• Title/Summary/Keyword: Fluorescence method

검색결과 1,009건 처리시간 0.042초

리포솜 지질막의 성질에 미치는 지질 조성의 영향 (Effects of Lipid Composition on the Properties of Phospholipid Liposomal Membranes)

  • 김민;한석규;김종국
    • 약학회지
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    • 제38권2호
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    • pp.131-139
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    • 1994
  • Calcein-encapsulated small unilamellar vesicles of various lipid composition were prepared using the sonication technique, and their stabilities at $20^{\circ}C$ were examined by measuring calcein leakage from the liposomes. The fluidity of these liposomal bilayers was also investigated by measuring the fluorescence polarization of DPH labelled into the liposomes. The results showed that liposomes made of PC mixtures with different acyl chain length were very stable, which may be due to the formation of interdigitated bilayer structure. The addition of cholesterol further stabilized these PC liposomes. However, addition of cholesterol reduced the encapsulation efficiences of liposomes. The fluidity of the liposomes was significantly decreased by cholesterol in the liquid crystalline state, but not changed in the gel state. These results suggest that the enhanced stability of PC mixture liposomes may be ascribed to the formation of stable interdigitated bilayer structure. In membrane-mimetic and drug-delivery studies, vesicles made of mixtures of various phospholipids are recommended instead of addition of cholesterol to the phospholipid.

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직분식 가솔린기관 내에서 피스톤 형상이 연료혼합기 거동에 미치는 영향 (The Effect of Piston Bowl Shape on Behavior of Vapor Phase in a GDI Engine)

  • 황필수;강정중;김덕줄
    • 대한기계학회논문집B
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    • 제26권4호
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    • pp.614-621
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    • 2002
  • This study was performed to investigate the behavior of vapor phase of fuel mixtures with different piston bowl shapes(F, B and R-type) in a optically accessible engine. The images of liquid and vapor phases were captured in the motoring engine using exciplex fluorescence method. Fuel was injected into atmospheric nitrogen to prevent quenching phenomenon by oxygen. Injection pressure was 5.1MPa. Two dimensional spray fluorescence image of vapor phase was acquired to analyze spray behaviors and fuel distribution inside of cylinder. Four injection timings were set at BTDC 90$^{\circ}$, 80$^{\circ}$, 70$^{\circ}$, and 60$^{\circ}$. With a fuel injection timing of BTDC 90$^{\circ}$, fuel-rich mixture level in the center region was highest in a B-type piston. With a fuel injection timing of BTDC 60$^{\circ}$, R-type piston was best. R-type piston shape was suitable under enhanced swirl ratio and late injection condition and B-type piston shape was right in a weak swirl ratio. It was found that the piston bowl shape affected the mixture stratification inside of cylinder.

마이크로 채널 내부의 Micro-LIF 측정을 위한 마이크로 레이저 평면빔과 혼합용매의 적용 (Application of Micro-Thin Laser sheet and Mixed Solvent for Micro-LIF Measurement in a Microchannel)

  • 윤상열;김재민;김경천
    • 한국가시화정보학회:학술대회논문집
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    • 한국가시화정보학회 2004년도 추계학술대회 논문집
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    • pp.86-89
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    • 2004
  • One most feasible way to measure the concentration field in the micro-channel is using micro-LIF(Laser Induced Fluorescence) method. However, an accurate concentration field at a given cross plane in a micro-channel has not been successfully achieved so far due to various limitations in the light illumination and fluorescence signal detection. The present study demonstrates a novel method to provide an ultra thin laser sheet beam having five(5) microns thickness by use of a micro focus laser line generator. The laser sheet beam illuminates an exact plane of concentration measurement field to increase the signal to noise ratio and considerably reduce the depth uncertainty. Nile Blue A was used as fluorescent dye for the present LIF measurement. The enhancement of the fluorescent intensity signals was performed by a solvent mixture of water $(95\%)$ and ethanol (EtOH)/methanol (MeOH) $(5\%)$ mixture. To reduce the rms errors resulted from the CCD electronic noise and other sources, an expansion of grid size was attempted from $1\times1$ to 3(3 or 5(5 pixel data windows and the pertinent signal-to-noise level has been noticeably increased accordingly.

