rise) of EFS (0.18) was three times higher than that of SBM (0.06) indicating that processing conditions were not efficient enough to inactivate urease activity. During the first week postweaning, pigs fed SBM had significantly greater average daily gain (ADG), average daily feed intake (ADFI) and better feed conversion ratio (FCR) compared to pigs fed FFS diets. Linear negative effect on growth rate was found as the inclusion rate of FFS increased. During d 8 - 21 postweaning, piglets fed EFS 50 diet showed the best ADG and FCR despite no significant difference between treatment SBM and EFS 25 have been observed. Overall, piglets fed diets up to 50% FFS inclusion rate exhibited similar weight gain. Only piglets fed EFS 100 diet showed a significantly decreased growth rate. No other significant effect was found in feed intake and feed conversion ratio. At d 7, dry matter digestibility was higher in pigs fed SBM diet than piglets fed EFS 75 diet (p<0.05) and crude protein digestibility was higher in piglets fed SBM diet than piglets fed EFS 50, EFS 75 and EFS 100 (p<0.05). At d 21, no difference other than in phosphorus digestibility was detected. This indicates that piglets at 21 d postweaning are capable of utilizing nutrients from FFS. No treatment effects were detected in blood metabolites. The data suggests that piglets at 16 d of age are not sufficiently mature to use extruded FFS in their diets. Nevertheless, FFS seemed to be able to replace upto 50% of SBM in weaned piglet diet.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.11 no.5
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• pp.218-223
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• 2001

The chemical-mechanical polishing process was carried out for 2"-dia. sapphire wafer grown by horizontalBridgman method on the urethane lapping pad with the silica sol. The polished wafer shows the full-width at halfmaximum of 200~400 arcsec in double-crystal X-ray diffraction, indicating that the slicing, grinding and lapping processes before the polishing process affected the crystalline structural property of the wafer surface by the mechanical residual stress. For the inclusion of surface treatments after chemical-mechanical polishing such as the thermal annealing at the temperature of $1,200^{\circ}C$for 4 hrs. and chemical etching, the crystalline quality was sigdicantly enhanced with the reduced full-width at half maximum up to 8.3 arcsec.arcsec.

• Journal of the Korean Society of Combustion
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• v.15 no.3
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• pp.32-39
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• 2010

An experimental study was conducted to investigate the relationship between global equivalence ratio(GER) and fire characteristics in an ISO 9705 room. Heptane fuel was burned with different fuel flow rates and doorway widths in order to force the room to be placed in different GER conditions. It was observed that after the onset of under-ventilated fire conditions, temperature and unburned fuel components such as CO and soot increased with increasing heat release rate (HRR), regardless of the doorway width. From the analysis of local mixture fraction, it was reconfirmed that the inclusion of soot production in the product composition was very important to predict accurately the chemical conditions inside the compartment, particularly for the under-ventilated fire conditions. In addition, the local equivalence ratio (LER) was directly proportional to the GER with a unit gradient regardless of doorway width when the soot production was included in the chemical products. This finding provided an important potential that the GER could be used to correlate the local thermal and chemical environment measured at the upper layer of a full-scale enclosure when soot was included.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.6
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• pp.865-871
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• 2017

Objective: The aim was to evaluate carcass characteristics, cut yield, and meat quality in lambs that were fed different inclusion levels of palm kernel cake. Methods: Forty-five woolless castrated male Santa $In{\hat{e}}s$ crossbred sheep with an initial average body weight of $23.16{\pm}0.35kg$ were used. The experimental design was a completely randomized design with five treatments, with palm kernel cake in the proportions of 0.0%, 7.5%, 15.0%, 22.5%, and 30.0% with nine replications per treatment. After slaughter, the gastrointestinal tract was weighed when it was full, after which it was then emptied. The heart, liver, kidney, pancreas perirenal fat were also collected and weighed. The carcass was split into two identical longitudinal halves and weighed to determine the quantitative and qualitative characteristics. Results: The empty body weight, carcass weight and yield, and fat thickness decreased linearly (p<0.05) as a function of palm kernel inclusion in the diet. There was no difference (p>0.05) for the rib eye area of animals that were fed palm kernel cake. There was a reduction in the commercial cut weight (p<0.05), except for the neck weight. The weights of the heart, liver, kidney fat, small, and large intestine, and gastrointestinal tract decreased. Nevertheless, the gastrointestinal content was greater for animals that were fed increasing levels of cake. For the other organs and viscera, differences were not verified (p>0.05). The sarcomere length decreased linearly (p<0.05), although an effect of the inclusion of palm kernel cake was not observed in other meat quality variables. It is worth noting that the red staining intensity, indicated as A, had a tendency to decrease (p = 0.050). Conclusion: The inclusion of palm kernel cake up to 30% in the diet does not lead to changes in meat quality characteristics, except for sarcomere length. Nevertheless, carcass quantitative characteristics decrease with the use of palm kernel cake.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.1
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• pp.102-110
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• 2015

