• Title/Summary/Keyword: Food component

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Purification and Crystallization of the Recombinant Catalytic Subunit of Pyruvate Dehydrogenase Phosphatase (Pyruvate Dehydrogenase Phosphatase의 Catalytic Subunit의 분리정제 및 결정화)

  • Kim, Young-Mi
    • Journal of Food Hygiene and Safety
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    • v.18 no.3
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    • pp.146-152
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    • 2003
  • Pyruvate dehydrogenase phosphatase (PDP) is a mitochondrial protein serine/threonine phosphatase that catalyzes the dephosphorylation and concomitant reactivation of the pyruvate dehydrogenase component of the pyruvate dehydrogenase complex (PDC). PDP consists of a catalytic subunit (PDPc, Mr 52,600) and regulatory subunit (PDPr, Mr 95,600). In the presence of $Ca^{2+}$, PDPc binds to the dihydrolipoamide acetyltransferase (E2) component of the pyruvate dehydrogenase complex in proximity to its substrate, the phosphorylated E1 component, thereby increasing the rate of dephosphorylation. PDPc possesses and intrinsic $Ca^{2+}$ binding site and a second $Ca^{2+}$ site is generated in the presence of E2. Using the unique interaction, highly pure PDPc was produced by the GSH-Sepharose-GST-L2 matrix with a specific activity of approx. 1000 U/mg and a yield of about 80%.

Methods for Identification of Irradiated Foods (방사선 조사식품의 검출기법)

  • 양재승
    • Journal of Food Hygiene and Safety
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    • v.12 no.2
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    • pp.160-174
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    • 1997
  • Progress in commercialization of the irradiation process, greater international trade in irradiated food, differing regulations relating to use of the technology in many countries, and consumer demand for clear labeling of irradiated food highlighted the need for tests that could be applied to the food itself. Scientists have had to focus on identifying and isolating the minute changes caused in the component food molecules by the process. A number of investigators have reviewed the changes occurring in food after irradiation, detecting and measuring the effects of irradiation. The Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture organised a coordinated program on analytical detection methods in irradiation treatment of food (ADMIT) which promoted cooperation in this area and sponsored collaborative testing of some of the most promising methods.

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Food Ingredients That Inhibit Cholesterol Absorption

  • Jesch, Elliot D.;Carr, Timothy P.
    • Preventive Nutrition and Food Science
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    • v.22 no.2
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    • pp.67-80
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    • 2017
  • Cholesterol is a vital component of the human body. It stabilizes cell membranes and is the precursor of bile acids, vitamin D and steroid hormones. However, cholesterol accumulation in the bloodstream (hypercholesterolemia) can cause atherosclerotic plaques within artery walls, leading to heart attacks and strokes. The efficiency of cholesterol absorption in the small intestine is of great interest because human and animal studies have linked cholesterol absorption with plasma concentration of total and low density lipoprotein cholesterol. Cholesterol absorption is highly regulated and influenced by particular compounds in the food supply. Therefore, it is desirable to learn more about natural food components that inhibit cholesterol absorption so that food ingredients and dietary supplements can be developed for consumers who wish to manage their plasma cholesterol levels by non-pharmacological means. Food components thus far identified as inhibitors of cholesterol absorption include phytosterols, soluble fibers, phospholipids, and stearic acid.

Systematic Evaluation Effects of Culture on the Effectiveness of Small and Medium Firms in Tra Vinh of Vietnam: With AHP and Regression

  • HUYNH, Quang Linh
    • The Journal of Asian Finance, Economics and Business
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    • v.9 no.8
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    • pp.135-144
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    • 2022
  • Organizational culture has been recognized as a determinant of corporate Effectiveness. Cultural components could affect corporate Effectiveness to different extents. However, it seems that none of the previous studies have evaluated and compared these influential extents of cultural components. The current research applies analytic hierarchy processes to assess and rate the significance extents that, cultural components play in resulting in corporate Effectiveness. Then, the current work utilizes regression analyses to re-evaluate the significance extents for the robustness of analytic hierarchy processes. The findings indicate that clan culture is the most vital component in explaining corporate Effectiveness. In contrast, adhocracy culture is the least important component in affecting corporate Effectiveness. Market culture is a cultural component that is ranked the second in determining corporate Effectiveness, and next is hierarchy culture which is the third. The findings are in support of the positive influences of clan, market, and adhocracy cultures on corporate Effectiveness; whereas it found the negative impact of hierarchy culture on corporate Effectiveness.

A System Analysis and Simulation of the Korean Dairy Industry Sector -Milk Supply Component-

  • Lee, Buk-Won
    • Journal of the Korean Operations Research and Management Science Society
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    • v.4 no.1
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    • pp.87-102
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    • 1979
  • In Korea, the traditional main food is rice There is a shortage of food, though the land is uesd intensively. The Koreans produce mainly rice its yield is very much higher than other crops. This leads to the comsumption of one type of food and, hence, to an unbalanced diet. Since 1962, Korean income has been going up rapidly, and the demand for animal protein has also increased. In order to solve the probem, the government decided to develop the dairy industry, because this would enable the population to consume more animal protein, and there is weak competion between rice and milk production.

