• Yonsei Medical Journal
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• 제59권9호
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• pp.1015-1025
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• 2018

Kidney diseases including acute kidney injury and chronic kidney disease are among the largest health issues worldwide. Dialysis and kidney transplantation can replace a significant portion of renal function, however these treatments still have limitations. To overcome these shortcomings, a variety of innovative efforts have been introduced, including cell-based therapies. During the past decades, advances have been made in the stem cell and developmental biology, and tissue engineering. As part of such efforts, studies on renal cell therapy and artificial kidney developments have been conducted, and multiple therapeutic interventions have shown promise in the pre-clinical and clinical settings. More recently, therapeutic cell-secreting secretomes have emerged as a potential alternative to cell-based approaches. This approach involves the use of renotropic factors, such as growth factors and cytokines, that are produced by cells and these factors have shown effectiveness in facilitating kidney function recovery. This review focuses on the renotropic functions of bioactive compounds that provide protective and regenerative effects for kidney tissue repair, based on the available data in the literature.

• Journal of Forest and Environmental Science
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• 제35권4호
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• pp.248-257
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• 2019

We chose the Mt. Dalum area (located in Gijang-gun, Busan, Korea) for our survey, particularly The pine wilt disease zone and the non-permanent control area. This study investigates the effect of pine wilt disease on the distribution of beetle species in the process of ecosystem change due to insect control; pine forests treated for pine wilt disease were divided into insect control and non-control sites, respectively. The results of this study are as follows. Twen tyseven species belongs to 12 families were identified from 969 ground beetles collected from this sites. Species richness was the highest in Coleoptera (6 species, 469 individuals). In the control site, 21 species belongs to 10 families were identified from 228 individuals, while 24 species of 11 families from 533 individuals in the non-control area. The highest number of species were noted in June and July from the non- control and control sites, respectively. The highest number of insects in control and non-control sites was observed in July, while the lowest in September. Sipalinus gigas gigas, Spondylis buprestoides, Plesiophthalmus davidis, Calosoma maximowiczi, Damaster jankowskii jankowskii, and Damaster smaragdinus were captured in both study sites. Episomustur ntus and Glischrochilus ipsoides were only captured in the control site, while Macrodorcas rectus rectus and Pheropsophus javanus were only captured in the non-control site. Six beetles and five species (such as Calosoma maximowiczi) were found in the control site and six species (including Damaster smaragdinus) in the non-control site. The species distributions by altitude were 163, 518, and 258 individuals, at 100, 200 and 300 m sites, respectively. The diversity, evenness, and dominance indices in the control area were 0.764, 0.812, and 0.367, respectively. The diversity, evenness, and dominance indices in the non-control area were 0.927, 0.837, and 0.352, respectively. The similarity index between the control and non-control area was 80%.

• Journal of Ecology and Environment
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• 제38권1호
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• pp.85-93
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• 2015

In 2013, the epidemics of pine wilt disease caused by the pine wood nematodes (Bursaphelenchus xylophilus) resulted in damages to the forests of black pine (Pinus thunbergii Parl.) trees in Jeju Island, Korea. Among the affected trees, an old black pine tree at Cave Temple on Mt. Sanbangsan was included and died due to the prevalence of pine wilt disease. The tree was on Mt. Sanbangsan, which was designated as a National Scenic Place with the Number 77 and was believed to be more than 400 years old in age. By examining the disc of the tree stem obtained from the height of 2 m, we counted the tree rings from 4 different directions and cross-dated the readings by comparing the records of drought simulated from the BROOK Model. Our analysis indicates that the tree seems to have grown since late 1860s. Contrary to the belief of the general public, we can conclude that the age of the tree was estimated to be at maximum 150 years, which means that it was not the same old tree as was shown in the painting of the Tam-Ra-Sun-Ryeok-Do (an old painting book for the Inspection Tour of Jeju Island) published in 1702. Discussion was extended to the life history of the tree in growth and leaning and the measures to protect the tree species from the damages of the pine wilt disease caused by pine wood nematodes.

