• Korean Journal of Microbiology
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• v.37 no.4
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• pp.239-244
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• 2001

We isolated a Myxobacteria strain from a soil sample obtained from Mt. Daedoon located in Choongnam, Korea. This strain, ARJ, secreted slime while swarmed on the surface of CT medium. It produced greenish yellow pigment in liquid or solid media, and the swarming edge showed green florescence under U. V. at 366 nm. It formed fruiting bodies when nutrient was exhausted, which is one of the most imkportant characteristics of Myxobacteria. The fruiting bodies did not have a stalk and consisted of naked myxospores when examined under the scanning electron microscope. These traits lead us to believe that this strain is very close to Myxococcus virescens. It showed antimicrobial activity, especially against Gram positive bacteria. Culture filtrate showed the activity but this was not due to protein. The culture filtrate also had proteolytic activity in which at least two enzymes are involved.

• Journal of Mushroom
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• v.22 no.3
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• pp.112-116
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• 2024

We developed a new oyster mushroom cultivar named 'Dawon-tari', which has a longer stipe and higher yield than those of 'sootari'.'Dawon-tari' was crossed by mating monokaryons isolated from 'Sootari' and 'Daejang2ho'. The optimal temperature for mycelial growth was determined to be 20~25℃ on potato dextrose agar medium, while the optimal temperatures for primordia formation and fruiting body growth were 15~17℃ on a sawdust substrate. During bottle cultivation, the mycelial growth phase required approximately 26 days. Additionally, primordia formation required 5 days, and fruiting body growth took 4 days. The fruiting bodies exhibited a shallow funnel shape; were grayishbrown; and the stipes were characterized by a long, thin structure. The yield of fruiting bodies was 185 g per 1,100 mL bottle, which was 5% higher than that of 'Sootari'.

• Journal of Mushroom
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• v.12 no.2
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• pp.82-87
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• 2014

Commonly known as the button mushroom, Agaricus bisporus is one of the most widely cultivated mushroom species of edible fungi. In the breeding of new button mushroom, Seolwon was developed by crossing two homokaryons. Because of the predominantly pseudohomothallic life cycle, only a small percentage of homokaryotic meiospores are produced, which do not fruit. Homokaryotic cultures derived from these types of single spores produce a vegetative mycelium that contain a variable number of genetically identical nuclei per cell. After crossing two homokaryons, hybrids were cultivated on a small scale and on a commercial scale at a farm. The spawn was made by a commercial spawn producer and the spawned compost by a commercial compost producer. Mycelial growth of Seolwon on CDA was better at $25^{\circ}C$ when it was compared with that of Seolgang. The mature cap shape of new strain Seolwon is oblate spheroid and the immature cap shape is round to oblate spheroid. The cap diameter was 39.7 mm on average. In comparison with white strain Seolgang, the strain had a yield that was 11% higher. It produced fruiting bodies which had a higher weight on average per fruiting body and were 9.7% firmer with a good shelf life. Days of fruiting body were 1-2 days later than those of Seolgang. The physical characteristics such as springiness, chewiness, adhesiveness, gumminess were better than that of Seolgang.

• The Korean Journal of Mycology
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• v.51 no.4
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• pp.389-396
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• 2023

In this study, dikaryotic strains of Lentinula edodes were generated by mono-mono hybridization using mating type analysis and their fruiting body characteristics were investigated. Approximately 100 monokaryotic strains were isolated from the basidiospores of Sanbaekhyhang, and homokaryotic strains were isolated from Chungheung 1ho using protoplast isolation and regeneration. Their mating types were evaluated and a total of 60 dikaryotic strains were hybridized. Using these strains, fruiting bodies were produced and their characteristics were examined after cultivation on sawdust media. The results indicated that the rate of hybridization was 100% and that 55 of 60 strains formed fruiting bodies. These showed normal pileus and gill structures; however,10 strains also produced fruiting bodies with abnormal pileus and gill structures. The weight and size of the fruiting bodies differed depending on the strains. Overall, further studies are needed for predicting the characteristics of hybridized strains based on their parental strains.

• The Korean Journal of Mycology
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• v.41 no.4
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• pp.225-230
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• 2013

We investigated the cultivation characteristics of Neolentinus lepideus (21 strains derived from Korea and Japan) on Pinus densiflora sawdust medium as follows; period of mycelial growth, fruiting period, productivity of fruit body and color and morphology of fruit body. The periods for mycelial growth and fruiting on the sawdust medium of N. lepideus were 40~65 days and 6~22 days, respectively. It was produced approximately 48.3 g of fresh weight and 9 fruit-bodies per 600 g of sawdust medium. The productivity of fruit body was reduced by scratching off the surface of sawdust medium. We suggest that three strains (KFRI 1199, 1302, 1331) might be good candidates for sawdust cultivation.

