• Title/Summary/Keyword: Fraction of free base

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FORMULATION AND STABILITY TEST OF ANTIAGING CREAM CONTAINING METHANOL FRACTION OIL OF PANGIUM EDULE.REINW. AS A RADICAL SCAVENGER AGENT

  • Djajadisastra, J.;Anwar, E.;Milani, E.
    • Proceedings of the SCSK Conference
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    • 2003.09b
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    • pp.303-306
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    • 2003
  • Indonesia is a tropical country having a temperature range of 25-35$^{\circ}C$ which can affect the skin and causes damages like aging. This aging process is due, at least, to free radical reactions. For this reason, many attempts had been done to find out creams containing natural antioxidant compound which have a potential of free radical scavenger. Kluwek, a fermented form of foot ball fruit or picung (Pangium edule.Reinw), had been proved to contain antioxidant compound in its methanol fraction oil to which antiaging cream was formulated. Stability evaluation was conducted for cream with Kluwek oil compared to base cream, including organoleptic (colour and odour), pH, viscosity, particle size, centrifugation test and flow characteristics either in room temperature (27$^{\circ}C$) or stress condition (4$^{\circ}C$ and 5$0^{\circ}C$) for 8 weeks continuously, and six times cycling test at 4$^{\circ}C$ and 5$0^{\circ}C$ every 24 hours. The results showed that cream with Kluwek oil and base cream were stable at temperature 27 and 4$^{\circ}C$, cycling test and centrifugation test, but not stable at 5$0^{\circ}C$. Free radical evaluation was done by Electron Spin Resonance and the result showed that cream with Kluwek oil had less free radicals compared to base cream.

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Structural damping for soil-structure interaction studies

  • Lutes, Loren D.;Sarkani, Shahram
    • Structural Engineering and Mechanics
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    • v.3 no.2
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    • pp.107-120
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    • 1995
  • A soil-structure interaction formulation is used here which is based on consideration of the dynamics of the structure with a free, rather than a fixed, base. This approach is shown to give a quite simple procedure for coupling the dynamic characteristics of the structure to those of the foundation and soil in order to obtain a matrix formulation for the complete system. In fixed-base studies it is common to presume that each natural mode of the structure has a given fraction of critical damping, and since the interaction formulation uses a free-base model, it seems natural for this situation to assign the equal modal damping values to free-base modes. It is shown, though, that this gives a structural model which is significantly different than the one having equal modal damping in the fixed-base modes. In particular, it is found that the damping matrix resulting in equal modal damping values for free-based modes will give a very significantly smaller damping value for the fundamental distortional mode of the fixed-base structure. Ignoring this fact could lead one to attribute dynamic effects to interaction which are actually due to the choice of damping.

Calculation of Free Nicotine by Determination of pH and Nicotine in Tobacco (담배 중 pH와 Nicotine 함량에 의한 Free nicotine 측정)

  • Lee Jeong-Min;Jang Gi-Chul;Hwang Keon-Joong;Kim Yong-Ha;Rhee Moon-Soo
    • Journal of the Korean Society of Tobacco Science
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    • v.27 no.2
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    • pp.219-225
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    • 2005
  • Characterizing nicotine delivery from tobacco products is important in the understanding of their addictive potential. Most previous studies report total nicotine and have not differentiated between nicotine in its protonated or free-base form. The amount of free nicotine calculated by determining pH and nicotine contents. The pH and nicotine contents in smokeless tobacco product, tobacco products and tobacco leaves were analyzed by Health Canada-Official Method T-310 and CORESTA Recommended Method $N^{\circ}62$. The content of free nicotine was calculated according to the Henderson-Hasselbalch equation and the value of $\alpha_{fb}$(the fraction of nicotine that is in the free base form) by using a pKa value of 8.02 for nicotine. The percentage of free nicotine then was calculated by dividing the free nicotine content by total nicotine content. The pH value and percentage of free nicotine ranged from 5.01 to 5.45 and $0.10\%\;to\;0.27\%$ in cut tobacco and 5.10 to 7.10 and $0.12\%\;to\;10.73\%$ in tobacco leaves, respectively.

