• Clinical and Experimental Reproductive Medicine
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• v.27 no.2
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• pp.191-200
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• 2000

Objective: This study was carried out to compare the effects of the stepwise exposure treatments on the morphological normality, fertilization and blastocyst formation rate of the frozen-thawed mouse mature oocytes by vitrification or ultra-rapid freezing and to use as a fundamental data for the cryopreservation of human oocytes. Materials and Methods: The morphological normality and fertilization rates of the vitrified and ultra-rapid frozen mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were observed. After choosing the 3step exposure treatment groups, we observed the morphological normality and fertilization, blastocyst formation rate of the vitrified and ultra-rapid frozen mouse mature oocytes. Results: The morphological normality and fertilization rates of the vitrified mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were 75%, 85%, 88% and 58%, 61 %, 54% respectively. There were no significant differences among treatments(p>0.05). The morphological normality and fertilization rate of the control was 92% and 65%. There were no significant differences in fertilization rate among control and treatments (p>0.05). The morphological normality and fertilization rates of the ultra-rapid frozen mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were 83%, 83%, 84% and 75%, 63%, 56% respectively. There were no significant differences among treatments (p>0.05). The morphological normality and fertilization rate of the control was 95% and 67%. There were no significant differences among control and treatments (p>0.05). The morphological normality and fertilization rate of the vitrified or ultra-rapid frozen mouse mature oocytes after 3step exposure treatment were 69% and 75%, respectively. The blastocyst formation rate was 60% and 57%. The results did not differ significantly between vitrification and ultra-rapid freezing (p>0.05). Conclusion: As known in the above results, there were no significant differences in the fertilization and blastocyst formation rate of the frozen-thawed mouse mature oocytes by vitrification or ultra-rapid freezing among the control and treatments. It is suggested that vitrification and ultra-rapid freezing method were effective for the cryopreservation of mouse mature oocytes.

• International Journal of Highway Engineering
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• v.11 no.4
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• pp.143-152
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• 2009

The purpose of this study is to evaluate the freezing index and frost penetration depth. The freezing index and frost penetration depth were analyzed using air temperature and temperature profile of pavement system in Korea LTPP-SPS(Long Term Pavement Performance-Specific Pavement Study) site. The predicted frost penetration depth were then compared with the measured one from the LTPP sites. And the trend of annual freezing index was analyzed using the temperature data of meteorological stations located in the vicinity of Korea LTPP-SPS site. The result showed that the freezing index was rapidly decreased since 1987, and it was known that the use of freezing index determined from the past 30 years temperature data could cause the over estimates in the pavement thickness design. The temperature profile measured at 3 sections of LTPP-SPS sites showed that the temperature of subbase layer was above $0^{\circ}C$, even though anti-frost layers were found in these sections. Comparing the measured and calculated frost depth, the frost depth calculated from the subgrade frost penetration permissible method showed a similar trend with the measured frost depth.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.27 no.2
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• pp.95-101
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• 2013

This paper presents the comparison of the earth resistivity obtained from the measurements made with the three-electrode and four-electrode fall-of-potential techniques. The ${\rho}-a$ curve obtained from Wenner four-electrode method in undisturbed earth is in good agreement with the ${\rho}-l$ curve obtained from the three-electrode method based on the fall-of- potential method. However, The ${\rho}-a$ curve in disturbed earth with moisture and freezing is significantly different with the ${\rho}-l$ curve. The ${\rho}-a$ curve is considerably sensitive to the freezing and the moisture present in the earth surface compared to the ${\rho}-l$ curve. Thus to determine the actual earth resistivity, it is necessary to take into account the earth surface conditions when measuring the earth resistivity.

• Proceedings of the Korea Concrete Institute Conference
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• 2004.05a
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• pp.372-375
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• 2004

Scaling deterioration and resistance to freezing of port concrete structures due to the combined effects of chemical actions by containning chlorides and the freeze-thaw action is also a problem which has not yet been fundamentally solved. Furthermore, deterioration of concrete surface was considered as accelerate factor of concrete durability tended to decrease. Therefore, we considered the scaling measuring method and decreasing influence of durability of concrete according to kind of binders, such as OPC, Slag, Slag+Fa, due to freeze and thaw of concrete by containing chlorides. As a results of this study, it was effective method of scaling deterioration and resistance freewing of concrete, and confirmed the salt deterioration resistance effect to use slag binder against to containing chlorides.

