• Title/Summary/Keyword: Four-component

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Genome-wide Screening to Identify Responsive Regulators Involved in the Virulence of Xanthomonas oryzae pv. oryzae

  • Han, Sang-Wook;Lee, Mi-Ae;Yoo, Youngchul;Cho, Man-Ho;Lee, Sang-Won
    • The Plant Pathology Journal
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    • v.35 no.1
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    • pp.84-89
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    • 2019
  • Two-component systems (TCSs) are critical to the pathogenesis of Xanthomonas oryzae pv. oryzae (Xoo). We mutated 55 of 62 genes annotated as responsive regulators (RRs) of TCSs in the genome of Xoo strain PXO99A and identified 9 genes involved in Xoo virulence. Four (rpfG, hrpG, stoS, and detR) of the 9 genes were previously reported as key regulators of Xoo virulence and the other 5 have not been characterized. Lesion lengths on rice leaves inoculated with the mutants were shorter than those of the wild type and were significantly restored with gene complementation. The population density of the 5 mutants in planta was smaller than that of PXO99A at 14 days after inoculation, but the growth curves of the mutants in rich medium were similar to those of the wild type. These newly reported RR genes will facilitate studies on the function of TCSs and of the integrated regulation of TCSs for Xoo pathogenesis.

Experiment for Duct Development to Cool Components in Vehicle Engine Room (차량 엔진룸내 부품 냉각용 덕트 개발을 위한 실험)

  • Lee, Suk Young
    • Journal of Energy Engineering
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    • v.27 no.4
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    • pp.98-102
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    • 2018
  • In order to improve the durability of components in the vehicle engine room, an experiment to improve the air cooling effect of components by installing a cooling duct using intake air aiming at four components, such as generator, battery, ECU and power steel oil, Respectively, experimental results show that the overall component temperature has been reduced, and the reduced temperature difference is in the order of generator, ECU, power steel oil and battery. In order to improve the temperature difference due to these components, it is necessary to optimize the design through the flow analysis in the duct in the future.

A Study on City Brand Evaluation Method Using Text Mining : Focused on News Media (텍스트 마이닝 기법을 활용한 도시 브랜드 평가방법론 연구 : 뉴스미디어를 중심으로)

  • Yoon, Seungsik;Shin, Minchul;Kang, Juyoung
    • Journal of Information Technology Services
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    • v.18 no.1
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    • pp.153-171
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    • 2019
  • Competition among cities has become fierce with decentralization and globalization, and each city tries to establish a brand image of the city to build its competitiveness and implement its policies based on it. At this time, surveys, expert interviews, etc. are commonly used to establish city brands. These methods are difficult to establish as sampling methods an empirical component, the biggest component of a city brand. In this paper, therefore, based on the precedent research's urban brand measurement and components, the words representing each city image property were extracted and relocated to five indicators to form the evaluation index. The constructed indicators have been validated through the review of three experts. Through the index, we analyzed the brands of four cities, Ulsan, Incheon, Yeosu, and Gyeongju, and identified the factors by using Topic Modeling and Word Cloud. This methodology is expected to reduce costs and monitor timely in identifying and analyzing urban brand images in the future.

Studies on CFST column to steel beam joints using endplates and long bolts under central column removal

  • Gao, Shan;Yang, Bo;Guo, Lanhui;Xu, Man;Fu, Feng
    • Steel and Composite Structures
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    • v.42 no.2
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    • pp.161-172
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    • 2022
  • In this paper, four specimens of CFST column joints with endplates and long bolts are tested in the scenario of progressive collapse. Flush endplate and extended endplate are both adopted in this study. The experimental results show that increasing the thickness of the endplate could improve the behavior of the joint, but delay the mobilization of catenary action. The thickness of the endplate should not be relatively thick in comparison to the diameter of the bolts, otherwise catenary action would not be mobilized or work effectively. Effective bending deformation of the endplate could help the formation and development of catenary action in the joints. The performance of flexural action in the joint would affect the formation of catenary action in the joint. Extra middle-row bolts set at the endplates and structural components set below the bottom beam flange should be used to enhance the robustness of joints. A special weld access hole between beam and endplate should be adopted to mitigate the chain damage potential of welds. It is suggested that the structural components of joints should be independent of each other to enhance the robustness of joints. Based on the component method, a formula calculating the stiffness coefficient of preloaded long bolts was proposed whose results matched well with the experimental results.

