• 한국발생생물학회지:발생과생식
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• 제1권2호
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• pp.157-164
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• 1997

To study the regulation of porcine follicular cell apostosis by gonadotropin, steroid, and nitric oxide, we analyzed DNA fragmentation, the hallmark of apoptosis, and nitrite production of porcine granulosa cells. Dissected indiidual follicles from ovary were separated in size (small, 2-3 mm; medium, 5-6 mm; large, 7-8 mm) and isolated granulosa cells were classified morpholocally as atretic or nonatretic. Nitrite concentration was measured by mixing follicular fluids with an equal volume of Griess reagent. Follicular nitric oxide (NO) concentration of healthy follicles was higher than that of atretic follicles. Apoptotic DNA fragmentation was suppressed in non-apoptotic granulosa cells. Follicular apoptosis was induced by androgen but prevented by gonadotropin in vitro. Apoptosis was confined to the granulosa cells. But it was not clear whether apoptosis of granulosa cells were isolated, incubated with or without gonadotropin, androgen and sodium nitroprusside (SNP), respectively at $37^{\circ}C$ for 24 hrs. Cultured granulosa cells were used to extract genomic DNA and culture media was asssayed for nitrite concentration. Nitrite production of culture media was increased, while apoptotic DNA fragmentation was suppressed in PMSG, hCG, testosterone+SNP and SNP treated groups. Nitrite concentration in culture media was decreased, but apoptotic DNA fragmentation was induced in testosterone treated group. These data suggest that NO production and apoptosis may be involved of granulosa cell apoptosis induced by testosterone.

• Asian-Australasian Journal of Animal Sciences
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• 제15권7호
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• pp.1045-1050
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• 2002

The aims of this study were to detect spontaneously occurring apoptosis in cultured porcine ovarian cells, to examine the role of growth hormone (GH), tyrosine kinase (TK), protein kinase G (PKG) and cyclin-dependent kinase (CDK) in the control of this process, and to determine whether the effect of GH on apoptosis is mediated by TK-, PKG- and cdc2-dependent intracellular mechanisms. We studied the action of pGH (10 ng/ml), blockers of TK (genistein, lavendustin, both 100 ng/ml), PKG (Rp-Br-PET-cGMPS, 50 nM; KT5823, 100 ng/ml) and CDK (olomoucine, $1{\mu}g/ml$), as well as combinations of GH with these blockers, on the onset of apoptosis in cultured granulosa cells isolated from antral (3-6 mm) porcine follicles. The functional characteristics of an early apoptotic event, DNA fragmentation, were determined using terminal deoxynucleotidyltransferase (TdT)-mediated dUTP nick end labelling (TUNEL), whilst morphological signs of advanced apoptosis such as pyknosis, chromatin marginalization, shrinkage and fragmentation of nucleus, were detected using routine light microscopy. After culture, some ovarian granulosa cells exhibited DNA fragmentation, which in some cases was associated with morphological apoptosis-related changes (pyknosis, shrinkage and fragmentation of the nucleus). GH significantly reduced the proportion of TUNEL-positive cells. Neither TK nor CDK blockers when given alone, significantly affected the percentage of TUNEL-positive cells although both PKG blockers significantly increased this index. Furthermore, TK and PKG blockers given together with GH, prevented or reversed the inhibitory effect of GH on apoptosis, whilst the CDK blocker olomoucine promoted it. These observations demonstrate apoptosis in porcine ovaries and suggest the involvement of GH, TK, PKG and CDK in the control of this process. They also suggest that the effect of GH on ovarian apoptosis is mediated or regulated by multiple signalling pathways including TK-, PKG- and CDK-dependent intracellular mechanisms.

