• Title/Summary/Keyword: Fluorescent substances

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Photo-induced Electron Transfer(PET) Based Luminescent Chemosensors Detecting Hazardous Substances (광유발 전자전이를 이용한 유해물질 인식 형광 화학센서의 개발)

  • Yoon, Sung-Ho
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.25 no.9
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    • pp.711-716
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    • 2012
  • Based on both organic synthesis and theoretical calculations on the effects of molecular orbital energy levels of amines on the fluorescence properties of the fluorophore, fluorescent "turn-on" chemosensors detecting hazardous substances, including aldehyde chemicals and $Hg^{2+}$ ion, were developed.

Changes of Fluorescent Substances and Free Amino Acids in Formed Eggs of the Silkworm(Bombyx mori) (가잠조성난의 형광물질과 유리아미노산의 변화)

  • 성수일;박광의;김주읍
    • Journal of Sericultural and Entomological Science
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    • v.20 no.1
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    • pp.11-14
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    • 1978
  • This experiment was attempted to investigate the effect of removal of suboesophageal ganglion of Bombyx mori on the changes of free amino acids and fluorescent substances by means of paper chromatography. 1. There are no differences of quantity and component of free amino acids of formed eggs in moths between treatment and control. 2. Riboflavine content of formed eggs in treated moths is less than that of formed eggs in untreated moths. 3. Fluorescent substance which appeared on Rf value 0.06 was not detected in formed eggs of treated moths but found in those of control. 4. Fluorescent substance which appeared on Rf value 0 was noted in formed eggs of treated moths but not in those of control.

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Sorting of Aged Seeds Using Leakage Substances of Crop Seeds (종자 누출물질을 이용한 노화종자의 선별)

  • Lee, Dong-Jin;Lim, Moo-Sang;Chu, Young-Ha;Chung, Kil-Woong;Kim, Bong-Ku
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.6
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    • pp.783-789
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    • 1997
  • This study was conducted to identify leakage of fluorescent substances out of 56 crops and to develop sorting methods of nonviable seeds of Chinese cabbage. Fluorescent substances were detected from 36 species, Chinese cabbage, radish, broccoli, cauliflower, egg plant and rape seed etc. In Chinese cabbage, the germination rate of non-sorted seeds decreased rapidly with ageing of seeds at 45$^{\circ}C$ and 100%. After Chinese cabbage seeds soaked for 4hrs and coated with cellulose, the sorting ratio of non fluorescent seeds were decreased 91.3% to 1.7% by increasing ageing periods from control to 8 days, while fluorescent seeds were increased drastically from 8.7% to 98.3%. Also, incubation of water soaked dead seeds of Chinese cabbage treated with 0.5-1.0% ninhydrin and 0.01N sodium thiosulfate for 30~60 minutes at 35$^{\circ}C$ developed purple color.

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Scanning Backlight Driver for Mercury Free Flat Fluorescent Lamp (무수은 면광원 램프용 Scanning Backlight 구동회로)

  • Oh, Eun-Tae;Jung, Yong-Min;Lee, Kyung-In;Yoo, Ho-Won;Lee, Jun-Young
    • The Transactions of the Korean Institute of Power Electronics
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    • v.14 no.1
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    • pp.8-14
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    • 2009
  • A lamp which is currently employed to LCD(Liquid Crystal Display) Backlight is almost CCFL(Cold Cathod Fluorescent Lamp) and EEFL(External Electrode Fluorescent Lamp). However, the use of these lamps is being restricted as RoHS(the Restriction of the use of certain Hazardous Substances in electrical and electronic equipment) regulation is gradually reinforced. According to this situation, the manufacturing of a lamp which doesn't use mercury is unescapable. Moreover, LCD TV has a defect which take place Motion Blur phenomenon due to response time of LC(Liquid Crystal), and Hold-type characteristic which only exists in LCD differently to CRT, PDP. In this paper, an inverter is proposed to drive a plane light source lamp which is not containing mercury. Driving circuit of proposed inverter is simple because the number of semiconductor device and magnetic device is reduced by using forward topology. Also, Motion Blur phenomenon is decreased by dividing the plane light source lamp to six block along vertical direction, and scanning. Finally, we proved usefulness of proposed inverter through experiment.

