Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
권호 표지

Induced Epidermal Cell Turnover in the Seawater-Adopted Guppy, Poecilia reticulata

  • Wha Sun Kang (Department of Biology, College of Medicine, The Catholic University) ;
  • Young Wha Moon (Department of Biology, College of Medicine, The Catholic University) ;
  • Ji Won Han (Department of Biology, College of Medicine, The Catholic University) ;
  • No Gi Park (Department of Biology, College of Medicine, The Catholic University) ;
  • Han Hwa Kim (Department of Biology, College of Medicine, The Catholic University)
  • Published : 1998.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

The turnover of epidermal cells after seawater adaptation of the freshwater fishwas studied in the guppy (Poecilia reticulata) by means of Proliferating cell nucleus antigen (PCNA) immunocytochemistry and transmission electron microscopy. The number of PCNA-immunoreactive cells in the epidermis of the seawater-adapted guppies, which becomes thinner than that in the fresh-water, generally increases four times as much. Degeneration of filament-containing cells by necrosis or apoptosis occurs mainly in epidermal cells. Apoptotic filament-containing cells seem to be shed into the water in the environment instead of phagocytosis by adjacent macrophages. The apoptotic chloride cell has a highly condensed cytoplasm and the lumen of tubular system is distended. The apoptotic mucous cell, which has an electron-dense cytoplasm, is characterized by the presence of a large multivesicular body of different electron densities. Macrophages contain many electron-dense lysosomal bodies and large vesicles filled with cellular debris. It is concluded that mitosis and apoptosis of epidermal cells are greatly stimulated when fish are adapting to seawater. This result reflects an increase in epidermal cell turnover by change of environmental salinity.

