Kim, Mi Joung;Chung, Jee-Young;Kim, Jung Hee;Kwak, Ho-Kyung
Nutrition Research and Practice
/
v.7
no.6
/
pp.430-438
/
2013
Increased oxidative stress in obese diabetes may have causal effects on diabetic complications, including dyslipidemia. Lipopolysccharides (LPS) along with an atherogenic diet have been found to increase oxidative stress and insulin resistance. Cranberry has been recognized as having beneficial effects on diseases related to oxidative stress. Therefore, we employed obese diabetic animals treated with an atherogenic diet and LPS, with the aim of examining the effects of cranberry powder (CP) on diabetic related metabolic conditions, including lipid profiles, serum insulin and glucose, and biomarkers of oxidative stress. Forty C57BL/KsJ-db/db mice were divided into the following five groups: normal diet + saline, atherogenic diet + saline, atherogenic diet + LPS, atherogenic diet + 5% CP + LPS, and atherogenic diet + 10% CP + LPS. Consumption of an atherogenic diet resulted in elevation of serum total cholesterol and atherogenic index (AI) and reduction of high density lipoprotein (HDL)-cholesterol. However, with 10% CP, the increase in mean HDL-cholesterol level was close to that of the group with a normal diet, whereas AI was maintained at a higher level than that of the group with a normal diet. LPS induced elevated serum insulin level was lowered by greater than 60% with CP (P < 0.05), and mean serum glucose level was reduced by approximately 19% with 5% CP (P > 0.05). Mean activity of liver cytosolic glutathione peroxidase was significantly increased by LPS injection, however it was reduced back to the value without LPS when the diet was fortified with 10% CP (P < 0.05). In groups with CP, a reduction in mean levels of serum protein carbonyl tended to occur in a dose dependent manner. Particularly with 10% CP, a reduction of approximately 89% was observed (P > 0.05). Overall results suggest that fortification of the atherogenic diet with CP may have potential health benefits for obese diabetes with high oxidative stress, by modulation of physical conditions, including some biomarkers of oxidative stress.
A method for the multiresidual simultaneous analysis of 11 thiocarbamates was studied using HPLC. Thiocarbamate in Chinese cabbage was analyzed in the order of extraction, partition, and cleanup in their optimum condition. Acetone was chosen as an extracting solvent. As a partitioning solvent, the mixture of 50% methylene chloride and petroleum ether containing extremely small water content showed good recoveries of thiocarbamate from the water layer. Partition efficiency was affected by pH of the water layer; it remained almost constant under the acidic and neutral condition while decreasing under the basic condition. The comparison done in cleanup step showed that the column chromatographic method is superior to the treatment of coagulating reagent. As an absorbent, the mixture of charcoal, magnesia, and celite with the ratio of 1 : 2 : 4 gave better recoveries and also effectively removed chlorophyll. Over the total procedure, the average recoveries for thiocarbamates in Chinese cabbage were 91% at about 2 ppm fortification level within the relative standard deviation of 8%, and the minimum detection limit (MDL) was 2.2${\sim}$9.3 ng.
Background: Cyanazine is used as a pre-emergent herbicide once during the growing season to control weeds of many upland crops worldwide. This study aimed to establish a method to determined cyanazine residue levels in major medicinal crops by using high performance liquid chromatography-UV detection/mass spectometry (HPLC-UVD/MS). Methods and Results: Cyanazine residue was extracted with acetone from the raw products of four representative medicinal plants - Scutellaria baicalensis, Paeonia lactiflora, Platycodon grandiflorum and Angelica gigas. The extract was diluted with a large volume of saline water and directly partitioned into dichloromethane to remove polar co-extractives in the aqueous phase. It was then purifined using optimized Florisil column chromatography. HPLC analysis conducted using an octadecylsilyl column allowed the successful separation of cyanazine from co-extractives of the samples, and the amount was sensitively quantified by ultraviolet absorption at 225 nm with no interference. The accuracy and precision of the proposed method were validated by conducting recovery experiments on each medicinal crop sample fortified with cyanazine at two concentration levels per crop in triplicate. Conclusions: The mean recoveries ranged from 91.2% to 105.3% for the four representative medicinal crops. The coefficients of variation were less than 10%, irrespective of the sample types and fortification levels. The limit of quantification of cyanazine was 0.02 mg/kg as verified by the recovery experiment. A confirmatory method was performed by liquid chromatography/MS using selected-ion monitoring technique to clearly identify the suspected residue.
