• 한국식품과학회지
• /
• 제22권6호
• /
• pp.711-715
• /
• 1990

식품 및 의약품 원료로 이용되고 있는 Iysozyme을 난백으로부터 이온교환 크로마토그래프를 이용하여 연속추출하였다. 사용된 수지는 지금까지 수율, 순도, 작동용이성에서 가장 효과적이라고 밝혀져 있는 Duolite C-464 양이온교환수지를 선택하여 $Na^+$형으로 $pH\;7.9{\pm}0.1$로 평형화하여 사용하였다. 조제용 자동 Liquid Chromatography(LC) system(column size ; i.d. 50 mm, bed volumn ; 1020 ml)에서 수지 평형화, 난백접촉, rinse, lysozyme용출 순으로 cycle을 연속적으로 반복하면서 시행하고 그 재현성을 관찰하기 위해 각 cycle별 rinse끝점과 용출끝점의 UV level을 비교하였다. 그 결과 rinse끝점은 19 cycle까지 30% 이하를 유지하였다. 용출 끝점은 17 cycle까지는 30% 이하에서 비교적 안정하였으나 18 cycle 이후 부터는 50% 이상으로 용출능력이 현저하게 감소하였다. 또한, 회수율 비교에서 17cycle까지는 90% 이상을 유지하다가 18cycle에서는 72%, 19 cycle에서는 65%로 격감하였다. 본 추출 정제 lysozyme은 전기영동상 단일 band로 나타났고, densitometer로 측정한 순도는 99% 이상이었다.

• Archives of Pharmacal Research
• /
• 제21권3호
• /
• pp.291-297
• /
• 1998

Cop protein has been overexpressed in Escherichia coli using a T7 RNA polymerase system. Purification to apparent homogeneity was achieved by the sequential chromatography on ion exchange, affinity chromatography, and reverse phase high performance liquid chromatography system. The molecular weight of the purified Cop was estimated as 6.1 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). But the molecular mass of the native state Cop was shown to be 19 kDa by an analytical high performance size exclusion chromatography, suggesting a trimer-like structure in 50 mM Tris-HCI buffer (pH 7.5) containing 100 mM NaCl. Cop protein Was calculated to contain $39.1% {\alpha}-helix, 16.8% {\beta}-sheet$, 17.4% turn, and 26.8% random structure. The DNA binding property of Cop protein expressed in E. coli Was preserved during the expression and purification process. The isoelectric point of Cop was determined to be 9.0. The results of amino acid composition analysis and N-terminal amino acid sequencing of Cop showed that it has the same amino acid composition and N-terminal amino acid sequence as those deduced from its DNA sequence analysis, except for the partial removal of N-terminal methionine residue by methionyl-aminopeptidase in E. coli.

• 동아시아경상학회지
• /
• 제8권4호
• /
• pp.19-28
• /
• 2020

Purpose - This study aims to lay the foundation for a desirable direction to solve future environmental problems through framework research on environmental cooperation according to distribution of urbanization between South and North Korea. The article also attempts to explore solution to two Koreas' environmental problems from the cooperative perspective between South and North Korea and draw future policy tasks. Research design, data, and methodology - For methodology, North Korea's legal system to cope with disasters is taken into consideration in terms of literature review. This study also analyzes a series of processes related to North Korea's disaster management through case study, and draws policy measures for North and South Korea's cooperation scope and methods. Result - The results support that North Korea is very vulnerable to environmental disasters due to food shortages, economic sanctions, and enormous natural disaster damages including flood damages occurring each year, because of the lack of disaster prevention infrastructure such as river maintenance. Conclusion - At the current time when North and South Koran exchange and cooperation increase, a disaster management cooperative system is needed for the areas where South and North Korea manage through division. It also suggests that there is a need to establish regulations and procedures for support items in advance for a quick response to disasters in North Korean region.