• 대한한의학회지
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• 제22권4호
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• pp.37-44
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• 2001

Objectives : Cellular death by apoptosis is an active process, depending on gene transcription and protein synthesis. It was reported that nitric oxide can induce apoptosis in several cancer cell-lines. We have previously observed that proliferation of Ll210 cells was inhibited by the administration of Gleditsiae Spina water extract (GE). In this present study, the mechanism of inhibitory action on the proliferation of L l210 cells was examined. Methods : The cell proliferation was determined by MTT assay and DNA fragmentation was determined by a flow cytometry. Results : The administration of GE decreased proliferation of L1210 cells and enhanced DNA fragmentation in vivo system. DNA fragmentation of L1210 cells was enhanced by co-culture of peritoneal macrophages obtained from GE-administered mice in vitro and it was partly inhibited by L-NMMA, nitric oxide synthetase inhibitor. In addition, GE increased nitric oxide production from peritoneal macrophages of L1210-transplanted mice. Conclusions : These results suggest that the inhibitory action of GE on proliferation of transplanted-L1210 cells is partly caused by an induction of apoptosis via production of nitric oxide in macrophages.

• East Asian Economic Review
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• 제24권4호
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• pp.389-416
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• 2020

This paper provides empirical evidence on the reorganization of GVCs in East Asia, highlighting that structural trends explain a decrease in the fragmentation of production after 2011 but that it is not the result of rising trade costs along the value chain. Using harmonized inter-country input-output tables, the paper first analyzes the global import intensity of production to document changes in the structure of GVCs. It then calculates theory-consistent bilateral trade costs for intermediate and final products using an approach derived from the gravity literature and introduces a new index of cumulative trade costs along the value chain. These data are used to discuss whether the decrease in global imports is the consequence of shifts in demand, efficiency-enhancing strategies of firms or rising trade costs. Between 2011 and 2016, cumulative trade costs have decreased in East Asian GVCs. However, as COVID-19 is likely to intensify trade and investment uncertainties, trade costs could increase in the future. Policies aimed at reducing uncertainties and preserving the gains from trade and investment liberalization will be key in this new environment.

• 화약ㆍ발파
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• 제36권4호
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• pp.9-15
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• 2018

발파에 의한 암석의 파쇄도는 적재, 운반과 2차 파쇄로 이어지는 후속공정에 직접적인 영향을 미치므로 파쇄도의 조절은 발파 효율성과 생산비용을 평가하는데 필수적이다. 본 연구에서는 인공절리의 방향에 따른 암석의 파쇄도에 대해 분석하였다. 갱내 암반에 수직 및 수평인공절리를 생성한 후 발파실험을 수행하였다. 생성된 암석파쇄물의 입도분포는 2차원 영상해석 프로그램인 split-desktop으로 평가하였다. 평가결과 수평인공절리가 수직인공절리에 비해 전체적인 암석파쇄물의 크기가 작게 평가되었고, 다양한 크기로 암석파쇄물이 분포하였다. 인공절리의 방향에 따라 대괴발생을 억제하고 암석파쇄물을 일정한 크기 이하로 조절 할 수 있을 것으로 판단된다.

• 화약ㆍ발파
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• 제32권3호
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• pp.1-9
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• 2014

벤치발파에서 발파에 의한 암석의 파쇄입도는 적재, 운반과 파쇄로 이어지는 후속공정에 직접적으로 영향을 미치고 있다. 본 연구에서는 전자뇌관을 사용하여 정확한 지연시차를 구현하였으며, 각 발파공에 대해 저항선 m당 1, 2, 3, 4, 5, 7, 10ms의 지연시차를 적용하여 실규모 시험을 통해 암석 파쇄입도를 측정하였다. 그 결과, 석회석 광산에서 저항선을 기준으로 약 6ms/m의 지연시차가 최소의 파쇄입도를 가지는 것으로 측정되었다. 또한, 파쇄입도 분포 분석을 통해 지연시차가 암석의 파쇄입도에 변화를 줄 수 있는 요소로 정의될 수 있으며, 지연시차를 통해 파쇄입도를 조절 가능하다고 사료된다.

• Environmental Analysis Health and Toxicology
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• 제19권3호
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• pp.251-259
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• 2004

Xenoestrogens are chemicals with diverse structure that mimic estrogen. Bisphenol A, a monomer of polycarbonate and epoxy resins, has been detected in canned food and human saliva. Bisphenol A stimulate cell proliferation and induce expression of estrogen -response genes in vitro. The purpose of the this study was to evaluate cell proliferation of bisphenol A in the presence of a rat liver 59 mix contaning cytochrome P450 enzymes and Cu (II). The fragmentation of intact DNA, a parameter of apoptotic cell death, was evaluated quantitatively by diphenylamine reaction method. Bisphenol A induced apoptotic cell death in a dose-dependent manner The effect of radical scavenger on the apoptotic cell death induced bisphenol A was investigated. The DNA fragmentation induced by bisphenol A was significantly inhibited by addition of radical scavenger to the culture medium. This indicated that elevated oxidative stress caused by imbalance between the production and removal of free radicals occurred in cells. Taken together, these results suggest that free radical reacts with Cu (II) leading oxidative stress.