Suppressive Effects of Coumarins on Pumpkin Seedling Growth and Glutathione S-Transferase Activity

  • Hossain, Md. Daud;Li, Jing;Guo, Shirong;Fujita, Masayuki
    • Journal of Crop Science and Biotechnology
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    • v.11 no.3
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    • pp.187-192
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    • 2008
  • The effects of some coumarins(coumarin, 7-hydroxycoumarin, scopoletin and esculetin) were investigated on pumpkin(Cucurbita maxima Duch.) seedlings and on pumpkin glutathione S-transferases(GSTs). Coumarin and esculetin suppressed the growth of seedlings, especially the elongation of roots as well as hypocotyls. Among the compounds tested, only esculetin inhibited the activity of a particular pumpkin GST by 50%, CmGSTU3 toward 1-chloro-2, 4- dinitrobenzene(CDNB) and at a concentration of 22 ${\mu}M$. Both ethylacetae(EtOAc) and water fractions in pumpkin seedlings and different organs of one-month-old pumpkin plants contained esculetin or similar hydrophobic fluorescent substances as well as hydrophilic substances, which showed different degrees of inhibitory effects on CmGSTU3 activity.

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Domestic Research Trends on Fluorescent Dissolved Organic Matter in Marine Environment (해양 환경의 형광용존유기물에 관한 국내 연구 동향)

  • Kim, Jeonghyun
    • Ocean and Polar Research
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    • v.43 no.4
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    • pp.353-363
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    • 2021
  • Fluorescent dissolved organic matter (FDOM) is referred to organic matter which absorbs efficiently solar radiation energy and fluorescence in the water column. The component and molecular structure of marine organic matter can be changed depending on the various substances and origins of organic matter, and then the organic matter has unique fluorescent properties. As the cutting-edge analytical techniques of optical measurement continuously developing from last few decades, a study on FDOM has been applied as a biogeochemical tracer to quantify the organic matter concentration and to investigate the behaviors and origins of organic matter. Especially, the marine environment around the Korean Peninsula is an ideal research area to study FDOM because of various oceanographic characteristics and the origins of organic matter. This study describes the general properties of FDOM and introduces the cycling and behaviors of marine organic matter based on the domestic research studies.

Effect of Light-Emitting Diodes on Cordycepin Production in Submerged Culture of Paecilomyces japonica

  • HA, Si Young;JUNG, Ji Young;YANG, Jae-Kyung
    • Journal of the Korean Wood Science and Technology
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    • v.48 no.4
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    • pp.548-561
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    • 2020
  • Paecilomyces japonica is widely cultured to produce mycelium for medicinal and health food use. Illumination is an important factor in the growth and production of mycelium in submerged culture. The effects of different light-emitting diode (LED) combinations on the growth and cordycepin content as bioactive substances of mycelium were investigated. The results showed that the mycelium dry weights were lower under dark condition and red LED treatments. Dark condition, fluorescent light, and ultraviolet-A failed to increase the cordycepin content. Blue light was necessary to increase the cordycepin content, and a red-to-blue ratio of 3:7 induced the highest cordycepin content. The cordycepin contents of mycelium in submerged culture were significantly higher in a 12 h/day illumination time under red and blue (red-to-blue ratio of 3:7) LED treatments, showing an increase of up to 38% compared with those under the fluorescent-light control condition. The results demonstrated the roles of light with different wavelengths on the biosynthesis of cordycepin as bioactive substances. The low-heat release and replacement of traditional fluorescent lights with low-energy-consuming LEDs could increase the contents of bioactive substances. After optimization of the cordycepin production using response surface methodology (Box-Behnken design) to its canonical form, the optimum combination was found to be as follows: illumination time = 17.7 h/day, sugar content in the medium = 9.7 g/50 mL, and incubation time = 61.2 h. The model predicted a maximum response of 3779.2 ㎍/mL cordycepin yield.