• Preventive Nutrition and Food Science
• /
• 제14권3호
• /
• pp.173-178
• /
• 2009

This study was conducted to determine if supplementation with a compound composed of caffeine (50 mg), capsaicin (75 mg), sesamine (30 mg), L-carnitine (300 mg), banaba (50 mg) and lotus (10 mg) enhanced human autonomic nervous activities (ANS) associated with thermogenic sympathetic activity and fat utilization. Ten healthy college males (21.2$\pm$1.0 yr) volunteered for this experiment. Autonomic nervous activities associated with energy metabolism were examined at 30 min intervals for a total of 120-min while at rest and every 5-min during exercise at 50% of the ventilation threshold before and after intake of the compound or placebo with 100 ml of water for 10 days. In addition, heart rate variability power spectral analysis was used to assess human autonomic nervous activities. The results indicated that there were no significant differences in heart rate during rest and exercise among trials. Furthermore, the autonomic nervous activity tended to increase after 10-days of consumption of the test compounds during the experimental period, but the differences did not reach statistical significance. However, before and after the compound test trial there was a significantly higher respiratory gas exchange ratio (rest 0: 0.83$\pm$0.01 vs. rest 3: 0.89$\pm$0.02, p<0.05), carbohydrate oxidation (CHO) rate (rest 0: 44.57$\pm$5.83 vs. rest 2: 63.86$\pm$5.91%, p<0.05) and a lower fat oxidation rate (rest 0: 55.43$\pm$5.83 vs. rest 2: 36.14$\pm$5.91%, p<0.05. In conclusion, the results of the present study suggested that the compound composed of caffeine, capsaicin, sesamine, L-carnitine, banaba and lotus components that was evaluated in this study did not induce a significant increase in human autonomic nervous activities or lipolysis, even though the individual components have been reported to induce increased fat oxidation.

• 한국축산식품학회지
• /
• 제21권4호
• /
• pp.367-373
• /
• 2001

MFGM 당단백질의 하나인 PAS-7을 GPC 및 affinity chromatography에 의해 정제하여 2-AB로 형광 표식한 후, anion-exchange column 및 reversed-phase column을 이용해 5개의 성분을 분리하였다. 그 중 가장 상대량이 많은 성분 e에 대하여 RAAM2000을 이용한 당쇄구조 해석을 실시하여, 성분 e는 RAAM2000 GPC에 의하여 4개의 성분으로 분리되어 각각 calibration standard 12.10, 8.88, 5.84 및 4.86GU의 용출위치에 검출되었다. 이 용출위치와 당쇄구조는 livrary의 component-7457과 일치하며, 12.2GU의 크기로 분자량은 1788로 판단되며 library의 당쇄와 약 85%의 확률로 일치했다. 그 결과 성분 e의 당쇄구조는 환원말단에 $\alpha$1-6결합된 fucose를 1개 함유하며, core부분의 비환원말단에 N-acetyllactosamine branch를 2개 함유한 전형적인 biantennary 당쇄구조인 것으로 추축되어, 이전 HPLC, acetolysis, sequential exoglycosidase 소화, NMR분석에 의해 보고된 성분 7N1A의 구조와 일치함으로써, OGS RAAM2000을 이용한 PAS-7의 당쇄구조 해석의 유용성이 증명되었다.

• Journal of Microbiology and Biotechnology
• /
• 제2권3호
• /
• pp.189-196
• /
• 1992

A $\alpha$-l, 4-D-glucan maltohydrolase $(\beta$-amylase), secreted by the mesophilic aerobic bacterium Bacillus polymyxa No.26, was purified and characterized. The enzyme production was increased after a logarithmic phase of bacterial growth and paralleled with the onset of bacterial sporulation. By applying anion exchange chromatography and gel filtration the enzyme was purified 16.7-fold and had a specific activity of 285.7 units/mg. Two enzyme activities were eluted on a column of DEAE-Sephadex chromatography, and they were designated as E-I for a major enzyme peak and E-II for a minor peak. Of them, E-I enzyme peak was further purified by using gel chromatography. The molecular mass of this enzyme was determined to be 64, 000 daltons and consisted of a single subunit, showing an isoelectric point of 8.9. The enzyme was able to attack specifically the $\alpha$-l, 4-glycosidic linkages in soluble starch and caused its complete hydrolysis to maltose and $\beta$-limited dextrin. This amylolytic enzyme displayed a temperature optimum at $45^\circ{C}$ and a pH optimum at 7.0. The amino acid composition of the purified enzyme was quite similar to the other bacterial $\beta$-amylases reported. Surprisingly, the purified enzyme from this aerobe only exhibited hydrolytic activity on soluble starch, not on starch granules. The degradation of from starch by $\beta$-amylase was greatly stimulated by pullulanase addition. These results differentiated from other $\beta$-amylases reported. Based on a previous result that showed the enzyme system involves in effective degradation of raw starch granules, this result strongly suggested that the purified enzyme (E-I) can be a synergistic part of starch granule-digestion and E-II plays a crucial role in digestion of starch granules.

