• Asian-Australasian Journal of Animal Sciences
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• v.24 no.8
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• pp.1086-1091
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• 2011

This experiment was designed to investigate the effect of lerak extract on the dynamic of rumen microbes in the in vitro fermentation of diet with different ratios of forage and concentrate. In vitro fermentation was conducted according to the method of Tilley and Terry (1963). The design of experiment was a factorial block design with 2 factors. The first factor was the ratio of forage and concentrate (90:10, 80:20, and 70:30 w/w) and the second factor was the level of lerak extract (0, 0.6, and 0.8 mg/ml). Total volatile fatty acid (VFA) concentration, proportional VFA and NH3 concentration were measured at 4 h incubation. Protozoal numbers in the buffered rumen fluid after 4 and 24 h of incubation were counted under a microscope. Bacterial DNAs of buffered rumen fluid were isolated from incubated samples after 24 h of incubation using a QiaAmp kit. Total bacteria, Fibrobacter succinogenes, Ruminococcus albus, and Prevotella ruminicola were quantified using real time polymerase chain reaction (PCR). Lerak extract markedly reduced protozoal numbers in buffered rumen fluid of all diets after 24 h of incubation. Total bacteria did not change with lerak extract addition. While no difference in F. succinogenes was found, there was a slight increase in R. albus number and a significant enhancement in P. ruminicola number by increasing the level of lerak extract in all diets. Propionate concentration significantly increased in the presence of lerak extract at level 0.8 mg/ml. It was concluded that the addition of lerak extract could modify rumen fermentation and had positive effects on rumen microbes.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.3
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• pp.164-168
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• 1995

A field experiment was carried out to determine the effects of nitrogen (N) fertilization levels after first harvest (0, 30, 60, 90 and 120 kglha) during spring season on the gms growth, dry matter (DM) yield, nutritive value, and efficiency of DM production per N kg applied in orchardgrass meadow. Additional N was applicated at early spring (70 kg), after second harvest (50 kg), and after fourth harvest (30 kg/ha) in all treatments, 1990. Growth and DM yield of orchardgrass at second harvest were significantly increased with increasing level of N after first harvest (P<0.05), but no yield differences were observed between application of N 60, N 90, and N 120 kg. The efficiency of DM increment per N kg applied was rapidly decreased with increasing level of N; that was 14.8, 12.0, 10.7 and 4.7 DM kg/N kgha in the plot of N 30, N 60, N 90 and N 120 kg, respectively. The content and yield of crude protein of orchardgrass at second harvest were significantly increased with increasing of N (P<0.05), ranged h r n 11.0% (N 0 kg) to 16.8% (N 120 kg), and from 195.0 kg (N 0 kg) to 508.2 kglha (N 120 kg), respectively. However, the content of neutral detergent fiber was not affected by N level after first harvest. From the above results, it is suggested that application of N fertilizer after first harvest was very effective for regrowth and nutritive value of orchardgrass during spring season, and the proper amount of N after first harvest was 60-90 kglha in this experiment.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.2
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• pp.118-123
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• 1995

This experiment was canied out to determine the effects of nitrogen (N) fertilization level (0, 70, 140, 280 and 420 k a a ) on the grass growth, dry matter (DM) production, numtive value, and botanical composition in reed canarygrass (Phalaris ancndinacea L.) pasture. \ulcornerhe cultivar used in this study was Palaton, and the grass was harvested at soiling-hay stage (3 times per year) in 1992 and 1993. As the N level was increased, the plant height, DM produdion, and crude protein (CP) content were significantly increased (P<0.05). When the N was applied at the levels of 0, 70, 140, 280, 420 kg, DM yields were 4,540, 5,316, 6,060, 7,923 and 9,960 kgha, and CP contents were 17.6, 17.4, 17.4, 18.4 and 19.8%, respectively. However, as the N level was increased, DM percentage, total digestible nutrients, and relative feed value were decreased, while the contents of neutral detergent fiber and acid detergent fiber, and bareland were continuously increased, particularly in the plot of N 420 kg fdzation. From the above results, it is suggested that reed canarygrass was one of the sensitive pasture grasses to N fertilizer, and the proper amount of N application was 280 kg/ha/yr for desirable production, nutritive value, and botanical composition in r e d conarygrass pasmre.

