• 제목/요약/키워드: Freezing Method

검색결과 695건 처리시간 0.028초

Storage of Bull and Boar Semen: Novel Concepts Derived Using Magnetized Water and Antioxidants

  • Lee, Sang-Hee;Cheong, Hee-Tae;Yang, Boo-Keun;Park, Choon-Keun
    • Reproductive and Developmental Biology
    • /
    • 제38권1호
    • /
    • pp.1-8
    • /
    • 2014
  • Artificial insemination technique has been contributed immensely for production of livestock worldwide as a critical assisted reproductive technique to preserve and propagate excellent genes in domestic animal industry. In the past decade, methods for semen preservation have been improved mostly in liquid preservation method for boar semen and freezing method for bull semen. Among many factors affecting semen quality during preservation, reactive oxygen species, produced by aerobic respiration in sperm for survival and motility, are unfavorable to sperm physiology. In mammalian cell as well as in the sperm, antioxidant system plays a role in degradation of reactive oxygen species. Magnetized water forms smaller stabilizing water clusters, resulting in high absorption and permeability of the cell for water, implicating its application for semen preservation. Therefore, this review focuses on preservation methods of boar and bull semen with respect to improvement of extender and reduction of reactive oxygen species by using magnetized water and supplementation of antioxidants.

한우 수정란의 동결보존 및 쌍자생산에 관한 연구 III. 이분 체외수정란의 배양과 동결 (Studies on Embryo Cryopreservation and Twinning by Embryo Transfer of Korean Native Cattle: III. Culture and Freezing of IVF Bisected Embryos)

  • 손동수;김일화;이호준;양병철;최선호;이광원;노규진;최상용
    • 한국수정란이식학회지
    • /
    • 제12권2호
    • /
    • pp.151-159
    • /
    • 1997
  • In vitro fertilization(IVF) derived morula and blastocyst embryos were bisected by a simple method and cultured in vitro without zona pellucida And also bisected embryos were frozen-thawed and cultured in vitro) to evaluate the survival rate. The results obtained were as follows : The average number of grade I or II immature follicular oocytes recovered by slicing method per ovary was 11.9 from 142 ovaries. Following in vitro fertilization, the rates of cleavage and in vitro development to morula and blatocyst were 61.7 and 32.2% respectively. The successful bisection rate of IVE embryos was 67.51%, and the embryos of blastocyst stage were bisected successfully at significantly(P

  • PDF

Accelerated life testing of concrete based on stochastic approach and assessment

  • Zhu, Binrong;Qiao, Hongxia;Feng, Qiong;Lu, Chenggong
    • Computers and Concrete
    • /
    • 제19권1호
    • /
    • pp.111-120
    • /
    • 2017
  • This study aimed to design the accelerated life testing (ALT) of concrete, which stimulating the special natural environment maximumly. Its evaluation indexes, such as dynamic elastic modulus, mass and ultrasonic velocity were measured, and the variation of relative mass and relative dynamic elastic modulus of concrete were studied. Meanwhile, the microanalysis method was used. Moreover, an exploratory application of the stochastic approach, the Weibull distribution and the lognormal distribution, were made to assess the durability of concrete structures. The results show that the ALT for simulating natural environment is more close to the service process of concrete structure under actual conditions; The relative dynamic elastic modulus can be used as the dominant durability evaluation parameters, because it is more sensitive to the environmental factors compared with the relative quality evaluation parameters; In the course of the concrete deterioration, the destruction of the salt freezing cycle is the dominant factor, supplemented by other factors; Both of those two stochastic approaches can be used to evaluate the reliability of concrete specimens under the condition of ALT; By comparison, The lognormal distribution method is better to describe the reliability process.

