• 약학회지
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• 제58권4호
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• pp.245-249
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• 2014

Murrayafoline-A (1-methoxy-3-methylcarbazole) is a monomeric carbazole alkaloid found in Murraya euchrestifolia HAYATA and Glycosmis stenocarpa. We have recently shown that murrayafoline-A has positive inotropic effect in isolated rat ventricular myocytes. To know possible mechanisms for the positive inotropic effect of murrayafoline-A we examined the effects of murrayafoline-A on in situ behavior of cardiac $Ca^{2+}$ release units ('$Ca^{2+}$ sparks') and sarcoplasmic reticulum (SR) $Ca^{2+}$ loading using confocal $Ca^{2+}$ imaging method in single rat ventricular myocytes. Murrayafoline-A significantly increased the frequency (events/($10^3{\mu}m^2{\cdot}s$)) of $Ca^{2+}$ sparks in a concentration-dependent manner, with an $EC_{50}$ of $28{\pm}6.4{\mu}M$ and a maximal ~twofold change. The $Ca^{2+}$ content in the SR, measured as caffeine (10 mM)-induced $Ca^{2+}$ transient, was significantly increased by murrayafoline-A (${\approx}$116% and ${\approx}$123% of control at 25 and 100 ${\mu}M$, respectively). In addition, murrayafoline-A significantly increased the fractional $Ca^{2+}$ release, suggesting increase in the efficacy of $Ca^{2+}$ release at given SR $Ca^{2+}$ loading. These results suggest that murrayafoline-A may enhance contractility via increase in $Ca^{2+}$ release from the SR through the ryanodine receptors in ventricular myocytes.

• Journal of Acupuncture Research
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• 제19권4호
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• pp.167-182
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• 2002

This experiment was designed to investigate the effects of electroacupuncture (EA) on chronic pains and factors that affected EA effects. The responses of wide dynamic range (WDR) cells to electrical stimulation of $A{\delta}$ & C afferent fibers were used as an index of pain in rats with chronic pains induced by intraplantar injection of complete Freund's adjuvant or peripheral nerve injury. In rats with chronic pains, low (2Hz) and high (100Hz) frequency EA stimulation applied to zusanli caused the inhibition of WDR cell responses in about 60% of rats and the inhibitory actions were dependent on the stimulus strength. EA stimulation also induced an excitation of WDR cell responses in 23.9% of rats and no effect in 15.8% of rats. However, it seemed that in normal rats compared to the rat with chronic pains, the incidence of which EA stimulation caused the excitation or no effect was high. Reversible spinalization almost completely blocked EA-induced inhibitory or excitatory effects. EA stimulation more frequently induced the excitation of WDR cell responses in lightly anesthetized (0.6%) rats and the enhanced responses of WDR cells were inhibited by EA stimulation in the rat anesthetized with 1.5% enflurane. These experimental findings suggest that in rats with chronic pain, EA stimulation inhibited WDR cell responses to slow $A{\delta}$ and C fiber stimulation and EA-induced inhibitory action was under the control of descending inhibitory system and degree of anesthesia.

• 한국자동차공학회논문집
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• 제3권1호
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• pp.1-12
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• 1995

Isothermal and thermal-mechanical fatigue characteristics of 12Cr heat resisting steel used for high temperature applications were investigated including hold time effects. Isothermal low cycle fatigue test at $600^{\circ}C$ and in-phase, out-of-phase thermal-mechanical fatigue test at 350 to $600^{\circ}C$ were conducted using smooth cylindrical hollow specimen under strain-control with total strain ranges from 0.006 to 0.015. Regardless of thermal-mechanical and isothermal fatigue tests, cyclic softening behavior was observed and much more pronounced in the thermal-mechanical fatigue tests with hold times due to the stress relaxation during the hold time. The phase difference between temperature and strain in thermal-mechanical fatigue tests resulted in significantly shorter fatigue life for out-of-phase compared to in-phase. The differences in fatigue lives were dependent upon the magnitudes of plastic strain ranges and mean stresses. During the hold time in the strain-controlled fatigue tests, the increase in the plastic strain range and the stress relaxation were observed. It appeared that the increase in plastic strain range per cycle and the introduction of creep damage made important contributions to the reduction of thermal-mechanical fatigue life with hold time, and the life reduction tendency was more remarkable in the in-phase than in the out-of-phase thermal-mechanical fatigue. Isothermal fatigue tests performed under the combination of fast and slow strain rates at $600^{\circ}C$ showed that the fatigue life decreased as the strain rate and frequency decreased,especially for the low strain ranges.

