• Proceedings of the Korea Concrete Institute Conference
• /
• 2008.04a
• /
• pp.161-164
• /
• 2008

In recent years, carbon fiber-reinforced polymer (CFRP) has been widely used for repairing and/or strengthening structural elements in concrete. Not enough test data, however, are available to predict the long-term performance of the repaired and improved structures exposed to weathering. The objective of this research is to study the effect of freeze-thaw cycling on the behavior of reinforced concrete (RC) beams strengthened in shear with carbon fiber sheet. Six small-scale RC beams (100mm${\times]$100mm${\times]$400mm) were strengthened with CFRP in shear, subjected to up to 400 cycles freeze-thawing from -17${\sim}4^{\circ}C$, and tested to failure in four-point bending. Test result, there was no significant damage to carbon fiber sheet strengthened concrete beams had been suffered 30 cycles of freeze-thawing, and more over 60 cycles of freezing-thawing brought about a reduction in resistance of only 25% of the initial level.

• Microbiology and Biotechnology Letters
• /
• v.49 no.2
• /
• pp.157-166
• /
• 2021

The probiotic market is constantly continuing to grow, concomitantly with a widening in the range and diversity of probiotic products. Probiotics are defined as live microorganisms that provide a benefit to the host when consumed at a proper dose; the viability of a probiotic is therefore of crucial importance for its efficacy. Many products undergo lyophilization for maintaining their shelf-life. Unfortunately, this procedure may damage the integrity of the cells due to stress conditions during both the freezing and (vacuum-) drying process, thereby impacting their functionality. We propose a lysine-based mixture for rehydration of freeze-dried probiotics for improving their viability during in vitro simulated gastric and duodenum stress conditions. Measurement of the zeta potential served as an indicator of cell integrity and efficacy of this mixture, while functionality was estimated by adhesion to a human enterocyte-like Caco-2 cell-line. The freeze-dried bacteria exhibited a significantly different zeta potential compared to fresh cultures; however, this condition could be restored by rehydration with the lysine mixture. Recovery of the surface charge was found to influence adhesion ability to the Caco-2 cell-line. The optimum lysine concentration of the formulation, designated "Zeta-bio", was found to be 0.03 M for improving the viability of Lactiplantibacillus plantarum Lp-115 by up to 13.86% and a 7-strain mixture (400B) to 41.99% compared to the control rehydrated with distilled water. In addition, the lysine Zeta-bio formulation notably increased the adherence ability of lyophilized Lp-115 to the Caco-2 cell-line after subjected to the in vitro stress conditions of the simulated gastrointestinal tract passage.

• Journal of the Korean Geotechnical Society
• /
• v.25 no.9
• /
• pp.19-27
• /
• 2009

In order to investigate anti-freeze process of precast concrete L-type retaining walls in cold regions, test walls were installed in the campus of Kitami Institute of Technology (KIT, Hokkaido, Japan). The wall consists of following three sections, i) back filled with frost susceptible clay soil, ii) using thermal insulation material, and back filled with frost susceptible soil, iii) back filled with frost-unsusceptible soil. The freezing front distribution and ground temperature within the backfill were observed and deflections of the walls were measured over three freeze-thaw seasons. Some understanding of the mechanisms of the build-up of frost heave pressure was gained, and the effectiveness of replacement method was observed. A simulation was performed to predict the shape of the freezing front in the backfill behind L-type walls with various cross sections. These findings were employed to propose a method for determining the appropriate zone to be replaced with frost unsusceptible backfill material in cold regions.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.23 no.6
• /
• pp.406-412
• /
• 2011

This paper describes the analysis on freeze and refrigeration capacity of cold storage facilities for fisheries products during the last 15 years. And based on the analysis results, we prospect the number and scale of cold storage facilities in the near future. The main analysis results are summarized by the followings; Under the present conditions, the number and scale of our facilities is 879 and 3,586,824 ton, respectively. In comparison of the present and last 15 years ago, the business number increases about 22%, the accommodation capacity increases approximately 80%. In the reported update reference with the related data during the last 15 years, the capacity of cold storage facilities in 2011 expects to reach up to 4,600,000 ton. The newlyestablished facility in 2011 has the function of a multi-purpose distribution center as well as the existing cold storage, but this facilities converges in the capital area. Therefore, in this paper, we provided the basic reference data for the present situation and prediction of our cold storage facilities. But it is necessary to investigate and analyse widely our cold storage facilities for fisheries products in the near future.

