• 한국양식학회지
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• 제21권4호
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• pp.252-258
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• 2008

전라남도 여수시 소라면 여자만에서 2007년 3월부터 2008년 2월까지 채집된 새꼬막 Scapharca subcrenata의 생식소 발달과 생식주기를 조직학적으로 조사하였다. 새꼬막은 자웅이체로 난소는 많은 난소 소낭(oogenic follicle)으로 이루어져 있으며, 정소도 많은 정소 소낭(acinus)으로 구성되어 있다. 생식소지수(GI)는 6월에 연중 최고 값을 나타내었고, 그 후 감소하여 7월에 최저 값를 나타내었다. 비만도지수(CI)는 4월에 연중 최고 값을 보였고, 그 후 감소하여 7월에 최저 값을 보였다. 새꼬막의 생식주기는 초기 활성기($1{\sim}4$월), 후기 활성기($3{\sim}6$월), 완숙기($5{\sim}8$월), 방출기($7{\sim}9$월) 그리고 회복 및 휴지기($9{\sim}3$월)의 연속적인 발달 단계로 구분되었으며, 주 산란기는 $7{\sim}8$월로 조사되었다. 암, 수 성비는 1:1로 유의한 차를 보이지 않았다.

• 한국패류학회지
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• 제23권2호
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• pp.165-172
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• 2007

우리나라 남해안 강진만에서 2006년 5월부터 2007년 4월까지 채집된 개조개의 생식소 발달과 생식주기를 조직학적으로 조사하였다. 개조개는 자웅이체로 난소는 수많은 난소소낭 (oogenic follicle) 으로 이루어져 있으며, 정소는 다수의 정소 소낭 (acinus) 으로 구성되어 있다. 생식소지수는 4월에 최고값을 나타내었고, 그 후 감소되어 7월에 최저값를 나타내었다. 비만도지수는 6월에 최고값을 보였고, 그 후 감소되어 11월에 최저값을 보였다. 개조개의 생식주기는 회복 및 휴지기 (6-12월), 초기활성기 (1-4월), 후기활성기 (2-7월), 완숙기 (3-10월), 방출기 (4-10월)로 구분되었다. 강진만에서 개조개의 주산란기는 7-9월이었다. 암, 수 성비는 1:1로 유의한 차를 보이지 않았다.

• 한국수정란이식학회지
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• 제17권3호
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• pp.203-209
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• 2002

본 실험은 원시난포의 체외배양 체계를 확립할 수 있는 가능성을 검토하기 위해 10일과 20일령 마우스의 난소에서 분리한 난포를 체외배양하여 난포의 형태변화, 난자의 성장를 및 난자의 핵성숙단계를 조사하여 비교하였다. 그 결과를 요약하면 다음과 같다. 1. 10일령과 20일령의 마우스에서 분리한 난포는 배양 6일째부터 난포강이 형성되었다. 2. 10일령과 20일령 마우스의 난소에서 분리한 난포의 수는 평균 21.5와 33.3개였고, 배양하기 전에 투명대를 제외한 난자의 직경은 51.85 $mu extrm{m}$와 57.50 $\mu\textrm{m}$였으나, 10 일간 배양한 후 측정한 난자의 직경은 55.95 $\mu\textrm{m}$와 63.11 $\mu\textrm{m}$로 증가하였다. 3. 10일령과 20일령에서 M II까지 핵이 성숙된 난자는 각각 4.3%와 22.1%였고. GVBD기 이상은 각각 14.5%와 61.1%였다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권9호
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• pp.1270-1275
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• 2014

An experiment was conducted to evaluate the efficacy of porcine follicle stimulating hormone (pFSH) dosage based on body weight (BW) on ovarian responses of crossbred does. Thirty donor does were divided into 3 groups getting pFSH dosages of 3, 5, and 8 mg pFSH per kg BW, respectively, and were named as pFSH-3, pFSH-5 and pFSH-8, respectively. Estrus was synchronized by inserting a controlled internal drug release (CIDR) device and a single injection of prostaglandin $F2{\alpha}$ ($PGF2{\alpha}$). The pFSH treatments were administered twice a day through 6 decreasing dosages (25, 25, 15, 15, 10, and 10% of total pFSH amount; decreasing daily). Ovarian responses were evaluated on Day 7 after CIDR removal. After CIDR removal, estrus was observed 3 times in a day and pFSH treatments were initiated at 2 days before the CIDR removal. All does in pFSH-5 and pFSH-8 showed estrus signs while half of the does in pFSH-3 showed estrus signs. No differences (p>0.05) were observed on the corpus luteum and total ovarian stimulation among the treatment groups, while total and transferable embryos were higher (p<0.05) in pFSH-5 (7.00 and 6.71) than pFSH-3 (3.00 and 2.80) and pFSH-8 (2.00 and 1.50), respectively. In conclusion, 5 mg pFSH per kg BW dosage gave a higher number of embryos than 3 and 8 mg pFSH per kg BW dosages. The results indicated that the dosage of pFSH based on BW is an important consideration for superovulation in goats.

