• Title/Summary/Keyword: Fruiting

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Optimization for Effective Bioproduction of Natural (-)-1-Octen-3-ol by Lipoxygenase and Hydroperoxide Lyase from Agaricus bisporus (Agaricus bisporus의 Lipoxygenase와 Hydroperoxide Lyase를 이용한 천연 (-)-1-Octen-3-o1 생산 공정의 최적화)

  • Kim, Kyoung-Ju;Kim, Yong-Hwi
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.6
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    • pp.899-903
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    • 2005
  • One of the most important volatile aroma compounds responsible for mushroom flavor is 1-octen-3-ol. To meet the demand for natural mushroom flavor, a study was needed for the production of natural chiral specific (-)-1-octen-3-ol that has higher flavor intensity than synthetic chiral mixtures of (+), and (-)-1-octen-3-ol. The biosynthesis of (-)-1-octen-3-ol was achieved by an aerobic oxidation using lipoxygenase (LOX) and hydroperoxide lyase (HPOL) isolated from commercially available mushrooms in Korean market. Safflower oil from Uiseong, Gyeongsangbuk-do, that contains $\geq75\%$ of linoleic acid, was hydrolyzed using lipase. The recovered linoleic acid was biotransformed to stereo-specific 10-hydroperoxy linoleic acid by LOX. 10- hydroperoxy linoleic acid was further cleaved to (-)-1-octen-3-ol by HPOL. A commercial bioprocess for the production of (-)-1-octen-3-ol was developed using a 5-liter jar fermenter with fruiting bodies of Agaricus bisporus harvested from Buyeo, Chungcheongnam-do. The maximum production of (-)-1-octen-3-o1 was achieved at $4^{\circ}C$, pH 6.5 and 800 rpm yielding 748 mg/kg of mushroom.

Pretreatment and Storage Condition of Abnormal Fermented Oriental Melon for Fermentation Use (참외주 제조를 위한 이상발효 참외의 원료전처리 및 저장조건의 확립)

  • Kim, Tae-Young;Lee, Sang-Ho;Kim, Jin-Sook;Kim, Sang-Bum
    • Applied Biological Chemistry
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    • v.49 no.3
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    • pp.202-208
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    • 2006
  • Since abnormal fermentation and short storage duration of oriental melon are the main problems causing loss in commercial value, it is necessary to develop a food processing method using uncommercial melon. In this study, we suggested the effective pretreatment and storage conditions of melon as the material for alcoholic beverage production. Abnormally fermented melon had smaller carbohydrate and larger moisture content than normal one, indicating that carbohydrate in normal melon was probably converted to fermented products during fermentation. The sugar content of oriental melon was increased after fruiting and the highest value $(12.4^{\circ}Brix)$ was found at 5 weeks of storage. The maximum storage duration of normal and fermented oriental melons were 25 and 7 days at $4^{\circ}C$, and 8 and 4 days at room temperature, respectively. The oriental melon for fermentation-use could be conserved after slicing for 30 days at $4^{\circ}C$ with the addition of 1.5% citric acid and for one year at $-20^{\circ}C$ with the plastic film sealing, respectively.

Development of Biologically Active Compounds from Edible Plant Sources XVIII. Isolation of Derivatives of Ergosterol from the Fruit Body of Phellinus linteus (식용 식물자원으로부터 활성물질의 탐색-XVIII. 상황버섯 (Phellinus linteus) 자실체로부터 Ergosterol 유도체의 분리)

