The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Genome-wide Screening to Identify Responsive Regulators Involved in the Virulence of Xanthomonas oryzae pv. oryzae

  • Han, Sang-Wook (Department of Integrative Plant Science, Chung-Ang University) ;
  • Lee, Mi-Ae (Graduate School of Biotechnology, Kyung Hee University) ;
  • Yoo, Youngchul (Graduate School of Biotechnology, Kyung Hee University) ;
  • Cho, Man-Ho (Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Sang-Won (Graduate School of Biotechnology, Kyung Hee University)
  • Received : 2018.09.19
  • Accepted : 2018.10.16
  • Published : 2019.02.01
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Abstract

Two-component systems (TCSs) are critical to the pathogenesis of Xanthomonas oryzae pv. oryzae (Xoo). We mutated 55 of 62 genes annotated as responsive regulators (RRs) of TCSs in the genome of Xoo strain PXO99A and identified 9 genes involved in Xoo virulence. Four (rpfG, hrpG, stoS, and detR) of the 9 genes were previously reported as key regulators of Xoo virulence and the other 5 have not been characterized. Lesion lengths on rice leaves inoculated with the mutants were shorter than those of the wild type and were significantly restored with gene complementation. The population density of the 5 mutants in planta was smaller than that of PXO99A at 14 days after inoculation, but the growth curves of the mutants in rich medium were similar to those of the wild type. These newly reported RR genes will facilitate studies on the function of TCSs and of the integrated regulation of TCSs for Xoo pathogenesis.

Keywords

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Fig. 1. Virulence phenotypes of PXO99A (WT) and RR mutant strains. (A) Lesion lengths caused by PXO99A (WT, black), RR mutant (white), and the complementary (gray) strains on Dongjin rice leaves at 14 days after infection (DAI). Rice leaves were infected with 107 cfu/ml of bacterial suspension by the scissor clipping method. Bars are mean ± SD (n = 20). * or ** indicate that the lesion length of each mutant was significantly different from that of PXO99A by Duncan’s test (P < 0.05). (B) Bacterial population at 0 (white) and 14 (gray) DAI. Immediately after infection (0 DAI) and at 14 DAI, 1-cm2 and 25-cm2 samples from the infected site of three leaves were used to extract the bacteria population at 0 and 14 dpi, respectively. Bars are mean ± SD (n = 3). * and ** indicate that the population of mutants was significantly smaller than that of PXO99A by Duncan’s test (P < 0.05). C) All experiments were repeated three times with high consistency, and the results from one experiment are shown.

Table 1. Sixty-two candidate RR genes in the PXO99A genome

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Table 1. Continued

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