• The Korean Journal of Food And Nutrition
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• v.29 no.1
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• pp.33-36
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• 2016

Carbonic anhydrase is essential for the cellular transportation of hydrogen and bicarbonate ions and plays a key role in a wide variety of physiological processes. Rainbow trout, Oncorhynchus mykiss is an important freshwater fish in aquaculture industry and is known to be one of the most susceptible species to environmental contamination. In this study, carbonic anhydrase was detected in the kidney and intestine of rainbow trout. Carbonic anhydrase was isolated from cytosolic proteins and identified by using SDS-PAGE, isoelectric focusing, and immunohistochemical methods. A specific protein band with molecular weight of 30 kDa and pI of 7.0 was detected by Western blotting. The immunohistochemical results showed that carbonic anhydrase was located at various cells in the kidney and intestine of rainbow trout.

• Animal cells and systems
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• v.2 no.4
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• pp.521-527
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• 1998

The turnover of epidermal cells after seawater adaptation of the freshwater fishwas studied in the guppy (Poecilia reticulata) by means of Proliferating cell nucleus antigen (PCNA) immunocytochemistry and transmission electron microscopy. The number of PCNA-immunoreactive cells in the epidermis of the seawater-adapted guppies, which becomes thinner than that in the fresh-water, generally increases four times as much. Degeneration of filament-containing cells by necrosis or apoptosis occurs mainly in epidermal cells. Apoptotic filament-containing cells seem to be shed into the water in the environment instead of phagocytosis by adjacent macrophages. The apoptotic chloride cell has a highly condensed cytoplasm and the lumen of tubular system is distended. The apoptotic mucous cell, which has an electron-dense cytoplasm, is characterized by the presence of a large multivesicular body of different electron densities. Macrophages contain many electron-dense lysosomal bodies and large vesicles filled with cellular debris. It is concluded that mitosis and apoptosis of epidermal cells are greatly stimulated when fish are adapting to seawater. This result reflects an increase in epidermal cell turnover by change of environmental salinity.

• Journal of Korean Academy of Rural Health Nursing
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• v.2 no.1
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• pp.33-42
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• 2007

Purpose: This study was done to determine the extent of Clonorchis sinensis infections as well as other parasitic infections and to determine the growth rate. Method: Analyzed test results were from 1,050 bowel samples taken at 3 public health subcenters located in Muju, North Jeolla Province during February and March 2007. Result: The results are summarized as follows; The egg positive rate by stool examination was 4.3%. Ascaris lumbricoides, Enterobius vermicularis, Clonorchis sinensis, Trichuris trichiura, Metagonimus, Paragonimus were detected. No one was infected with multiple parasites. Infection rate for Clonorchis sinensis was 3.5%. Statistical correlation to Clonorchis sinensis infection rate was as follows; Gender (p=0.001), Age (p=0.005), Proximity to a river (p<0.000), Drinking (p<0.000), Smoking (p=0.009), Cooking of freshwater fish (p<0.000), Confidence in subjective health status (p=0.032), Family history of infection (p=0.005), No significant correlation was found between Clonorchis sinensis infection rate and duration of stay (p=0.809) in the agricultural communities or between Clonorchis sinensis infection and knowledge of Clonorchis sinensis (p=0.113). Conclusion: The results of this survey show that there is a need to develop a program to test the condition of Clonorchis Sinensis infection and its growth rate in residents of rural communities.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.4
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• pp.396-405
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• 2017

We observed the osteological development in larvae and juveniles of Korean spotted sleeper Odontobutis interrupta bred in the laboratory in April 2014. Immediately after hatching, the prelarvae, which were about 4.27 mm long, showed ossification of the premaxillary bones in the upper jaw and the dentary and articular bones in the lower jaw. At 7.11 mm, the larvae showed complete fusion of the post-cleithra and ossification of the scapulae with the appearance of one hole. At 8.65 mm, the larvae showed ossification of seven ribs from the third abdominal vertebra and an increase in the length of the neural spine and hemal spine. The number of caudal fin rays increased to 19. At 11.9 mm, the juveniles showed ossification of three procurrent rays on the side of the parhypural bone as well as ossification of two procurrent rays on the side of the epural bone, indicating the complete ossification of all spicules.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.16 no.6
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• pp.119-133
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• 2013

The purpose of this study is to develop riverbed techniques to activating ecological function of hyporheic zone. Hyporheic zone maybe simply defined as an active eco-tone between surface water and groundwater, which facilitates to exchange water, nutrients and aquatic habitat occur in response to variation in discharge and bed geomorphology. The aim of this study is to evaluate the effectiveness of an applied riverbed technique for two years since its installation in the hyporheic zone. The experimental riverbed technique has been implemented on Anyang stream penetrating Anyang city in Gyunggi province. The dimension of the installed structure is 5.0 m in width, 46 m in length. Bottom layer is filled with rip-rap covered with gabion. After the implementation of the technique, the study conducts follow-up monitoring in two years of between 2011 and 2012. The results of follow-up monitoring for two years are as follows:1) In Hydro geomorphic process, the riverbed technique maintains hydraulic stability despite of several flood events in 2011, 2012. 2) After transformation to form pool-and-riffle habitat, for aquatic community composed of freshwater fish, macro invertebrate, and attached algae, the species diversity and population gradually increased. 3) The riverbed technique achieved desired effect on enhancement of ecological function in hyporheic zone.