• 한국수정란이식학회지
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• 제15권1호
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• pp.1-8
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• 2000

The ultrasound-guided oocytes cllection (ovum pick-up ; OPU) has become a substitution for superovlation in cattle. The objective of this study was to examine the effect of OPU frequency on the in vitro production of embryos in Hanwoo cattle. Six cycling Hanwoo cows were distributed into two groups for either once or twice weekly OPU sessions. Oocytes were collected by ultrasound-guided follicle aspiration(SA600) using a 6.5HMz transducer and attached with 18 gauge needle, with vacuum pressure of 40 mmHg. The cumulus-oocyte complexes (COCs) collected from each donor were matured in TCM 199 supplemented with 10% fetal bovine serum at 5% CO2 in air at 38.5$^{\circ}C$ for 22h and in vitro matured oocytes were co-incubated with sperm(separated by Percoll gradient) for 6h. The zygotes were co-cultured on cumulus cell monolayer in 10ul droplets in the same culture medium and conditions used for IVM for 7 days. On Day 7 of culture, development to blastocysts was examined. Although the number of oocytes collected was variable depending on individuals, overall embryo production in the twice per week OPU sessions was better that in the once per week sessions(6~21 vs 2~7 blastocysts produced, respectively). Two cows(E, A) were good oocyte donors and embryo production was superior in cow C ; however, cow F was a poor donor as compared to the others. In conclusion, these results suggest that for embryo production, twice weekly OPU sessions were better than once per week for producing embryos in vitro from Hanwoo cattle.

• 한국양식학회지
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• 제22권1호
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• pp.34-41
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• 2009

전라남도 여수시 소라면 여자만에서 2002년 1월부터 12월까지 채집된 꼬막의 생식소 발달과 생식주기를 조직학적으로 조사하였다. 꼬막은 자웅이체로 난소는 많은 난소 소낭(oogenic follicle)으로 이루어져 있으며, 정소도 많은 정소 소낭(acinus)으로 구성되어 있다. 생식소지수(GI)는 6월에 연중 최고 값을 나타내었고, 그 후 감소하여 8월에 최저 값을 나타내었다. 비만도지수(CI)는 7월에 연중 치고 값을 보였고, 그 후 감소하여 9월에 최저를 나타냈다. 꼬막의 생식주기는 초기 활성기($3{\sim}5$월), 후기 활성기($4{\sim}6$월), 완숙기($5{\sim}7$월), 방출기($7{\sim}8$월) 그리고 회복 및 휴지기($9{\sim}3$월)의 연속적인 발달 단계로 구분되었으며, 주 산란기는 $7{\sim}8$월로 조사되었다. 암 수 성비는 1:1로 유의한 차를 보이지 않았다.

• Clinical and Experimental Reproductive Medicine
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• 제48권1호
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• pp.69-79
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• 2021

Objective: Poor ovarian response (POR) refers to a subnormal follicular response that leads to a decrease in the quality and quantity of the eggs retrieved after ovarian stimulation during assisted reproductive treatment (ART). The present study investigated the associations of multiple variants of the estrogen receptor 2 (ESR2) and follicle-stimulating hormone receptor (FSHR) genes with POR in infertile Jordanian women undergoing ART. Methods: Four polymorphisms, namely ESR2 rs1256049, ESR2 rs4986938, FSHR rs6165, and FSHR rs6166, were investigated in 60 infertile Jordanian women undergoing ART (the case group) and 60 age-matched fertile women (the control group), with a mean age of 33.60±6.34 years. Single-nucleotide polymorphisms (SNPs) were detected by restriction fragment length polymorphism and then validated using Sanger sequencing. Results: The p-value of the difference between the case and control groups regarding FSHR rs6166 was very close to 0.05 (p=0.054). However, no significant differences were observed between the two groups in terms of the other three SNPs, namely ESR2 rs1256049, ESR2 rs4986938, and FSHR rs6165 (p=0.561, p=0.433, and p=0.696, respectively). Conclusion: The association between FSHR rs6166 and POR was not statistically meaningful in the present study, but the near-significant result of this experiment suggests that statistical significance might be found in a future study with a larger number of patients.

