• Title/Summary/Keyword: Freeze-up

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Changes in Quality of Welsh Onion (Allium fistulosum L.) during the Freezing Storage Period under Different Freezing Conditions (냉동조건에 따른 대파의 냉동저장 중 품질변화)

  • Kim, Seok-Young;Kim, Hee-Sun;Kim, Jin-Se;Han, Gwi-Jung
    • Korean journal of food and cookery science
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    • v.32 no.6
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    • pp.665-676
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    • 2016
  • Purpose: This study was conducted to find the optimal freezing method and storage conditions for welsh onion. Methods: Cut welsh onions (0.3 cm) were packed in nylon/linear low density polyethylene (LLDPE) film bags, and frozen utilizing still-air freezing at -$20^{\circ}C$ (SAF20) and -$40^{\circ}C$ (SAF40), and immersed-liquid freezing at -$40^{\circ}C$ (ILF40); they were then stored at -$20^{\circ}C$ for 7 months. During storage, quality characteristics were measured monthly. Results: Drip loss was the lowest in the ILF40 packaging. Color difference in the stem (white part) did not differ significantly according to freezing conditions and storage time. Color difference in the leaf (green part) and stem was the lowest in SAF20. pH remained unchanged, while total aerobic bacterial count, pyruvic acid and moisture content decreased during storage. Pyruvic acid content of ILF40 was the highest among the freezing treatments. Fructose and glucose contents increased gradually during storage. Citric acid, malic acid, succinic acid and fumaric acid contents were unaffected, regardless of the freezing conditions. Conclusion: The optimal freezing method for welsh onions with the least quality changes was determined to be immersed liquid freezing, following by preservation up to 7 months by freeze-storing.

Partial Purification of Fig Pectinesterase and Characterization of its in situ Activity (무화과 펙틴에스테라제의 부분 정제 및 in situ 상태에서의 활성 특성)

  • Hou, Won-Nyoung;Kim, Myoung-Hwa;Go, Eun-Kyoung
    • Korean Journal of Food Science and Technology
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    • v.30 no.5
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    • pp.1169-1178
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    • 1998
  • This study was performed to purify fig pectinesterase(F-PE) and characterize its in situ activity. Three kinds of F-PE were partially separated by using ammonium sulfate fractionation, Q-Sepharose column, CM-cation exchanger column chromatography, and HPLC. One of those was anionic protein and the others were cationic proteins. All of them had approximate molecular weight of 27,000 and lost rapidly their activity during storage. Therefore alternative crude enzyme was prepared by suspending the freeze dried and milled fig powder in 0.1 M NaCl at pH 7.5. F-PE had the optimum pH of 8.5, the optimum temperature of $50^{\circ}C$ with activation energy of 7,671 cal $mol^{-1}K^{-1}$ and stability up to $55^{\circ}C$ with 10 minutes heating. Optimum activity was obtained in $0.2{\sim}0.4$ M NaCl with optimum solubility at above 0.8 M NaCl.

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Housewives' Awareness of the Quality Attributes for Korean Native Chickens (토종닭 품질 특성에 대한 주부의 인식 조사)

  • Kim, Hyun-Cheol;Lee, Min-A;Jo, Cheorun;Nam, Ki-Chang
    • Korean Journal of Poultry Science
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    • v.42 no.4
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    • pp.275-283
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    • 2015
  • A consumer survey was conducted for the subject of a total of 503 housewives in Korea, with the questionnaires on the quality attributes for Korean native chicken. The responding results collected were used for the importance-performance analysis of the quality attributes involved in production, processing, distribution, and consumption of Korean native chicken. The quality attributes with both relatively high importance and performance ("keep up the good work") were chicken origin, shelf life, freshness, and safety issues, while those with high importance but low performance ("concentrate here") were chicken breed certification and refrigerated/ frozen state. Respondents were also interested in lean meat and protein content of the chicken meat. In the aspect of eating quality attributes, chewiness was the most important one. The survey shows tenderer, chewier, and more flavorful chicken meat will be preferred and a new chicken breed with those eating quality attributes needs to be developed.

Cryo-SEM Methodology of Arabidopsis thaliana Stem Using High-Pressure Freezing (고압동결고정을 이용한 애기장대 줄기의 cryo-SEM 분석법)

  • Choi, Yun-Joung;Lee, Kyung-Hwan;Je, A-Reum;Chae, Hee-Su;Jang, Ji-Hoon;Lee, Eun-Ji;Kweon, Hee-Seok
    • Applied Microscopy
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    • v.42 no.2
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    • pp.111-114
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    • 2012
  • The scanning electron microscopy is an ideal technique for examining plant surface at high resolution. Most hydrate samples, however, must be fix and dehydrate for observation in the scanning electron microscope. Because the microscopes operate under high vacuum, most specimens, especially biological samples, cannot withstand water removal by the vacuum system without morphological distortion. Cryo-techniques can observe in their original morphology and structure without various artifacts from conventional sample preparation. Rapid cooling is the method of choice for preparing plant samples for scanning electron microscopy in a defined physiological state. As one of cryo-technique, high-pressure freezing allows for fixation of native non-pretreated samples up to $200{\mu}M$ thick and 2 mm wide with minimal or no ice crystal damage for the freezing procedure. In this study, we could design to optimize structural preservation and imaging by comparing cryo-SEM and convention SEM preparation, and observe a fine, well preserved Arabidopsis stem's inner ultrastructure using HPF and cryo-SEM. These results would suggest a useful method of cryo-preparation and cryo-SEM for plant tissues, especially intratubule and vacuole rich structure.