• Journal of Mushroom
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• v.8 no.2
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• pp.51-58
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• 2010

Total eight different winter mushroom (Flammulina velutipes) strains were characterized by comparisons of fruiting body morphology, hardness and color after growing at different temperature. Although variations depending on the strains were observed, in general, both stipe length and pilus diameter were shorter while pilus thickness was bigger as growing temperatures were decreased. No significant differences in stipe thickness were observed. The ASI4065 and 4151 had the tallest stipe and the ASI4021 had the shortest stipe. The ASI4103 strain had the biggest pilus diameter. The ASI4153 had most thick pilus, while the ASI 4021 had least thick pilus. Also, the ASI4166 had the most thick stipe and the ASI4065 had the most thin stipe. The hardness of white mushrooms were increased by decreasing growth temperatures, while no relationships between the hardness of brown mushrooms. In most strains, stipe were much stronger than pilei and white mushrooms were harder than brown ones. The strain having most strong stipe was ASI4021. Both ASI 4021 and ASI4166 had the most strong pilus. The color of pilus has much lighter than that of stipe. White mushrooms had high brightness value ranging from 70-90, while brown mushrooms showed low brightness value from 30-70. Saturation value was higher in brown mushrooms. Based on our study, it was shown that environmental conditions had some effects on winter mushrooms, but genetic differences among strains were more important factors to determine most characteristics of winter mushrooms.

• The Korean Journal of Mycology
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• v.49 no.3
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• pp.295-302
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• 2021

The effect of punching treatment on bags containing sawdust medium for the stable production of fruiting bodies of Lentinula edodes 'Hwadam' was investigated. After spawn inoculation, the sawdust medium temperature and CO₂ concentration reached 27.0-28.8℃ on the 23^rd day of incubation, and 14.0-15.6% on the 16^th day of incubation, respectively, decreasing thereafter. The O₂ concentration showed an opposite pattern to the CO₂ concentration. As the number of punching treatments on the medium increased during the incubation period, the lightness value decreased, and conversely, the color difference and weight loss rate increased. There was no difference in the cultivation period according to the punching treatment. As the punching treatment increased on the sawdust medium, the fruiting body yield increased; a three-time punching treatment produced the best yield with 605.7 g. Thus, proper punching treatment on a sawdust medium during the incubation period can increase the number of fruiting bodies and yield.

• The Korean Journal of Mycology
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• v.49 no.1
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• pp.57-66
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• 2021

Six strains among the shiitake varieties developed by the National Institute of Forest Science were used to select suitable varieties for sawdust block cultivation method. After 100 days cultivation (60 days in dark and 40 days in light), the weight loss rate of the sawdust block media was 14.4% (12.8% to 15.7%) in average. After 5 to 7 days of fruiting process, 2~36 primordia per medium were formed. The number of primordium formation was in the following order: Manchuhyang> Sansanhyang> Sanbaekhyang> Bambithyang> Sangjanghyang> Taehyanggo. The stipe length was 41~59 mm as followed by Taehyanggo (59 mm), Sanbackhyang (56 mm), Bambithyang (54 mm), Manchuhyang (53 mm), Sanjanghyang (45 mm) and Sansanhyang (41 mm). Among the strains, Sansanhyang had short and thick stipe. The average weight of fruiting bodies was 33~77 g as following order of Taehyanggo (77 g), Bambithyang (51 g), Sansanhyang (50 g), Manchuhyang (41 g), Sanjanghyang (34 g) and Sanbackhyang (33 g). Mushroom production per 2 kg medium was the highest in Sanbackhyang (473 g) followed by Bambithyang (287 g), Manchuhyang (175 g), Sansanhyang (109 g), Sanjanghyang (78 g) and Taehyanggo (60 g). The hardness of the cap was 1,218 to 1,839 g/10 mm. Taehyanggo was the hardest (1,839 g/10 mm) and Bambithyang was the softest (1,218 g/10 mm). In conclusion, in the cultivation condition of sawdust block medium, mushroom production was good in Sanbackhyang and Bambithyang. Sansanhyang showed good in terms of fruiting body shape.

• Journal of Mushroom
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• v.13 no.1
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• pp.74-78
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• 2015

The 'Heuktari', a new mid-high temperature adaptable variety of oyster mushroom for the bottle culture, was bred by mating with monokaryons isolated from 'P11056' and 'MT07156'. The optimum temperature for the mycelial growth was $23{\sim}26^{\circ}C$ on PDA medium and that for the primordia formation and the growth of fruiting body of 'Heuktari' was $18{\sim}19^{\circ}C$ on sawdust substrate. In case of bottle cultivation, the period of mycelial growth was required about 30 days. In addition, the period of primordia formation and growth of fruiting body was 4 days and 5 days, respectively. In the characteristics of fruiting body, shape and color of pilei were round type and dark grayish brown, stipe color was white color and stipe shape was short and thick. The yield of fruiting bodies was 180 g/900 ml bottle which was 15% higher than that of Suhan-1ho. The gumminess and brittleness of stipe tissue were 110% and 140% stronger than those of Suhan-1ho, respectively.

• Journal of Mushroom
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• v.16 no.3
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• pp.180-185
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• 2018

'Sootari', a new variety of oyster mushroom, was bred by mating with monokaryons isolated from 'Suhan' and 'Gonji-7ho'. The optimum temperature for the mycelial growth was $20{\sim}25^{\circ}C$ on PDA medium and those for the primordia formation and growth of fruiting body of 'Sootari' were $16{\sim}18^{\circ}C$ on sawdust substrate. In bottle cultivation, mycelial growth required about 25 days. In addition, primordia formation and growth of fruiting body required 4 days each. Regarding characteristics of the fruiting body, the shape and color of pileus were round type and black, respectively, and stipe color and shape were white and short and thin, respectively. The yield of fruiting bodies was $131.3{\pm}26.0g$ per 1,100 mL bottle, which was 2% higher than that of Gonji-7ho.