Journal of the Korean Society of Food Science and Nutrition
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v.20
no.4
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pp.363-368
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1991
To fortificate protein to soybean curd, 0, 20, 40, 60 and 80% (v/v) of egg white (EW) were added to soybean milk for the soybean curd preparation, respectively. Moisture, $Ca^{2+}$, crude lipid and ash content of the curd were decreased as EW increased whereas protein content, weight and volume, $Mg^{2+},\;K^{+},\;and\;Na^{+}$ incresed, and hardness also increased. In addition, coagulating temperature and hardness were lowered as EW increased. Color and taste panel score were not significantly different (p<0.01), however, texture and flavor score were lowered over 60% (v/v) EW addition. By adding EW (20, 40, 60 and 80%), sulfur containing amino acids were enriched 0.63, 1.20, 1.76 and 2.36 times, respectively compared to the control(0%).
Periodically, the Japanese Castle was created in the domestic of Japan and then 2 Invasions into Chosun was started. The Japanese Castle in the domestic of Japan was repaired several times by the building boom of castle before & after 2 invasions and so the initially-built type of castles was changed. Accordingly, there are much difficulties to understand the original shape of Japanese Castle. Through the Japanese Castle within Korea called as the fossil of Japanese Castle, I would like to examine & consider its building period and characteristics. The terminology called as [Two Side Stone's Wall] is that of castle which is not acknowledged in the Japanese Academic Circles. However, it means the two-fold wall of Japanese Castle which was widely applied to the fortification way in the Age of Japan Edo. The terminology of [Sori] says the stonework curve in the corner of Japanese Castle which is indicated best in the Japanese Castle. It calls the curve as like the fan frame. [Curb Stone's Wall] says the type of castle wall constructed with over 1 face in wall body of Japanese Castle. (1) About classifying the construction period of Japanese Castle, the curb stone's wall and the castle having no two side stone's wall must consider the building period as that of Japanese Invasion of Korea in 1592. If there was [Sori], the two-side stone's wall was used and the place which supported the documentary data, in particular, the place having the record of contraction is considered to be confirmed as the castle constructed in the period of Japanese Invasion of Korea in 1597. (2) The two-side-type stone's wall shown in the Modern Japanese Stone Castle is difficultly considered to be generated from the Japanese Castle at the period of Japanese Invasion of Korea in 1592 and in 1597. (3) The beautiful [Sori] shown in the Modern Japanese Stone Castle was started from the Japanese Castle of Korea at the period of Japanese Invasion of Korea in 1597. It is difficultly considered which its indication was firstly generated by the Chaesung-Folded Segment Structure.
So far, the main threat to South Korea was North Korea. That is why South Korea established a strategy based on the threat of North Korea and most of the budget on defense was used to deter North Korea. Even though the neighboring countries(China, Japan, and Russia) are growing as a real threat with abilities and intentions based on their powerful naval forces, South Korea has not yet been able to establish a strategy that regards neighboring countries as a threat. But the decades-old structural mechanism of the Korean security environment is undergoing a radical change on April 27, 2018, through the South-North summit and the Panmunjom Declaration. Under the changing security environment, South Korea was placed in a complicated dilemma that had to deal with threats of two axes(China), three axes(China, Japan), and four axes(Japan, Russia). If the one axis threat(North Korea) is dominated by land threats, the second, third and fourth axis threats are threats from the sea. This paper analyzed the maritime strategy of Korea within the framework of maritime-geopolitics, in other words recognition and expansion of the sphere of maritime. I have designed that the maritime defense space that we can deny from threats is divided into three lines of defense: 1 line (radius 3,000km), 2 lines (2,000km), and 3 lines (1,000km). The three defense zones of the three lines were defined as an active defense(1 line), defensive offense(2 line), active offense(3 line). The three defense zones of the three lines were defined as the sphere of core maritime, As a power to deny the sphere of core maritime, it was analyzed as a maneuvering unit, a nuclear-powered submarine, the establishment of missile strategy, and the fortification of islands station. The marine strategy of South Korea with these concepts and means was defined as 'Offensive Maritime Denial Strategy'.