  • Lyu, Ha-Na;Yoo, Jong-Su;Song, Myoung-Chong;Lee, Dae-Young;Kim, Dong-Hyun;Rho, Young-Duk;Kim, In-Ho;Baek, Nam-In
    • Applied Biological Chemistry
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    • v.50 no.1
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    • pp.57-62
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    • 2007
  • The fruiting body of Phellinus linteus was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2$O. The repeated silica gel and ODS column chromatographies of the EtOAc fraction led to isolation of four sterols. From the result of spectral data including NMR, MS and IR, the chemical structures of the sterols were determined as ergosta-7,24(28)-dien-3${\beta}$-ol (episterol, 1), 5${\alpha}$,8${\alpha}$-epidioxyergosta-6,9(11),22-trien-3${\beta}$-ol (dehydrop-eroxyergosterol, 2), 5${\alpha}$,8${\alpha}$-epidioxyergosta-6,22-dien-3${\beta}$-ol (ergoterol peroxide, 3), and $3{\beta}$,$5{\alpha}$-dihydroxy-6${\beta}$-methoxyergosta-7,22-diene (6-O-methylcerevisterol, 4). The ergosterols have been first isolated from this mushroom in this study.

Ectomycorrhizal Mushroom Occurrence around the Fairy Ring of Tricholoma matsutake at a Pine-Mushroom Forest (송이 발생림의 송이 균환 주변에 출현하는 외생균근성 균류)

  • Park, Hyun;Ka, Kang-Hyeon;Ryoo, Cheon-In;Kim, Kyo-Soo;Kim, Hyun-Joong
    • The Korean Journal of Mycology
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    • v.26 no.3 s.86
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    • pp.306-313
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    • 1998
  • The occurrences of ectomycorrhizal fungi were investigated in a pine-mushroom forest at Hongcheon, Korea. The fairy rings of Tricholoma matsutake were figured by the sporocarp places of T. matsutake for three years $(1995{\sim}1997)$, and the occurrences of other ectomycorrhizal fungi were surveyed with x and y dimensions for two years $(1996{\sim}1997)$. The diameters of fairy rings of T. matsutake ranged from 2m to 10m, which indicated that the age of the fairy rings as $10{\sim}50$ years when we consider that the growth of the fairy ring used to show about 10 cm per year. Russula bella, R. sororia, R. delica and Cantharellus minor were the major species occurred on the site during the survey period, and each species occupied 16.0%, 12.8%, 12.4% and 7.0% of total mushroom occurrence, respectively. From the results, we could conclude that the surveyed stand was a productivity-declining forest from the view point of pine-mushroom production. In addition, Amanita pantherina, Suillus bovinus, Ramaria flaccida and Laccaria amethystea were considered to be the indicator species for declining of pine-mushroom productivity since some fruiting bodies of the species appeared around the fairy ring of T. matsutake.

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Breeding and cultural characteristics of a newly bred Lentinula edodes strain, 'Bambithyang' (표고 신품종 '밤빛향'의 육성 및 특성)

  • Park, Youngae;Jang, Yeongseon;Ryoo, Rhim;Ka, Kang-Hyeon
    • Journal of Mushroom
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    • v.18 no.2
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    • pp.145-150
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    • 2020
  • A new cultivar, 'Bambithyang', was bred from monokaryotic strains of Sanbaekhyang and SANJO 707ho using the mono-mono hybridization method. Its incubation period was determined to be 100 days and its optimum temperature for fruit body flushing, 11-20℃. The mushrooms had a convex cap (diameter, 69.6 mm; thickness, 15.2 mm) and stipes in their center. The color of their upper sides was chestnut brown; their gills were arranged in a ripple style and had medium density. Their scales were cream-colored and fully distributed; the stipes were cream-colored and cylindrical (thick upper part). They also had cream colored fluffs and sporadic fruiting bodies. Zone lines were present when they were cultivated with mother and father varieties. Their stipe lengths and caps were 9% shorter and 16.9% thicker, respectively, than those of Sanbaekhyang.