Time uncertainty analysis method for level 2 human reliability analysis of severe accident management strategies

  • Suh, Young A;Kim, Jaewhan;Park, Soo Yong
    • Nuclear Engineering and Technology
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    • v.53 no.2
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    • pp.484-497
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    • 2021
  • This paper proposes an extended time uncertainty analysis approach in Level 2 human reliability analysis (HRA) considering severe accident management (SAM) strategies. The method is a time-based model that classifies two time distribution functions-time required and time available-to calculate human failure probabilities from delayed action when implementing SAM strategies. The time required function can be obtained by the combination of four time factors: 1) time for diagnosis and decision by the technical support center (TSC) for a given strategy, 2) time for strategy implementation mainly by the local emergency response organization (ERO), 3) time to verify the effectiveness of the strategy and 4) time for portable equipment transport and installation. This function can vary depending on the given scenario and includes a summation of lognormal distributions and a choice regarding shifting the distribution. The time available function can be obtained via thermal-hydraulic code simulation (MAAP 5.03). The proposed approach was applied to assess SAM strategies that use portable equipment and safety depressurization system valves in a total loss of component cooling water event that could cause reactor vessel failure. The results from the proposed method are more realistic (i.e., not conservative) than other existing methods in evaluating SAM strategies involving the use of portable equipment.

Characteristics of Hybrid Protective Materials with CNT Sheet According to Binder Type

  • Jihyun Kwon;Euisang Yoo
    • Elastomers and Composites
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    • v.57 no.4
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    • pp.197-204
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    • 2022
  • Recently, the demand has increased for protective clothing materials capable of shielding the wearer from bullets, fragment bullets, knives, and swords. It is therefore necessary to develop light and soft protective clothing materials with excellent wearability and mobility. To this end, research is being conducted on hybrid design methods for various highly functional materials, such as carbon nanotube (CNT) sheets, which are well known for their low weight and excellent strength. In this study, a hybrid protective material using CNT sheets was developed and its performance was evaluated. The material design incorporated a bonding method that used a binder for interlayer combination between the CNT sheets. Four types of binders were selected according to their characteristics and impregnated within CNT sheets, followed by further combination with aramid fabric to produce the hybrid protective material. After applying the binder, the tensile strength increased significantly, especially with the phenoxy binder, which has rigid characteristics. However, as the molecular weight of the phenoxy binder increased, the adhesive force and strength decreased. On the other hand, when a 25% lightweight-design and high-molecular-weight phenoxy binder were applied, the backface signature (BFS) decreased by 6.2 mm. When the CNT sheet was placed in the middle of the aramid fabric, the BFS was the lowest. In a stab resistance test, the penetration depth was the largest when the CNT sheet was in the middle layer. As the binder was applied, the stab resistance improvement against the P1 blade was most effective.

Effects of Carbide and Matrix Structures on Abrasion Wear Resistance of Multi-Component White Cast Iron (다합금계 백주철의 탄화물 및 기지조직이 내마모성에 미치는 영향)

  • Ryu, Seong-Geun
    • Korean Journal of Materials Research
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    • v.7 no.4
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    • pp.310-316
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    • 1997
  • The effects of carbide and matrix structures on the abrasion wear resistance of multi-component white cast irons with 3.0 mass%C have been studied in this paper. Four different heats were poured in order to obtain the specimens with different combinations of the carbide structures: a basic iron(3.0 mass%C-5.0 mass%Cr-5.0 mass%V-5.0 mass% Mo-12.5mass%W)for M$_{6}$C and M$_{7}$C$_{3}$ carbides, and a Cr free iron(3.0 mass%C-5.0 mass%V-2.5mass%Mo-12.5 mass%W) for MC and M6C carbides. A conventional high Cr free free iron(3.0 mass%C-5.0 mass%V-2.5 mass%Mo-12.5 mass%W) for MC and M6C carbides. A conventional high Cr white cast iron was also poured to compare its wear resistance with those of the multi-component white cast irons. In the as-cast condition, the range of abrasive wear rate(Rw=mg/min) was from 4.15 to 5.98 . The lowest Rw, which means the highest wear resistance, was obtained in the basic iron with nodular MC, lamellar M$_{2}$C and cellular M$_{7}$C$_{3}$ carbides. On the other hand, the Rw of the high Cr white cast iron ranked between the basic iron and the Mo and W free iron. In each alloy, the Rw of air hardened or tempered specimen was lower than that of the as-cast one because of the change of matrix structures by the heat treatments. The Rw of the hear treated speci-mens increased in the order Mo and W free iron, basic iron, Cr free iron, high Cr iron, and V free iron.n.n.n.