Preparation and In Vitro Test of Sold Dispersion using Acyclovir and Water Soluble Polymer (아시클로비어와 수용성 고분자를 이용한 고체분산체 제조 및 생체외 방출)

  • Ahn, Yong-San;Lee, Ha-Young;Hong, Keum-Duck;Jung, Sung-Beum;Cho, Sun-Hang;Rhee, John-Moon;Lee, Hai-Bang;Khang, Gil-Son
    • Journal of Pharmaceutical Investigation
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    • v.34 no.3
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    • pp.169-176
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    • 2004
  • Acyclovir(ACV) is an important antiviral drug used extensively against infections caused by herpes viruses, especially herpes simplex and varicella zoster. Because of high crystallinity and large particle size, solubility of intact ACV is very low in water(1.3 mg/ml). The goal of this work is to enhance the solubility of ACV. To make solid dispersion, Polyethyleneglycol, Hydroxyprophylmethylcelluose and Polyvinylpyrrolidone were used as polymer carriers in this work. Polymer carriers and drug were dissolved in acetic acid. And then spray drying method and freeze drying method were used as solvent extraction. Morphology, crystallization and functional group were characterized using SEM, XRD and FT-IR. The result of in vitro test showed the sample using PVP as polymer carrier had higher dissolution rate(up to 466%) than intact ACV.

Retrospective Clinical Study on Marginal Bone Loss of Implants with Guided Bone Regeneration (골유도재생술과 동시에 식립한 임플란트의 변연골 흡수량에 대한 후향적 고찰)

  • Park, Seul-Ji;Seon, Hwa-Gyeong;Koh, Se-Wook;Chee, Young-Deok
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.34 no.6
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    • pp.440-448
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    • 2012
  • Purpose: The purpose of this study was to evaluate marginal bone loss of the alveolar crest on implants with or without guided bone regeneration and variables that have influenced. Methods: The clinical evaluation were performed for survival rate and marginal bone loss of 161 endosseous implants installed with guided bone regeneration (GBR) in 83 patients from September 2009 to October 2010 in relation to sex and age of patients, position of implant, implant system, length and diameter of implant. Study group (n=42) implant with GBR procedure, control group (n=41) implant without GBR technique. Simultaneous GBR approach using resorbable membranes combined with autogenous bone graft or freeze-dried bone allograft or combination. Radiographic examinations were conducted at healing abutment connection and latest visit. Marginal bone level was measured. Results: Mean marginal bone loss was 0.73 mm in study group, 0.63 mm in control group. Implants in maxillary anterior area (1.21 mm) were statistically significant in study group (P<0.05), maxillary posterior area (0.81 mm) in control group (P<0.05). Mean marginal bone loss 1.47 mm for implants with diameter 3.4 mm, 0.83 mm for implants of control group with diameter 4.0 mm (P<0.05). Some graft materials showed an increased marginal bone loss but no statistically significant influence of sex, implant type or length. Conclusion: According to these findings, this study demonstrated the amount of marginal bone loss around implant has maintained a relative stable during follow-up periods. We conclude that implants with GBR had similar survival rate and crestal bone level compared with implants in native bone.

Quality Characteristics of Sulgidduk added with Barley Sprout using Different Drying Methods (건조방법에 따른 새싹보리 설기떡의 품질특성)

  • Lim, Young-Sook;Kim, Min-Ji;Kang, Yang-Sun
    • Culinary science and hospitality research
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    • v.23 no.7
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    • pp.220-233
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    • 2017
  • The purposes of this study were: (1) to examine the quality characteristics of the Sulgidducks added with barley sprouts by blanching (CON), freeze-drying (FBP), hot-air drying(HBP); (2) to examine the quality characteristics of Sulgidduck such as the moisture content, the color value, the textural properties and the sensory evaluation; (3) to find the optimal ratio of barley sprouts, which were made by the three different drying methods. As a result, the Sulgidduks containing barley sprouts by CON showed a lot of significance and high correlation depending on the amounts of the barley sprout(p<0.05). As the amounts of CON, FBP and HBP increased, the L and the a values of Sulgidducks significantly decreased, whereas the b values of them increased. For the textual properties of the Sulgidducks, on the first day, the concentration in CON up to 6% showed no significant differences in the hardness, cohesiveness, springiness, and chewiness. In case of FBP, the increase in the additives caused decrease in the hardness, adhesiveness, springiness, and chewiness of the Sulgidducks on storage 0 day(p<0.05). As the amounts of HBP increased, the hardness, springiness of the Sulgidducks on the first day decreased(p<0.05). Conclusionally, 6% blanched barley sprout added group, 2% FBP and HBP were highly rated in color, flavor, bitterness, chewiness, overall acceptability and all sensory quality characteristics.

