• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.27 no.2
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• pp.81-86
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• 2015

Performance of freezing and refrigeration systems in supermarket, which utilized $CO_2$ as a refrigerant, was investigated by using the Pack Calculation II. The configuration of simulated systems was basic parallel refrigeration system, cascade system, and two-stage system. The $CO_2$ cascade system showed higher COP than basic parallel R404A system by 13% for MT and 62% for LT, respectively. Among the $CO_2$ cascade systems, R717(MT)-$CO_2$(LT) showed the highest performance. Open-type intercooler method showed higher performance than liquid injection for the two-stage $CO_2$ systems.

• Proceedings of the Korea Association of Crystal Growth Conference
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• 1998.09a
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• pp.9-13
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• 1998

Large vacuum Bridgman-Stockbarger (BS) equipments was composed for growing large diameter CaF2 crystals. The CaF2 crystal of 4.5-inch was grown under the conditions of freezing temperature gradient of 12$^{\circ}C$/cm and growing rate of 3mm/hr. Also the 6-inch crystal was grown by using thermal stabilization method under freezing temperature gradient of 14$^{\circ}C$/cm and growing rate of 2mm/hr. The dislocation density was characterized for evaluating the quality of crystals. And the optical properties such as transmittance, refractive index and fluorescence were analyzed in order to investigate on the applications of optical components.

• Proceedings of the Korea Concrete Institute Conference
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• 2004.11a
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• pp.197-200
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• 2004

The increase of the use of recycled aggregate is necessary for the decrease of building waste, but it is not possible to use concrete because of the various range of quality. Therefore, the quality grade and application method of recycled aggregate should be prescribed before the site application so in that this study we investigated the effect of the quality of recycled aggregate on the compressive strength, resistance freezing and thawing. As results of this study, the compressive strength of recycled aggregate concrete used recycled aggregate, as if it had high quality recycled aggregate is similar or higher to that of crushed stone. Also, this high quality recycled aggregate affect the increase of resistance freezing and thawing.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2001.11a
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• pp.22-27
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• 2001

The purpose of this study is development of dry floor tile method that practically used for improving wet method's defect, with resin mat design. PE resin used mat which satisfied with bonding test, waterproof test, resistance to chemical attack test, resistance to impact test and freezing and thawing test is confirmed the basic property.

• Proceedings of the Korean Society of Crop Science Conference
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• 2000.04a
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• pp.88-89
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• 2000

• Proceedings of the Korean Ceranic Society Conference
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• 2000.04a
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• pp.50.1-50.1
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• 2000

• 제어로봇시스템학회:학술대회논문집
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• 1995.10a
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• pp.283-286
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• 1995

Due to finite bandwidth of missile dynamics, guidance commands in PN guidance tend to diverage as the missile approaches to the target. In this paper, a new method based on the short-time stability theorem is introduced to extend the stability region.

• Journal of Biomedical Engineering Research
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• v.34 no.4
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• pp.182-188
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• 2013

This study aims to develop a practical algorithm which can detect freezing of gait(FOG) in patients with Parkinson's disease(PD). Eighteen PD patients($68.8{\pm}11.1yrs.$) participated in this study, and three($68.7{\pm}4.0yrs.$) of them showed FOG. We suggested two time-domain algorithms(with 1-axis or 3-axes acceleration signals) and compared them with the frequency-domain algorithm in the literature. We measured the acceleration of left foot with a 3-axis accelerometer inserted at the insole of a shoe. In the time-domain method, the root-mean-square(RMS) acceleration was calculated in a moving window of 4s and FOG was defined as the periods during which RMS accelerations located within FOG range. The parameters in each algorithm were optimized for each subject using the simulated annealing method. The sensitivity and specificity were same, i.e., $89{\pm}8%$ for the time-domain method with 1-axis acceleration and were $91{\pm}7%$ and $90{\pm}8%$ for the time-domain method with 3-axes acceleration, respectively. Both performances were better in the time-domain methods than in the frequency-domain method although the results were statistically insignificant. The amount of calculation in the time-domain method was much smaller than in the frequency-domain method. Therefore it is expected that the suggested time domain algorithm would be advantageous in the systematic implementation of FOG detection.

• Journal of Embryo Transfer
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• v.29 no.3
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• pp.235-240
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• 2014

Ethylene glycol (EG) has been successfully used as a cryoprotectant for vitrification of mammalian embryos (including human embryos) due to its low formula weight and high permeation into cells compared with other cryoprotectants, including propylene glycol (PROH). Cryopreservation is able to store the surplus pre-embryos for freezing and furthermore thawing and transfer in a subsequent cycle. This study was carried out to evaluate the effects of embryonic stage, cryoprotectant, and freezing-thawing method on the rates of survival and development of the cryopreserved mouse early embryo and finally to establish the cryopreservation method of surplus embryos obtained during assisted reproductive technology (ART). Female ICR mice (6~8 weeks old) were induced to superovulate by sequential intraperitoneal injection of 5 IU PMSG and 5 IU hCG 48 h apart. Mouse embryos were collected according to its developmental stage after the injection of hCG. Embryos were cryopreserved not only during cryoprotectant step (1~4 step) but also in a variety of media (HTF, IVF medium, D-PBS) and cell stage. The results were as follows : There is no clear advantage in these freezing media of rapid method, but 4 cell and 8 cell of slow method (2, 3 and 4 step) have advantage in D-PBS. The development of embryos according to cell stage become greater in 8 cell stage. In the treatment steps of cryopreservation, the development of embryo to blastocyst was similar among rapid method, but the development of 4 cell and 8 cell embryos to blastocyst according to slow method was better than rapid method.

• Journal of Microbiology
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• v.45 no.6
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• pp.593-596
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• 2007

In this study, we describe the development of a simple and efficient method for cell lysis via the insertion of a bacteriophage lambda lysis gene cluster into the pET22b expression vector in the following order; the T7 promoter, a gene for a target protein intended for production, Sam7 and R. This insertion of R and Sam7 into pET22b exerted no detrimental effects on cellular growth or the production of a target protein. The induction of the T7 promoter did not in itself result in the autolysis of cells in culture but the harvested cells were readily broken by freezing and thawing. We compared the efficiency of the cell lysis technique by freezing and thawing to that observed with sonication, and determined that both methods completely disintegrated the cells and released proteins into the solution. With our modification of pET22b, the lysis of cells became quite simple, efficient, and reliable. This strategy may prove useful for a broad variety of applications, particularly in experiments requiring extensive cell breakage, including library screening and culture condition exploration, in addition to protein purification.