• Nuclear Engineering and Technology
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• 제25권2호
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• pp.255-258
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• 1993

펄스형 질소레이저를 사용한 time-resolved laser-induced fluorimetry를 이용하여 우라늄 작업자의 뇨속에 함유되어 있는 우라늄의 농도를 간단한 전처리만으로 정량분석하는 방법을 연구하였다. 형광분석할 때에 뇨에 함유된 chloride ion은 우라늄 형광을 심하게 quenching하는 것으로 알려져 있으며, 따라서 이를 제거하기 위한 전처리 과정에서 많은 시간 소모와 큰 실험 오차를 유발하고 있다. 본 방법에서는 10% Fluran 수용액을 뇨에 첨가하여 뇨함량이 약 1% 정도가 되었을 때 뇨속의 chloride에 의한 quenching 영향을 최소한으로 줄일 수 있었으며, 시간 0에서의 형광강도를 계산하여 형광강도의 농도에 대한 직선성을 측정한 결과 우라늄 농도 범위 10-500 ppb에서 우수한 직선성을 나타내었다.

• 분석과학
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• 제10권1호
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• pp.43-52
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• 1997

생물학적 분비물에서 히스타민의 측정시 HPLC-fluorimetry가 많이 응용되어 발달되어 왔다. 그러나 시간 소비와 HPLC system을 최적의 운영조건으로 유지시키는 것이 상대적으로 어렵기 때문에 직접 형광법을 이용하여 히스타민을 분석하였다. 표준 용액에서 형광측정법의 경우 검출 한계는 $3.0{\times}10^{-2}{\mu}g/mL$였고 이 때 상대표준편차는 3.74%~16.2%였다. 시료에서 HPLC-fluorimetry와 형광측정법 사이의 상대적인 히스타민 농도비율은 1.5~3.5배의 차이를 보였다. 표준물첨가법에 의한 각각의 시료에 대한 수득률은 100%~125%였다.

• Nuclear Engineering and Technology
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• 제25권4호
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• pp.548-551
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• 1993

원자력 산업의 변환공정에서 발생하는 폐수용액중의 우라늄 농도를 레이저 유발형광법을 사용하여 쉽게 측정할 수 있는 새로운 분석방법을 개발하였다. 실험 결과 시료의 약 10배 이상의 부피로 4M-인산용액을 첨가함으로써 효율적으로 형광 quencher의 영향을 배제시킬 수 있었으며, 폐액 시료에서의 우라늄 농도 3.0$\times$$10^{-6}$－6.0$\times$$10^{-5}$ M U $O_2$$^{2＋}$의 범위에서 농도에 대한 형광강도의 직선성이 우수하였다.

• Bulletin of the Korean Chemical Society
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• 제24권1호
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• pp.45-48
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• 2003

A novel fluorimetric method has been developed for the determination of microgram quantities of bovine serum albumin (BSA) based on its enhancement effect of Nile Blue fluorescence at 670 nm, caused by binding of Nile Blue to BSA to produce a stable water soluble complex. The binding constant of micromole Nile Blue-BSA complex was estimated by Scatchard plot method. Under the optimal conditions, the increased fluorescence intensity was linearly related to BSA concentration in the range of 0.5-12.0 ㎍/mL. The detection limit was 0.2 ㎍/mL, and the relative standard deviation of six replicate measurements was 1.4% for 10.0 ㎍/mL BSA. There was little interference from amino acids, sugars and most of metal ions.

• Bulletin of the Korean Chemical Society
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• 제10권5호
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• pp.411-414
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• 1989

A new sensitive fluorimetric method for the determination of N-methylcarbamates, a class of well known insecticides, based on the derivatization with 7-chloro-4-nitrosobenz-2-oxa-1,3-diazole (NBD-Cl) has been developed. Unreacted NBD-Cl was eluted ahead of derivatized carbamates from C-18 bonded column. An argon ion laser was used as an excitation source of chromatographic eluents and its fluorescence signal was monitored with optical multichannel analyzer. The detection limits of various carbamates were about 100 pg range and the working curves were linear to $10^4-10^5$ nanogram ranges.

• Journal of mucopolysaccharidosis and rare diseases
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• 제3권1호
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• pp.14-19
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• 2017

Lysosomal storage diseases (LSDs) are a group of rare inherited metabolic disorders caused by the deficiency of specific lysosomal enzymes and subsequent accumulation of substrates. Enzyme deficiency leads to progressive intra-lysosomal accumulation of the incompletely degraded substances, which cause dysfunction and destruction of the cell and eventually multiple organ damage. Patients have a broad spectrum of clinical phenotypes which are generally not specific for some LSDs, leading to missed or delayed diagnosis. Due to the availability of treatment including enzyme replacement therapy (ERT) and hematopoietic stem cell transplantation for some LSDs, early diagnosis is important. ERT products have been approved with optimal outcomes for some LSDs in the recent decades, including Gaucher, Fabry, mucopolysaccharidosis (MPS) I, Pompe, MPS VI, MPS II, and MPS IVA diseases. ERT can stabilize the clinical condition, prevent disease progression, and improve the long-term outcome of these diseases, especially if started prior to irreversible organ damage. Based on the availability of therapy and suitable screening methods in the recent years, some LSDs, including Pompe, Fabry, Gaucher, MPS I, MPS II, and MPS VI diseases have been incorporated into nationwide newborn screening panels in Taiwan.