Comparison of Growth and Fruit Setting Characteristics for Selecting the Optimum Winter-Planted Paprika Cultivars (겨울정식 파프리카의 적정 품종 선정을 위한 품종간 생육 및 착과 특성 비교)

  • Jang, Dong-Cheol;Choi, Ki-Young;Heo, Jae-Yun;Kim, Il-Seop
    • Horticultural Science & Technology
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    • v.34 no.3
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    • pp.424-432
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    • 2016
  • This experiment was carried out to compare growth and fruit setting characteristics in ten winter-planted paprika (Capsicim annuum L.) cultivars. Five red line paprika cultivars ('Maduro', 'Maranello', 'Nagano', 'Sirocco' and 'Special') and five yellow line paprika cultivars ('Coletti', 'Sven', 'Thialf', 'Volante' and 'Zagato') were used for this study. The experiment was performed for 42 weeks, from winter of 2013 to autumn of 2014. Based on the growth stages of paprika, growth characteristics were investigated six times, and each investigation was categorized from group 1 through group 6. The relative internode ratio showed a normal range at the early growth stage, but tended to gradually decline as growth progressed. This trend was greater in red line paprika than in yellow line paprika. Among the cultivars used for this experiment, 'Special' and 'Zagato' showed growth inhibition, whereas 'Maranello' and 'Volante' kept a balanced growth during summer cultivation. The fruit set percentage in yellow paprika was 10.8% higher than in red paprika, while the number of branches in yellow paprika was 4.1% less than in red paprika. When measured after the full fruiting age, from June to July, the number of fruit set in group 4 was much lower in 'Sirocco' and 'Coletti' than in any of the other cultivars, indicating that they were more sensitive to the growth environment during the rainy season. These findings suggest that 'Maranello', 'Nagano', 'Sven', 'Thialf', and 'Volante' could be effectively used for summer cultivation in a high-plastic-film greenhouse, and 'Maranello', 'Special', 'Volante', and 'Zagato' could be good candidates for a low-plastic-film greenhouse.

Effects of Agaricus blazei Murill on Lipid Metabolism in Rats Fed High Fat Diet (신령버섯이 고지방 식이를 급여한 흰쥐의 지질대사에 미치는 영향)

  • 오세원;이충언;고진복
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.5
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    • pp.821-826
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    • 2004
  • The effects of the fruiting body of Agaricus blazei Murill on the weight gains, food intakes, food efficiency ratios, serum and hepatic lipids concentrations were investigated in male rats. Sprague-Dawley rats, 21 weeks old, were given four different types of diets for a succeeding period of 10 weeks: either a normal diet (5% corn oil), a high fat diet (high fat; 20% lard), a 3% or 5% Agaricus diet (high fat diet+3% or 5% Agaricus powder). The body weight gains and food efficiency ratios of the rats fed 5% Agaricus diet were significantly lower than those of the rats fed high fat diet. The hepatic and kidney weights of the rats fed Agaricus diets were similar to those of the rats fed high fat diet. The epididymal fat pad weights of the rats fed 3% or 5% Agaricus diets were significantly lower than those of the rats fed high fat diet. The concentrations of hepatic and serum triglyceride in the rats fed 5% Agaricus diet were significantly lower than those in the rats fed high fat diet. But the hepatic total cholesterol of rats fed the 3% or 5% Agaricus diets were similar to those of rats fed the high fat diet. The concentrations of serum total cholesterol, LDL-cholesterol and atherogenic index in rats fed the 3% or 5% Agaricus diets were significantly decreased compared with those of rats fed the high fat diet. The HDL-cholesterol/total- cholesterol ratios of the rat fed 3% or 5% Agaricus diet were significantly increased compared with those of rats fed the high fat diet. There were no differences in serum concentrations of HDL-cholesterol and phospholipid among the experimental groups. These results showed that the 5% Agaricus diet feeding decreased the total cholesterol, the triglyceride, the LDL-cholesterol and the atherogenic index, and increased the HDL-cholesterol/total-cholesterol ratio in serum of rats.

An Analysis on Site, Soil and Cultivation Characteristics of Korean Mountain Cultivated Ginseng (Panax ginseng) Field (산양삼 재배지의 입지, 토양 및 재배특성 분석)

