• 한국전산구조공학회논문집
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• 제13권3호
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• pp.373-384
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• 2000

단기 동 하중(특히 지진하중)을 받는 비선형 강 프레임 구조물의 안전성을 평가하기 위하여 추계론적 유한요소 개념에 근거한 비선형 시간영역 신뢰성 해석 기법을 제안하였다. 제안된 알고리즘에서는 유한요소 공식화가 응답 표면법, 1차 신뢰성 방법, 그리고 반복 선형보간 기법의 개념들과 결합되어 지는데, 이것이 추계론적 유한요소 개념으로 귀결된다. 실제 지진하중의 시간이력이 구조물의 진동을 위해 사용되므로 사실적인 하중조건의 재현이 가능하다. 가상 응력에 기초한 유한요소 기법이 본 알고리즘의 효율성을 증대하기 위해 사용된다. 본 알고리즘은 지진하중 또는 임의의 단기 동적하중을 받는 유한요소 기법으로 표현되는 어떠한 선형 및 비선형 구조물과 관련된 위험도를 평가할 수 있는 잠재성을 가지고 있다. 수치예제를 통하여 알고리즘을 설명하였으며, 몬테카를로 시뮬레이션 기법을 사용하여 본 알고리즘을 검증하였다.

• Fisheries and Aquatic Sciences
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• 제18권3호
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• pp.273-281
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• 2015

The follistatin (FST) gene encodes a monomeric glycoprotein that plays a role in binding and inhibiting the functions of members of the transforming growth factor (TGF)-${\beta}$ superfamily. Thus, FST facilitates a wide variety of functions, ranging from muscle growth, to inflammation and immunity. In this study, we sought to characterize an FST counterpart, RbFST, which was identified from rock bream Oplegnathus fasciatus. The RbFST cDNA sequence (2,419 bp) contains a 933-bp open reading frame (ORF) that encodes a putative amino acid sequence for RbFST (35 kDa). The putative amino acid sequence contains a Kazal-type serine protease inhibitor domain (51-98 residues) and an EF-hand, calcium-binding domain (191-226 residues). Additionally, this sequence shares a high identity (98.7%) with the Siniperca chuatsi FST sequence, with which it also has the closest evolutionary relationship according to a phylogenetic study. Omnipresent distribution of RbFST transcripts were detected in the gill, liver, spleen, head kidney, kidney, skin, muscle, heart, brain, and intestine of healthy animals, with significantly higher expression levels in the heart, followed by the liver tissue. Under pathogenic stress caused by two bacterial pathogens, Streptococcus iniae and Edwardsiella tarda, RbFST transcription was found to be significantly up-regulated. Altogether, our findings suggest the putative role of RbFST in immune related responses against pathogenic infections, further prefiguring its significance in rock bream physiology.

• 한국약용작물학회지
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• 제13권5호
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• pp.270-275
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• 2005

Superoxide dismutases (SOD) are metalloenzymes that convert $O_2^-\;to\;H_2O_2$. Rehmannia glutinosa is highly tolerant to paraquat-induced oxidative stress. The primary objective of this study was to characterize regulation of SOD gene expression in R. glutinosa in response to oxidative stresses and hormones. A full-length putative SOD clone (RgCu-ZnSOD1) was isolated from the leaf cDNA library of R. glutinosa using an expressed sequence tag clone as a probe. RgCu-ZnSOD1 cDNA is 777 bp in length and contains an open reading frame for a polypeptide consisted of 152 amino acid residues. The deduced amino acid sequence of the clone shows highest sequence similarity to the cytosolic Cu-ZnSODs. The two to three major bands with several minor ones on the Southern blots indicate that RgCu-ZnSOD1 is a member of a small multi-gene family. RgCuZnSOD1 mRNA was constitutively expressed in the leaf, flower and root. The expression of RgCu-ZnSOD1 mRNA was increased about 20% by wounding and paraquat, but decreased over 50% by ethylene and $GA_3$. This result indicates that the RgCu-ZnSOD1 expression is regulated differentially by different stresses and phytohormones at the transcription level. The RgCu-ZnSOD1 sequence and information on its regulation will be useful in investigating the role of SOD in the paraquat tolerance of R. glutinosa.