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Application of Principal Component Analysis to Shelf-Life Determination of Processed Food (주성분분석을 이용한 식품의 저장중 품질변화 평가)

  • Kim, Jung-Hoan;Yoon, Sang-Gi;Choi, Jun-Bong;Kim, Jae-Cherl;Kong, Un-Young
    • Korean Journal of Food Science and Technology
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    • v.27 no.5
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    • pp.703-707
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    • 1995
  • Shelf-life dating of cooked and heat sterilized food was carried out with the use of principal component analysis (PCA). Changes in color, pH, acidity and sensory properties were measured and analyzed during storage at 20, 30 and $40^{\circ}C$. Acceptability of sample was decreased during storage, shelf-life of sample was determined 1.5, 3 and 3 months at 20, 30 and $40^{\circ}C$, respectively. Application of PCA to quality evaluation, principal component (PC) 1 dominated 49.6% of total variation and PC 2 expressed 28.8%. The rate of change of PC 1 to storage time was 1.3 with increasing temperature of $10^{\circ}C$, and close to shelf-life determined by accepability at 20 and $40^{\circ}C$. Therefore, PCA was applicable to evaluate the quality, predict the shelf-life and investigate the quality parameter of food during storage.

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Characteristic study on the chemical components of Korean curved ginseng products

  • Cho, Chang-Won;Kim, Young-Chan;Kang, Jin-Hee;Rhee, Young Kyoung;Choi, Sang Yoon;Kim, Kyung-Tack;Lee, Young-Chul;Hong, Hee-Do
    • Journal of Ginseng Research
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    • v.37 no.3
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    • pp.349-354
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    • 2013
  • Dried ginseng (DG) is in fact the representing ginseng product in the worldwide market. Although it is made in various packages depending on the processing method, size and age of DG, basic scientific data reporting the chemical components are limited. In this study, 4-year-old curved ginseng (CG), one of the domestic DG products, was selected for further investigation. Eighty-six samples of 30 and 50 piece-grade CG, which are the most widely distributed in the market, were collected for 5 yr. Their major components, such as moisture, total sugar, acidic polysaccharides, total phenolic compounds, and saponins, were analyzed to figure out the standard quality characteristics. The moisture content of all CG samples was less than 15%. The total water-soluble sugar contents were 22.9% to 47.8% and 23.2% to 49.5% in the 30 and 50 piece-grade CG, respectively. The acidic polysaccharide contents were 3.6% to 6.7% and 2.9% to 6.9% in the 30 and 50 piece-grade CG, respectively. The total phenolic compound content was 0.4% to 0.5% in CG, regardless of the piece-grade. The crude saponin content, which represents the active component of ginseng, was over 2% in all samples. In 30 piece-grade CG samples, the contents of major ginsenosides, Rb1, Rf, and Rg1, were 2.2 to 4.7 mg/g, 0.4 to 1.3 mg/g, and 1.6 to 4.0 mg/g, respectively. The ginsenoside contents in 50 piece-grade CG samples were 2.1 to 3.9 mg/g (Rb1), 0.5 to 1.2 mg/g (Rf), and 1.3 to 3.4 mg/g (Rg1). Overall, since there were relatively high standard deviation and coefficient of variation in all the chemical component contents that were assessed, we found some difficulties in showing the CG standard chemical component characteristics by average, standard deviation, and other statistical analysis factors.

Protein and Amino-acid Contents in Backtae, Seoritae, Huktae, and Seomoktae Soybeans with Different Cooking Methods (콩의 종류 및 조리방법에 따른 단백질·아미노산 함량 변화)

  • Im, Jeong Yeon;Kim, Sang-Cheon;Kim, Sena;Choi, Youngmin;Yang, Mi Ran;Cho, In Hee;Kim, Haeng Ran
    • Korean journal of food and cookery science
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    • v.32 no.5
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    • pp.567-574
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    • 2016
  • Purpose: The objective of this study was to provide nutritional information (protein and amino-acid contents) of soybeans (Baktae, Seoritae, Huktae, and Seomoktae) with different cooking methods. Methods: Raw, boiled (in $100{\pm}15^{\circ}C$ of water for 4 hr), and fried (in a pan at $110{\pm}15^{\circ}C$ for $20{\pm}5min$) soybean samples were prepared. Contents of protein and amino acids were determined. Results: Protein content in raw Baktae, Seoritae, Huktae, and Seomoktae soybeans ranged from 361.0 to 386.8 mg/g. Protein contents differed according to cooking methods. They were higher in pan-fried beans (107.9-113.5%) than in raw or boiled soybeans (48.2-49.5%). A total of 18 amino acids were analyzed. Amino acid data sets were subjected to principle component analysis (PCA) to understand their differences according to soybean types and cooking methods. Bean samples could be distinguished better according to cooking method in comparison with bean types by principle component (PC1) and PC2. In particular, fried soybeans contained much higher levels of cystein. Other amino acids were the dominant in raw and boiled ones. On the other hand, the amounts of threonine, histidine, proline, arginine, tyrosine, lysine, tryptophan, and methionine were higher in raw bean samples than in cooked ones. Conclusion: The contents of amino-acids and proteins are more effected by different cooking methods in comparison with soybean types.