Cytotoxic Effects and Components of Lipid Fractions from Soybean Products on Cancer Cell Lines (대두식품 지질추출물의 세포독성 및 지질성분분석)

  • 송성광;김광혁;김희숙
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.6
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    • pp.1266-1271
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    • 2001
  • The cytotoxic effects of lipid extracts from soybean products were studied using K562 human leukemia cell, Yac1 mouse leukemia cell and S 180 mouse sarcoma cell. Total lipids from soybean powder, soybean curd residue and doenjang were extracted with chloroform/methanol (2 : 1) and water saturated butanol, consecutively, and fractionated into acetone supernatants (AS fraction) and acetone precipitates (AP fraction) by adding excess acetone. AS fraction of doenjang lipids showed the strongest cytotoxic effects on K562, Yac1 and S180 cancer cells, whereas each lipid fraction of soybean curd residue also showed relatively weak cytotoxic effects on cancer cells but soybean powder did not. AS and AP fractions of doenjang contained more free fatty acids than those of soybean curd residue and soybean. And when lipid fractions were digested with 0.4 N KOH/methanol, doenjang lipid fractions showed to contain some alkali-stable substances which showed positive reaction with ninhydrin solution on silica TLC separation.

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Formation of Thicker hard Alloy Layer on Aluminum Alloy by PTA Overlaying with Metal Powders (플라스마 아크 紛體肉盛法에 의한 Al 合金의 硬化厚膜 合金化層의 形成)

  • ;;中田一博;松田福久
    • Journal of Welding and Joining
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    • v.11 no.2
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    • pp.74-85
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    • 1993
  • Effect of Si metal powders addition with the plasma transferred arc(PTA) overlaying process on characteristics of the alloyed layer in aluminum alloy(A5083) has been investigated. The overlaying conditions were 175-250A in plasma arc current, 500mm/min in travel speed, the 5-20g/min in powder feeding rate. Main results obtained are summarized as follows. 1)Sufficient size of molten pool on surface of base metal was required for forming an alloyed layer; in a fixed travel, the formation of alloyed layer with clear and beautiful surface depend upon the plasma arc current and powder feeding rate; the greater plasma arc current and the smaller powder feeding rate were, the better bead was formed. Optimum alloyed conditions by which an excellent alloyed bead obtained was 225A in plasma arc current. PTA process made it possible to form an alloyed layer with up to 67wt% Si. 2)Microstructure in the alloyed layer was in accord with prediction from the Al-Si phase diagram 3)The hardness of the alloyed layer increased in proportion to Si content. 4)As volume fraction of primary Si increased, the specific wearness of the alloyed layer was significantly improved. However, no further improvement was found when the volume fraction was greater than about 30%. 5)Utilizing the PTA process, a crack free alloyed layer with maximum hardness of about Hv 310 could be obtained.

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An analytical study on free vibration of magneto electro micro sandwich beam with FG porous core on Vlasov foundation

  • Kazem Alambeigi;Mehdi Mohammadimehr;Mostafa Bamdad
    • Advances in nano research
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    • v.15 no.5
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    • pp.423-439
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    • 2023
  • The aim of this paper is to investigate the free vibration behavior of the micro sandwich beam composing of five layers such as functionally graded (FG) porous core, nanocomposite reinforced by carbon nanotubes (CNTs) and piezomagnetic/piezoelectric layers subjected to magneto electrical potential resting on silica aerogel foundation. The effect of foundation has been taken into account using Vlasov model in addition to rigid base assumption. For this purpose, an iterative technique is applied. The material properties of the FG porous core and FG nanocomposite layers are considered to vary throughout the thickness direction of the beams. Based on the Timoshenko beam theory and Hamilton's principle, the governing equations of motion for the micro sandwich beam are obtained. The Navier's type solution is utilized to obtain analytical solutions to simply supported micro sandwich beam. Results are verified with corresponding literatures. In the following, a study is carried out to find the effects of the porosity coefficient, porous distribution, volume fraction of CNT, the thickness of silica aerogel foundation, temperature and moisture, geometric parameters, electric and magnetic potentials on the vibration of the micro sandwich beam. The results are helpful for the design and applications of micro magneto electro mechanical systems.

Evaluation of Antioxidant Activities of Peptides Isolated from Korean Fermented Soybean Paste, Chungkukjang