• 한국화재소방학회논문지
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• 제30권2호
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• pp.123-132
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• 2016

본 연구는 새로운 차체를 이용 구급자동차 내부 공간의 디자인 작업에 따른 차량 내부의 구조해석을 수행한 결과로서, 해외 기준 및 디자인된 구급자동차를 바탕으로 3D 설계 작업을 수행하였다. 또한, 차체 및 구급자동차 내부에 대하여 10G의 충격을 가한 후 이에 따른 구조해석 결과를 살펴보았다. 이때 부품의 자중과 구성품의 무게를 고려하여 유럽의 EN 규정에 따라 해석을 수행하였다. 구조해석 결과, 차량 내부의 프레임과 각종 파이프로 구성되어진 핸드레일은 비교적 큰 응력의 하중은 받지 않았으나 내장 판넬과 캐비넷류는 50MPa 이상의 큰 응력을 받는 것으로 해석되었다. 이에 따른 보강 설계를 실시하는 경우에는 두께 및 형상의 수정이 필요하다는 결론을 얻을 수 있었다. 이와 같은 연구 결과를 바탕으로 국내 구급자동차를 이용하는 구급대원 및 차량내부의 환자에게 보다 안전한 차량을 제작하는 기초자료가 될 수 있을 것이라 기대된다.

• Journal of Ginseng Research
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• 제33권1호
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• pp.59-64
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• 2009

$Ca^{2+}$ and calmodulin (CaM), a key $Ca^{2+}$ sensor in all eukaryotes, have been implicated for defense responses of plants. Eukaryotic CaM contains four structurally and functionally similar $Ca^{2+}$ domains named I, II, III and IV. Each $Ca^{2+}$ binding loop consists of 12 amino acid residues with ligands arranged spatially to satisfy the octahedral symmetry of $Ca^{2+}$ binding. To investigate the altered gene expression and the role of CaM in ginseng plant defense system, cDNA clone containing a CaM gene, designated PgCaM was isolated and sequenced from Panax ginseng. PgCaM, which has open reading frame of 450 nucleotides predicted to encode a precursor protein of 150 amino acid residues. Its sequence shows high homologies with a number of other CaMs, with more similarity to CaM of Daucus carota (AAQ63461). The expression of PgCaM in different P. ginseng organs was analyzed using real time PCR. The results showed that PgCaM expressed at different levels in young leaves, shoots, and roots of 3-week-old P. ginseng. In addition, the expressions of PgCaM under different abiotic stresses were analyzed at different time intervals.

• 한국도로학회논문집
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• 제16권6호
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• pp.95-104
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• 2014

PURPOSES : The purpose of this study is to evaluate the degree of restraint (DOR) of longitudinal steel at continuously reinforced concrete pavement (CRCP) against environmental loadings. METHODS : To measure the longitudinal steel strain, 3-electrical resistance and self-temperature compensation gauges were installed to CRCP test section (thickness = 250mm, steel ratio = 0.7%) and continuously measured 10 min. intervals during 259 days. In order to properly analyze the steel strains first, temperature compensation process has been conducted. Secondly, measured steel strains were divided into 12 phases with different events such as before paving, during concrete hardening, and after first cracking, etc. RESULTS : Thermal strain rate (TSR) concept is defined as the linear strain variations with temperature changes and restraints rate of longitudinal steel against environmental loadings (especially thermal loading) with different cases is defined as degree of restraint(DOR). New concept of DOR could be indirect indicator of crack width behaviors of CRCP. CONCLUSIONS : Before paving, DOR of longitudinal steel is almost same at the coefficient of thermal expansion of steel ($12.44m/m/^{\circ}C$) because of no restraint boundary condition. After concrete pouring, DOR is gradually changed into -1 due to concrete stiffness developing with hydration. After first cracking at crack induced area, values of DOR are around -3~-5. The negative DOR stands for the crack width behavior instead of steel strain behavior. During winter season, DOR reached to -5.77 as the highest, but spring this values gradually reduced as -1.7 as the lowest. Based on this observation, we can presume crack width decreased over time within the time frame of this study. This finding is not consistent with the current theory on crack width variations over time, so further study is necessary to identify the causes of crack width reducing. One of the reasons could be related to concrete stress re-distribution and stress relaxation.

• 한국자동차공학회논문집
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• 제20권5호
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• pp.53-64
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• 2012

Deep and narrow welds can be produced by laser welding at high welding speeds with a narrow heat-affected zone (HAZ) and little distortion of the workpiece. This study aims to evaluate the usefulness of laser welding at automobile component manufacture. Microstructure observation, hardness test, tensile test and fatigue life test are performed by using the fiber laser welded SPFC590 steel sheets which is used widely in the manufacture of automotive seat frame. Three kinds of specimens are only a SPFC590 steel plate, quasi-butt joint plate and lap joint plate by laser welding. The following results that will be helpful to understand the static strength, fatigue crack initiation and growth mechanism were obtained. (1) The tensile strength of quasi butt joint specimens nearly equal to base metal specimens, but lap joint specimens fractured in shear area of weld metal. (2) The fatigue strength of quasi-butt joint specimen was approximately 8 percent lower than that of the base metal specimens. Furthermore, the lap joint specimens were less than 86 percent of the base metal specimens. (3) The lap joint fatigue specimens fractured at shear area in high level stress amplitude, while fractured at normal area in low level stress amplitude. From these results, the applicability of the laser welding to the automobile component is discussed.

