• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 추계학술대회논문집B
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• pp.400-404
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• 2001

A micro-PIV(particle image velocimetry) measurement has been conducted to investigate flow fields in such microfluidic devices as microchannels and micronozzle. The present study employs a state-of-art micro-PIV system which consists of epi-fluorescence microscope, 620nm diameter fluorescent seed particles and an 8-bit megapixel CCD camera. Velocity vector fields with a resolution of $6.7\times6.7{\mu}m$ has been obtained, and the attention has been paid on the effect of varying measurement conditions of particle diameter and particle concentration on the resulting PIV results. In this study, the microfluidic elements were fabricated on plastic chips by means of MEMS processes and a subsequent molding process. Flow fields in a variety of microchannels as well as micronozzle have been investigated.

• 한국지구물리탐사학회:학술대회논문집
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• 한국지구물리탐사학회 2003년도 Proceedings of the international symposium on the fusion technology
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• pp.569-572
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• 2003

Titanium dioxide ($TiO_2$) nanoparticles were prepared by the oxidation of titanium tetrachloride ($TiCl_4$) in a diffusion flame reactor. The average diameter of particles was 15 to 30 nm and mass fraction of anatase ranged from $40\;to\;80\%$. Effects of particle size and phase composition of those $TiO_2$ nanoparticles on photocatalytic properties such as decomposition of methylene blue and bacteria gas were investigated. The degree of decomposition of methylene blue by the $TiO_2$ nanoparticles under the illumination of the black light was directly proportional to the anantase mass fraction, but inversely to the particle size. The decomposition of bacteria by the $TiO_2$ nanoparticles under the illumination of the fluorescent light showed the same trend as in the case of the methylene blue.

• 한국가시화정보학회:학술대회논문집
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• 한국가시화정보학회 2001년도 Proceedings of 2001 Korea-Japan Joint Seminar on Particle Image Velocimetry
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• pp.44-52
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• 2001

A micro-PIV(particle image velocimetry) measurement has been conducted to investigate flow fields in such microfluidic devices as microchannels and micronozzle. The present study employs a state-of-art micro-PIV system which consists of epi-fluorescence microscope, 620nm diameter fluorescent seed particles and an 8-bit megapixel CCD camera. Velocity vector fields with a resolution of $6.8\;\times\;6.8{\mu}m$ has been obtained, and the attention has been paid on the effect of varying measurement conditions of particle diameter and particle concentration on the resulting PIV results. In this study, the microfluidic elements were fabricated on plastic chips by means of MEMS processes and a subsequent molding process. Flow fields in a variety of microchannels as well as micronozzle have been investigated.

• 한국가시화정보학회:학술대회논문집
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• 한국가시화정보학회 2004년도 Proceedings of 2004 Korea-Japan Joint Seminar on Particle Image Velocimetry
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• pp.111-118
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• 2004

A new micro-resolution PIV (Particle Image. Velocimetry) has been developed. To investigate transient phenomena in a microfluidic device, Dynamic micro-PIV system was realized by combining a high-speed camera and a CW(Continuous Wave) laser. The technique was applied to a micro-counter-current flow, consisting of water and butyl acetate. The velocity fields of water in the micro counter-current flow were visualized for a time resolution of 500 $\{mu}s$ and a spatial resolution of 2.2 x 2.2 $\{mu}m$. Using the Dynamic micro-PIV technique, the vortex-like motions of fluorescent particles at the water-butyl acetate interface were captured clearly

• 한국가시화정보학회지
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• 제13권2호
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• pp.16-21
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• 2015

Concentration polarization(CP) phenomena show great potential on desalination technology. As we can control the dimension of silicon based nanochannel system, it can be used to model ion exchange membrane. In this study, to investigate the effect of nanochannel number and working conditions on the CP based desalination process, nanochannel based microfluidic system is fabricated. First, we optimized nanochannel number and working conditions to conduct visualization study on CP based desalination process. Second, we visualized the desalination process with fluorescent dye in the desalination chip. We also visualized that the particles also can be removed by the CP based desalination process.

• 대한미생물학회지
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• 제3권1호
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• pp.51-65
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• 1968

The site of the synthesis of Japanese encephalitis virus(JEV) in the actinomycin-treated and infecter PS Y15 cells(a porcine kidney stable cell line) was observed by the immunofluorescent antibody technique, acridine orange staining, and the autoradiographic analysis. In the parallel studies by immunofluorescent technique and acridine orange staining it the infected cells, Viral protein(as an antigen) and viral RNA were detected at the same site of cytoplasm. In the autoradiographic analysis, the cytoplasmic labeling of $^3H$-uridine was due to the synthesis of JEV-RNA, while the nucleolus and nucleus were not involved. In the autoradiographic studies on the secton of infected cells, the $^3H$-uridine was frequently incorporated around the cytoplasmic vacuoles. This localization of labeling agreed with the site of acridine orange positive granules. The results suggest that the syntheses of the viral RNA and viral protein occurred in the similar site of cytoplasm of the infected cells, and also the virus particles seem to be assembled in the sites of the viral RNA and protein syntheses.