• 한국포장학회지
• /
• 제9권1호
• /
• pp.33-54
• /
• 2003

The aim of this study was to develop a model that could be used in the design of modified atmosphere packaging (MAP) for peaches. Respiratory data at 5, 10, $20^{\circ}C$ for peaches were gathered and altered for create useful respiration model. Packaging materials were conventional low density polyethylene and polypropylene with anti-fog, and anti-fungi treatments, and thickness was $30{\mu}m$ and $50{\mu}m$ each. Permeability tests were performed to find their oxygen, carbon dioxide, water vapor transmission rate as increases in temperature. Test results were then converted to logarithm format for MAP modeling. The maximum rate of oxygen uptake increased with increasing temperature. Optimum gas composition in the package system for fruits were set according to literature and upper or lower limits of oxygen and dioxide established. To predict gas composition at certain storage time, weight of fruits, film thickness, film type, and other variables, respiration rate was studied at various storage conditions. The results of tests were used to calculate Cameron's model and converted to a cubic estimation equation. The validity of the model was tested experimentally by observing actual atmospheric changes inside packages. This result of study may be useful for designing dynamic gas exchange MAP systems for similar agricultural products.

• 한국패션뷰티학회지
• /
• 제4권1호
• /
• pp.4-11
• /
• 2006

Masstige goods aimed consumers who want the fame and the emotional contents with reasonable price are presented overall and around the life style, from all the fashion items like bag and apparel to car, electric household, food, sports goods, furniture, toys, pets and performance of art, etc. Masstige casual, essentially different from the passed casuals which emphasized only price strategy, appeals to teenagers and young of twenties with a definite brand concept. Therefore masstige casual might be separated from business casual of a target aged thirties. Established celebrity brands have launched masstige brands matching the popularization of prestige goods. Armani Exchange from Armani, Marc by Marc Jacobs from Louis Vuitton are representative ones. DKNY from Donna Karen, MiuMiu from Prada, Paul smith Pink from Paul Smith can be added. These are relatively inexpensive, however the quality, design and shop's atmosphere are more exclusive than general brands. Consumers are over middle class and have a pride and fidelity to those brands. Leading Masstige trend, new luxury brands put the importance to the quality and aims middle class. To succeed in this field, companies should know exactly what consumers want, considering not only functional aspect but also emotional pleasure. Even though masstige has a weakness in pricing, it has to keep brand's proper benefit. Its price range could be wide to be in great demand but has to have elasticity and not to be expanded too much. Masstige industry should do its best not to damage original brand's identity. Forming family brand, like Armani made Georgic Armani, Emporio Armani and Armani exchange, system of parent brand and sub brands would be recommendable. From the launching time, masstige needs the effects to create a sensation and bring it into vogue and offer emotional value to the consumers.