• Geomechanics and Engineering
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• 제14권2호
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• pp.151-159
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• 2018

Annually, the global production of construction aggregates reaches over 40 billion tons, making aggregates the largest mining sector by volume and value. Currently, the aggregate industry is shifting from sand to hard rock as a result of legislation limiting the extraction of natural sands and gravels. A major implication of this change in the aggregate industry is the need for understanding rock fragmentation and energy absorption to produce more cost-effective aggregates. In this paper, we focused on incorporating dynamic rock and soil mechanics to understand the effects of loading rate and water saturation on the rock fragmentation and energy absorption of three different sandstones (Red, Berea and Buff) with different pore sizes. Rock core samples were prepared in accordance to the ASTM standards for compressive strength testing. Saturated and dry samples were subsequently prepared and fragmented via fast and dynamic compressive strength tests. The particle size distributions of the resulting fragments were subsequently analyzed using mechanical gradation tests. Our results indicate that the rock fragment size generally decreased with increasing loading rate and water content. In addition, the fragment sizes in the larger pore size sample (Buff sandstone) were relatively smaller those in the smaller pore size sample (Red sandstone). Notably, energy absorption decreased with increased loading rate, water content and rock pore size. These results support the conclusion that rock fragment size is positively correlated with the energy absorption of rocks. In addition, the rock fragment size increases as the energy absorption increases. Thus, our data provide insightful information for improving cost-effective aggregate production methods.

• 터널과지하공간
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• 제14권5호
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• pp.353-362
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• 2004

벤치발파에서 암석 파쇄도 예측은 생산계획을 수립하는 데 있어서 가장 중요한 요소 중의 하나이다. Kua-Ram 모델은 지금까지 제안된 암석 파쇄도 예측 모델 중 가장 우수한 것으로 평가받고 있으나, 이 모델의 평가항목을 구성하고 있는 절리조건, 암석강도, 밀도, 사용폭약의 성능과 저항선, 공간격 등의 요소들은 그 값을 선정하는 과정에서 주관적인 요소가 개입되거나 정의가 모호한 요소를 포함하고 있다. 이 연구에서는 Kuz-Ram 모델을 구성하는 여러 평가항목의 값을 선정하는 과정에서 주관적이거나 모호한 요소를 제거하는 방법에 대해 검토하였으며, 예측값을 현장조사 결과와 비교하여 적용성을 검토하였다. Kuz-Ram 모델은 비교적 정확한 예측결과를 보였으나, 현지암반 조건을 충분히 반영하지 못하여 개선이 필요한 것으로 판단되었다.

• 지반과기술
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• 제3권3호
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• pp.15-22
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• 2006

The optimum blasting pattern to excavate a quarry efficiently and economically can be determined based on the minimum production cost, which is generally estimated according to rock fragmentation. Therefore, it is a critical problem to predict fragment size distribution of blasted rocks over an entire quarry. By comparing various prediction models, it can be ascertained that the result obtained from Kuz-Ram model relatively coincides with that of field measurements. Kuz-Ram model uses the concept of rock factor to signify conditions of rock mass such as block size, rock jointing, strength and others. For the evaluation of total production cost, it is imperative to estimate 3-D spatial distribution of rock factor for the entire quarry. In this study, a sequential indicator simulation technique is adopted for estimation of spatial distribution of rock factor due to its higher reproducibility of spatial variability and distribution models than Kriging methods. Further, this can reduce the uncertainty of predictor using distribution information of sample data. The entire quarry is classified into three types of rock mass and optimum blasting pattern is proposed for each type based on 3-D spatial distribution of rock factor. In addition, plane maps of rock factor distribution for each ground level are provided to estimate production costs for each process and to make a plan for an optimum blasting pattern.