• Archives of Pharmacal Research
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• 제21권6호
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• pp.692-697
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• 1998

GTP cyclohydrolase I catalyzing the first reaction in the biosynthesis of pterin moiety of folic acid in bacteria, was purified from Streptomyces tubercidicus by at least 203-fold with a yield of 32% to apparent homogeneity, using ammonium sulfate fractionation, DEAE-cellulose, Sepharose CL-6B, and hydroxylapatite column chromatography. The molecular weight of the native enzyme was estimated to be 230,000 daltons by gel permeation chromatography. The purified enzyme gave a single band on sodium dodesyl sulfate-polyacrylamide gel electrophoresis and its molecular weight was apparently 58,000 daltons. These results indicate that the enzyme consists of four subunits with the same molecular weight. The $K_m$ and $V_{max}$ values for GTP of the purified enzyme were determined to be 80${\mu}$M and 90nmol/min (mg protein), respectively. The optimum pH and temperature for the enzyme reaction were pH 7.5-8.5 and $40-42^{\circ}C$, respectively. Coenzyme or metal ion was not required for the enzyme activity. The enzyme activity was inhibited by most divalent cations, while it was slightly activated by potassium ion. In case of nucleotides, CTP, GMP, GDP, and UTP inhibited enzyme activity, among which GDP exhibited the strongest inhibitory effect.

• Journal of Nutrition and Health
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• 제38권2호
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• pp.96-103
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• 2005

It has been suggested that the elevated plasma homocysteine may lead to retinal dysfunction. We investigated the effects of plasma levels of homocysteine and folate on the retinal glial cells' injuries. Male Sprague-Dawley rats were raised either on a control diet or on an experimental diet containing 3.0 g/kg homocystine without folic acid for 10 weeks. Plasma homocysteine concentrations were measured by a HPLC-fluorescence detection method. Plasma folate and vitamin B/sub 12/ levels were analyzed by a radioimmunoassay. The response of Muller cells which are the principal glial cells of the retina was immunohistochemically examined using an antibody for vimentin, a cytoskeletal protein belonging to the family of intermediate filament. At 2 weeks, the homocystine diet induced a twofold increase in plasma homocysteine, and a concomitant increase in the expression of vimentin in the Muller cells' processes spanning from the inner to outer membranes of the retina indicating arterial degeneration. At 10 weeks, the homocystine diet induced a fourfold increase in plasma homocystine, but vimentin immunoreactivity in the retinas was similar in both groups. In conclusion, increased plasma homocysteine levels have influence on morphological and functional changes of Muller cells in the retina. (Korean J Nutrition 38(2): 96～103, 2005)

• Proceedings of the Korean Society of Food Science and Nutrition Conference
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• 한국식품영양과학회 2004년도 Annual Meeting and International Symposium
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• pp.203-208
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• 2004

The Family of Man was a 1955 photographic exhibition that depicted normal people in all walks of life from around the world. The viewer was visually confronted with the similarity of all people. The people of the world are now facing common health challenges as a result of changing lifestyles and increasing healthcare costs. Dietary supplements have the potential to be part of the solution. Dietary supplementation with antioxidant vitamins may be the most effective intervention for preventing the onset of Alzheimer's disease in the rapidly expanding elderly population - and at a very low cost. AIDS is devastating much of the developing world, where few resources are available to treat it victims. Inexpensive multivitamin sup-plements have been demonstrated to slow the progression of AIDS, reduce the death rate, reduce AIDS-related morbidity, improve T cell counts, and reduce viral loads. Careful economic analysis demonstrate that calcium and folic acid supplementation could reduce healthcare costs in the USA by $13.9 billion and $1.3 billion, respectively. Most supplements with therapeutic potential have not been subjected to sufficiently rigorous research to permit unqualified public health recommendations. Carefully directed research is needed to identify the supplements with the greatest potential and to verify their safety and efficacy.