• 한국가시화정보학회지
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• 제21권3호
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• pp.65-74
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• 2023

Compared to epifluorescence(EPI) microscopy which captures fluorescence from the entire depth of sample, total internal reflection fluorescence(TIRF) can selectively visualize only a single surface of it. TIRF uses a thin evanescent field generated by the total internal reflection of laser light on surface. However, conventional TIRF system are designed for total internal reflection to occur at the upper surface of sample, making them unsuitable for sessile droplet imaging. We designed a TIRF system suitable for a sessile droplet imaging by utilizing slide glass as a lightguide. We presented the details for constructing the TIRF system using a prism, slide glass, air slit, and optical trap. Then, we compared the TIRF with EPI by imaging the droplet with fluorescent particles during its drying process. As a result, TIRF allows us to distinctly visualize the drying pattern on the bottom surface of droplet.

• 대한치과보철학회지
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• 제33권2호
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• pp.317-334
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• 1995

The purpose of this study was to compare the bone formation, maturation around HA-and titaniumcoated dental implants in dog. 5 hydroxyapatite coated IMZ implants and 5 titanium coated IMZ implants were placed into the previously extracted site in the mandible of 5 adults dogs. All dogs were injected intravenously Tetracycline, Alizalin red S, and Calcein for bone fluorescent labelling, After the experimental period of 16weeks, the dogs were sacrificed and tissue samples around the implants were obtained. Microscopic observations(ligth, polariged and fluorescence microscope), morphometric analysis, line profile with EPMA, and quantitative analysis for Ca,P, and Ti were performed. The results were as follows ; 1. Bone maturations around the implants were relatively lower than those of natural teeth. No significant differences in bone maturation and remodeling patterns were observed between the two implants groups. 2. Calcification of bone surrounding the implants was initiated in 8-11 weeks for HA-coated implants, while it took 11 weeks or more for Ti-coated implants. 3. Bone-to-implants contact ratio of 82.63% was recorded for HA-coated group and 72.25% for titanium coated group, with no significant difference between the two groups. 4. Bone around the implants exhibited reduced quantity of Ca and P in the $100{\mu}m$ region relative to natural teeth, while the rest of the regions showed no statistical differences. No significant differences were found between the two implant groups. 5. There was a separation of HA layer from the implant core and subsequent infiltration of inflammatory cells into the resulting space in the HA-coated implants, and evidences of phagocytosis of HA particles by macrophages. Bone calcification was more rapid around HA-coated implants compared to titanium-coated implants, but HA coated implants did not show any significant differences either in the degree of calcification or the bone-to-implant contact ratio over Ti coated implants. HA coated implants may have complications associated with HA absorption and separation of HA layer from the implant core.

• 한국의학물리학회:학술대회논문집
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• 한국의학물리학회 2002년도 Proceedings
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• pp.432-435
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• 2002

We have developed and proposed the heavy ion CT system which consists of fluorescent screen and CCD camera equipped with image intensifier. In our system, we have measured the residual range of particles that passed a phantom and reconstructed the CT image for the distribution of relative stopping power by filtered back projection method with Shepp '||'&'||' Logan filter. The heavy ion $\^$12/C accelerated up to 400 MeV/u by HIMAC (Heavy Ion Medical Accelerator in Chiba) was used. Intensity of the beam output changes like macro pulse, the period being 3.3 sec and the width being 2 sec. The series of data was acquired in synchronizing with the pulse, leading to the improvement of S/N in the CT image. The fundamental performance was experimentally evaluated in the proposed system. The spatial resolution was estimated to be about 1 mm and the density resolution (electron density referred to water) to be about 0.01.

• 대한기계학회논문집B
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• 제34권5호
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• pp.523-529
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• 2010

Micro actuator의 동력원으로 박테리아 주화성을 기반으로 한 편모박테리아 운동은 널리 연구되고 있다. 본 연구에서는 마이크로입자 추적유속계($\mu$-PTV)를 이용하여 박테리아 주화성에 의해 추진되는 형광입자의 움직임을 분석하였다. 일반적으로 활발한 운동성을 지니고 있는 편모 박테리아 중 Serratia marcescens가 배양액속에서 형광 폴리스티렌 미세입자 표면에 자발적으로 붙게 된다. 박테리아가 부착된 미세입자를 고형화된 화학적 유인물질 L-aspartate가 담겨져 있는 유체 속으로 주입하고, 시간에 따라 입자들이 서서히 L-aspartate가 높은 농도를 가지는 구역으로 이동하는 것을 관찰하였다. 본 연구의 결과로 편모박테리아가 micro actuator의 효율적인 동력원 개발에 적용될 수 있는 가능성을 제시하였다.