• 지능정보연구
• /
• 제27권4호
• /
• pp.23-48
• /
• 2021

한국경제는 지난 수십년간 정부의 수출전략정책에 힘입어 지속적으로 경제 성장을 이룩해왔으며, 수출의 증가는 경제의 효율성 향상, 고용창출, 기술개발 촉진 등 우리나라의 경제 성장을 견인하는 주도적인 역할을 해왔다. 전통적으로 우리나라 수출에 영향을 미치는 주요 요인은 크게 경제적 요인과 산업구조적 요인이라는 두가지 관점에서 찾아볼 수 있다. 첫번째, 경제적 요인은 환율과 글로벌 경기 변동과 관련된 것으로서, 환율이 우리나라 수출에 미치는 영향은 환율 수준 및 환율 변동성에 따른 영향으로 나누어 살펴볼 수 있으며, 글로벌 경기 변동은 세계 수입 수요에 영향을 미쳐 우리나라 수출을 좌우하는 절대적 요인으로 볼 수 있다. 두 번째, 산업구조적 요인은 국제 분업화의 둔화, 중국의 특정 수입품 자국내 대체 증가, 수출 주력 산업의 해외생산 형태 변화 등 산업이나 제품에 따라 발생한 고유한 특징이다. 가장 최근 글로벌 교류와 관련된 연구들을 살펴보면, 경제적 요인 및 산업구조적 요인과 더불어 문화적인 측면이 중요함을 여러 문헌에서 피력하고 있다. 이에 따라 본 연구에서는 각국의 한국 수입액 예측 모형에 문화적 요인을 함께 반영하여 예측 모형을 개발하고자 하였으며, 구체적으로 문화적 요인이 수입액에 미치는 영향을 PUSH-PULL 프레임워크 관점에서 반영해보고자 하였다. PUSH 관점은 한국이 자신의 브랜드를 개발하고 적극 홍보하는 관점으로 K-POP, K-FOOD, K-CULTURE 등으로 대표되는 한국의 브랜드에 대한 각국의 관심 정도로 정의할 수 있다. 또한, PULL 관점은 각 국가의 국민들의 문화적, 심리적 특징으로 해당 국가의 지배체계, 남성성, 위험 회피성, 시간에 대한 단기/장기 지향성 등으로 대표되는 각 국의 문화 코드로서 한류문화를 얼마나 수용할 성향을 띄고 있는지로 정의할 수 있다. 본 연구에서 제시한 최종 예측 모델의 고유한 특징은 Design Principle에 기반하여 설계한 것인데, 1) 신규로 추가한 데이터 소스를 통해 한국에 대한 관심 및 문화적 특성이 반영될 수 있는 모형으로 구축하였고, 2) 경제적 요인 등의 변화와 품목 및 국가 Code를 입력하면 예측값을 바로 불러올 수 있도록 실용적으로 편의성 있게 설계하였으며, 3) 이론적으로도 의미 있는 결과를 도출하기 위해서 입력과 목표 변수간의 관계를 해석 가능한 알고리즘을 중심으로 설계하였다는 점이다. 본 연구는 기술적 측면, 경제적 측면, 정책적 측면에서 의미 있는 시사점을 제시할 수 있으며, 수입액 예측 모형을 활용하여 중소·중견기업의 수출 지원 전략에 의미 있는 기여를 할 수 있을 것으로 기대된다.

• Journal of Microbiology and Biotechnology
• /
• 제19권9호
• /
• pp.987-996
• /
• 2009

Aspergillus niger GH1 previously isolated and identified by our group as a wild tannase producer was grown under solid-state (SSC) and submerged culture (SmC) conditions to select the enzyme production system. For tannase purification, extracellular tannase was produced under SSC using polyurethane foam as the inert support. Tannase was purified to apparent homogeneity by ultrafiltration, anion-exchange chromatography, and gel filtration that led to a purified enzyme with a specific activity of 238.14 IU/mg protein with a final yield of 0.3% and a purification fold of 46. Three bands were found on the SDS-PAG with molecular masses of 50, 75, and 100 kDa. PI of 3.5 and 7.1% N-glycosylation were noted. Temperature and pH optima were 600e and 6.0 [methyl 3,4,5-trihydroxybenzoate (MTB) as substrate], respectively. Tannase was found with a $K_M$ value of $0.41{\times}10^{-4}M$ and the value of $V_{max}$ was $11.03{\mu}$moL/min at $60^{\circ}C$ for MTB. Effects of several metal salts, solvents, surfactants, and typical enzyme inhibitors on tannase activity were evaluated to establish the novelty of the enzyme. Finally, the tannase from A. niger GH1 was significantly inhibited by PMSF (phenylmethylsulfonyl fluoride), and therefore, it is possible to consider the presence of a serine or cysteine residue in the catalytic site.