• Journal of Microbiology and Biotechnology
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• 제28권11호
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• pp.1782-1790
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• 2018

Assessment of microorganism viability is useful in many industrial fields. A large number of methods associated with the use of fluorescent probes have been developed, including fluorimetry, fluorescence microscopy, and cytometry. In this study, a microvolume spectrofluorometer was used to measure the membrane potential variations of Escherichia coli. In order to estimate the sensitivity of the device, the membrane potential of E. coli was artificially disrupted using an ionophore agent: carbonyl cyanide 3-chlorophenylhydrazone. The membrane potential was evaluated using two ratiometric methods: a Rhodamine 123/4',6-diamidino-2-phenylindole combination and a JC-10 ratiometric probe. These methods were used to study the impact of freezing on E. coli, and were compared with the conventional enumeration method. The results showed that it was beneficial to use this compact, easy-to-use, and inexpensive spectrofluorometer to assess the viability of bacterial cells via their membrane potential.

• 대한화학회지
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• 제26권4호
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• pp.218-223
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• 1982

chl-a는 $5.12{\times}10-6M$ 이상의 농도와 건조된 ethyl ether, benzene, iso-octane중에 소중합체로 존재하고 이 용매에 n-prOH를 소량씩 가할때 단위체로 변함을 확인하였다. 형광은 중합체인 경우에 세게 나타나지만 중합정도에 따라 변하며 단위체인 경우는 약하게 나타났다. 그리고 n-prOH를 용매에 가할 때 흡광과 형광의 ${\lambda}_{max}$은 모두 장파장쪽으로 이동하였다. soret/red band의 비는 흡광도가 감소할수록 작아졌으며 chl-a의 농도에 따른 흡광도는 $1.0{\times}10^{-6}$M 정도의 용액에서 최대값을 나타냈다.

• 한국작물학회지
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• 제46권5호
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• pp.375-379
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• 2001

Transgenic plants from hypocotyl segments of buckwheat were produced with the Agrobacterium strain LBA4404 harboring the binary vector pBI121 containing chimeric genes of neomycin phosphotransferase II (npt II) and $\beta$-glucuronidase (gus). Two weeks after co-cultivation with Agrobacterium, most of the hypocotyl segments gradually became brown and died on the selection medium containing 100mg/$\ell$ of kanamycin. Plants regenerated from the hypocotyl explants grown on selection medium were GUS-positive in the leaf, stem and vascular tissues by histochemical assay, and varied in gus activity (440-2568 pmol, 4-MU/mg protein) by fluorimetry. The plants showing GUS activity were confirmed of containing GUS and NPT-II genes by polymerase chain reaction (PCR). Within 3 months, transgenic buckwheat plants were able to obtained from the hypocotyl segments.

• ALGAE
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• 제29권4호
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• pp.321-331
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• 2014

Vertebrata lanosa is an abundant and obligate red algal epiphyte of Ascophyllum nodosum that forms part of a complex and highly integrated symbiotic system that includes the ascomycete, Mycophycias ascophylli. As part of ongoing studies to resolve interactions among species in the symbiosis, we used pulse amplitude modulation fluorimetry of chlorophyll a fluorescence, from photosystem II (PSII), to measure the maximum quantum yield ($F_v/F_m$) of PSII [$QY(II)_{max}$] and relative photosynthetic electron transport rates (rETR), as a function of light intensity, in order to evaluate the photosynthetic capacity of the two algal symbionts in the field and in the laboratory under different treatments. Our primary question was 'Is the ecological integration of these species reflected in a corresponding physiological integration involving photosynthetic process?' In the laboratory we measured changes in $QY(II)_{max}$ in thalli of V. lanosa and A. nodosum over one week periods when maintained together in either attached or detached treatments or when maintained separated from each other. While the $QY(II)_{max}$ of PSII of A. nodosum remained high and showed no significant variation among treatments, V. lanosa showed decreasing performance in the following conditions: V. lanosa attached to A. nodosum, V. lanosa in the same culture, but not attached to A. nodosum, and V. lanosa alone. These results are consistent with observations in which rETR was reduced in V. lanosa maintained alone versus attached to A. nodosum. Values for $QY(II)_{max}$ in V. lanosa measured in the field in fully submerged thalli were similar to those measured in the laboratory when V. lanosa was attached to it obligate host A. nodosum. Our results provide evidence of a physiological association of the epiphyte and its host that reflects the known ecology.