  • Kwon, Su-Deok;Kang, Jeong-Hee;Yoon, Jun-Hyuck;Moon, Hyun-Shik
    • Journal of agriculture & life science
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    • v.45 no.6
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    • pp.81-88
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    • 2011
  • This study was carried out to provide basic information for standard cultivation guideline of Korean mountain cultivated ginseng (Panax ginseng). Environmental conditions, soil properties, growth process, cultivation methods and sowing timing were investigated in cultivations of Korean mountain cultivated ginseng in Korea (18 region in 9 province). Most of Korean mountain cultivated ginseng was cultivated to north, north-east, east or north-west direction in broad-leaved forest, IV age group, and 500~1,000 trees/ha. Elevation was below 500 m and slope was below $25^{\circ}$ in cultivation. There were significant differences in soil properties by region and cultivation based on organic matter (3.43~24.07%) and pH level (3.8~5.7). Sprouting, leaf unfolding, flowering, fruiting, red ripening, fruit drop and leaf drop of Korean mountain cultivated ginseng was investigated in cultivation. The results show that it took 98 days on average for the whole growth process. There were two methods, sowing and transplant for cultivation. Sowing methods were classified in sowing after opening-testa treatment and direct sowing. and it was 70% and 30%, respectively.

Analysis of General Components and Vitamin and Mineral Contents of the Mushroom Agrocybe chaxingu (차신고버섯(Agrocybe chaxingu)의 일반성분, 비타민 및 미네랄 함량분석)

  • Lee, Kwang-Jae;Yun, In-Jue;Kim, Hee-Yeon;Park, Yu-Hwa;Ham, Hun-Ju;Park, Young-Hak;Joo, Jin-Ho;Lim, Sang-Hyun;Kim, Kyung-Hee
    • Food Science and Preservation
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    • v.16 no.4
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    • pp.549-553
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    • 2009
  • The mushroom Agrocybe chaxingu was analyzed to evaluate the nutritional value of this potential food. The moisture content was 88.9% in the fruiting body of A. chaxingu, and the proportions of ash, crude fiber, carbohydrate, crude protein, and crude fat were 0.9%, 1.4%, 7.2%, 2.5% and 0.4% by weight, respectively. A. chaxingu contained 1.29 mg/100 g edible weight of vitamin $B_1$, and the contents of vitamin $B_2$, vitamin $B_3$, and vitamin C were 0.15 mg, 0.32 mg, and 18.4 mg per 100 g of wet mushroom. Potassium (3,318 mg/100 g, dry basis) was found at the highest concentration in A. chaxingu. In addition, the mushroom contained many other minerals (all figures are mg per 100 g of dried mushroom) such as phosphorus (909.7), magnesium (141.3), sodium (12.7), zinc (7.4), iron (6.5), copper (2.8), manganese (0.8), and nickel (0.1). The results indicate that A. chaxingu is a valuable nutrient source.

Cultivation of Paecilomyces tenuipes using Mini-kit, small culture container (소규모 재배상을 이용한 생동충하초 재배)

  • Nam, Sung-Hee;Lee, Kwang Gill;Yeo, Joo Hong;Lee, Heui Sam;Hwang, Jae Sam;Choi, Young-Cheol;Park, Kwan-Ho
    • Journal of Sericultural and Entomological Science
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    • v.50 no.2
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    • pp.116-121
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    • 2012
  • Cordyceps and its allies fungi has been described as a secret medicine that gives eternal youth and a long life. Some species of Cordyceps are sources of biochemicals, such ascordycepin, with interesting biological and pharmacological properties. Hence, it has been studied to uncover its pharmacological effect. We attempted to study the formation of fruiting bodies and to develop means of mass production Korean isolate of Paecilomyces tenuipes has been inoculated into silkworms, where it reproduced using culture container, mini-kit successfully. Culture container, mini-kit is composed of a cylinder-shaped body and lid. The container is made of translucent polyethylene terephthalate. The size of the container is $82{\times}75mm$, reduced by 10 times as compared with the conventional culture kit. The mini kit has many advantages - high culture amount, ability of maintaining optimal humidity, parasite-free cultivation and high-end appearance. With the kit, the optimal cultivation condition is under $22^{\circ}C$, culture period of 53 days. And synnemata of P. tenuipes could be kept fresh for 14 days at the temperature of under $10^{\circ}C$. Therefore, the Min-kit can be used in both ways as a culture container and a packing kit for end-user customers.