The purpose of this experiment was to determine and compare the digestibility of crude protein (CP) and amino acids (AA) in full-oil (no oil extracted) and de-oiled (oil extracted) corn distillers dried grains with solubles (DDGS) with different condensed distillers solubles (CDS) ratios. Six barrows ($29.6{\pm}2.3kg$) fitted with ileal T-cannula were allotted into a $6{\times}6$ Latin square design. Each period was comprised of a 5-d adaption period followed by a 2-d collection of ileal digesta. The five test diets contained 62% DDGS as the sole source of AA. A nitrogen-free diet was used to measure the basal endogenous losses of CP and AA. Chromic oxide (0.3%) was used as an index in each diet. The results showed that CP and AA were very similar in 5 DDGS, but the standardized ileal digestibility (SID) of lysine (from 56.16% to 71.15%) and tryptophan (from 54.90% to 68.38%) had the lowest values and largest variation within the essential AA, which suggests reduced availability of AA and different levels of Maillard reactions in the five DDGS. The apparent ileal digestibility and SID of CP and most of AA in full-oil DDGS (sources 1 and 2) were greater (p<0.05) than de-oiled DDGS (sources 3, 4, and 5). Comparing the AA SID in the 5 DDGS, full-oil with low CDS ratio DDGS (source 1) had non-significantly higher values (p>0.05) than full-oil with high CDS ratio DDGS (source 2); however, the SID of most AA of de-oiled with low CDS ratios DDGS (source 3) were non-significantly lower (p>0.05) than de-oiled with high CDS ratio DDGS (source 4); and the de-oiled DDGS with middle CDS ratio (source 5) but with different drying processing had the lowest SID AA values. In conclusion, de-oiled DDGS had lower SID of CP and AA than full-oil DDGS; a higher CDS ratio tended to decrease the SID of AA in full-oil DDGS but not in de-oiled DDGS; and compared with CDS ratio, processing, especially drying, may have more of an effect on AA digestibility of DDGS.

• Journal of Multimedia Information System
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• v.4 no.1
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• pp.19-26
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• 2017

The compression of video for both full HD and UHD requires the inclusion of extra vertical lines to every video frame, named as the DTV essential hidden area (DEHA), for the effective functioning of the MPEG-2/4/H encoder, stream, and decoder. However, while the encoding/decoding process is dependent on the DEHA, the DEHA is conventionally viewed as a redundancy in terms of channel utilization or storage efficiency. This paper proposes a block mode DEHA method to more effectively utilize the DEHA. Partitioning video block images and then evenly filling the representative DEHA macroblocks with the average DC coefficient of the active video macroblock can minimize the amount of DEHA data entering the compressed video stream. Theoretically, this process results in smaller DEHA data entering the video stream. Experimental testing of the proposed block mode DEHA method revealed a slight improvement in the quality of the active video. Outside of this technological improvement to video quality, the attractiveness of the proposed DEHA method is also heightened by the ease that it can be implemented with existing video encoders.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.6
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• pp.500-504
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• 2005

Fusion ferritin (heavy chain ferritin, $F_H+$ light chain ferritin, $F_L$), an iron-binding protein, was primarily purified from recombinant Escherichia coli by two-step sonications with urea [1]. Unfolded ferritin was refolded by gel filtration chromatography (GFC) with refolding enhancer, where 50 mM Na-phosphate (pH 7.4) buffer containing additives such as Tween 20, PEG, and L-arginine was used. Ferritin is a multimeric protein that contains approximately 20 monomeric units for full activity. Fusion ferritin was expressed in the form of inclusion bodies (IBs). The IBs were initially solubilized in 4 M urea denaturant. The refolding process was then performed by decreasing the urea concentration on the GFC column to form protein multimers. The combination of the buffer-exchange effect of GFC and the refolding enhancers in refolding buffer resulted in an efficient route for producing properly folded fusion ferritin.