• Journal of Animal Reproduction and Biotechnology
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• v.36 no.4
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• pp.307-313
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• 2021

Traf4 (Tumor necrosis factor Receptor Associated Factor 4) is a member of the tumor necrosis factor receptor (TNFR) - associated factors (TRAFs) family. TRAF4 is overexpressed in tumor cells such as breast cancer and associated with cytoskeleton and membrane fraction. Interestingly, TRAF4 was localized with tight junctions (TJs) proteins including OCLN and TJP1 in mammary epithelial cells. However, the expression patterns and biological function of Traf4 were not examined in preimplantation mouse embryos although Traf4-deficient mouse showed embryonic lethality or various dramatic malformation. In this study, we examined the temporal and spatial expression patterns of mouse Traf4 during preimplantation development by qRT-PCR and immunostaining, and its biological function by using siRNA injection. We found upregulation of Traf4 from the 8-cell stage onwards and apical region of cell - cell contact sites at morula and blastocyst embryos. Moreover, Traf4 knockdown led to defective TJs without alteration of genes associated with TJ assembly but elevated p21 expression at the KD morula. Taken together, Traf4 is required for TJs assembly and cell proliferation during morula to blastocyst transition.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.4
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• pp.2385-2390
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• 2015

In the beginning run, urban railway had been required as transportation. But now days urban railway have stayed in the platform for long time, the platform is faced the problem that is improvement of environment as one of the living space. Thus, sliding automatic door on the basis of screen door have used in huge distribution market, hospital, restaurant and public office because it is comfortable that customer's convenience and entrance are controled. So screen door not only requires customer's convenience and safe, clean area and energy conservation but demands optimal design technology development of screen door system that is confirmed by element parts of design and confidence. In this paper, For secure confidence of screen door, after as modeling roller and frame's system, confirming the result for qualification of driving stiffness. And then it suggests that it is possible to increase performance and declines fraction defective of element's part.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.2
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• pp.605-610
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• 2011

Soft ground improvement is essential to enhance strength of ground for construction in reclaimed land or shore. There are many method of soft ground improvement, and vertical drain method was widely used in many countries including korea. As vertical drain method is to plant many Prefabricated Vertical Drains in soft ground, it promotes consolidation and enhances strength. The PBD(Plastci Board Drain) that is excellent economy and workability was widely used in many countries as Prefabricated Vertical Drains. Construction quality of PBD is affected installation depth, pressure, perpendicularity. This paper describes the system developed that can automatically measure installation depth, pressure and perpendicularity for PBD. This system can reduce fraction defective of construction by auto faulty alarm and keeps the safety of operator by auto control system.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.8
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• pp.4736-4741
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• 2014

This study examined PCB component cleaning on a PCB component surface, which has defects of precipitation type washing (existing rinse method), sealant and foreign material formed in the adhesive process that could not be removed easily. The spin jig was developed for PCB component cleaning, in which the PCB component settled down, to solve the conventional problem of the removal of foreign material with the centrifugal force by high speed rotation. The results are as follows. With decreasing fraction defect in PCB component washing, the development and substrate damage decreased by more than 80% according to the abstergent in the rotary type using the centrifugal force in the existing precipitation type. When the base plate showed a large difference with the time to include the process after washing the design using the existing method, easy attachment and separation of the PCB component could be possible. The washing time was enhanced 90% compared to the existing time. The reliability of the security and washing collaboration of the design and stability of the cleaning process could be secured so that there was no phenomenon of secession, the PCB component fixed for a cleansing rotation jig could maintain a fixed force by the centrifugal force. The stability and reliability of the washing process and the defective rate could be improved to less than 1%.