• 생물정신의학
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• 제15권3호
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• pp.186-193
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• 2008

Objectives : The authors tried to investigate the characteristics of Heart Rate Variability(HRV) among the subjects with metabolic syndrome for testing the possibility of clinical application of HRV in evaluating metabolic syndrome. Methods : We examined the difference of the means of HRV between 122 subjects of metabolic syndrome and 1057 healthy controls using T-test, and the change of means in HRV according to increasing risk factors of metabolic syndrome. Using multiple regression analysis, we examined the association of HRV with the risk factors of metabolic syndrome. Results : The HRV indices including SDNN(Standard Deviation of all normal NN intervals), RMSSD(the square Root of the Mean Squared Differences of successive all normal NN interval), TP(Total Power), LF(Low Freuency), and HF(High Frequency) were significantly lower in the metabolic syndrome group than in the normal control group, and the means of all HRV indices except LF/HF were decreased consistently according to increasing risk factors of metabolic syndrome. The fasting glucose, triglyceride, and waist circumference were dependent factors that contributed significantly to the change of HRV. Conclusion : These findings suggest metabolic syndrome adversely affects cardiac autonomic system and HRV could be a useful method for evaluating metabolic syndrome.

• 한국가시화정보학회지
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• 제14권1호
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• pp.57-61
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• 2016

We report a finding of fast(exceeding 2,000 K/s) heating of polydimethylsiloxane(PDMS), one of the most commonly-used microchannel materials, under cyclic loadings at high(~MHz) frequencies. A microheater was created based on the finding. The heating mechanism utilized vibration damping of sound waves, which were generated and precisely manipulated using a conventional surface acoustic wave(SAW) microfluidic system, in PDMS. The penetration depths were measured to range from $210{\mu}m$ to $1290{\mu}m$, enough to cover most microchannel heights in microfluidic systems. The energy conversion efficiency was SAW frequency-dependent and measured to be the highest at around 30 MHz. Independent actuation of each interdigital transducer(IDT) enabled independent manipulation of SAWs, permitting spatiotemporal control of temperature on the microchip. All the advantages of this microheater facilitated a two-step continuous flow polymerase chain reaction(CFPCR) to achieve the billion-fold amplification of a 134 bp DNA amplicon in less than 3 min. In addition, a technique was developed for establishing dynamic free-form temperature gradients(TGs) in PDMS as well as in gases in contact with the PDMS.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2000년도 Proceedings of 2000 KSAM International Symposium and Spring Meeting
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• pp.178-181
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• 2000

In order to develop the novel gene expression system, we introduced new control elements which could influence the foreign gene expression in animal cells. When the foreign genes are introduced into the genome of higher eukaryotic cells, the expressions from these integrated genes are often low and can vary greatly depending on the positions of the integration sites due to the complex nature of the chromatin structures (1). First we screened the various DNA sequence elements which can function as an insulator of gene expression from these position effects and can cooperate with the SV40 enhancer/promoter. Among the several DNA elements from the various sources, we identified the particular DNA element which confers the increased frequency of the positive colonies, assayed by the reporter gene from stable selections indicating significantly reduced position effects. This element also showed the several fold-increased expression level as well as the copy-number dependent expression with host cell specificity. Second we modified the transcription termination element where we introduced the specific terminator in combination with SV40 polyA signal. This modified terminator showed the increased efficiency and the level of the gene expression. By combining these two elements, we made the animal cell expression system and tested successfully for the recombinant protein productions of TGF ${\beta}$-soluble receptor, Antithrombin III, and single chain Pro-Urokinase. [Supported by grants from MOCIE]

• 대한조선학회논문집
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• 제36권1호
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• pp.90-98
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• 1999