Status and Prospect of Test Methods of Quality Silicone Water Repellent for Protecting Reinforced Concrete

  • Sun, H.Y.;Yuan, Z.Y.;Yang, Z.;Shan, G.L.;Shen, M.X.
    • Corrosion Science and Technology
    • /
    • 제16권3호
    • /
    • pp.141-150
    • /
    • 2017
  • Impregnating with quality silicone water repellent on the concrete surface is an effective method of protecting concrete. Quality silicone water repellent has been widely used in the engineering profession because of its desirable properties such as hydrophobicity, keeping concrete breathable and preserving the original appearance of the concrete. The companies in China that produce silicone water repellent are listed. Test methods in the specifications or standards about silicone water repellent in China are summed. The test methods relative to durability of concrete impregnated with silicone water repellent (such as resistant to chloride ion penetration, resistant to alkali, resistance to freezing and thawing and weatherability etc.) and the constructive quality (such as water absorption rate, impregnating depth and the dry velocity coefficient etc.) are compared and analyzed. The results indicate that there are differences among test methods relative to different specifications with the same index and therefore, confusion has ensued when selecting test methods. All test methods with the exception of the method of water absorption rate by using a Karsten flask are not non-destructive methods or conducted in a laboratory. Finally, further research on silicone water repellent during application is proposed.

일정 열유속 조건의 판형 히터에 의한 해빙과정의 수치해석 (Numerical Analysis of the Melting Process of Ice Using Plate Heaters with Constant Heat Flux)

  • 김학구;정시영;허남건;임태원;박용선
    • 설비공학논문집
    • /
    • 제19권6호
    • /
    • pp.434-440
    • /
    • 2007
  • One of the cold start problems of a FCV is the freezing of the water in the water tank when a FCV is not in operation and the surrounding temperature drops below $0^{\circ}C$. The ice in the tank should be melted as quickly as possible for a satisfactory operation of fuel cell vehicles. In this study, the melting process for the constant heat fluxes of the plate heaters was numerically calculated in the 2-D model of the tank and plate heaters. The enthalpy method and FVM code was used for this analysis. The changes of the temperature with heat fluxes and the heat transfer area could be investigated. The energy balance error was found to increase with the heat flux. From this numerical analysis, the proper heat flux value and some important design factors relating local overheating and pressurization of the water tank could be examined.

비틀림을 받는 사각주의 응력해석 (Stress Analysis of Rectangular Bar under Torsion)

  • 김동현;지중조;윤갑영
    • 한국정밀공학회지
    • /
    • 제3권4호
    • /
    • pp.53-63
    • /
    • 1986
  • In this study, the stress distribution of rectangular bar under torsion, when warping of both ends is free or constrained, is investigated. Method of separation of variable and Fourier Series are used for the theoretical analysis, and 3dimensional photoelastic stress-freezing method for experimental analysis. The main results are as follows; 1) In the case of warping-constrained rectangular bar, the normal stresses are negligible because they are less then 0.5% of the shear stresses. The maximum normal stress is placed on the point of y=0.61 b when b/a=1 and it gradually moves to the corner y=b when the value of b/a is increased. 2) According to increase of the value of b/a, on the crossection, the maximum shear stress is placed on the middle point of the long side (x=${\pm}a$, y=0) when warping of both ends is free but the middle of the short side (x=0, y=${\pm} b$) when warping is constrained. The stress distribution is straight line when warping is constrained, namely, the stress distribution is proportional to the distance from the axis of centroid, but parabolic when warping is free. 3) The values of the combined stress of warping-constrained bar, if the influence of the loaded point is neglected, are generally smaller than those of warping-free.

  • PDF

금산지역의 수삼의 저장관리 및 유통 현황 (Distribution Characteristics and Status of Fresh Ginseng in Keumsan Area)

  • 김현호;황용수;성봉재;김선익;조진웅;김충수
    • 농업과학연구
    • /
    • 제33권2호
    • /
    • pp.129-140
    • /
    • 2006
  • There are need to develop of merchandise of value added fresh ginseng because of high consciousness level of consumer and enlarge of markets for high quality products. The fresh ginseng after harvest was distributed to farmer partually but in general, it was to market by consigner or wholsaler directly after harvest. There were a high difference on storage period of fresh ginseng in different harvesting seasons. The reduction of value of commodities of fresh ginseng for storage period was caused by decomposition and tender of tissue. The storage temperature was under the freezing point and the packing method was sealing tightly by plastic film. As the quality of fresh ginseng was defined by naked eye, it was difficult to sort the quality of ginseng directly harvest.

