• 대한본초학회지
• /
• 제39권3호
• /
• pp.49-56
• /
• 2024

Objective : Obesity, which has recently been rapidly increasing in the obese population, is caused by an imbalance in energy intake and consumption. The reason why we need to manage obesity well is that the prevalence of complications such as diabetes, atherosclerosis, insulin resistance, and cardiovascular disease increases. In this study, the effect of FO (Fragaria orientalis) water extract on fat metabolism in 3T3-L1 cells was observed to develop a new anti-obesity material based on Mongolian medical books. Methods : The effect of FO extract on adipogenesis in 3T3-L1 cells was observed using DPPH scavenging, pancreatic lipase inhibitory activity, MTT analysis and Oil-red-O staining method. And the expression of proteins related to lipid metabolism was analyzed by Western blot. Results : The FO group significantly increased the DPPH radical scavenging activity at 5 mg/ml compared to the positive control BHA at 0.1 mg/ml. In oil red O staining at a safe concentration without cytotoxicity, lipid accumulation was significantly inhibited by less than 80% compared to the control group at all concentrations. Moreover, treatment of FO significantly increased the expression of proteins related to lipid metabolism, such as p-AMPK and p-ACC, in 3T3-L1 cells, and the expression of CPT-1 tended to increase in a dose-dependent manner. However, the expression of PPAR-γ was significantly decreased in a dose-dependent manner. Conclusion : These results suggest that FO water extract has a potential anti-obesity effect and are expected to be utilized in the development of materials for obesity prevention and treatment.

• 대한본초학회지
• /
• 제38권3호
• /
• pp.1-10
• /
• 2023

Objective : Obesity in modern society has a significant impact on pathological, psychological and social problems. Therefore, many studies on obesity treatment using herbal extracts with fewer side effects have been conducted. This study was designed to investigate the effect of inhibiting fat accumulation in vitro in order to Mongolian medicinal plants find anti-obesity candidate among. Methods : We measured pancreatic lipase inhibitory activity in Mongolian medicinal plants extract. Cytotoxicity of these extracts was monitored in 3T3-L1 cells by MTT assay. In addition, the anti-obesity effects was confirmed at concentrations of 0.2, 0.1 and 0.05 mg/㎖ through Oil red O staining. Results : Among Mongolian medicinal plants, Rheum undulatum roots in September (RURS), Paeonia anomala L. (PAL), and Fragaria orientalis (FO) showed the highest pancreatic lipase inhibitory activity. As a result of the MTT assay, more than 80% was judged to be non-toxic, and the concentration was determined, and as a result of evaluating the lipid accumulation inhibitory effect, 6 types were selected as candidates. Conclusion : Based on these results, the top 7 species expected to be used as anti-obesity functional materials were selected. However, additional efficacy verification and mechanism of action need to be established in the future. So, it is expected that the medicinal plants verified through this will be used as functional materials for the prevention and treatment of obesity.

• 식물병연구
• /
• 제13권3호
• /
• pp.183-190
• /
• 2007

우리나라에서 1994년부터 2004년까지 73 식물체의 흰가루병균으로부터 중복기생균 Ampelomyces quisqualis를 308 균주 분리하였다. 그중에서 A. quisqualis의 새로운 균기주(mycohost)와 식물기주(plant host)는 38 식물체에서 흰가루병균 8 속(Erysiphe, Sphaerotheca, Microsphaera, Phyllactinia, Podosphaera, Uncinula, Uncinuliella, Oidium) 13종이 발견되었다. A. quisqualis의 새로 발견된 기주는 어수리의 흰가루병균(Erysiphe heraclei), 꽃향유의 흰가루병균(E. hommae), 콩의 흰가루병균(E. glycines), 싸리의 흰가루병균(E. lespedezae), 모시물퉁이의 흰가루병균(E. pileae), 녹두의 흰가루병균(E. pisi), 토대황과 소리쟁이의 흰가루병균(E. polygoni), 쑥의 흰가루병균(Golovinomyce artemisiae), 삼잎국화의 흰가루병균(G. cichoracearum), 꼭두서니의 흰가루병균(G. rubiae), 쥐오줌풀, 가는잎왕고들빼기, 두메담배풀, 흰까실쑥부쟁이, 수세미오이, 씀바귀, 풀협죽도, 도깨비바늘과, 담쟁이덩굴의 흰가루병균(Oidium sp.), 갈참나무의 흰가루병균(Microsphaera alphitoides), 댕댕이덩굴의 흰가루병균(M. pseudolonicerae), 쥐똥나무의 흰가루병균(Podosphaera sp.), 딸기의 흰가루병균(Sphaerotheca aphanisi), 물봉선의 흰가루병균(S. balsaminae), 국수호박, 쥬키니호박, 단호박, 관상용호박, 곰취, 가지, 박, 참외, 깨풀, 코스모스와 참취의 흰가루병균(S. fusca), 찔레꽃의 흰가루병균(Uncinuliella simulans)과 배롱나무의 흰가루병균(Uncinula australiana)이다.