• Proceedings of the Korea Concrete Institute Conference
• /
• 2009.05a
• /
• pp.21-22
• /
• 2009

This paper presents the results of an experimental study on flexural behavior and cracking process of concrete beams subjected to cycles of freezing and thawing after patch-repaired with strain-hardening cement composites (SHCCs). The SHCCs were reinforced with hybrid 0.75% PVA and 0.75% PE fibers. Experimental testing of concrete beams patch-repaired with SHCCs revealed that the SHCC patch-repair system without freeze thaw (FT) exposure showed average 3.31 times increased load carrying capacity and for beams exposed to 300 FT cycles, load carrying capacity increased up to 2.42 times. Cracking damage of SHCC patch-repaired beams mitigated compared to plain concrete beams but this trend decreased under FT exposure.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2002.11b
• /
• pp.53-53
• /
• 2002

Shoot tips of in vitro propagated plantlets were cryopreserved using encapsulation/dehydration procedures. Shoot tips were excised under filter sterilized antioxidants solution (0.2M phosphate buffer, pH 5.7 supplemented with 5g/1 ascorbic acid and 15g/1 sodium borate). They were drawn up into a sterile 10 $\textrm{cm}^3$disposable pipette and were dropped into the culture medium with 2.5w/v Na-alginate, then into 100mM CaCl$_2$.2$H_2O$. Encapsulated shoot tips were transferred into 10㎤ of liquid culture medium with a range of sucrose concentrations (0.25-1.0M) and were incubated in dark for 24 hours in 18C at 40rpm. Beads were then dehydrated in silica gel for different time intervals (1-24 hours). Then they were freeze dried either rapidly (plunge directly into liquid N2 or in two stages (samples were kept at 20C for 10 minutes, then reduced to 35C at 1C per minute. Then, plunge into liquid $N_2$). The influence of sucrose and silica gel pre-treatment on pre- and post-freeze shoot growth was examined.(중략)

• Journal of the Society of Disaster Information
• /
• v.12 no.1
• /
• pp.98-104
• /
• 2016

This study clarifies the simulation on freezing of remaining water in the Z-trap set up in the PVC sewage bay to control malodor. It made use of commercial FEM LAB program(ver. 3.2) well known as a solution of the problems arising in the flow of various fluid, heat transfer and mass transfer. Simulation results under the temperature $-20^{\circ}C$ outwards show that the water in the Z-trap set up in the sewage bay to control malodor freeze in the 60cm under the ground level after 14 days in the wet ground, and after 17 days in the regular ground. On the other hand, if the soil is dry even after the 42 days does not go down below freezing. Therefore, the water in the Z-trap was confirmed that it does not freeze.

• International Journal of Highway Engineering
• /
• v.11 no.3
• /
• pp.51-60
• /
• 2009

This study investigates the fresh state characteristics, strength, chloride ion penetration resistance and freeze-thaw resistance of the suggested high strength-high durability cement concrete pavement. The required workability and air content could be achieved by using an appropriate admixtures. However its dosage should be carefully determined through field trial batches. Compressive strength increased with the increased cement content and, in particular, high cement volume concrete continuously developed strength up to 90 days. No clear relationship, however, existed between flexural strength and cement content. Chloride penetration resistance seemed as a function of curing age rather than the cement content. Freeze-thaw resistance test was conducted using two different coolants, tap water and 4% NaCl solution. When the tap water was used no severe damage was observed up to 300 cycles regardless the air content. Under 4% NaCl solution, specimens of 326kg/$m^3$ cement content showed severe damage with surface scaling. Based on the experimental investigations herein, it is highly recommended that the cement content be greater than 400kg/$m^3$ for strength-high durability cement concrete pavement structures.