Estimation of polycyclic aromatic hydrocarbons emission from sewage sludge of sewerage treatment plants in Korea

  • Kim, Dong-Hwan;Hwang, Jong-Seob;Lee, Min-Hee;Ok, Gon
    • Journal of Environmental Science International
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    • v.12 no.11
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    • pp.1167-1171
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    • 2003
  • Polycyclic aromatic hydrocarbons (PAHs) are an important group of organic contaminants present in sewage sludge, due to their persistence, toxic, bioaccumulative and long range transfer. These characters make themselves as Persistent Organic Pollutants(POPs) in Long Range Transboundary Air Pollutions convention(LRTAP) of Europe. A method of the gas chromatographicmass spectrometric (GCMS) determination of PAHs present in sewage sludge was developed and applied to analyzed samples from five sewerage treatment plants (SWTPs), having different treatment types. PAHs were extracted from freeze-dried samples by toluene 16 hours in a soxhlet extraction system. The sludge extracts were cleaned-up by an activated silica gel column chromatography. The sum of the 16 US Environmental Protection Agency PAHs sewage sludge samples varied from 2.44 to 4.82 $\mu\textrm{g}$/g. Concentration of emission carcinogen PAHs(PAHcarc), such as Benzo(a)anthracene, Benzo(b)f1uoranthene, Benzo(k)f1uoranthene, Benzo(a)pyrene, Dibenzo(a,h)anthracene and Indeno( 1,2,3-cd)pyrene ranged from 0.62 to 1.03 $\mu\textrm{g}$/g. The total amount of PAHs emission from sewage sludge in Korea was calculated as a top-down approach. PAHs and $\Sigma$PAHcarc from sewage treatment plants had emitted to environment by several pathway. The highest amount of emission to the environment was remarked in the ocean dumping(PAHs and $\Sigma$PAHcarc emissions were 1155.95 kg/year and 5040.32 kg/year, respectively).

Mechanical Properties and Durability of Concrete in Relation to the Amount of Limestone Use (석회석 혼입량에 따른 콘크리트의 역학적 및 내구특성)

  • Oh, Sungwoo;Shin, Dongcheol
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.21 no.3
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    • pp.138-144
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    • 2017
  • In order to reduce carbon dioxide emission in construction industry, less amount of cement use can be one of the alternatives to manufacture concrete. One of the non-sintered construction materials are limestone, which is the raw material to manufacture ordinary Portland cement(OPC). A large amount of limestone have already been used as binders such as blended cement in Europe and US. Even European countries were already established the standard of blended cement, where the limestone can be used up to 35 percent. In this study, experimental researches were conducted to investigate the effects of limestone replacement on the mechanical properties and durability of concrete with 15%, 25% and 35% of limestone substitution to use limestone in blended cement. 15 percent use of limestone in blended cement developed equivalent or even higher compressive strengths compared to Plain mixture. Porosity of limestone cement with 15 percent substitution was much lower than Plain mixture. Most durability tests such as concrete carbonation, freeze-thaw cycle and drying shrinkage strains were conducted to evaluate long-term performance, and the test results indicated that 15 percent of limestone use did not significantly influence on the concrete durability compared with plain concrete.

Processes of Thufur Disintegration Mt. Halla (한라산 유상 구조토의 붕괴 프로세스와 요인)

  • Kim, Tae-Ho
    • Journal of the Korean association of regional geographers
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    • v.12 no.4
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    • pp.437-448
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    • 2006
  • Bare-topped thufur is called as frost scars, implying the rupturing stage of vegetated mounds, Field observations and measurements provide empirical information on the process and factors of thufur disintegration in Mt. Halla, Initial bare patches on mound apexes are scaled up by continuous removal of soil and vegetation, resulting in the formation of crater-like thufur, Frost action plays a primary role in thufur disintegration, In particular, pipkrake loosens soil particles within the bare patches and subsequently accelerates the degradation of vegetated mounds during periods with frequent diurnal freeze-thaw cycle and high soil moisture, Deflation also has an impact on thufur breakup in that the bare patches usually lack upper dark brown soil and are covered with granules, Withered shrubs such as Juniperus chinensis var. sargentii are frequently observed in frost scars and disrupted mounds, indicating that thufur disintegration has been probably influenced by global warming.

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