• Advances in Computational Design
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• v.2 no.2
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• pp.147-168
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• 2017

Over the past couple decades, externally bonded fiber reinforced polymer (FRP) composites have emerged as a repair and strengthening material for many concrete infrastructure applications. This paper presents an analytical investigation of the use of carbon FRP (CFRP) for a specific problem that occurs in concrete bridge girders wherein the girder ends are damaged by excessive exposure to deicing salts and numerous freezing/thawing cycles. A 3D finite element (FE) model of a full scale prestressed concrete (PC) I-girder is used to investigate the effect of damage to the cover concrete and stirrups in the end region of the girder. Parametric studies are performed using externally bonded CFRP shear laminates to determine the most effective repair schemes for the damaged end region under a short shear span-to-depth ratio. Experimental results on shear pull off tests of CFRP laminates that have undergone accelerated aging are used to calibrate a bond stress-slip model for the interface between the FRP and concrete substrate and approximate the reduced bond stress-slip properties associated with exposure to the environment that causes this type of end region damage. The results of these analyses indicate that this particular application of this material can be effective in recovering the original strength of PC bridge girders with damaged end regions, even after environmental aging.

• Computers and Concrete
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• v.21 no.2
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• pp.209-217
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• 2018

The dynamic compressive behavior of concrete after freezing and thawing tests are investigated by using the split Hopkinson pressure bar (SHPB) technique. The stress-strain curves of concrete under dynamic loading are measured and analyzed. The setting numbers of freeze-thaw cycles are 0, 25, 50, and 75 cycles. Test results show that the dynamic strength decreases and peak strain increases with the increasing of freeze-thaw cycles. Based on the Weibull distribution model, statistical damage constitutive model for dynamic stress-strain response of concrete after freeze-thaw cycles was proposed. At last, the fragmentation test of concrete subjected to dynamic loading and freeze-thaw cycles is carried out using sieving statistics. The distributions of the fragment sizes are analyzed based on fractal theory. The fractal dimensions of concrete increase with the increasing of both freeze-thaw cycle and strain rate. The relations among the fractal dimension, strain rates and freeze-thawing cycles are developed.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.77.2-78
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• 2003

An EREBP/AP2-type transcription factor (CaPFl) was isolated by DDRT-PCR following inoculation of soybean pustule pathogen Xanthomonas axonopodis pv. glycines Bra which induces HR on pepper leaves. Genomic Southern blot analysis revealed that the CaPFl gene is present as a single copy within the hot pepper genome. The deduced amino acid sequence of CaPFl has two potential nuclear localization signals, a possible acidic activation domain, and an EREBP/AP2 motif that could bind to a conserved cis- element present in promoter region of many stress-induced genes. The mRNA level of CaPFl was induced by both biotic and abiotic stresses. We observed higher-level transcripts in resistance-induced pepper tissues than diseased tissues. Expression of CaPFl is also induced upon various abiotic stresses including ethephon, MeJA, cold stress, drought stress and salt stress treatments. To study the role of CPFI in plant, transgenic Arabidopsis and tobacco plants which express higher level of pepper CaPFl were generated. Global gene expression analysis of transgenic Arabidopsis by cDNA microarray indicated that expression of CaPFl in transgenic plants affect the expression of quite a few GCC box and DRE/CRT box-containing genes. Furthermore, the transgenic Arabidopsis and tobacco plant, expressing CaPFl showed tolerance against freezing temperature and enhanced resistance to Pseudomonas syrnigae pv. tabaci. Taken together, these results indicated that CaPFl is a novel EREBP/AP2 transcription factor in hot pepper plant and it may has a significant role(s) in regulation of biotic and abiotic stresses in plant.

• The Journal of Korean Academy of Prosthodontics
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• v.26 no.1
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• pp.153-169
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• 1988

There is a little scientific documentation reporting the stress, distribution to the edentulous mandible by different concepts of occlusal scheme. So, this study was to investigate the hypothesis that the magnitude and distribution of the occlusal stresses, transmitted through a mandibular complete denture base to the edentulous mandible, would be influenced by the lingualized occlusion. This investigation was performed to analyze the stresses induced in a three-dimensional photoelastic edentulous mandible, when a load is applied to the denture arranged into lingualized occlusion in centric relation, lateral and protrusive functional position. The mounted denture on a Dentatus Type ARO articulator was loaded in a pure vertical direction with 15kgs on the center of articulator in each case and the stresses were frozen into epoxy edentulous model at $127^{\circ}C$ in the stress freezing furnace. The stress-frozen epoxy models were sliced with diamond disc saw into 4mm thick. The slices were examined with a circular polariscope. The results were as follows: 1. In centric relation, the stresses were low at anteriors, and gradually increase to the premolar, molar area and highest at the first molar and gradually decrease from the second molar and lowest at the retromolar pad region. The lingual side showed higher stresses than labiobuccal side. 2. In lateral functional position, the working side showed higher stresses than the balancing side. In working side, the lingual side showed higher stresses than the buccal side and in balancing side, the buccal side showed higher stresses than the lingual side. 3. In protrusive position, stress distribution was symmetrical on the posteriors and the stresses were concentrated at the labial side of the anteriors.

