• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.2
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• pp.182-188
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• 1997

The study was carried out to determine an optimum concentration of plant growth regulators(ABA and kinetin) on reducing NaCl toxicity during germination in rice. Plant height of a japonica cultivar Ilpumbyeo in ABA 10$^{-5}$ M was increased, while all treatments of kinetin had no clear effects on increasing the plant height. However, other rice cultivars showed slightly different reaction by PGRs compared with Ilpumbyeo. Germination rate of four rice cultivars was ranged from 53.1 to 58.2％ in NaCl 1.3％. All treatments of kinetin accelerated germination rate of tested rice cultivars except Dasanbyeo compared with NaCl 1.3％ treatment. The higher concentration of ABA and kinetin treatment induced the higher starch content, and while the lower concentration of two plant growth regulators induced the higher sugar content in all four rice cultivars. The free proline content of rice seedlings was highest in Ilpumbyeo among the tested cultivars under NaCl 1.3％ treatment.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.2
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• pp.189-195
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• 1997

To cope with increasing importance of water stress in food crop production, characteristics and cultivar differences in physiological responses of winter barley plants to water stress during seedling stage were studied employing three covered-barley, Milyang 12, Durubori, and Olbori, one naked-barley, Baekdong, and one two-row beer-barley, Hyangmaeg. Water stress was given to barley plants by withholding watering in soil-culture pots or by adding P.E.G. (polyethylene glycol, M. W. 4,000) to the nutrient culture solution. When water stress was imposed by withholding watering for 10 days to the seedlings grown in pot-soil for 10 days after emergence, leaf water potential of 5 cultivars was decreased almost to the same degree, from control -3.5 bar to the water stressed -26.6 bar. However, growth and some physiological responses were differently affected by cultivars: The plant height was decreased by 16-26％ : seedling dry weight by 25∼42％ ; chlorophyll content by 21∼39％ ; second leaf survival rate by 8∼18％ ; and free proline content per gram of leaf dry weight was increased from control 0.2∼0.5mg to the water stressed 5.8∼13.2mg. Drought resistances of 5 barley cultivars, based on the leaf survival rate and the decreased rate in seedling dry weight at the end of stress treatment, were high in the order of 3 covered-barley＞naked-barley＞two-row barley.

• Korean Journal of Food Science and Technology
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• v.5 no.2
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• pp.71-77
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• 1973

The results, which was analytically surveyed the free amino acids by the automatic amino acid analyzer adding the enzyme on the Korean cow's fore shank muscles, are as follows: 1. The content of free amino acids in the fore shank muscles, without addition of the enzyme orderly contains alanine, glutamic acid, lysine, glycine, histidine, leucine, threonine, arginine, cystine, serine, proline, isoleucine, phenylalanine, tyrosine, methionine, aspartic acid and valine. 2. In accordance with the addition of the enzyme, by 0.01%, 0.05% and 0.1% the nine free amino acids of glutamic acid, glycine, alanine, cystine, valine, isoleucine, leucine, lysine and arginine were continuosly increased. 3. Proline and histidine were decreased at the enzyme addition of 0.01% after showing the high content at the control, but the quantity of free amino acids was increased according to the increase of the quantity of the enzyme. 4. Aspartic acid, threonine, serine, methionine, tyrosine and phenylalanine were increased till the enzyme addition of 0.05% and remarkably decreased from 0.1%. 5. At cooking the meat, the quantity of the enzyme addition was most effective at 0.05% of meat weight.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2002.05b
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• pp.468-471
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• 2002

The present study was undertaken to Investigate the effects of low temperature and salicylic acid (SA) on chilling tolerance In acclimated and nonacclimated cucumber seedlings. Acclimation significantly affected survival and shoot dry weights. Injuries of acclimated seedlings at the third leaf stage were on the average smaller by half than those of the nonacclimated ones. Chilling caused a large increase in free proline levels, regardless of acclimation status. Exogenous treatment with SA resulted in improvement in growth and survival of acclimated, chilled seedlings, indicating SA and acclimation have common effects. Cycloheximide treatment In the presence of SA restored acclimation-induced chilling tolerance. An elevated proline level was observed in cold-treated and SA- treated plants and the level was more pronounced in the light than in the dark at chilled temperature, indicating that endogenous proline may play a role in chilling tolerance.

• Journal of Plant Biotechnology
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• v.5 no.1
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• pp.43-49
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• 2003

Resistant cell lines to azetidine-2-carboxylic acid (AZCA) were selected through rice embryo culture after mutagenic treatment of callus irradiated with 30,50,70,90 and 120 Gy. The optimum AZCA concentration for the selection of resistant cell lines was 3 or 4 mM AZCA considering $LD_{50}$ and the fresh weight of callus. Survival rate of the AZCA resistant callus showed remarkable increase in the callus irradiated with 50 and 70 Gy. Regeneration rate of the AZCA resistant callus was much lower on the whole. Ninety and 120 Gy increased the regeneration rate for calli selected from 3 and 4 mM AZCA, respectively. Based on fresh weight, survival rate and regeneration for selection of the AZCA resistant cell line, 50-90 Gy was considered as the optimum range of gamma irradiation. Irradiated calli selected from AZCA were more tolerant to NaCl than those from non-irradiated calli. It suggests that elevated resistance to osmotic stress resulted from mutagenic treatment. The level of free proline content in the AZCA resistant cell line was increased up to 3.5 times compared with that in the control. Proline content in the regenerant derived from the AZCA resistant cell line also increased to 1.7 times that from the control plants regenerated from callus grown in AZCA free medium. Selection of proline overproducing cell lines by in vitro mutagenesis was successful and seems to be useful for improvement of stress tolerance in this crop.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.3
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• pp.340-350
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• 1995