• Journal of the Korean Wood Science and Technology
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• 제34권2호
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• pp.95-100
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• 2006

This study is to isolate bio-active compounds from Alnus firma and evaluate their antioxidant activity. Dried wood powder of A. firma was extracted by organic solvents and fractionated in the sequential extraction steps. The isolated compounds were characterized by EI-MS, $^{13}C-$ and $^1H-NMR$ including COSY, DEFT, HMQC, and HMBC. Antioxidant activities of the isolated compounds were evaluated by DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging effect. From the wood of A. firma, three kinds of diarylheptanoids, alnusodiol (1), alnusonol (2) and alnusone (3), and gallic acid (4) were isolated. Among these four compounds, compound 1, 2, and 3 are isolated from A. firma for the first time. The antioxidant activity of gallic acid was 93.5% at the concentration of 100 ppm. This compound showed stronger antioxidant activity than those of other isolated compounds and the reference BHT (butylated hydroxytoluene).

• 한국농림기상학회지
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• 제2권2호
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• pp.68-73
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• 2000

• Animal cells and systems
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• 제14권1호
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• pp.31-36
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• 2010

The pinewood nematode Bursaphelenchus xylophilus causes pine wilt disease and is a serious economic concern for the forest industry of South Korea. To achieve effective control with limited resources, it is necessary to clarify the transmission routes and mechanisms of dispersal of this organism. Highly polymorphic and easy-to-use molecular markers can be used for investigating this aspect. In this study, we evaluated the usefulness of amplified fragment length polymorphisms (AFLPs) for investigating the genetic variations of B. xylophilus and related individuals from China, Japan, and South Korea. The AFLP patterns obtained in our study were similar to the microsatellite patterns reported in a previous study; our AFLP patterns indicated high genetic variability and cryptic genetic structure, but did not indicate any peculiar geographic structure. Moreover, the genetic distances between individuals suggested that the Korean population was affected to a greater extent by the Chinese population than the Japanese population. Further, the gene flow among the related species appeared to be limited; however, there may be also the possibility of genetic introgression among species. These results confirm the usefulness of AFLPs for understanding the epidemiology of pine wilt disease, thereby contributing to the effective control of this disease.

• The Plant Pathology Journal
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• 제25권2호
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• pp.121-127
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• 2009

Since July 2005, survey of chestnut ink disease was carried out in chestnut stands located at southern parts of Korea. Dead chestnut trees showing inky ooze on necrotic trunks were found in two different locations. In order to isolate and identify the causal fungus, infected tissues and soil samples around dead or dying trees were collected and placed on Phytophthora-selective medium. Rhododendron and chestnut tree leaves were used as a bait to isolate the fungus from soil samples by attracting zoospores in soil suspensions. On V-8 culture medium, the isolates produced homothallic oogonia with protuberances ($34.0-46.2{\times}21.9-26.7{\mu}m$) abundantly, but did not produced sporangia. Mass production of sporangia was possible by immersing agar plugs with actively growing mycelium in the creek water at $18^{\circ}C$ for 3 days. Sporangia were papillate, and ovoid to obpyriform ($17.0-38.9{\times}14.6-29.2{\mu}m$) in shape. Comparison of the ITS sequences revealed that the isolates had 100% identity to the P. katsurae isolates from Japan and New Zealand and 99.6% identity to other P. katsurae isolates. All of the examined isolates from Korea were completely identical to each other in ITS sequence. Numerous sporangia were formed in filtered as well as unfiltered creek water, but no sporangia formed in sterilized distilled water. Light induced sporangia formation, but has no influence on oospore formation. Amendments of ${\beta}$-sitosterol in culture media have no significant effect on mycelial growth but significantly stimulate oospore and sporangia formation.