• Research in Community and Public Health Nursing
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• 제15권3호
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• pp.483-495
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• 2004

Purpose: The purpose of this study was to explore the perimenstrual discomforts and dietary intake levels among normal women. Method: A prospective and descriptive study examined 38 women aged 23 46years of age. The data collection period lasted from April 1 to June 30, 2003. The participants were asked to keep a diary recording perimenstrual symptoms and food intake for 50 days. Results: There was a significant difference in physical symptoms of perimenstrual discomforts (F=6.95. p=.001). but there was no significant difference in dietary intake level according to three different phases of a menstrual cycle. The significant dietary intake variables correlated to PMS included energy, protein, Vitamin E, Vitamin $B_2$, niacin, Vitamin $B_6$, folic acid, phosphorus, iron, and zinc. They were negatively related to perimenstrual discomforts. Conclusion: Balanced diet intake will be necessary for not only the perimenstrual discomforts but also the general health promotion of the entire population. The dietary and nutritional assessment should be done prior to nursing interventions, and nutritional counseling and education should be given based on individual differences. In a further study, the effects of dietary composition on specific symptoms will be replicated with a large sample, and development of a diet intervention program for perimenstrual discomforts is recommended.

• The Journal of Internal Korean Medicine
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• 제21권5호
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• pp.877-881
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• 2000

Anemia is present in adult if the hematocrit is less than 41%(hemoglobin <13.5g/dL) in males or 37%(hemoglobin <12g/dL) in females. In taking the history, congenital anemia may be suggested by the patient's personal and family history. Poor diet results in folic acid deficiency and may contribute to iron deficiency. Bleeding should always be considered in iron deficiency. Object : The symptoms of this patient are easy fatigability, dizziness, anorexia and paleness of the face. This women is the patient of iron deficiency anemia, cerebral hemorrhage and hypertension. Medication : Gwibitang(歸脾湯) plus(合) Samultang(四物湯) and feroba are dispensed for 30 days. Result : After 30 days, HCT Hb Iron of the laboratory findings are increased and TIBC of the laboratory findings is decreased.

• Nutrition Research and Practice
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• 제5권3호
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• pp.230-235
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• 2011

Insomnia has become a major public health issue in recent times. Although quality of sleep is affected by environmental, psychophysiological, and pharmacological factors, diet and nutrient intake also contribute to sleep problems. This study investigated the association between nutrient intake and co-morbid symptoms associated with sleep status among selected adults. Subjects in this study included 87 men and women aged 21-45 years. Presence of insomnia was assessed using the Insomnia Screening Questionnaire, and dietary intake was measured over three consecutive days by dietary survey. Descriptive analysis, ANOVA, and Chi-Square tests were performed to compute and interpret the data. Approximately 60% of the participants were insomniacs. People with insomnia consumed significantly lesser quantities of nutrients as compared to normal sleepers. Differences in intakes of energy, carbohydrates, folic acid, and $B_{12}$ were highly significant (P < 0.002). Further, intakes of protein, fat, and thiamine were significantly different (P < 0.021) between insomniacs and normal sleepers. The nutrient intake pattern of the insomniacs with co-morbid symptoms was quite different from that of the normal sleepers. Based on these results, it is probable that there is an association between nutrition deficiency, co-morbid symptoms, and sleep status. More studies are required to confirm these results.

• Women's Health Nursing
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• 제10권2호
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• pp.162-170
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• 2004

Purpose: The purpose of this study was to differentiate between women with three perimenstrual symptom severity patterns : premenstrual syndrome(PMS), premenstrual magnification(PMM), and low symptom(LS), and to explore the related dietary factors to premenstrual symptoms. Method: Women were asked to keep a diary record of perimenstrual symptoms and food intake for 50 days. Result: Symptom patterns were defined for 26 among 38 women ; Eight(21.1%) demonstrated a PMS pattern, three(7.9%) demonstrated a PMM pattern, and fifteen(39.5%) exhibited a LS pattern. There were significant differences in symptom scores during the premenstrual phase($x^2=19.30$, p=.000), menstrual phase($x^2=13.32$, p=.001), and post menstrual phase($x^2=9.93$, p=.007) for three groups. Protein, vit E, vit C, niacin, folic acid, and phosphorus in the premenstrual phase, and energy, and vit B6 in the menstrual period were significantly different between the three groups. Among dietary compositions, amino acids, lipids, fatty acids, saturated fatty acids, natrium, vit B6, niacin, and vit E were negatively related to PMS symptoms. Conclusion: Pattern of perimenstrual symptoms should be differentiated for individualized PMS management. As a more efficient diet assessment for PMS women, randomized nutritional analysis during the 3 phases of the menstrual cycle should be done and a replication study is necessary with a larger sample.