This study was conducted to obtain the basic information for the salt stress of Italian Ryegrass. Fourteen cultivars including 7 diploid and 7 tetraploid were subjected to eight levels of NaCl treatments during germination and early seedling stages. Germination percentage decreased remarkably over 300mM of NaCl concentration. The NaCl concentration that inhibited germination of 50% of the viable seeds in the cultivars was 344mM. The decreasing degree of germination percentage was higher in tetraploid type than in diploid type. The varietal difference of germination percentage was shown to be very conspicuous as the NaCl concentration increased. Top dry weight in 166 mM NaCl was decreased by 50% compared with that of control. Inhibition of NaCl appeared to be more in early seedling stage than in germination stage. Top dry weight of cultivars in NaCl stress was not related with germination capacity. Root dry weight in 148mM NaCl decreased by 50% compared with that of control. Root dry weight of cultivars was significantly correlated with top dry weight in NaCl treatment. Top dry weight and root dry weight was higher in diploid type than in tetraploid type at each 8 levels of NaCl concentration. Total chlorophyll content increased with the increase of NaCl concentration and the degree of increase in chlorophyll a was higher than that in chlorophyll b. Chlorophyll a was higher in tetraploid type than in diploid type. But chlorophyll b was higher in diploid type than in tetraploid type. There were significant correlation between chlorophyll a and top dry weight of cultivars over 150mM of NaCl concentration. Free proline content accumulated remarkably in NaCl treatment compared with control and was not difference between diploid type and tetraploid type. Free proline content of cultivars was not related with top dry weight in NaCl treatment.

• Applied Biological Chemistry
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• v.6
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• pp.29-31
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• 1965

The distribution of water soluble free amino acids in various parts of sheep muscles were observed. amino acids of cystine, histidine, aspartic acid, arginine, hydroxy proline, threonine, glutamic acid alanine, proline, methionine, tryptophan, leucine, iso-leucine, glycine, creatine, and two or four different kinds of amino acid content in each tissues, M. longissimus. Overall, the distribution of content of amino acids are seemed to be different for each tissues, while for hydroxyproline the result was negative.

• Applied Biological Chemistry
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• v.9
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• pp.59-64
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• 1968

Takju (Korean native Sake) was mashed with two different materials. One of the material was polished rice and Nuruk(mold wheat), the other one was corn and Nuruk. The amino acids in those fermenting mashes were identified by paper partition chromatography at regular intervals. The results were summarized as follows; a) Following 14 kinds of amino acids were identified in the mash of rice material; lysine, valine, proline, leucine, serine, glycine, aspartic acid, alanine, cystine, tyrosine, histidine, glutamic acid, tryptophan and phenylalanine b) Following 12 kinds of amino acids were identified in the mash of corn material; lysine, valine, proline, leucine, serine, glycine, aspartic acid, alanine, cystine, histidine, argrinine and tryptophan. c) The main amino acids in the Takju mash according to the color density of the each amino acid spot on the paper chromatograms were checked as lysine, valine, leucine, serine, proline and glycine.

• Korean Journal of Agricultural Science
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• v.38 no.1
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• pp.79-86
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• 2011

The contents of free amino acid in cabbage cultivars originated from China and Korea were measured to determine the quality for taste and nutrition. The experimental variables were season (spring-sowing and fall-sowing), leaf position (inside and outside), and the cabbage color (green and red). Eighteen free amino acids were detected at the range of 0-38 mg/g dry weight. The most abundant amino acid was serine(0-128 mg/g). Alanine, aspartate, glutamate, asparagine, histidine, and proline were relatively rich at 2-12 mg/g dry weight. Glycine were significantly increased in spring-sowing cabbages by 2-fold but proline was reversely decreased by 7-fold. The inside parts of cabbages contained significantly more alanine, glutamate, glycine, isoleucine, serine, and valine than the outside ones. The higher contents of isolecuine, leucine, proline, and valine were observed in the red cabbages than the green ones. The significant cross effects of season-color and position-color were also observed, indicating the red and the green cabbages were differently affected by season and tissue position.

• Journal of Life Science
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• v.17 no.2 s.82
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• pp.286-292
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• 2007

Free amino acids and volatile compounds of fresh garlic and its liqueur were investigated to search elution profile of those components as basic data for development of garlic liqueur. The garlic was soaked in 20% alcohol solution and then sampled every week for 5 weeks. The major free amino acids were L-aspartic acid, L-glutamic acid, L-arginine, L-alanine, L-proline, L-asparagine and L-serine. Neutral amino acids such as L-threonine, L-proline, L-valine and L-leucine, and aromatic amino acids such as tyrosine and phenylalanine were eluted over 80% of those content in fresh garlic after 3 weeks of soaking, but acidic, basic and sulfur containing amino acids were below 80% even after 5 weeks. Sulfide compounds such as diallyl trisulfide, diallyl disulfide, methyl allyl disulfide, 2-vinyl-4H-1,3-dithi in, 3-vinyl-3,4-dihydro-1,2-dithiin, 3,5-diethyl-1,24-trithiolane, isobutyl isothiocyanate and diallyl sulfide were identified as major volatile compounds of fresh garlic by using GC/MS. Among volatile compounds of fresh garlic, allyl alcohol, diallyl disulfide, 3,5-diethyl-1,2,4-trithiolane, diallyl trisulfide and 3,4-dimethoxy furan were eluted to liqueur, but those compounds except 3,5-diethyl-1,2,4-trithiolane were lowered in liqueur during soaking. Furfural, 5-methylfurfural, 5-hydroxymethylfurfural, dimethyl pyrazine, furfuryl alcohol, 3-hydroxy-2-bytanone and 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyr-an-4-one were generated newly and their content increased in liqueur during soaking.