• 한국문헌정보학회지
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• 제38권2호
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• pp.291-313
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• 2004

북스타트는 아이들이 유아기부터 책을 가까이하고 성장과정에서 자연스럽게 독서에 흥미를 갖도록 유도하기 위하여 유아들에게 무료로 책을 나누어주는 운동이다. 비영리 단체인 영국의 북트러스트는 1992년에 버빙햄시에서 버빙햄 도서관당국, 남부 버빙햄보건국 그리고 버빙햄대학교와 협력하여 이 운동을 시작하였다. 이 운동은 우리나라뿐만 아니라 세계 여러 나라에 영향을 미쳤다. 현재 우리나라에서는 이 운동이 여러 지방자치단체에 의해서 활발하게 추진될 예정이다. 이에 따라 학계의 많은 연구자, 도서관사서, 각 지방자치단체 공무원 그리고 일반인들이 영국의 북스타트에 대한 자료를 찾고 있다. 본 연구는 이들에게 북스타트 운영에 필요한 유용한 자료를 제공하기 위하여 수행 되었다. 이 연구를 수행하기 위하여 인터뷰, 설문지 그리고 문헌조사가 함께 이루어 졌다. 영국의 북스타트는 아기의 독서 및 책에 대한 흥미 증진뿐만 아니라 부모와 자녀간의 유대관계 게선 그리고 사회적 소외 해결 등 다양한 목적을 가지고 있다.

• 정보교육학회논문지
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• 제11권1호
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• pp.107-116
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• 2007

전자펜을 이용한 문서교정 시스템에서 정확한 교정결과를 보장하기 위해서는 문서 교정자가 드로잉한 교정부호와 문서내용간의 영역 모호성(ambiguity)을 해결하여야 한다. 한편 교정의 대상이 되는 전자문서가 HTML/XML과 같은 경우 교정된 문서구조가 반드시 기 정의된 DTD를 위배하지 않아야 한다. 본 논문에서는 펜 기반의 교정시스템에서 교정부호(마킹)와 대상문서간의 모호성 문제를 최소화하기 위한 기법을 제안한다. 제안 인터페이스에서는 모호성 문제를 최소화하기 위하여 교정부호와 문서간의 컨텍스트(Context)를 반영하였으며 동시에 대상문서의 문서 구조를 유지하기 위한 방법을 제공한다. 그 결과 본 논문에서 제안한 교정 인터페이스는 기존 교정시스템에 비하여 보다 정확한 영역정보를 포함할 수 있으며, 교정부호 입력에 따른 구조문서 변경시에도 원본문서의 DTD에 따르는 문서구조를 유지할 수 있다.

• Journal of Microbiology and Biotechnology
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• 제5권3호
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• pp.117-124
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• 1995

A gene (xynA) encoding the endo-xylanase (E.C.3.2.1.8) from Bacillus stearothermophilus was cloned in E. coli, and its complete nucleotide sequence was determined. The xynA gene consists of a 636 base pairs open reading frame coding for a protein of 212 amino acids with a deduced molecular weight of 23, 283 Da. A putative signal sequence of 27 amino acid residues shows the features comparable with the Bacillus signal sequences; namely, the signal contains a positively charged region close to the N-terminus followed by a long hydrophobic string. The coding sequence is preceded by a possible ribosome binding site with a free energy value of -16.6 kcal/mol and the transcription initiation signals are located further upstream. The translation termination codon (TAA) at the 3 end of the coding sequence is followed by two palindrome sequences, one of which is thought to act as a terminator. The xynA gene has a high GC content, especially in the wobble position of codons (64%). Comparison of the primary protein sequence with those of other xylanases shows a high homology to the xylanases belonging to family G.

• Journal of Microbiology and Biotechnology
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• 제14권5호
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• pp.938-943
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• 2004

A gene responsible for the chlorothalonil-biotransformation was cloned from the chromosomal DNA of Ochrobactrum anthropi SH35B, an isolated bacterium strain from soil. We determined the nucleotide sequences and found an open reading frame for glutathione S-transferase (GST). The drug-hypersensitive Escherichia coli KAM3 cells transformed with a plasmid carrying the GST gene can grow in the presence of chlorothalonil. The GST of O. anthropi SH35B was expressed in E. coli and purified by affinity chromatography. The fungicide chlorothalonil was rapidly transformed by the purified GST in the presence of glutathione. No significant difference in the chlorothalonil-biotransformation effect was observed among the thiol compounds (cysteine, reduced glutathione, and $\beta$-mercaptoethanol). Thus, the result reported here is the first evidence on the chlorothalonil-biotransformation by conjugation with the cellular free thiol groups, especially glutathione, catalyzed by the bacterial GST.

• Journal of Microbiology and Biotechnology
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• 제8권1호
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• pp.28-33
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• 1998

A gene coding for ${\beta}$-xylosidase from thermophilic xylanolytic Bacillus sp. KK-1 was cloned into Escherichia coli using plasmid pBR322. Recombinant plasmid DNAs were isloated from E. coli clones which were capable of hydrolyzing 4-methylumbelliferyl-${\beta}$-D xylopyranoside. Restriction analysis showed the DNAs to share a common insert DNA. Xylo-oligosaccharides, including xylotriose, xylotetraose, xylopentaose, and xylobiose were hydrolyzed to form xylose as an end product by cell-free extracts of the E. coli clones, confirming that the cloned gene from strain KK-1 is ${\beta}$-xylosidase gene. The ${\beta}$-xylosidase gene of strain KK-1 designated as xylB was completely sequenced. The xylB gene consisted of an open reading frame of 1,602 nucleotides encoding a polypeptide of 533 amino acid residues, and a TGA stop codon. The 3' flanking region contained one stem-loop structure which may be involved in transcriptional termination. The deduced amino acid sequence of the KK-1 ${\beta}$-xylosidase was highly homologous to the ${\beta}$-xylosidases of Bacillus subtilis and Bacillus pumilus, but it showed no similarity to a thermostable ${\beta}$-xylosidase from Bacillus stearothermophilus.