• CELLMED
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• 제3권4호
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• pp.29.1-29.11
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• 2013

Certain dietary contents, biological supplements might influence the occurrence or treatment of epilepsy. Some studies have found that the supplementation with individual nutrients reduced seizure frequency or improved other aspects of health in patients with epilepsy. Potentially beneficial dietary interventions include treating blood glucose dysregulations. Identifying and avoiding allergenic foods, and avoiding suspected triggering agents such as alcohol, aspartame, and monosodium glutamate. The Atkins diet (very low in carbohydrates) is a less restrictive type diet that may be effective in some cases. Nutrients that may reduce seizure frequency include vitamin B6, magnesium, vitamin E, manganese, taurine, dimethylglycine, and omega-3 fatty acids. Administration of thiamine may improve cognitive function in patients with epilepsy. Supplementation with folic acid, vitamin B6, biotin, vitamin D, and L-carnitine may be needed to prevent or treat deficiencies resulting from the use of anticonvulsant drugs. Vitamin K1 has been recommended near the end of pregnancy for women taking anticonvulsants. Melatonin may reduce seizure frequency in some cases, and progesterone may be useful for women with cyclic exacerbations of seizures. In most cases, nutritional therapy is not a substitute for anticonvulsant medications. However, in selected cases, depending on the effectiveness of the interventions, dosage reductions or discontinuation of medications may be possible. However, nutrient supplementation may be necessary to prevent or reverse the effects of certain deficiencies that frequently result from the use of antiepileptic drugs.

• Journal of Nutrition and Health
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• 제40권8호
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• pp.728-735
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• 2007

The present study was conducted to investigate the effect of iron-enriched cereal supplement and nutrition education program on nutritional status and life style of children. Elementary school students(n=170) aged of 11 years old at Seoul were participated. Subjects were supplemented with iron-enriched cereal for breakfast and provided with 2 sessions of nutrition education during 3 months. Intake of carbohydrate, iron, sodium, zinc, vitamin A, vitamin $B_1$, vitamin $B_2$, vitamin C, niacin, and folic acid was significantly increased after cereal supplement. Caloric intake was similar to KDRIs during both before and after cereal supplement. There was no improvement on exercise and dietary habits after nutrition education. Male students tended to sleep later, wake up early, and ate snack less than female. Cereal supplement was effective on improving nutritional status, but short-term nutrition education had no effect on modification of life style. Thus, further studies are required to develop long-term education program effective on improving lift style and eating habits in children.

• Nutrition Research and Practice
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• 제5권1호
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• pp.3-10
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• 2011

Women's nutrition has received little attention in nutrition programming, even though clinical trials and intervention trials have suggested that dietary improvement or supplementation with several nutrients may improve their health, especially in low-income settings, the main focus of this paper. Most attention so far has focused on how improvements in maternal nutrition can improve health outcomes for infants and young children. Adequate vitamin D and calcium nutrition throughout life may reduce the risk of osteoporosis, and calcium supplementation during pregnancy may reduce preeclampsia and low birth weight. To reduce neural tube defects, additional folic acid and possibly vitamin $B_{12}$ need to be provided to non-deficient women before they know they are pregnant. This is best achieved by fortifying a staple food. It is unclear whether maternal vitamin A supplementation will lead to improved health outcomes for mother or child. Iron, iodine and zinc supplementation are widely needed for deficient women. Multimicronutrient supplementation (MMS) in place of the more common iron-folate supplements given in pregnancy in low-income countries may slightly increase birth weight, but its impact on neonatal mortality and other outcomes is unclear. More sustainable alternative approaches deserve greater research attention.