• Journal of The Korean Society of Grassland and Forage Science
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• v.40 no.4
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• pp.279-284
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• 2020

This study aimed to analyze ruminal fermentation, methane emissions, and methanogen levels for different forage feed type and concentrate feed ratios. Alfalfa hay, oat hay, and a feed concentrate were used for in vitro fermentation experiments, at ratios of 9:1, 5:5, and 1:9 (forage:concentrate). After 24 h of incubation, rumen fermentation and methanogen level changes were evaluated. In the low forage treatments, the total gas, CH4, NH3-N, true dry matter digestibility, and total volatile fatty acid were higher than the other treatments, which were used as the parameters on which to assess rumen fermentation (P < 0.05). The feed ratio influenced the copy number for the total archaea and the genus Methanobrevibacter (P = 0.015, P = 0.010). The copy number result trend was like that for CH4 per digested dry matter (DDM). The PCR results and methanogen copy number analysis indicated that the composition of the methanogens affected the CH4 levels, not their copy number. The results of this study can be applied to predict rumen fermentation and methane emission patterns for cattle fed a variety of feedstuffs.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.2
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• pp.104-110
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• 1992

This experiment was carried out to determine the effects of nitrogen(N) fertilization levels(0. 120, 240 and 360 kg/ha/y) on seasonal nitrate nitrogen($NO_3$-N) concentration and water soluble carbohydrate (WSC) content of grasses grown under shading condition. The plants were sampled on 7. 14, 21, 28 and 35 days after harvesting in spring(May), summer(July) and autumn(September), 1988. respectively. Shading degree was controlled artificially ca. 45-50%, and each annual level of N was distributed 5 times equally. The $NO_3$-N concentration of grasses was very low(0.064 %) in spring, high in summer(O.l98 %) and autumn(0.234%), and NO,-N was slightly decreased with growing of grasses. Also the NO,-N concentration of grasses was increased significantly with increasing level of N. In spring, the $NO_3$-N of grasses was very safety(less than 0.1 %), regardless of plant height and N level. In summer and autumn, however, plant heights of below 40 cm(summer) and 44 cm(autumn), and N levels of over 210 kg(summer) and 140 kg(autumn) were over toxic level to animals, respectively. The WSC content of grasses was slightly decreased with grass growth. and significantly decreased with high level of N. From the above results, it is suggested that 200 kg/ha of N in this experiment is effective for forage quality. Also a little more amount of N(50-70kgltimes) in spring. and low level of N(20-30 kgltimes) in summer and autumn season may be desirable on woodland posture.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.3
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• pp.341-352
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• 2012

In large-type goats that were fed on dry forage twice daily, dry forage intake was markedly suppressed after 40 min of feeding had elapsed. The objective of this study was to determine whether or not marked decreases in dry forage intake after 40 min of feeding are mainly caused by the two factors, that is, ruminal distension and increased plasma osmolality induced thirst produced by dry forage feeding. Six large-type male esophageal- and ruminal-fistulated goats (crossbred Japanese Saanen/Nubian, aged 2 to 6 years, weighing $85.1{\pm}4.89kg$) were used in two experiments. The animals were fed ad libitum a diet of roughly crushed alfalfa hay cubes for 2 h from 10:00 to 12:00 am during two experiments. Water was withheld during feeding in both experiments but was available for a period of 30 min after completion of the 2 h feeding period. In experiment 1, saliva lost via the esophageal fistula was replenished by an intraruminal infusion of artificial parotid saliva (RIAPS) in sham feeding conditions (SFC) control, and the treatment was maintained under normal feeding conditions (NFC). In experiment 2, a RIAPS and non-insertion of a balloon (RIAPS-NB) control was conducted in the same manner as the SFC control of experiment 1. The intraruminal infusion of hypertonic solution and insertion of a balloon (RIHS-IB) treatment was carried out simultaneously to reproduce the effects of changing salt content and ruminal distension due to feed entering the rumen. The results of experiment 1 showed that due to the effects of multiple dry forage suppressing factors when feed boluses entered the rumen, eating rates in the NFC treatment decreased (p<0.05) after 40 min of feeding and cumulative dry forage intake for the 2 h feeding period reduced to 43.8% of the SFC control (p<0.01). The results of experiment 2 indicated that due to the two suppressing factors of ruminal distension and increased plasma osmolality induced thirst, eating rates in the RIHS-IB treatment were, as observed under NFC, reduced (p<0.05) and cumulative dry forage intake for the 2 h feeding period decreased to 34.0% of the RIAPS-NB control (p<0.01). The combined effects of ruminal distension and increased plasma osmolality accounted for 77.5% of the suppression of dry forage intake 40 min after the start of dry forage feeding. The results indicate that ruminal distension and increased plasma osmolality induced thirst are the main factors in the suppression of dry forage intake in large-type goats.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.3
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• pp.30-40
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• 1992