Feasibility of microbial culture media using by-product of salt fermented kanary was investigated. Gram negative strain, Escherichia coli, and Gram positive strain, Bacillus subtilis, and bioluminescent Photobacterium Phosphoreum were incubated with kanary by-Product media (KB media). Compared with LB media, KB media had enough carbon source, but lacked nitrogen source and growth factor. When 0.5% of peptone as a nitrogen source and 0.3% of yeast extract as nitrogen and growth factor source were fortified in KB media, the cell population rate was similar to LB media. Also, when 0.5% of yeast extract was fortified to KB media, it showed the same result as in LB media. The price of KB media with fortification of 0.5% peptone and 0.3% yeast extract, and 0.5% of yeast extract is only 46 and 19% of that of LB media, respectively. These results showed that kanary by-Product could be a good and cheaper bacterial culture media if small amount of nitrogen source and growth factor were added.
The quantitative analytical method for the bioactive substance, 3-cyano-4-methoxy-N-methyl-2-pyridone (ricinine) and an index compound, ricinoleic acid in castor plant (Ricinus communis) extract or oil was developed. For the determination of a pyridone alkaloid compound, ricinine, successive cartridge cleanup method combined with ultra-performance liquid chromatography was set up with $ENVI-Carb^{TM}$ (0.5 g) and $C_{18}$ SPE cartridges. Accuracy and precision were evaluated through fortification studies of one biopesticide (PE) at 10 and $100mg\;kg^{-1}$. Mean recoveries of ricinine were 98.7 and 96.0 % associated with less than 10 % RSD, respectively. For the determination of ricinoleic acid in castor extract and oil, saponification and methylation were optimized using gas chromatography-time of flight mass spectrometry. Recovery was more than 84.8 % associated with 6.2 % RSD after derivatization procedure. Both methodologies developed were applied to analyze real samples including three castor oil products and six commercially available biopesticides containing R. communis, collected at Korean market. The contents of ricinine and ricinoleic acid in most commercial biopesticides were less than the oil or extract contents indicated by label.
This thesis, deduced from studying eastern and western medical records, deals with geriatric demedtia in modern society. The result were obtained as follows : 1. Dementia is a kind of chronic, progressive, degenerative disease. The chief expression and pathogenic change of the disease is organic: e.g., extensive change such as cerebrum - atrophy, and denaturalization result. in such a situation intellectual capacities and the ability to enjoy daily life deteriorate trenendously. 2. A basic internal cause of the disease is Defficiencies of the heart, liver and kidneys. An exterior cause is an Excessiveness of the 'Dam'(痰), 'Blood Stasis', 'Fung'(風) and 'Fire'. In a Western Medical view, the reason for dementia is due to the onset of Alzheimer's disease and Brain anemia resulting from Multi - infarction or some other reason. If the white - matter of the brain is injured, then dementia easily to results. 3. Disease symptoms result in troubles in intellectual functions : e.g., memory, orientation, intelligence, judgement, common sence and calculating abilities. 4. The proper therapeutic treatment depends on the causes. When the Deficiency is serious, Fortification (heart, liver. and kidney deficiency) is applied and Decrease is follow. When Excessiveness of wrong is serious, the Decrease is tried before the supplement measure is used depending on the deficiency, which generally is used together with 'Fortify Right - Decreace Wrong'. 5. If the disease wasn't caused by some mental reason, it's difficult to be cured of the disease. When the degree of the disease is light and it doesn't continue for a long time, the therapeutic treatment can block the disease's progress and improve the patient's symptoms.
The effect of addition of pectin on the stability of casein solution was studied to apply casein-pectin mixture to apple juice. The solubility of 0.1% casein solution was below 20% at pH 3-5. However, the solubility of 0.1% casein-0.1% pectin mixtures was over 70% at pH 2-10. The increase in the concentration of casein-pectin mixture showed adverse effect on the solubility. The apple juice (pH 3.4), containing 0.1-0.5% casein-pectin mixtures, remained stable without the precipitation of casein. The stability of apple juice including casein-pectin mixture was maintained upon heating at $100^{\circ}C$ for 10 minutes or refrigerating for a week. This study suggested the possibility of protein fortification to acidic beverages since casein-pectin mixture maintained stability in apple juice.
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