감쇠가 적은 금속 구조물의 공진에 의한 진동과 소음을 제어하기 위해 고무, 아스팔트 계열 등의 점탄성 물질이 다양하게 사용되고 있다. 일반적으로 점탄성재료를 이용한 감쇠처리는 점탄성재료의 하부를 모재인 금속층에 취부하고 점탄성재료의 상부를 구속층으로 적층하는 방법을 적용한다. 구속감쇠층이 취부된 구조물의 감쇠 및 강성 특성은 온도와 주파수뿐만 아니라 각 층의 두께에 따라 크게 달라진다. 따라서 구조물을 경량화하고 감쇠처리 효과를 극대화하기 위해서는 점탄성층과 구속층 두께의 최적설계가 매우 중요하다. 본 연구에서는 구속감쇠층을 가진 보의 점탄성층과 구속층의 두께 변화에 따른 손실계수의 변화를 면밀히 검토하고, 이를 토대로 지정된 손실계수를 가지되 감쇠층과 구속층의 최소두께를 결정하는 방법을 제시하였다. 제시된 방법의 타당성은 수치실험적 방법으로 검증하였다.

• 한국치위생학회지
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• 제7권1호
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• pp.61-72
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• 2007

The purposes of this study were to investigate the oral health status of the disabled children attending special schools in Seoul and Gyeonggi area, which were to collect baseline data for set up a oral health center in special schools. The study group comprised 915 disabled children aged 12~15 year. They were examined clinically and surveyed on 4 oral health related characteristics according to the type and level of disabilities The results may be summarized as follows: 1. DMFT index was the highest in children with crippled disorder. The plaque control was more required to the children with mental retardation or developmental disorder other than another types of disabilities. 2. About one third of the children with mental retardation or developmental disorder had their teeth brushed at least 3 times daily without any help. Over the half of the children with crippled disorder had their teeth brushed 2 times daily, 33.3% were independent and 38.9% totally dependent on help from others. 3. The more level of disabilities was higher, the more frequency of tooth brushing was lower and degree of dependance of the tooth brushing was higher. 4. There was tendency to visit to dental office more frequently for relief of dental symptoms in children with crippled disorder 5. The inability to call for help from others was the major barrier to dental care for the disabled.

• 한국통신학회논문지
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• 제29권10B호
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• pp.866-879
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• 2004

본 연구논문에서는 소비자의 서비스 품질에 따른 지불의사를 고려하여 음성/데이터 통합 이동통신서비스 제공업자의 수익을 극대화시키는 최적 가격을 결정하는 모델을 제시한다. 특히 고려되는 음성서비스 및 데이터서비스의 가격방식은 현재 우리나라 이동통신서비스의 회선요금제와 패킷요금제를 각각 적용한다. 이 모델은 소비자들이 핸드오프도중 발생하는 통화단절에 매우 민감하게 반응한다는 가정하에, 기지국이 무선채널을 할당함에 있어서 핸드오프 트래픽만이 독점사용할 수 있는 가드채널을 할당하는 방안을 고려하며, 더불어 서비스제공업자가 통화단절율의 서비스 품질을 소비자군에게 보장하는 경우를 고려한다. 궁극적으로 이 모델은 단기적으로 가격전략 및 무선채널 할당정책에 의존하여 서비스 품질을 개선$.$보장할 수 있는 방안을 제시하며, 그 결과 시스템자원의 확충없이는 수익과 서비스 품질의 개선$.$보장간에 상충관계가 존재함을 보여준다.

• 한국항공우주학회지
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• 제33권2호
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• pp.54-59
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• 2005

구동기의 백래쉬와 동강성을 고려한 미사일 조종날개의 비선형 공탄성 해석이 수행되었다. 아음속 비정상 공기력 계산을 위해 DHM을 사용하였고 최소상태접근법을 사용하여 근사하였다. 비선형 플러터 해석을 위해 백래쉬는 유격으로 모델하고 기술 함수법을 사용하여 선형화하였다. 또한, 동강성은 주파수의 함수로 모터의 운동방정식으로부터 계산하였다. 선형 및 비선형 플러터 해석 결과들은 공력탄성학적 특성들이 백래쉬와 동강성에 중요한 영향을 받는다는 것을 보여준다. 비선형 플러터 해석에서 다양한 제한 주기 운동이 선형플러터 속도 이하에서 관측되었다. 또한 플러터 특성과 응답을 시간영역에서도 조사하였다.