• Journal of Microbiology and Biotechnology
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• 제3권3호
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• pp.139-145
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• 1993

The nucleotide sequence of the xylanase gene (xynS) from alkali-tolerant Bacillus sp. YA.14 was determined and analyzed. A 639 base pairs open reading frame for xynS gene was observed and encoded for a protein of 213 amino acids with a molecular weight of 23, 339. S1 nuclease mapping showed that the transcription initiation site of the xynS gene did not exist in the cloned DNA. Ribosome binding site sequence with the free energy of -18.8 Kcal/mol was observed 8 base pairs upstream from the initiation codon, ATG. The proposed signal sequence consisted of 28 amino acids, of which 3 were basic amino acid residues and 21 were hydrophobic amino acid residues. When the amino acid sequences of xylanases were compared, Bacillus sp. YA-14 xylanase showed 48% homology with Bacillus sp. YC-335 xylanase and 96% homology with xylanases from B. subtilis and B. circulans.

• 대한간호학회지
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• 제8권2호
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• pp.15-23
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• 1978

This study was conducted to see the present situation of rest in a military hospital. 120 medical patients but acute af critical cases were interviewed by the investigators during the period of August 20 to September 10, 1978. The questionnaire was consisted of questions regarding the general characteristics of tile respondents, rest methods and nursing activities inducing them to rest. 1, Of the rest methods, tying down on the bed comfortably placed first at 29.1 percent, followed by sleeping at 20,1 percent, reading at 16.8 percent, taking a moderate exercise, being free from their concerns of diseases & surrounding matters at 11.7 percent, respectively, playing the game of Badook or Chess at 6.7 percent , others at 3.9 percent in that order. 2. They mainly utilized the Lying down method for comfort & rest, but there were some differences depending on their educational backgrounds. 3. Some differences were observed in the methods of rest by the religions. 4. The differences between diseases af rest methods except bed rest af sleeping were observed. 5. Of the nursing activities inducing them to rest, counseling stood first at 43.3 percent, followed by teaching at 28.4 percent, relief of pain af discomfort at 20 percent, active attitudes of nurses to help them at 8.3 percent in that order. 6. There was no significant difference between the level of education or religions and nursing activities inducing them to rest. 7. Nursing activities which induced comfort ＆ rest by diseases were mainly counseling & teaching.

• 한국의학물리학회지:의학물리
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• 제9권1호
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• pp.29-35
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• 1998

There has been necessity of an air free ionization chamber using the gold-crystal-aluminium plates, henceforth called the crystal chamber. The crystal chamber formed of parallel plates is very small in size and has more response for absorbed dose of therapeutic radiation beams. The gold plate on the crystal facing the photon and electron beam acts as an intensifier of signals and crystal plate as an ionization medium respectively. Both the copper guard ring and the aluminum collecting electrode are connected to an electrometer. Using high energy photon (6, 15 MV) and electron (9, 12, 15, 18 MeV) beams, the responses of the crystal chamber are evaluated against a PTW Farmer-type chamber at a field size of 10${\times}$10cm$^2$ and 100 cm SSD. The responses of crystal chamber for therapeutic radiation electron and photon beams are greater in magnitude by several order than Farmer. The crystal chamber has good linearity without correction factor C$\_$t,p/ with respect to the signals, a reading reproduction with good accuracy and precision less than 0.5%, and has other useful functions in measuring radiation beams.

• 한국디지털건축인테리어학회논문집
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• 제9권1호
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• pp.49-59
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• 2009

This study examined the programs of public library and the spaces for them in Seoul and Gyunggi area. The examination was performed by making field surveys on 12 sample public libraries and posing user questionnaires on 4 libraries out of the sample. The data from the questionnaire survey were processed and analyzed with SPSS 14.0. The results are as follows: First, according to the arrangement of the furniture, the space usages for the programs in public libraries are divided into four classes, one-way exposure: free of furniture: two-way exposure. The one-way exposure has been the most common though the furniture arrangement varies with the characteristics of a program. Second, language or information technology related programs are the most frequently attended programs in public libraries. Lastly, the space usage for the reading education programs has had the highest level of the user satisfaction measurement while the user satisfaction is lower in those for general culture programs and health care programs.

• Architectural research
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• 제15권2호
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• pp.87-94
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• 2013

Throughout the history of architecture, sometimes the main focus of design was determined by a particular visual representation method, and other times a particular form of visual representation method was required by perception of a particular architectural issue or an architectural form or idea. That is why the visual representation method of architects becomes an important means of reading the flow of idea and thinking behind architecture. This study is an investigation on the relation between architectural thinking and visual representation method expressed through the conceptual drawings by avant-garde architects of the 1970s and 80s, a period of the emergence of postmodernism. Rather than proving the objective reality regarded important by traditional architectural drawing, attempts are made to express the design concept in which the project has its base. Such interpretation and explanation regarding the concept become the main interest of the drawing. It is not that the architecture itself was not expressed in the contents, but it may not be the main subject of expression in the drawing. The value of architectural drawing recovers its value as an art work in itself, as a means of communication, and as an important conceptual tool in the design process. It can be seen that the visual representation method in postmodern architectural drawings is breaking free of the traditional objective depiction of matter and is changing and developing as a design tool of the architect.