  • Kim, Sun-Lim;Chi, Hee-Youn;Kim, Jung-Tae;Hur, On-Sook;Kim, Deog-Su;Suh, Sae-Jung;Kim, Hyun-Bok;Cheong, Ill-Min
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.4
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    • pp.349-360
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    • 2011
  • The objectives of present study were to characterize the peptides which were isolated from Korean fermented soybean paste, chungkukjang, and to determine their antioxidant activities. Four fractions were collected from the methanol extract of chungkukjang by using a recycling preparative HPLC. Among fractions, Fr-2 was identified to be highly potent free radical scavenging activity in the assay of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and nitroblue tetrazolium(NBT)-reduction inhibition. Base on antioxidant effects, fraction Fr-2 was employed for the refraction with a prep-column and separated into five fractions of which two fractions were identified to have higher antioxidant activity. To confirm the amino acid constituents of antioxidant fractions Fr-2-2 and Fr-2-3 were analyzed, and eight kinds of amino acids such as aspartic acid, threonine, serine, glutamic acid, glycine, lysine, histidine, and arginine were identified as the constituent amino acids. Antioxidant activities of the separated peptides were further assessed cell viability with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl terazolium bromide (MTT), and fluorescence-activated cell sorting (FACS) analysis of H4IIE cells treated with hydrogen peroxide (H2O2). Chungkukjang peptides have shown their ability to protect H4IIE rat hepatoma cells against H2O2- induced oxidative stress by concentration and time-dependent manner. Therefore, These results indicated that fermented soybean paste chungkukjang will be promoted the antioxidant and radical scavenging activities, and beneficial for health. The antioxidant peptide fractions Fr-2-2 and Fr-2-3 were denominated as P-NICS-1 and P-NICS-2, respectively. However, further studies were required to clarify their amino acid sequences and molecular properties, and physiological significances.

Performance of carbon fiber added to anodes of conductive cement-graphite pastes used in electrochemical chloride extraction in concretes

  • Pellegrini-Cervantes, M.J.;Barrios-Durstewitz, C.P.;Nunez-Jaquez, R.E.;Baldenebro-Lopez, F.J.;Corral-Higuera, R.;Arredondo-Rea, S.P.;Rodriguez-Rodriguez, M.;Llanes-Cardenas, O.;Beltran-Chacon, R.
    • Carbon letters
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    • v.26
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    • pp.18-24
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    • 2018
  • Pollution of chloride ion-reinforced concrete can trigger active corrosion processes that reduce the useful life of structures. Multifunctional materials used as a counter-electrode by electrochemical techniques have been used to rehabilitate contaminated concrete. Cement-based pastes added to carbonaceous material, fibers or dust, have been used as an anode in the non-destructive Electrochemical Chloride Extraction (ECE) technique. We studied the performance of the addition of Carbon Fiber (CF) in a cement-graphite powder base paste used as an anode in ECE of concretes contaminated with chlorides from the preparation of the mixture. The experimental parameters were: 2.3% of free chlorides, 21 days of ECE application, a Carbon Fiber Volume Fraction (CFVF) of 0.1, 0.3, 0.6, 0.9%, a lithium borate alkaline electrolyte, a current density of $4.0A/m^2$ and a cement/graphite ratio of 1.0 for the paste. The efficiency of the ECE in the traditional technique using metal mesh as an anode was 77.6% and for CFVF of 0.9% it was 90.4%, with a tendency to increase to higher percentages of the CFVF in the conductive cement-graphite paste, keeping the pH stable and achieving a homogeneous ECE in the mass of the concrete contaminated with chlorides.

A Study on the Mutagenicity of Thermally Oxidized Safflower Oil (가열산화 홍화유의 돌연변이원성에 관한 연구)

  • 안명수;이진영
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.1
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    • pp.120-127
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    • 2000
  • Deep-fat frying is a common cooking practice. There has been considerable concern regarding the mutagenic and carcinogenic potential of thermally oxidized oils. Studies on deep-fried foods so far have revealed not much on the mutagenicity of the oils in the foods. Therefore, in the present study, it was attempted to investigate the mutagenicity ofthe thermally oxidized safflower oil. Oil was heated in a home-fryer at a temperature of 180$\pm$3$^{\circ}C$ for 48 hours. Oil samples were taken at 0, 8, 16, 24, 32, 40 and 48 hours of heating, respectively. Each sample was used to study the changes in peroxide value (POV), acid value (AV), iodine value (IV), conjugated dienoic acid (CDA) content, %, and fatty acid composition. Another series of samples were fractionated into non-polar and polar fractions by column chromatography. The mutagenicity of the samples taken from the thermally oxidized oils, as well as the non-polar and polar fractions of the thermally oxidized oils, was investigated with the Ames test. The Ames test was carried out with and without metabolic activation. Bacterial tester strains used in the present study were the histidine auxotrophic strains of Salmonella typhimurium TA100, TA1535 and TA102 were used for the detection of base pair mutations, and TA98 and TA1537 for frame shift mutations. Each series of samples was dissolved in tetraphydrofuran (inhibitor-free) and tested at doses ranging from 0.05 to 5 mg/plate. None of the oil samples taken during the 48 hour oxidation period showed any mugagenic activity. This was the case, even after the activaton with 59 mix. Also, none of the polar and non-polar fractions showed any mutagenic activity on all the strains tested.

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