Keywords

References

  1. Eur J Histochem v.38 PCNA/cyclin expression and BrdU uptake define proliferating myosatellite cells during hyperplastic muscle growth of fish (Cyprinus carpio L.) Alfei L;Onali A;Spano L;Colombari PT;Altavista PL;de Vita R
  2. J Exp Zool v.241 Gill morphology and sodium influx in the rainbow trout (Salmo gairdnerr) acclimated to artificial freshwater environments Avella M;Masoni A;Bornancin M;Mayer-Gostan N
  3. J Fish Biol v.34 Histomorphological changes in some organs of the brown bullhead, Ictalurus nebulosus Lesueur, following short- and long-term exposure to copper Benedetti I;Albano AG;Mola L
  4. Nature v.326 Cyclin/PCNA is the auxiliary protein of DNA polymerase-delta Bravo R;Frank R;Blundell PA;McDonald BH
  5. Virchows Arch v.50 Quantitative histological and histochemical studies on the occurrence and stages of controlled cell death (apoptosis) during regression of rat liver hyperplasia Bursch W;Taper HS;Lauer B;Schulte-Hermann R
  6. Biol Cell v.56 Cell renewal and differentiation in the gill epithelium of fresh- or salt-water-adapted euryhaline fish as revealed by $[{^3H}]$-thymidine autoradiography Chretien M;Pisam M
  7. Vet Pathol v.21 Acute pathological effects of inorganic mercury and copper in gills of rainbow trout Daoust PY;Wobeser G;Newstead JD
  8. J Histotechnol v.14 Detection and evaluation of proliferating cell nuclear antigen (PCNA) in rat tissue by an improved immunohistochemical procedure Foley JE;Dietrich DR;Swenberg JA;Maronpot RR
  9. Science v.215 The chloride cell: definitive identification as the salt-secretory cell in teleosts Foskett JK;Scheffey C
  10. J Fish Biol v.33 Cellular responses in the skin of carp maintained in organically fertilized water Iger Y;Abraham M;Dotan A;Fattal B;Rahamim E
  11. Cell Tissue Res v.275 Cellular responses of the skin carp (Cyprinus carpio) exposed to acidified water Iger Y;Wendelaar Bonga SE
  12. Korean J Electron Micros v.26 Apoptosis in chloride cells of killifish (Orizias latipes) gills adapted to the seawater Kang WS;Moon YW;Han JW;Kim HH
  13. New Medical J v.31 Epidermal fine structure of the killifish, Aplochelius latipes Kim HH;Chi YD;Moon YW;Kang WS
  14. Korean J Zool v.36 Ultrastructural changes of chloride cells of the guppy (Poecilia reticulata) gill according to the environmental salinity Kim HH;Chi YD;Moon YW;Kang WS
  15. Exp Cell Res v.166 Expression of proliferating cell nuclear antigen (PCNA/cyclin) during the cell cycle Kurki P;Vanderlaan M;Dolbeare F;Gray J;Tan EM
  16. Cell Tissue Res v.240 The role of environmental sodium chloride relative to calcium in gill morphology of fresh-water salmonid fish Laurent P;Hobe H;Dunel-Erb S
  17. Cell Tissue Res v.236 Morphological and morphometricall changes in chloride cells of the gills of Pimephelas promelas after chronic exposure to acid water Leino RL;McCormick JH
  18. Can J Zool v.58 Cell renewal in the gills of the fish Barbus conchonius Mackinnon M;Enesco HE
  19. Nature v.309 Identity of the proliferating cell nuclear antigen and cyclin Mathews MB;Bernstein RM;Franza BR;Jr.;Garreles JI
  20. Can J Zool v.61 The effects of H upon the gills of freshwater fish McDonald DG
  21. J Immunol v.121 Autoantibody to a nuclear antigen in proliferating cells Miyachi K;Fritzler MJ;Tan EM
  22. Korean J Zool v.38 Ultrastructural and histochemical changes of mucous cells in the gill epithelium of the seawater-adapted guppy (Poecilia reticulatus) Moon YW
  23. Korean J Electron Micros v.25 Ultrastructural changes of the epidermis of guppy (Plecilia reticulatus) scale adapted to the seawater Moon YW
  24. Neurosci Res v.18 Fibroblast growth factor induces proliferating cell nuclear antigen-immunoreactive cells in goldfish retina Negishi K;Shinagawa S
  25. Cell Mol Neurobiol v.11 Induction of proliferating cell nuclear antigen (PCNA)-immunoreactive cells in goldfish retina following intravitreal injection with 6-hydroxydopamine Negishi K;Stell WK;Teranishi T;Karkhanis A;Owusu-Yaw V;Takasaki Y
  26. Biotech Histochem v.69 Detection of proliferating cell nuclear antigen in tissues of three small fish species Ortego LS;Hawkins WE;Walker WW;Krol RM;Benson WH
  27. Nature v.234 Fluid friction of fish slimes Rosen MW;Cornford NE
  28. Cell Tissue Res v.229 Ultrastructural changes in hatching-gland cells of pike embryos (Exos Iucius L.) and evidence for their degeneration by apoptosis Schoots AFM;Evertse PACM;Denuce JM
  29. Gen Comp Endocrinol v.38 Morphometrical studies of the ultrastructure of the epidermis of the guppy, Poecilia reticulata Peters, following adaptation to seawater and treatment with prolatin Schwerdtfeger WK
  30. J Fish Biol v.21 Changes in the mucous cells of gills, buccal cavity and epidermis of the nine-spined stickle-back, Pungitius pungitius L., introduced by transferring the fish to seawater Solanki TG;Benjamin M
  31. Cell Tissue Res v.259 The ultrastructure of chloride cells in the gills of the teleost Oreochromis mossambicus during exposure to acidified water Wendelaar Bonga SE;Flik G;Balm PHM;van de Meij JCA
  32. Cell Tissue Res v.221 Effects of external osmolarity, calcium and prolactin on growth and differentiation of the epidermal cells of the cichlid teleost Sarotherodon mossambicus Wendelaar Bonga SE;Meis S
  33. Vertebrate Endocrinology: Fundamentals and Biomedical Implications, Vol.1 Stannius corpuscles Wendelaar Bonga SE;Pang PKT;Pang PKT(ed);Schreibman MP(ed)
  34. Cell Tissue Res v.255 Degeneration and death, by apoptosis and necrosis, of the pavement and chloride cells in the gills of the teleost Oreochromis mossambicus Wendelaar Bonga SE;van der Meij CJM
  35. Toxicol Pathol v.20 Histopathological effects in Poecilia reticulata (guppy) exposed to methyl mercury chloride Wester PW;Canton HH
  36. J Zool Lond v.160 The skin surface of bony fishes Whitear M
  37. Biology of the Integument, Vol.2. Verebrates v.2 The skin fishes including cyclostomes epidermis Whitear M;Bereiter HJ(ed);Matoltsy AG(ed);Richards KS(ed)
  38. Cell Death in Biology and Pathology Cell death: a new classification separating apoptosis from necrosis Wyllie AH;Bowen ID(ed);Lockshin RA(ed)
  39. Inst Rev Cytol v.68 Cell death: the significance of apoptosis Wyllie AH;Kerr JFR;Currie AR
  40. Comp Biochem Physiol v.86A Epithelial and pillar cell replacement in gills of juvenile trout, Salmo gairdneri Richardson Zenker WGE;Ferguson HW;Barker IK;Woodward B