• Journal of Plant Biotechnology
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• 제36권4호
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• pp.407-414
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• 2009

A cDNA clone encoding phytocystatin was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (name as Brcpi1; GenBank accession no.: EF079953) had a total length of 881 bp with an open reading frame of 609 bp, and encoded predicted polypeptide of 203 amino acid (aa) residues including a putative N-terminal signal peptide. Other relevant regions found its sequence included the G and PW conserved aa motifs, and the consensus LARFAV sequence for phytocystatins and the reactive site QVVAG. The BrCPI1 protein shared 95, 94, 81, 80 and 78% identity with other CPI proterins isolated from Brassica oleracea (BoCPI-1), Arabidopsis thaliana (AtCY SB), Glycine max (GmCPI), Oryza sativa (OsCYS-2) and Zea may (ZmCPI) at amino acid level, respectively. Southern blot analysis showed that Brcpi1 was a low copy gene. Expression pattern analysis revealed that Brcpi1 was a tissue-specific expressing gene during reproductive growth and strongly expressed at mature seedling stages. Furthermore, overexpression of Brcpi1 in transgenic Arabidopsis was enhanced tolerance to salt and cold stresses. Meanwhile the juvenile seedling of Brcpi1 transgenic plants was not affected by various concentrations ABA in MS medium. Taken together, the results showed that Brcpi1 functioned as a cysteine protease inhibitor and it exhibited a protective agent against diverse types of abiotic stress, which induced this gene in a tissue- and stress-specific manner.

• Journal of Welding and Joining
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• 제17권1호
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• pp.97-103
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• 1999

Ships, structures on the ocean, bridges, and other structures tend to be large by the development of industry. These ultra thick plate were welded with large heat input, which causes welding stresses, deformation and buckling, so it has to be considered the weld design, safety, reliability. The welded residual stresses were produced and redistributed due to the effect of large heat input. The mechanical phenomenon has not been surely identified yet. In spite of the lack of the study on the box column, there are various types of steel frame such as I type, H type, + type and $\bigcirc$ type, used in high story building. In this study, we performed computer simulation with two dimensional heat conduction and plane deformation thermal elasto-plastic finite element computer program as changing the plate thickness to 100mm, 150mm and groove angle to $60^{\circ}C$, $45^{\circ}C$, $30^{\circ}C$ of corner joint in box column. And then, to identify mechanical phenomenon such as the phenomenon of thermal distribution, welding residual stresses and deformation and to decide optimum groove angle and welding condition. The main conclusion can be summarized as follows: 1) Since the groove angle has became cooling down rapidly due to its smaller value, the temperature slope was steeped somewhat. 2) The tensile stress within the welding direction stresses was somewhat decreased at the weld metal and HAZ, increasing of the groove angle. 3) The local stress concentration of the groove angle $60^{\circ}C$ was appeared smaller than groove angle $30^{\circ}$.

• 한국수산과학회지
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• 제48권3호
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• pp.354-361
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• 2015

Mitochondrial heat shock protein 75 (mtHSP75) is a member of the HSP90 family and plays essential roles in refolding proteins of the mitochondrial matrix. Mitochondria provide energy in the form of ATP and generate reactive oxygen species (ROS). Heat shock proteins (HSPs) are activated in response to stress, and protect cells. In this study, we characterized the mtHSP75 of the big-belly seahorse Hippocampus abdominalis. The protein (BsmtHSP75) is encoded by an open reading frame (ORF) of 2,157 nucleotides, has 719 amino acids (aa), and is of molecular mass 82 kDa. BsmtHSP75 has two functional domains, a histidine kinase-like ATPase (HATPase_c) domain (123-276 aa) and an HSP90 family domain (302-718 aa). BsmtHSP75 was expressed in all tested tissues of healthy seahorses. The ovary contained the highest transcription level, followed (in order) by the blood, brain, and muscle. Pouch tissue showed the lowest expression level. The expression of BsmtHSP75 was significantly (P<0.05) up-regulated on viral or bacterial challenge, suggesting that BsmtHSP75 plays a role in the immune defense against bacterial and viral pathogens.