• Journal of Plant Biotechnology
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• v.44 no.3
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• pp.287-295
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• 2017

Water temperature is one of the major factors that impacts the growth and life cycle of Pyropia tenera, one of the most valuable and cultivated marine red algae belonging to Bangiales (Rhodophytes). We analyzed transcriptome from gametophyte of P. tenera under normal and high temperature conditions, and identified four small heat shock proteins (sHSPs). They have no significant amino acid sequence homology with known proteins in public databases except PhsHSP22 from Pyropia haitanensis. PtsHSP19.3 gene responded to high temperature but slightly or not to desiccation, freezing or high salt condition. When the PtsHSP19.3 gene was overexpressed in Chlamydomonas reinhardtii, transformed Chlamydomonas lines revealed much higher growth rate than that of control cells under heat stress condition. Transformed cells also grew well in those of the control cell onto the medium containing high salt or $H_2O_2$. When the PtsHSP19.3 was fused to GFP and introduced into tobacco protoplast, fluorescence was detected at several spots. Results indicate that PtsHSP19.3 may form super-molecular assembles and be involved in tolerance to heat stress.

• Proceedings of the Korea Concrete Institute Conference
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• 1995.04a
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• pp.148-153
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• 1995

In this report, the physical and chemical properties of Che-ju aggregates were stedued, and the properties of concrete using 5 types combinations of Che-ju aggreagtes were compared with those of concrete using Dag-jeon area aggreagtes. As a result of the properties of Che-ju aggregates are very different with Dae-jeon area aggregates in many aspects. Especially, entrained air content of aggregate is over 1.5% so that the freezing & thawing resistance of concrete was caused in decrease. And the texture properties of Che-ju aggregates and a little content of the entrained air in mortar increase bonding stress between mortar and aggregate, as a result in improving the compressive strength of concrete. Meanwhile, the relationship between cement water ratio(C/W) and 28days compressive strength of concrete(F28) is derived from the stastical regression using experimental data as $F_{28} = -99 + 276 *($(C/W), so this eqation is useful for mix-design of concrete in Che-ju area.

• International Journal of Industrial Entomology and Biomaterials
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• v.27 no.1
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• pp.180-184
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• 2013

Recently, silk sericin has attracted the attention of researchers owing to its useful properties as a biomaterial including 1) good wound healing and cell activities, 2) fast gelation character, and 3) high water retention property. In the present study, silk sericin was prepared using different extraction times in hot water and the effect of extraction time on the rheological properties of sericin solutions and gels was examined. It was found that the production yield of sericin increased with extraction time. The shear viscosity of sericin solutions and gels decreased with increasing extraction time due to a decrease in sericin molecular weight. When the sericin solution transformed to a gel, the viscosity increased and the shear thinning behavior was more evident. In addition, the shear stress measurements indicated that the slip between the sericin samples and the measuring plate of the rheometer was increased by the gelation of sericin. The compression strength of sericin gel could be increased remarkably (by more than 100 fold) by preparation using the freezing and thawing method.

• Proceedings of the KSME Conference
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• 2003.04a
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• pp.1663-1666
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• 2003

TMA clathrate that is used by PCM of ice storage system in this research creates hydrate crystallization at higher temperature than pure water, and application is expected as PCM because having comparative big dormant temperature without phase separation phenomenon. In case this research uses TMA clathrate by PCM, choose admixture by purpose to control or remove subcooling of TMA clathrate and evaluated experimentally. Subcooling is improved and can expect contraction of freezing machine running time and increase of coefficient of performance as that add admixture to TMA clathrate conclusively. Also, may supply thermal storage system that apply low temperature potential heat thermal storage material that subcooling is improved more extensively laying stress on medium size building and small size building, can expect allowance through localization of ice storage system.

• Journal of Embryo Transfer
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• v.30 no.1
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• pp.1-6
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• 2015

The cold shock of spermatozoa is associated with oxidative stress induced by reactive oxygen species. This study was conducted to evaluate the toxicity of natural antioxidant green tea extract (GTE) in lactose-egg yolk (LEY) extender during boar sperm cooling prior to freezing. Spermatozoa were cooled to $5^{\circ}C$ for 3 h in LEY extender containing 0 (control), 1, 10, 100 or 1,000 mg/l of GTE, re-suspended with LEY-glycerol-Equex extender and cooled at $5^{\circ}C$ for 30 min. Sperm progressive motility, viability and phosphatidylserine (PS) translocation were evaluated. PS translocation was assayed by flow cytometry using Annexin V-FITC apoptosis detection kit. The sperm function including progressive motility, viability and PS translocation was not significantly different regardless of GTE concentrations (P>0.05). In conclusion, this study demonstrated non-toxicity of GTE supplement in LEY extender during sperm cooling.

• Geomechanics and Engineering
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• v.36 no.4
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• pp.391-406
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• 2024

In order to study the soil seasonal dynamic characteristics in the regions with four distinct seasons, the soil dynamic triaxial experiments were conducted by considering the environmental temperature range from -30℃ to 30℃. The results demonstrate that the dynamic soil properties in four seasons can change greatly. Firstly, the dynamic triaxial experiments were performed to obtain the dynamic stress-strain curve, elastic modulus, and damping ratio of soil, under different confining pressures and temperatures. Then, the experiments also obtain the dynamic cohesion and internal friction angle of the clay under the initial strain, and the changing rule was summarized. Finally, the results show that the dynamic elastic modulus and dynamic cohesion will increase significantly when the clay is frozen; as the temperature continues to decrease, this increasing trend will gradually slow down, and the dynamic damping ratio will go down when the freezing temperature decreases. In this paper, the change mechanism is objectively analyzed, which verifies the reliability of the conclusions obtained from the experiment.