  • PDF

영양 사암을 대상으로 한 에틸실리케이트 계열 처리제의 강화효과 평가 (Quantitative Evaluation for Effectiveness of Consolidation Treatment by Using the Chemical of Ethyl Silicate Series for the Sandstone in Yeongyang)

  • 이장존;한민수;송치영;전병규;도민환
    • 보존과학연구
    • /
    • 통권30호
    • /
    • pp.125-136
    • /
    • 2009
  • Stone cultural heritages in Korea have a severe damages from chemical and biological weathering because most of them have been situated in outdoors without any suitable protection systems, and this in turn causes deformation and structural damage. To counteract these problems and increase durability, various kinds of conservation materials are used in the conservation and restoration treatments. However until now there are not many practical and technological experiments on this subject. This paper attempts quantitative evaluation of effectiveness about chemical of ethylsilicate based resin for sandstone in Yeongyang-gun. It takes a long time to evaluate durability and side effect after conservation materials treatment. So we use artificial weathering through freezing§ thawing experimental method. As a result of this experiment, porosity and absorptance increased, and elastic wave speed, elastic modules, unconfined compression strength and tensile strength decreased more than before. This study plans to make a scientific method study about weathering factor and mechanism, and to deduce correlation between artificial weathering and natural weathering.

  • PDF

Immunological Assay to Detect Irradiated Beef

  • Lee, Ju-Woon;Yook, Hong-Sun;Lee, Hyun-Ja;Kim, Jung-Ok;Byun, Myung-Woo
    • Preventive Nutrition and Food Science
    • /
    • 제6권2호
    • /
    • pp.91-95
    • /
    • 2001
  • Competitive indirect enzyme linked immunosorbent assay (Ci-ELISA) was used to obtain the preliminary data for the detection of irradiated beef. Ci-ELISA was individually formatted with polyclonal antibodies produced from 2 kinds of bovine proteins, myosin and bovine serum albumin (BSA). Beef round, loin and tender loin were vacuum-packaged and subdivided into 3 groups of 1) irradiation; 2) irradiation and chilled at 4$^{\circ}C$ for 7 day; 3) irradiation and frozen at 2$0^{\circ}C$ for 2 months to observe the changes under different storage and/or distribution conditions. Irradiation was performed at 3, 5 and 7 kGy. Protein solutions prepared from the sample were tested by formatted Ci-ELISA. Detected concentrations of myosin and BSA decreased with the increased irradiation dose in all samples with different reduction rates. Myosin was more susceptible to freezing than BSA. Samples irradiated at 5 kGy or above could be differentiated from non-irradiated ones by Ci-ELISA. These results indicate that immunological assay can be used as a detection method for irradiated beef.

  • PDF

공기층 저항을 고려한 사각형 주형내에서의 2차원 상변화문제에 관한 연구 (A Study on the Two-Dimensional Phase Change Problem in a Rectangular Mold with Air-Gap Resistance to Heat Flow)

  • 여문수;손병진;김우승
    • 대한기계학회논문집
    • /
    • 제16권6호
    • /
    • pp.1205-1215
    • /
    • 1992
  • 본 연구에서는 1차원 상변화문제를 확장하여 사각형 주형내에서의 접촉열저항 을 고려한 2차원 상변화문제를 해석하고자 한다.즉 고상/액상 경계면인 상변화면을 추적하기 위하여 온도수식법(temperature formulation method)을 적용시 나타나는 복 잡성을 극복하기 위하여 제시된 엔탈피법을 이용하여 순수물질의 경우 뿐만 아니라 2상영역이 존재하는 합금에서의 온도분포, 상변화면의 이동속도 및 총응고에 소요되는 시간등의 열적특성을 해석하며, 또한 이러한 열적특성에 영향을 미치는 매개변수를 도출하고자 주형의 형상, 주형 외벽에서의 열전달계수, 접촉 열전달계수, 주형의 열전 도계수 및 주물의 초기온도등의 변화에 따른 효과를 고찰한다.