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Probabilistic seismic assessment of RC box-girder bridges retrofitted with FRP and steel jacketing

  • Naseri, Ali;Roshan, Alireza Mirzagoltabar;Pahlavan, Hossein;Amiri, Gholamreza Ghodrati
    • Coupled systems mechanics
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    • v.9 no.4
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    • pp.359-379
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    • 2020
  • Due to susceptibility of bridges in the past earthquakes, vulnerability assessment and strengthening of bridges has gained a particular significance. The objective of the present study is to employ an analytical method for the development of fragility curves, as well as to investigate the effect of strengthening on the RC box-girder bridges. Since fragility curves are used for pre-and post-earthquake planning, this paper has attempted to adopt the most reliable modeling assumptions in order to increase the reliability. Furthermore, to acknowledge the interaction of soil, abutment and pile, the effect of different strengthening methods, such as using steel jacketing and FRP layers, the effect of increase in the bridge pier diameter, and the effect of vertical component of earthquake on the vulnerability of bridges in this study, a three-span RC box-girder bridge was modeled in 9 different cases. Nonlinear dynamic analyses were carried out on the studied bridges subjected to 100 ground motion records via OpenSEES platform. Therefore, the fragility curves were plotted and compared in the four damage states. The results revealed that once the interaction of soil and abutment and the vertical component of the earthquake are accounted for in the calculations, the median fragility is reduced, implying that the bridge becomes more vulnerable. It was also confirmed that steel jackets and FRP layers are suitable methods for pier strengthening which reduces the vulnerability of the bridge.

A Cross-national Study of Social Exclusion - with Special References to Korea and European Countries - (한국의 사회적 배제 성격에 대한 연구 - 유럽연합 회원국과의 비교를 중심으로 -)

  • Moon, Jin-Young
    • Korean Journal of Social Welfare
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    • v.62 no.2
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    • pp.87-107
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    • 2010
  • This paper purports to compare the differences and similarities of social exclusions in Korea with those in the European countries, notably Germany, France, Sweden, Finland, Ireland and U.K. For this purpose, chapter two examines political and social origins of social exclusion as an alternative concept to poverty or unemployment. Chapter three discusses social exclusion paradigms of Silver(1994) in reference to welfare regime theories of Esping-Andersen(1990). Subsequently chapter four, using two artificial social exclusion indicators by principal component analysis, defines the basic nature of social exclusion of Korea in comparison with those of the six European countries. Chapter five duly concludes that social exclusions in Korea are very similar to those of liberal countries (Ireland and U.K.) in that income indicators (poverty and inequality) of Korea are much worse, whereas unemployment rate is relatively low.

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Verification of the Reliability and Validity of the Short Form 36 Scale in Indonesian Middle-aged and Older Adults

  • Arovah, Novita Intan;Heesch, Kristiann C.
    • Journal of Preventive Medicine and Public Health
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    • v.53 no.3
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    • pp.180-188
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    • 2020
  • Objectives: The Short Form 36 (SF-36) questionnaire is increasingly being used to measure health-related quality of life (HRQoL) in Indonesia. However, evidence that it is valid for use in Indonesian adults is lacking. This study assessed the validity and reliability of the SF-36 in Indonesian middle-aged and older adults. Methods: Adults aged 46-81 years (n=206) in Yogyakarta, Indonesia completed the SF-36, another measure of HRQoL (the EuroQoL visual analogue scale [EQ-VAS]), and measures assessing their demographic characteristics. Fifty-four percent (n=121) completed the SF-36 measure again 1 week later. Confirmatory factor analysis was conducted to confirm the factor structure of the SF-36. Internal consistency reliability was estimated using Cronbach's alpha, and test-retest reliability was assessed using intraclass correlations. Convergent and discriminant validity were assessed by computing correlations among SF-36 subscales, between subscales and the 2 component scores, and between component scores and EQ-VAS scores. Results: Most scaling assumptions were met. The hypothetical factor structure fit the data poorly (root mean square error of approximation [RMSEA]=0.108) and modification was required for a good fit (RMSEA=0.060). Scores on all subscales demonstrated acceptable internal consistency (α>0.70) and test-retest reliability (r>0.70). Divergent validity was supported by weak to moderate interscale correlations (r=0.19 to 0.64). As expected, the 2 summary scores were moderately to strongly correlated with the EQ-VAS (r>0.60). Conclusions: The findings adequately support the use of SF-36 in Indonesian middle-aged and older adults, although the optimal algorithm for computing component scores in Indonesia warrants further investigation.