• 한국통신학회논문지
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• 제21권7호
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• pp.1746-1755
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• 1996

In this paper, we propose the protocol stact and the server functions of IWU which interconnects distributed LAN's users by using of B-ISDN. The protocol stact of interconnecting IWU under consideration users TCP/IP in upper layer. TCP/IP is popular communication protocol in interconnecting distributed LANs. The interconnecting IWU has server functions for transfering datagrams to B-ISDN such as address translation, fragment and reassembly, CL server, signalling and traffic control. We analyze the performance ofinterconnecting IWU with M/M/1/K queueing model and obtain the throughput and buffer size of interconnecting IWU.

• 미생물학회지
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• 제26권1호
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• pp.6-12
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• 1988

Cellobiase ($\beta$-glucosidase) is an enzyme of the cellulase system in cellulolytic microor-ganisms. The chromosomal DNA fragment which include cellobiase gene of Cellulomonas biazotea was cloned in Eschericia coli via plasmid pBR 322 vector. Restriction enzyme Sal I was used to obtain adequate size of fragments from C. biazotea. chromosomal DNA. The transformant of E. coli HB101 with recombinant plasmid pBG101 showed cellobiase activity, which is not ordinary in E. coli HB101. The enzyme activity of the transformant was as of 20% lower than that of C. biazotea.

• 한국미생물·생명공학회지
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• 제18권6호
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• pp.640-646
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• 1990

E.coli의 cytidine deaminase(cytidine/2'-deoxy-cytidine aminohydrolas` EC 3.5.4.5)를 코딩하는 cdd 유전자를 E.coli cdd- pyr- 결손 변이주를 cloning host로 하여 southern blotting과 colony hybridization을 통하여 클로닝하였다. cdd 유전자가 단편인, cdd 유전자의 transcription initiation 부위의 23개 nucleotide를 합성한 후 probe로 사용하여 Southern hybridization에 의해 회수된 cdd 유전자를 함유한 단편을 얻었으며, 이를 pBR322에 삽입한 후 형질전환하여 colony hybridization한 결과 cdd+ cell을 얻었다. 삽입된 DNA 단편의 size는 27kb이었으며 이를 결실 및 subcloning을 연속 수행한 결과 2.1kb의 SalI/ DraI fragment(pTK605)에 cdd 유전자가 location 되어 있음을 알게 되었다. Mini cell 실험결과 합성된 cytidine deaminase의 활성이 pBR322에서 증폭시킴으로서 37배 정도 배가되었으며, pBR322에 비해 pUC vector계에서 다시 활성이 7배 정도 증가됨을 알 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제27권5호
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• pp.965-974
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• 2017

The single-chain variable fragment (scFv) against lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a promising molecule for its potential use in the diagnosis and immunotherapy of atherosclerosis. Producing this scFv in several milligram amounts could be the starting point for further engineering and application of the scFv. In this study, the abundant expression of the anti-LOX-1 scFv was attempted using Escherichia coli (E. coli) and Brevibacillus choshinensis (B. choshinensis). The scFv had limited soluble yield in E. coli, but it was efficiently secreted by B. choshinensis. The optimized fermentation was determined using the Plackett-Burman screening design and response surface methodology, under which the yield reached up to 1.5 g/l in a 5-L fermentor. Moreover, the properties of the scFvs obtained from the two expression systems were different. The antigen affinity, transition temperature, and particle diameter size were 1.01E-07 M, $55.2{\pm}0.3^{\circ}C$, and 9.388 nm for the scFv expressed by B. choshinensis, and 4.53E-07 M, $52.5{\pm}0.3^{\circ}C$, and 13.54 nm for the scFv expressed by E. coli. This study established an efficient scale-up production methodology for the anti-LOX-1 scFv, which will boost its use in LOX-1-based therapy.

• 대한족부족관절학회지
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• 제9권1호
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• pp.94-98
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• 2005

Purpose: To compare the radiographic evaluations between two radiographic views (AP and lateral views) and four radiographic views (AP, lateral and both oblique views) in ankle fractures. Materials and Methods: From January 2000 to June 2002, 60 cases of ankle fractures were treated with open reduction and internal fixation and its followed up periods were at least 2 years. All cases were classified into three groups according to the method of preoperative radiographic evaluation. Two radiographic views (AP and lateral views) were taken in group A and four radiographic views (AP, lateral and both oblique views) were taken in group B. 12 Cases were evaluated with three dimensional computed tomography (3D CT). Four radiographic views and 3D CT were taken in group C. All cases were classified according to the Danis-Weber and Lauge-Hansen classification. Displacement of fracture fragment of medial, lateral, posterior malleolus and size of fracture fragment of posterior malleous were measured using picture archiving communication system (PACS). Results: Although kappa value between two or four radiographic views were good or excellent in Danis-Weber classification and Lauge-Hansen classification of ankle fractures, the displacements of medial and lateral malleoli were statistically different. Four radiographic views evalulated the degree of displacement of medial and lateral fragments more accurately compared to two radiographic views. Conclusion: Four radiographic views will be more useful than two radiographic views to decide the method of treatment and operation considering the displacement of fracture fragment.

