• Korean Journal of Veterinary Research
• /
• v.57 no.3
• /
• pp.197-200
• /
• 2017

This study evaluated the effect of a combination of acetaminophen and vitamin C (CAV) on reducing serum cortisol and tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) concentrations in piglets vaccinated with foot-and-mouth disease (FMD) vaccine. Piglets were vaccinated with FMD vaccine and treated with CAV at concentrations of 0.0, 0.5, 1.0, and 2.0 kg/ton feed (P-CON, AD-1, AD-2, and AD-3, groups, respectively) for 5 days post-vaccination. Cortisol and $TNF-{\alpha}$ levels at 5 days post-treatment in the AD-1-3 groups were significantly lower than that in the P-CON group (p < 0.05). There were no significant differences between AD-2 and AD-3 groups and non-vaccinated, non-CAV-treated piglets.

• Korean Journal of Veterinary Service
• /
• v.42 no.4
• /
• pp.297-300
• /
• 2019

In countries with FMD vaccination, as in Korea, typical clinical signs do not appear, and even in FMD positive cases, it is difficult to isolate the FMDV or obtain whole genome sequence. To overcome this problem, more rapid and simple NGS system is required to control FMD in Korea. FMDV (O/Boeun/ SKR/2017) RNA was extracted and sequenced using Ion Torrent's bench-top sequencer with amplicon panel with optimized bioinformatics pipelines. The whole genome sequencing of raw data generated data of 1,839,864 (mean read length 283 bp) reads comprising a total of 521,641,058 (≥Q20 475,327,721). Compared with FMDV (GenBank accession No. MG983730), the FMDV sequences in this study showed 99.83% nucleotide identity. Further study is needed to identify these differences. In this study, fast and robust methods for benchtop next generation sequencing (NGS) system was developed for analysis of Foot-and-mouth disease virus (FMDV) whole genome sequences.

• Journal of the Korean GEO-environmental Society
• /
• v.15 no.8
• /
• pp.5-11
• /
• 2014

In this study, chemical analysis and ecotoxicity tests of leachate from disposal site for foot-and-mouth disease carcasses (FMD leachate) were conducted to collect fundamental data that will be used to develop environmental risk assessment tools for FMD leachate. For chemical analysis, concentration of $Cl^-$, $NH{_4}{^+}-N$, Korea standard method indicators for detection of leachate released from animal carcasses burial site into groundwater and NRN (Ninhydrin-Reactive Nitrogens), a newly suggested screening test indicator to detect groundwater contamination by FMD leachate, were assessed. For ecotoxicity tests, luminescent bacteria (V. fischeri), micro-algae (P. subcapitata) and water flea (D. magna) were selected as test species. Correlation analysis between the concentration of $Cl^-$, $NH{_4}{^+}-N$, NRN and the toxicity to V. fischeri was performed to identify the better indicators to monitor FMD leachate contamination. From regression analysis, the concentration of the indicators in FMD leachate contaminated sample that induced halfmaximal toxic effect to V. fischeri was evaluated. Results obtained from this study can be applied to assess the risk by FMD leachate and to establish the guideline to manage risk in relation to FMD leachate.

• Journal of Veterinary Clinics
• /
• v.28 no.1
• /
• pp.113-121
• /
• 2011

Foot-and-mouth disease (FMD) is a severe vesicular disease of cloven-hoofed animals including domesticated ruminants and pigs. Acute clinical signs may be mild in sheep and goats but are associated with lameness in pigs and mouth lesions with vesicles in cattle. The required condition for a successful pathogen appears to be the ability to counteract both the host innate and adaptive immune response. FMD virus (FMDV) inhibits the induction of antiviral molecules and interferes with the secretory pathway in the infected cell. The surface expression of Major Histocompatibility Complex (MHC) class I molecules is reduced in infected cells. Thus, the ability of the host to recognize and eliminate virus infected cells is decreased. Furthermore, FMDV infection results in a rapid, but transient lymphopenia, reducing the number of T and B cells, and affecting T cell function. The virus appears to premature apoptosis-mediated cell death because it has a very short replication cycle and is able to rapidly produce large amounts of virus. FMDV engages the host protective response at multiple steps to ensure its effective replication and pathogenesis. This review describes the recent pathological and immunological studies to overcome the powerful abilities of FMDV to counteract defense mechanism of host.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.20 no.10
• /
• pp.425-432
• /
• 2019

This study examined the effects of livestock disease news, such as foot-and-mouth disease (FMD), collected by the Korea Press Foundation on BIGKinds on the consumption of beef and pork. The consumer purchase data were obtained from the Rural Development Administration's Agrifood consumer panel data. Survival analysis showed that the FMD news noted beef and pork, and the first purchase rate decreased due to FMD news. The time-dependent Cox proportional hazards model revealed all models to be significant. FMD news was significant in each model. In 2010, the Hazard Ratio of domestic and imported pork was 0.999, and the first purchase rate decreased 0.999 times for each additional FMD news item. On the other hand, the Hazard Ratio of pork in 2015 was 1.001, and the first purchase rate increased. In the case of beef, results similar to pork were shown. This study estimated the impact of consumer meat consumption disease news on the first purchases according to origin and meat. These results provide the necessary information for rational production and consumption activities. In addition, it can also be used for promotion by estimating the duration of livestock disease news.