• Parasites, Hosts and Diseases
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• v.48 no.3
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• pp.259-261
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• 2010

Human Gnathostoma hispidum infection is extremely rare in the world literature and has never been reported in the Republic of Korea. A 74-year-old Korean man who returned from China complained of an erythematous papule on his back and admitted to our hospital. Surgical extraction of the lesion and histopathological examination revealed sections of a nematode larva in the deep dermis. The sectioned larva had 1 nucleus in each intestinal cell and was identified as G. hispidum. The patient recalled having eaten freshwater fish when he lived in China. We designated our patient as an imported G. hispidum case from China.

• Journal of The Korean Society of Clinical Toxicology
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• v.11 no.1
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• pp.46-48
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• 2013

Potamotrygon motoro, also known as the Marble motoro, is a potamodromous freshwater ray native to the basins of the Amazon River. Marble motoros were introduced to South Korea in the 2000s, and, because they are easy to raise, were sold as aquarium fish. The aim of this report is to illustrate a new case involving envenomation by a Marble motoro. A 35-year-old commercial aquarium assistant came to the hospital after being pricked by a Marble motoro. The clinical picture in this case showed acute local pain with minimal systemic manifestations. This patient recovered after receiving symptomatic treatment and wound care. This case of envenomation accentuates the potential for injury among people coming in contact with a venomous Marble motoro.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.40 no.4
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• pp.183-186
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• 2007

The effect of live food and commercial diet on sweetfish Plecoglossus altivelis culture was assessed by determining the daily growth rate and feed conversion ratio. After feeding for 8 weeks, the Moina macrocopa diet resulted in a higher growth rate of 5.101 compared to commercial (3.441) and Artemia (2.729) diets. In addition fish fed the M. macrocopa diet had the weight gain, averaging 41.29 g at the end of the experiment. The content of n-3 highly unsaturated fatty acids (HUFA) was highest in the commercial diet, while free fatty acids were not detected in the M. macrocopa diet. Triglycerides, the energy source in fishes, constituted 40.0% of the M. macrocopa diet, which was significantly higher than in the commercial and Artemia diets at 20.0 and 12.0%, respectively These results indicate that freshwater M. macrocopa can be used as feed for sweetfish larvae, even without docosahexaenoic acid (DHA) enrichment from Artemia.

• Research in Community and Public Health Nursing
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• v.23 no.4
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• pp.427-437
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• 2012

Purpose: In order to lower the infection rate and the reinfection rate of Clonorchiasis in high-risk areas, we performed and analyzed a case management on people with chlonorchisis. Methods: The data was collected from April 2010 to March 2011. A community health practitioner was selected as a case manager based on our training program. The intervention group had 58 participants with three months of case management and the control group had 144 participants handled with traditional methods. Results: the pre and post-test in the intervention group and the control group showed us improved knowledge of Clonorchiasis, attitudes and behavior toward eating freshwater fish. The results of post-test between the intervention group and the control group showed statistically significant differences in all categories, except a hand-washing category. However, one year after a case management, there was no significant difference in the failure rate of treatment. Conclusion: The case management was effective for people infected with Clornorchiasis near riverside areas; however, in order to lower the rates of reinfection and treatment failure, it is necessary to perform continuous monitoring and regular evaluations.

• Journal of the East Asian Society of Dietary Life
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• v.20 no.5
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• pp.785-793
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• 2010

This study aimed to identify particular foods that would be most appropriate as representatives foods of Daejeon, as well as the key factors affecting the branding of image of those foods. To achieve these aims we carried out a survey amongst 1,600 citizens and visitors. The Daejeon representative foods were determined to be Gujeuk Dotorimuk (Gu-jeuk acorn jelly), Hanbat Seolleongtang, Dolsotbap (stone pot mixed rice), Sutgol naengmyeon (Sutgol Cold Noodles), Daecheongho Minmulmaeuntang (Daecheong lake freshwater spicy fish soup) and Samgyetang (casserole of spatchcock with ginseng). These six dishes were designated as representative foods in 2001. Findings from the survey indicated the lowest level of brand! dish awareness was 11.6% generally and the top two preferences for the representative foods were ranked as, Samgyetang (24.8%) and Dolsotbap (23.6%). According to an awareness survey age differences played a significant role. Adults displayed a much higher awareness than younger generations. The survey also found that Daejeon city was strongly associated with images of science and technology, particularly areas such as Expo and the Daedeok Valley. This study's results suggests that developing a menu and marketing strategy based upon associated regional images may increase awareness for local foods among young consumers.