• Animal Bioscience
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• 제37권1호
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• pp.50-60
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• 2024

Objective: Testicular fat deposition has been reported to affect animal reproduction. However, the underlying mechanism remains poorly understood. The present study explored whether sperm meiosis and testosterone synthesis contribute to mouse testicular fat deposition-induced reproductive performance. Methods: High fat diet (HFD)-induced obesity CD1 mice (DIO) were used as a testicular fat deposition model. The serum hormone test was performed by agent kit. The quality of sperm was assessed using a Sperm Class Analyzer. Testicular tissue morphology was analyzed by histochemical methods. The expression of spermatocyte marker molecules was monitored by an immuno-fluorescence microscope during meiosis. Analysis of the synthesis of testosterone was performed by real-time polymerase chain reaction and reagent kit. Results: It was found that there was a significant increase in body weight among DIO mice, however, the food intake showed no difference compared to control mice fed a normal diet (CTR). The number of offspring in DIO mice decreased, but there was no significant difference from the CTR group. The levels of follicle-stimulating hormone were lower in DIO mice and their luteinizing hormone levels were similar. The results showed a remarkable decrease in sperm density and motility among DIO mice. We also found that fat accumulation affected the meiosis process, mainly reflected in the cross-exchange of homologous chromosomes. In addition, overweight increased fat deposition in the testis and reduced the expression of testosterone synthesis-related enzymes, thereby affecting the synthesis and secretion of testosterone by testicular Leydig cells. Conclusion: Fat accumulation in the testes causes testicular cell dysfunction, which affects testosterone hormone synthesis and ultimately affects sperm formation.

• Asian-Australasian Journal of Animal Sciences
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• 제11권1호
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• pp.65-70
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• 1998

To understand the caused for poor response to superovulation in water buffalo compared to crossbred cows, follicular events, before start of superovulation, during superovulation and after superovulation were compared. Follicular development was monitored a day before start of superovulation, daily upto superestrus and on the day of flushing. A real time B mode diagnostic instrument equipped with a linear array, 5 MHz transducer was used in five crossbred cows and five Murrah buffaloes. Crossbred cows yielded significantly (p < 0.01) higher number of corpora lutea than buffaloes (21 vs 10). The mean number of small size (2 to 5 mm); medium size (6 to 9 mm) and large size $({\geq}10mm)$ follicles, a day before start of superovulation were almost similar or even slightly higher in buffalo. Though initial shift in the mean number of follicles was higher in buffalo than cow, yet, from Day 2 to Day 3 of the treatment, the average increase in medium (3.2 vs 1.2) and large size (5.0 vs 2.0) follicles was higher in cows than buffaloes. The mean number of medium and large size follicles was 9.8 and 14.4 in cows and 6.4 and 7.6 in buffaloes. On the day of flushing, the number of large size follicle was more in buffaloes than cows, indicating the ovulation problem in this species. The major conclusion from this investigation was that, a day before start of superovulatory treatment, the number of small and medium size follicles was slightly higher in buffaloes, even then superovulatory response was better in cows, due to shift, recruitment and passage of follicles from smaller size to larger size from Day 2 of treatment. Ovulation problem in buffaloes was also responsible for lower superovulatory responses as revealed by the presence of higher number of large size follicles on the day of flushing.

• 식물분류학회지
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• 제48권3호
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• pp.230-247
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• 2018

쉬땅나무속 분류군의 형태적 변이를 파악하고, 각 분류군의 식별형질을 검토하기 위해 정량적 형질을 이용하여 주성분분석(principal component analysis)을 수행하였다. 쉬땅나무속 분류군은 화서의 직립여부, 자방 및 골돌과 표면의 털 유무, 수술의 개수, 악편의 형태 등의 정성적 형질로 크게 두 그룹으로 구분된다. 본 연구 결과, 소엽의 개수 및 크기, 화서의 너비, 악편, 화판, 골돌과, 종자의 크기 등의 정량적 형질 또한 이들 두 그룹으로 뚜렷하게 구분되었다. 그룹 내에서는 Sorbifolia group (S. grandiflora, S. sorbifolia complex)의 경우, 측소엽의 크기와 엽맥의 수, 소엽 하면 내 선점 및 성상모의 개수, 화판, 골돌과의 크기 등의 형질이 유용한 식별형질로 파악되었으며, Kirilowii group (S. arborea complex, S. kirilowii, S. tomentosa complex) 내에서는 잎의 형질 중 정소엽과 측소엽의 크기 및 하면의 선점 및 성상모의 빈도 등이 분류군을 인식하는 데 유용한 것으로 파악되었다. 본 속의 수리분류학적 연구 결과는 추후 종 하 분류군을 포함한 쉬땅나무속 전체의 분류학적 재검토 및 분류학적 한계 설정에 매우 유용할 것으로 판단된다.