The area of grassland established and the arable land in forage production have been decreased recently in Korea. As a result, forages available from the grassland and the arable land provided only 40% of the total roughage consumed by cattle in 1991, compared with 54% in 1989. The decreasing trend of the forage production has been impinged on by a number of legislative. socio-economic. technical and other constraints negatively affecting grassland and forage development. Consequently. effective systems and strategies are quite necessary to have sustainable grassland farming in Korea. In spring and autumn, temperatures are too low for subtropical forage crops, and the summer monsoon climate is another serious limitation to the use of cool-season species. Therefore it is an imperative that not only the research-based grassland development but also the forage crop production should be strongly supported by the government authorities to overcome such climatic limitations. Private forest land holdings are of relatively small units in Korea. Accordingly. it is necessary and important to enlarge the forest land holdings per farm to develop as economic units of grassland. For this the government should introduce new policy measures such as for example: long-term leases of Sorest land and the idle arable land of absentee owners ; rational rental system of national and public forest lands; integrating livestock and forest production. All the laws and regulations standing in the way of the grassland development in forest lands should be modified and revised to open the way for much easier development of grassland. It is also proposed that a high level of financial incentives -hould be provided for structural improvements for the grassland development. They may be: mechanization facilitation, construction of new roads relating to grassland, and land exchange and consolidation. And it is basically necessary that financial enumeration or profits must be garanteed in order to motivate farmers to keep up farming continuously. For more efficient grassland development in mountainous areas, reorganization of Alpine Experiment Station and NAB1 Namwon Branch Station may be needed. Research should be strengthened for completion of pasture mixtures, development of maximum forage production methods with a view to saving labor and reducing production costs, introduction of grazing and forage conservation techniques. and utilization of rice straw as more palatable and nutritious forage source. In order to have more efficient and effective transfer of advanced forage production technologies to livestock farmers the user-clients. it is also essential that special training measures should be given to livestock farmers through making greater use of specialized research and extension workers.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.4
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• pp.247-252
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• 2002

“DK 537”has been selected by forage crop breeding team at the National Livestock Research Institute(NLRI), RDA and has been determined by the Deliberative Council of the National Agricultural Cooperative Federation(NACF) in 2002, as a new recommended hybrid which is early maturing, good quality and hi호 yield of corn for silage. The characteristics of this hybrid are as follows ; 1. The seed coat is yellow and the mean of tasseling date is 5th of July, included in early maturing hybrid which is one day delayed than check cultivar, DK 501. The culm length is 246cm. It is resistant to lodging because of its low height from surface to ear. 2. DK 537 shows resistance to H. maydis and Maize Black-Streaked Dwarf Virus(MBSDV). It also shows strength to corn borer as much as DK 501 does. 3. Fresh yield. dry matter yield. and TDN per a ha are 50 tons, 16.9 tons, and 11.4 tons respectively. which are almost same yielding level of DK 501. Its percent ear to total dry matter is 50.8% at the same time. Through all these tests, we could make sure at DK 537 hybrid will be recommended as good forage crop.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.6
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• pp.553-557
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• 2014

The steady increase in livestock industry has greatly required the stable production of food and forage crops. As an alternative, rice-forage cropping system has been attempted in several southern areas. The present study was performed to understand whether an application of cattle-manure compost affects soil chemical properties and crop productivity in rice-forage cropping system, rice ${\rightarrow}$ summer oat ${\rightarrow}$ rye, in Jangheong county, south Jeolla province from 2013 to 2014. Treatments was composed of control (no compost), CM1 (compost application before rice transplanting), and CM2 (two-times compost application, before rice transplanting and after rice harvest), and inorganic fertilizers (N, P, and K) were equally dressed in all plots. Yields of rice were not significantly different between treatments, however, oat production was 1.25-fold higher in CM1 and CM2. Nutrient uptake amounts of rye were higher in CM2 than CM1 and control. Total nitrogen in soil was maintained stable level during crop cultivation. And soil organic matter contents in all treatments were increased by crop residue. Available P_2O_5$ and exchangeable K were increased by cattle manure application. Therefore, it suggested that the amount of nutrient by forage crop residue should be considered in rice-forage multiple cultivation.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.3
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• pp.422-428
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• 1999

The mineral status of native goats and forage species, namely; Cynodon plectostachyus, Pennisetum purpureum. Eleusine indica, Cynodon dactylon, Calopogonium muconoides, Centrosema pubescens, Leucaena leococephala, and Mimosa pudica in lahar affected areas of Concepcion, Tarlac, Philippines were determined. Forage and blood samples were collected six times in 1996-97, and analyzed for calcium, phosphorus, magnesium, sulfur, copper, iron, molybdenum, selenium, and zinc. Forage calcium and sulfur are non-limiting. Most species had low phosphorus, copper and selenium, while some had magnesium and zinc levels lower than the critical limit because of low mineral content and high percolation rate of lahar deposits. Iron and molybdenum were in excess. The effect of seasonal variation was observed only in copper, sulfur and iron. Average blood mineral concentration of the animals was above critical limit, but there were no significant differences between seasons. All the animals had plasma phosphorus and magnesium above critical level; but 20 % had low copper, zinc and selenium especially in dry season possibly due to insufficient amount of these elements and excessive molybdenum and iron in most forage. Conversely, calcium in forage was high; but 40 % of the animals had low plasma calcium concentration. Although no clinical signs of mineral deficiencies were observed, supplemental feeding would be important since the condition of the pasture in lahar-laden areas is not expected to improve in the next five years. Intensified use of L. leucocephala with better mineral profile would be ideal.