• Journal of Plant Biotechnology
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• 제42권3호
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• pp.265-270
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• 2015

더덕은 예로부터 약용으로 사용 되어 왔으며, 더덕의 뿌리에는 약용으로 가치가 높은 여러 종류의 triterpenoid 사포닌이 포함되어 있다. 본 연구에서는 더덕의 모상근 생육과 methyl jasmonate (MeJA)처리에 의한 사포닌 합성의 효과를 연구하였다. 더덕 모상근에 MeJA를 처리한 결과 주사포닌인 lancemaside A, B, E의 축적은 MeJA 무처리 모상근 보다 약 15% 정도 감소하였다. 반면 마이너사포닌(foetidissimoside A와 aster saponin Hb)의 함량은 무처리 모상근 보다 약15% 정도로 증가하였다. 이 결과를 통해 MeJA처리가 triterpene 사포닌의 생산조절을 위해 사용될 수 있는 것으로 판단된다.

• 한국약용작물학회지
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• 제22권1호
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• pp.1-7
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• 2014

Codonopsis lanceolata (Campanulaceae) has been used in traditional medicines, as its roots contain several kinds of 3,28-bidesmosidic triterpenoid saponin with high medicinal values. In this study, we induced hairy root-derived transgenic plants of C. lanceolata and analyzed triterpenoid saponins from the leaf, stem and root. Transgenic plants were regenerated from the hairy roots via somatic embryogenesis. The saponins are lancemaside A, B and E, foetidissimoside A, and aster saponin Hb. Transgenic plants contained richer triterpenoids saponin than wild-type plants. Major saponin lancemaside A was the most abundant saponin in the stem from transgenic-plant, $4.76mg{\cdot}1^{-1}dry$ stem. These results suggest that transgenic plants of C. lanceolata could be used as medicinal materials for the production of triterpene saponins.

• Plant Biotechnology Reports
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• 제5권3호
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• pp.255-263
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• 2011

Codonopsis lanceolata (Campanulaceae) has been used in traditional medicines, as its roots contain several kinds of triterpenoid saponin with high medicinal values. In this work, we induced transgenic hairy roots of C. lanceolata and analyzed triterpenoid saponins from the hairy roots and hairy root-derived transgenic plants. Hairy roots were obtained from leaf explants by the transformation of Agrobacterium rhizogenes R1000. Transgenic hairy root lines were confirmed by the transcriptional activities of rolA, B, C, and D genes by RT-PCR. Transgenic root lines actively proliferated on hormone-free medium but not in nontransformed roots. Hairy roots contained richer triterpenoids (lancemaside A, foetidissimoside A, and aster saponin Hb) than nontransformed roots. Transgenic plants were regenerated from the hairy roots via somatic embryogenesis. They showed phenotypic alterations such as shortened shoots and an increased number of axillary buds and adventitious roots. The transgenic plants also contained higher triterpenoid levels than wild-type plants. These results suggest that hairy roots and transgenic plants of C. lanceolata could be used as medicinal materials for the production of triterpene saponins.