• Korean Journal of Agricultural Science
• /
• v.45 no.4
• /
• pp.725-732
• /
• 2018

The vaccination for foot-and-mouth disease (FMD) is an effective way to control FMD. However, the injection of FMD vaccine causes abnormalities in pork meat by the incidence of lesions at the injection site. This study was conducted to investigate the inhibition effects of dietary bromelain, a natural protease derived from pineapple stems, on the incidence of lesions at the vaccination site on pigs. A total of 335 pigs (LYD [Landrace ${\times}$ Yorkshire ${\times}$ Duroc]; 7-week-old) were randomly allotted to two dietary treatments: control (basic diet) and bromelain treatment (diet supplemented with bromelain 1 kg/ton). The injection of FMD vaccine was conducted on 56- and 84-day-old pigs. Pigs with the bromelain treatment were fed a diet supplemented with bromelain for 14 days from 5 days before the vaccine injection. After slaughtering the pigs, the number of carcasses that had abnormal meat at the injection site of the vaccine and the amount of abnormal meat, discarded meat, and trimmings were recorded. Pork from the bromelain treated pigs had a lower incidence of abnormal meat caused by vaccine injection as well as a lower amount of abnormal meat, discarded meat, and trimmings than those of the control (p < 0.05). Our result suggests that dietary bromelain could improve the quality of pork meat by inhibiting incidence of lesions at the vaccine injection site.

• Journal of the Korean association of regional geographers
• /
• v.18 no.4
• /
• pp.464-472
• /
• 2012

To assess the space-time clustering of FMD(Foot-and-Mouth Disease) epidemic occurred in Korea between November 2010 to April 2011, geographical information system (GIS)-based spatial analysis technique was used. Farm address and geographic data obtained from a commercial portal site were integrated into GIS software, which we used to map out the color-shading geographic features of the outbreaks through a process called thematic mapping, and to produce a visual representation of the relationship between epidemic course and time throughout the country. FMD cases reported in northern area of Gyounggi province were clustered in space and time within small geographic areas due to the environmental characteristics which livestock population density is high enough to ease transmit FMD virus to the neighboring farm, whereas FMD cases were clustered in space but not in time for southern and eastern area of Gyounggi province. When analyzing the data for 7-day interval, the mean radius of the spatial-time clustering was 25km with minimum 5.4km and maximum 74km. In addition, the radius of clustering was relatively small in the early stage of FMD epidemic, but the size was geographically expanded over the epidemic course. Prior to implementing control measures during the outbreak period, assessment of geographic units potentially affected and identification of risky areas which are subsequently be targeted for specific intervention measures is recommended.

• The Journal of Engineering Geology
• /
• v.23 no.4
• /
• pp.427-434
• /
• 2013

The leachate from landfill (Andong city) contaminated by foot-and-mouth disease (FMD) contains 44%-50% hydrophilic organic matter, compared with 22%-27% in natural water bodies such as ground water, lake water, and river water. In such natural water, the organic matter content is reduced by the metabolism of microbes in the water. However, in the case of leachate-1 and -2, the concentrations of RTOC (refractory total organic carbon) and RDOC (refractory dissolved organic carbon) were higher than the initial TOC and DOC after burial. According to time elapsed after burial, the concentrations of RTOC and RDOC were decreased below the initial TOC and DOC. In the case of leachate-6 (386 days after burial), RDOC made up 91% of RTOC. This result shows that organic matter in the leachate was composed dominantly of RDOM, most of which was not removed by the metabolism of microbes. Hence, the presence and characteristics of RDOM provide a valuable indication of the effect of leachate on the quality of surface water and ground water. Such information is useful in understanding leachate environments.

• Korean Journal of Veterinary Service
• /
• v.43 no.2
• /
• pp.107-112
• /
• 2020

Foot-and-mouth disease (FMD) and avian influenza (AI) are highly pathogenic viral disease which affects the livestock industry worldwide. Outbreak of these viruses causes great impact in the livestock industry; thus, disease infected animals were immediately disposed. Burial is the commonly used disposal method for deceased animals. However, there is potential for secondary environmental contamination, as well as the risk that infectious agents persisting in the environment due to the limited environmental controls in livestock burial sites during the decomposition of the carcasses. Therefore, this study aimed to investigate the detection of FMD and AI viruses from animal carcass disposal sites using real-time reverse transcription PCR. Soil samples of more than three years post-burial from livestock carcass disposal sites were collected and processed RNA isolation using a commercial extraction kit. The isolated RNA of the samples was used for the detection of FMDV and AIV using qRT-PCR. Based on the qPCR assay result, no viral particle was detected in the soil samples collected from the animal disposal sites. This indicates that 3 years of burial and their carcass disposal method is efficient for the control or at least reduction of spread infections in the surrounding environment.

• Journal of Veterinary Science
• /
• v.24 no.1
• /
• pp.15.1-15.13
• /
• 2023

Background: Inactivated vaccines are limited in preventing foot-and-mouth disease (FMD) due to safety problems. Recombinant virus-like particles (VLPs) are an excellent candidate for a novel vaccine for preventing FMD, given that VLPs have similar immunogenicity as natural viruses and are replication- and infection-incompetent. Objectives: The 3C protease and P1 polyprotein of type O FMD virus (FDMV) was expressed in yeast Hansenula polymorpha to generate self-resembling VLPs, and the potential of recombinant VLPs as an FMD vaccine was evaluated. Methods: BALB/c mice were immunized with recombinant purified VLPs using CpG oligodeoxynucleotide and aluminum hydroxide gel as an adjuvant. Cytokines and lymphocytes from serum and spleen were analyzed by enzyme-linked immunosorbent assay, enzyme-linked immunospot assay, and flow cytometry. Results: The VLPs of FMD were purified successfully from yeast protein with a diameter of approximately 25 nm. The immunization of mice showed that animals produced high levels of FMDV antibodies and a higher level of antibodies for a longer time. In addition, higher levels of interferon-γ and CD4⁺ T cells were observed in mice immunized with VLPs. Conclusions: The expression of VLPs of FMD in H. polymorpha provides a novel strategy for the generation of the FMDV vaccine.