• Journal of Forest and Environmental Science
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• 제33권4호
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• pp.342-347
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• 2017

Since the first report of the oak wilt disease at 2004 in Korea, the disease distributed over Korean peninsula and are still giving severe damages. The management of oak wilt disease in Korea has mainly focused on the control of insect vector, Platypus koryoensis. Neverthless the effective method for evaluating the pathogenicity of the pathogen, Raffaelea quercus-mongolicae (Rqm), and for screening chemical or biological agents with strong inhibitory activity against the pathogen, is absolutely necessary, an reliable method is not available so far. This study was conducted to develop the effective method for evaluating the pathogenicity of Rqm in oak trees. The culture suspensions of Rqm were artificially injected to the saplings of Quercus acutissima by using ChemJet tree injector. Three months after treatments, the treated saplings were cut and dipped into 1% fuchsin acid solution. There were significant differences in non-conductive area (%), discoloration area (%) and vertical discoloration length between the pathogen-injected and distilled water-injected control treatments. These results indicated that the pathogen is the causal agent for the dysfunction of water conductive tissue, which will finally result in wilt symptom. Re-isolation of the pathogen and PCR detection using specific primers for the pathogen also confirmed the presence of Rqm in the sapwood chips of the pathogen-injected saplings. These observations would be greatly applied to other related researches for evaluating the pathogenicity of tree wilt pathogens and biocontrol efficacy of the selected antagonistic microorganisms, in case that the wilt symptom is not easily shown by artificial inoculation of the causal agent.

• 한국산림과학회지
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• 제96권6호
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• pp.750-755
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• 2007

본 연구는 2004년-2005년 민가시오갈피나무를 가해하는 오갈피나무이의 생태적 특성을 구명하기 위해 수행되었다. 알의 형태는 촛불모양이며 길이는 $0.40{\pm}0.03mm$이다. 약충은 5령기를 거치며 1령 약충의 체장은 $0.36{\pm}0.03mm$, 종령약충의 체장은 $2.50{\pm}0.25mm$이다. 1년에 2세대를 경과하며, 제 1세대 성충의 우화기간은 7월 중순부터 8윌 상순까지였고, 최성기는 7월 하순 이었다. 제 2세대 성충의 우화기간은 9월 중순부터 10월 상순까지였고, 최성기는 9월 하순 이었다. 월동한 성충은 4월 상순부터 5월 하순까지 월동처에서 오갈피나무의 잎이나 줄기로 이동하기 시작하였고, 이동 최성기는 4월 히순 이었다. 월동성충(♀)은 민가시오갈피나무의 개엽된 잎이나 새로 성장한 줄기를 선호하며, 제 1세대 성충은 종실이 형성되기 시작할 때 주로 종실사이에 산란하나 일부는 가지에도 산란하였다. 성충의 포란수는 $57.5{\pm}23$개 이었다.

• The Plant Pathology Journal
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• 제23권2호
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• pp.70-75
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• 2007

We conducted an inoculation test using nine open-pollinated families of pine trees to evaluate their susceptibility and mortality in different densities of pine wood nematode. Three-year-old nine open-pollinated pine families were inoculated with Bursaphelenchus xylophilus at levels of 3,000, 5,000, and 7,000 nematodes/seedling in greenhouse. There were no distinct patterns in latent period among three densities of B. xylophilus in all families. Most families showed the first disease symptoms of needle discoloration within 12-15 days after inoculation. However, open-pollinated progenies of Pinus densiflora showed the longest latent period because none of one-year-old needles were wilted until 14 days after inoculation with 5,000 and 7,000 nematodes. One-year-old needles were wilted earlier than current needles in all tested families with all densities of B. xylophilus. Current needles were not wilted until 14 days after inoculation in all seedlings. The mortality of all seedlings rapidly increased from 35 days to 49 days after inoculation, and all died within 80 days except two seedlings. A 3,000 nematodes/100 ${\mu}L$ with sterilized distilled water are enough to screen 3-year-old pine seedlings for resistance to B. xylophilus.