• Journal of Korea Multimedia Society
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• v.21 no.12
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• pp.1387-1395
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• 2018

Core algorithm of deep learning Convolutional Neural Network(CNN) shows better performance than other machine learning algorithms. However, if there is not sufficient data, CNN can not achieve satisfactory performance even if the classifier is excellent. In this situation, it has been proven that the use of transfer learning can have a great effect. In this paper, we apply two transition learning methods(freezing, retraining) to three CNN models(ResNet-50, Inception-V3, DenseNet-121) and compare and analyze how the classification performance of CNN changes according to the methods. As a result of statistical significance test using various evaluation indicators, ResNet-50, Inception-V3, and DenseNet-121 differed by 1.18 times, 1.09 times, and 1.17 times, respectively. Based on this, we concluded that the retraining method may be more effective than the freezing method in case of transition learning in image classification problem.

• Journal of Embryo Transfer
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• v.18 no.1
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• pp.43-50
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• 2003

The objective of this study was to investigate the effect of cryopreservation by slow and rapid freezing on the sperm motility index, viability and morphology of post-thaw human spermatozoa. After rapid freezing and thawing, sperm motility index was significantly higher (MOT:47.40$\pm$20.06%, VCL : 38.12$\pm$15.58 $\mu$m/s, VSL : 28.19$\pm$14.10 $\mu$m/s, VAP:33.64$\pm$15.15 $\mu$m/s, and HYP 2.77$\pm$2.71%) than slow freezing and thawing(MOT : 43.39$\pm$ 18.79%, VCL .33.91 $\pm$ 13.50 Um/s, VSL . 19.98$\pm$0.88 $\mu$m/s, VAP : 24.60$\pm$11.72 $\mu$m/s, and HYP . 1.33$\pm$1.57% ; P<0.05). But sperm Linearity(LIN) was significantly lower(28.83 $\pm$ 10.35) comparing to the slow freezing method(34.64 $\pm$ 11.36 ; P<0.05). On the other hand, significant difference were not observed MAD, WOB, DNC and DNM by slow and rapid frozen-thawed methods. After rapid freezing and thawing, sperm viability was lower(60 $\pm$ 2.2%) than slow freezing method(62 $\pm$2.1%) and sperm morphology was higher(46$\pm$7.7%) than that(44: 8.3). But there was no significantly These results indicate that rapid freezing method was positive effect of sperm cryopreservation in human.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.17 no.11
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• pp.1088-1094
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• 2005

In the present paper, a new method is proposed for the measurement of the ice packing factor (IPF) of an ice slurry using the index of refraction. The purpose of the new method is to improve the resolution of the measurement and to increase its resistance to electric noise as compared to the standard IPF measurement technique that relies on measurement of the freezing temperature. These two methods are similar in that they both obtain a concentration of aqueous solution from measured physical quantities and calculate the IPF using a relation between concentration and IPF. We experimented and compared the two methods, whose results were also compared with results from the calorimeter method obtaining the IPF directly They are in good agreement (within $5\%$), which demonstrates the validity of the newly proposed method.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.19 no.8
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• pp.599-606
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• 2007

An experimental study was performed to measure a ice concentration of ice slurry flowing in a pipe in a real time. In the present paper, we suggested a measuring method by a refractive index and compared it to other methods by a freezing point and a density. To measure the refractive index of the solution, ice particles in the ice slurry should be completely removed and a hydro-cyclone was introduced instead of a mesh. The measuring method through the refractive index coincided with the density method using the real-time solution density within ${\pm}5%$ error range, having the error range less than the other two methods. In the other hand, the measuring method through the density has a good resolution, but the result using the initial density of the solution was different more than 10% error from that using the real-time density. And it has an error range 1.5 times greater than the method through the refractive index.

• Journal of Embryo Transfer
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• v.6 no.2
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• pp.30-36
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• 1991

The method of vitnilcation has various merits. It needs neither seeding nor slow freezing. It can freeze embryo by putting it directly into liquid nitrogen at the indoor temperature to $0^{\circ}C$. The operation process is quite easy. Moreover, higher promise of survival can be expected as there is no physical damage by any lumps of ice with the exception of cells. In Kasal's experiment (1990) using EFS liquid and Kang's experiment (1991) using GFS liquid the ratio of the damaged embryo was only 2-3%. But, the method of vitrification is now on the process of improvement, and the final or united method is not yet established. At the present time, most of the major institutes all over the world are using the traditional freezing method in the preservation of mouse embryo, but it is very likely that the vitrification will prevaIl in the near future considering the various merits of it. Calves can be begotten from the embryo by means of vitriilcated preservation in the cases of cow, rat, and rabbit as well as of mouse. In addition, recent experiments have shown that vitrificated preservation was successful in the case of drosophila embryo which was much bigger than mammalian embryo, which fact tells that this method is expected to be preferably used even in the preservation of living organs in the near future.

• 한국전자현미경학회:학술대회논문집
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• 2005.05a
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• pp.47-48
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• 2005