• The Korean Journal of Nuclear Medicine Technology
• /
• v.16 no.2
• /
• pp.139-148
• /
• 2012

Purpose : Since standard solution is the one that knows its exact concentration, the curve of the dissolution has been determined according to the amount of the solution, compared to the amount of the unknown sample. Therefore, the antigen that makes up standard materials should be made in a pure form. The configuration of the standard substance solution in the kit we use is a freeze-dried material, or made and comes as a liquid. Lyophilized reference material is used after dissolving in usually D.W. (Distilled Water), and if the antigen to use is too sensitive, reagents should be freeze-dried. Furthermore, when freeze-dried reference has to be frozen again after being dissolved, it should be kept under $-20^{\circ}C$ until the expiration date according to the reports. Since it is not expressed in the experiment if it is safe or stable to reuse the solution which was dissolved a few times, thus, this time it is tested and evaluated that the changes of the standard solution by freezing and melting several times, and its results and the effectiveness of it were compared to the solution which was kept in a fridge. Materials and Methods : Among Vitro diagnostic kits on the market made by radioimmunoassay, parathyroid hormone (PTH), adrenocorticotropic hormone (ACTH), luteinizing hormone (LH) are made of freeze-dried standard solution and all composed of the same Lot.NO. These hormones melted in D.W. and were separated into three groups. In the first group, melting and freezing were repeated, and in the second group, The solution only for one time use was put into a test tube after melting and freeze it. The third group was kept in the refrigerator. This experiment has been conducted from January to February in 2012. January to 2012. PH test was employed because ph is prone to changing depending on the change of protein. Each group of the standard solution, cpm (counter per minute), and the patient relative concentration values were compared by date, and Through the correlation coefficient and Paired t-test, the significant level of each group was analyzed. Results : ACTH, PTH, LH pH values were too subtle denaturation rather than numerical changes in the protein. In addition, when the standard solution of ACTH, PTH, LH was refrigerated, after 3 days and 7 days, there was a significant difference observed between the solution being kept in a refrigerator and a freezer within a significance level. Conclusion : Standard solution should be kept in a freezer, and being kept in a fridge, it is recommended to use the solution as soon as possible.

• Korean journal of food and cookery science
• /
• v.17 no.5
• /
• pp.483-489
• /
• 2001

The objective of this study was to investigate the effect of ripening on the quality of kimchi or freeze-dried/rehydrated kimchi. Kimchi stored/ripened at 0$\^{C}$ for 120 days was taken at an interval of 10 days and freeze-dried/rehydrated with water. The number of lactic acid bacteria(LAB), shearing force and sensory properties of kimchi ripened (Raw sample) or kimchi ripened/freeze-dried/rehydrated (F/D sample) were observed. The results were as follows: (1) The number of LAB of Raw and F/D samples decreased slightly during ripening for 120 days, while the pH of both samples decreased. The number of LAB in kimchi decreased up to 6∼16% of the original value by freeze-drying. (2) Ripening for 120 days did not affect shearing force of kimchi while freeze-drying increased markedly its shearing force. (3) Although overall acceptability and taste of Raw and F/D samples decreased gradually, the degree of change between two samples was different. The odor of Raw and F/D samples decreased slightly during ripening for 120 days while the degree of change between two samples was similar after 70 days of ripening. Moistness of Raw and F/D samples was maintained relatively stable during ripening. Chewiness of Raw sample decreased during ripening for 120 days while that of F/D sample was not affected by ripening. Color of Raw and F/D sample was changed during ripening while the degree of change between two samples was not significant. It can be concluded that the change of some sensory properties between Raw sample and F/D sample was affected by ripening for 120 days while the change in the number of LAB, pH and shearing force between both samples was not affected markedly by ripening.