• 한국통신학회논문지
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• 제33권4A호
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• pp.450-457
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• 2008

에너지 효율적 전송기술은 무선 PAN(WPAN: Wireless Personal Area Network) 장치들과 같이 에너지 소모에 있어서 제약을 많이 받는 무선장치의 동작 시간을 최대화하기 위해서 필수적으로 요구되는 핵심 기술이다. 더욱이, WPAN이 TDMA 동작 방식과 연동되어 수행될 때, 할당된 각 time 슬롯에서 실제 물리적 전송의 QoS 요구 사항에 대한 보장은 설계시 만족시켜야 하는 또 다른 중요 설계 고려사항이다. 따라서, 본 논문에서는 TDMA 기반의 비경합 매체 접근 프로토콜과 함께 동작하는 WPAN을 위한 에너지-효율적이며 동시에 QoS를 고려한 전송 방법을 제안한다. 제안한 알고리즘을 통해서 전송파워, 물리적 데이터 전송율(변조 수준/방식), 에너지 소모 최소화, 그리고 동시에 할당된 time 구간에서 요구되는 QoS 만족시키는 fragment 크기 등에 대한 최적 조합을 결정할 수 있다. 제안한 알고리즘과 기존의 알고리즘과의 차이점은 전송파워, 데이터 전송율 및 fragment 크기에 대한 모든 파라미터의 조합을 찾는 것이다. 시뮬레이션 결과는 제안한 알고리즘을 통하여 처리량과 에너지 소모 측면에서 성능이 향상되었음을 보인다.

• Journal of Microbiology
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• 제39권1호
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• pp.37-41
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• 2001

The fission yeast Schizosaccharomyces pombe contains two distinct superoxide dismutase (SOD) activities, one in the cytosol encoded by the $sod2^{+}$ gene and the other in mitochondria. The $sod2^{+}$ gene encoding putative mitochondrial manganese superoxide dismutase (MnSOD) was isolated from the S. pombe genomic library using a PCR fragment as the probe. The nucleotide sequence of the $sod2^{+}$ gene and its flanking region (4051 bp HindIII fragment) was determined. An intron of 123 nt in size was predicted and confirmed by sequencing the cDNA following reverse transcription PCR. The predicted Sod2p consists of 218 amino acid residues with a molecular mass of 24,346 Da. The deduced amino acid sequence showed a high degree of homology with other MnSODs, especially in the metal binding residues at the active site and their relative positions. The transcriptional start site was mapped by primer extension at 231 at upstream from the ATG codon. A putative TATA box(TATAAAA) was located 58 nt upstream from the transcriptional start site and putative polyadenylation sites were located at 1000, 1062, and 1074 nt downstream from the ATG start codon.

• 통합자연과학논문집
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• 제10권2호
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• pp.78-84
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• 2017

CXC chemokine receptor 2 (CXCR2) is a prominent chemokine receptor on neutrophils. CXCR2 antagonist may reduce the neutrophil chemotaxis and alter the inflammatory response because the neutrophilic inflammation in the lung diseases is found to be largely regulated through CXCR2 receptor. Hence, in the present study, Hologram based Quantitative Structure Activity Relationship Study was performed on a series of CXCR2 antagonist named pyrimidine-5-carbonitrile-6-alkyl derivatives. The best HQSAR model was obtained using atoms, bonds, and chirality as fragment distinction parameter using hologram length 151 and 6 components with fragment size of minimum 4 and maximum 7. Significant cross-validated correlation coefficient ($q^2=0.774$) and non cross-validated correlation coefficients ($r^2=0.977$) were obtained. The model was then used to evaluate the six external test compounds and its $r^2_{pred}$ was found to be 0.614. Contribution map show that presence of cyclopropyl ring and its bulkier substituent's makes big contributions for improving the biological activities of the compounds. We hope that our HQSAR model and analysis will be helpful for future design of novel and structurally related CXCR2 antagonists.

• 통합자연과학논문집
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• 제8권1호
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• pp.1-12
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• 2015

CRTh2 receptor is an important mediator of the inflammatory effects and act as beneficial target for the treatment of asthma, COPD, allergic rhinitis and atopic dermatitis. In the present work, Hologram QSAR studies were conducted on a series of 50 training set CRTh2 antagonists (2-(2-(benzylthio)-1H-benzo[d]imidazol-1-yl acetic acids). The best HQSAR model was obtained using atoms, bonds, connections and donor/acceptor as fragment distinction parameter using hologram length 257 and 6 components with fragment size of minimum 7 and maximum 10. Significant cross-validated correlation coefficient ($q^2=0.786$) and non cross-validated correlation coefficients ($r^2=0.954$) were obtained. The model was then used to evaluate the 15 external test compounds which are not included in the training set and the predicted values were in good agreement with the experimental results ($r^2_{pred}=0.739$). Contribution map show that presence of C ring and its substituents makes big contributions for activities. The HQSAR model and analysis from the contribution map could be useful for further design of novel structurally related CRTh2 antagonists.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2005년도 제24회 춘계학술대회논문집
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• pp.47-52
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• 2005

이 논문은 열폭발 실험에서의 열적, 화학적, 기계적 행동의 결과에 대한 3차원 모델 결과를 나타낸다. 폭발이 관찰되기 전까지 제한된 고 폭발물은 시간당 $1^{\circ}C$의 비율로 가열된다. 임의의 Lagrangian-Euler 코드를 사용하여 모델링된 가열, 점화 그리고, deflagration 단계는 구조적에서 동적인 hydro 시간단계까지 변하는 넓은 범위의 시간 영역에서 다루어 질수 있다. Johnson-Cook Failure Model (JCFM)에 실험적 고장분포를 더하여 폭발기기의 균열방향과 fragment의 크기를 예측할 수 있는 모델을 개발한다.