Development of Microfluidic Polydiacetylene Sensor Chip for pH detection (pH 검출을 위한 미세유동 폴리디아세틸렌 센서칩 개발)

  • Hwang, Hyun-Jin;Song, Si-Mon
    • Proceedings of the KSME Conference
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    • 2008.11b
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    • pp.2415-2418
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    • 2008
  • Polydiacetylenes (PDAs) are very attractive chemical substances which have distinctive features of color change and fluorescence emission by thermal or chemical stress. Especially, when PDAs contact with solutions of a particular pH, such as a strong alkaline sodium hydroxide (NaOH) solution or a strong acidic hydrogen chloride (HCl) solution, PDAs change their color from non-fluorescent blue to fluorescent red. In this study, we propose a novel method to detect alkaline pH using PDAs and NaOH solutions by hydrodynamic focusing on a microfluidic chip. Preliminary results indicate that the fluorescent intensity of PDAs increases in respond to the NaOH solution concentrations. Also, the fluorescence is quenched back when the PDAs are in contact with a HCl solution. These results are useful in a microfluidic PDA sensor chip design for pH detection.

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The Study on the Detection of Aflatoxins in the Fermentation Products and Cereals (TLC법에 의한 장류 및 공류중의 Aflatoxin검출에 관한 연구)

  • 한양일;김광호;오영복
    • Journal of Environmental Health Sciences
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    • v.5 no.1
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    • pp.46-50
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    • 1978
  • Aflatoxin, a mixture of the at least four toxic and carcinogenic metabolites, is known to be produced by only a few fungi. The toxins were designated aflatoxins because they were produced by the mold Aspergillus flavus(A. flavus). However, at least four other toxins and other species of the genus A. niger, A. parasiticus A. ruber and wentii have been reported to produce aflatoxins. And also the identical compounds may also be produced by molds, the Pencillium. At least four different species of Penicilliurn have been reported to produce aflatoxins (P. citrinurn, P. frequentans, P. puberulurn. and P. variable). So it is now known that the problem of Aflatoxin is not restricted to the single species A. flavus, even though that is a very common mold. Also additional aflatoxins have been discorvered. For sereral years, only four aflatoxins were known: $B_1, B_2, G_1$ and $G_2$, so designated by reason of their fluorescence and chromatographic charateristics. It is now known that there are really two new toxic materials in the milk. During the past year(1966) they were christened aflatoxin $M_1$ and $M_2$, since they were first found in milk. The two other and most recently discorvered aflatoxins were isolated late in 1966 from cultures of A. flavus, and were designated aflatoxin $B_2a$ and aflatoxin $G_2a$. In order to obtain a breaf information about extent of contamination of foodstuffs by aflatoxin which is known to produce eight different mold, aflatoxin detection of cereals and fermented foods on sale, such as polished rice, barley, wheat, wheat flour, lentil, red bean, soy bean, noodle, kochuj ang and Dwenjang (fermented soy bean paste) and chong Kuk, were carried out. The results of this investigation were summarized as follows: The hexane:$CHCl_3$ extracts of polished rice, barley wheat, wheat flour, lentil, red bean, noodle and kochujang yielded fluorescent spots on thin layer plates. However their Rfvalues were different from those of authentic aflatoxins. The fluorescent substances of the extract from soy bean, Dwenjang and chong kuk showed very similar Rf values to those of the standard aflatoxins. By two dimensional thin layer chromatography and comparison of ultra violet absorption spectra, it was found that these fluorescent substances were not aflatoxins. To conclude, aflatoxins themselves were not detected directly in those samples tested.

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Biochemical Study on Soft-rotted Sweetpotatoes (연부(軟腐)고구마의 생화학적(生化學的) 연구(硏究))

  • Chun, Jae-Kun
    • Applied Biological Chemistry
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    • v.10
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    • pp.47-61
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    • 1968
  • Sweetpotato infected by Soft-rot, Rhizopus nigricans, has been investigated for its biochemical changes. The results of the present experiment are summarized as follows: 1. From the Soft-rotted tuber, nineteen Ehrlich's positive substances and three fluorescent compounds which had not been found in a healthy tuber were detected with thin layer chromatography. 2, Ipomeamarone and umbelliferone, each of which are predominating substances among the Ehrlich's positive and fluorescent compounds, were isolated by silica gel column chromatography and studied for their identities. 3. Time course biosynthesis of the metabolites was studied and discussed for their possible biosynthetic pathways. 4. Among the metabolites, ipomeamarone and ipomeamarone-like substances have enzyme: inhibitive action against ${\alpha}$-amylase and probably other enzymes; the inhibition type was determined as the uncompetitive one. 5. Ipomeamarone obtained from soft-rotted sweetpotato was proved to have an uncoupling action as 2, 4-dinitrophenol.

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