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피스톤 형상에 따른 직분식 가솔린기관 내에서의 연료혼합기 거동특성 연구 (The Effect of Piston Bowl Shape on Behavior of Vapor Phases in a GDI Engine)

  • 황필수;강정중;김덕줄
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2001년도 추계학술대회논문집B
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    • pp.915-920
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    • 2001
  • This study was performed to investigate the behavior of vapor phase of fuel mixtures with different piston bowl shapes(F, B, and R-type) in a optically accessible engine. The images of liquid and vapor phases were captured in the motoring engine using exciplex fluorescence method. Fuel was injected into atmospheric nitrogen to prevent quenching phenomenon by oxygen. Injection pressure is 5.1MPa. Two dimensional spray fluorescence image of vapor phases was acquired to analyze spray behaviors and fuel distribution inside of cylinder. Four injection timings were set at BTDC $90^{\circ},\;80^{\circ},\;70^{\circ},\;and\;60^{\circ}$. With a fuel injection timing of BTDC $90^{\circ}$, fuel-rich mixture level in the center region was highest in a B-type piston. With a fuel injection timing of BTDC $60^{\circ}$, R-type piston was best. R-type piston shape was suitable under enhanced swirl ratio and late injection condition and B-type piston shape was right in a weak swirl ratio. It was found that the piston bowl shape affected the mixture stratification inside of cylinder.

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직분식 가솔린 엔진에서 피스톤 캐비티 반경에 따른 연료 거동 분석 (A Study on the Fuel Behaivor with Cavity Diameter in a Gasoline Direct Injection Engine)

  • 김태안;강정중;김덕줄
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2003년도 추계학술대회
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    • pp.472-477
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    • 2003
  • This study was performed to investigate the behavior of vapor phase of fuel mixtures with different piston cavity diameters in a optically accessible engine. The images of vapor phases were measured in the motoring engine using exciplex fluorescence method. The conventional engine was modified as GDI engine with swirl flow. Fuel was injected into atmospheric nitrogen to prevent quenching phenomenon by oxygen. Injection pressure is 5.1MPa. Two dimensional spray fluorescence image of vapor phases was acquired to analyze spray behavior and fuel distribution inside of cylinder. Three injection timings were set at BTDC $180^{\circ}$, $60^{\circ}$and $60^{\circ}$. With a fuel injection timing of BTDC $60^{\circ}$, fuel-rich mixture was concentrated in near the cavity center. With a fuel injection timing of BTDC $60^{\circ}$, fuel-rich mixture level in the center region was highest in the S-type during the late compression stroke. With a fuel injection timing of BTDC $180^{\circ}$, fuel was not affected in a piston cavity and generally distributed as homogeneous mixture.

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분무각이 다른 GDI인젝터에 대한 증발특성 (Vaporizing Characteristics of Spray from Two Different GDI Injectors)

  • 최동석;김덕줄
    • 대한기계학회논문집B
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    • 제25권5호
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    • pp.688-696
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    • 2001
  • Vaporizing characteristics of two GDI injectors with different spray angles were investigated using exciplex fluorescence method. Injector I has narrower spray angle, while injector II has wider one. The exciplex system of fluorobenzene and DEMA in a non-fluorescing base fuel of hexane was employed. In quantifying concentration of fuel vapor, quenching of concentration and temperature was corrected. Droplet size and velocity were also measured by PDPA under non-vaporizing condition. From obtaining the images of liquid and vapor phases, vaporizing GDI sprays could be divided as two regions: cone and mixing regions. For injector I, vortex region was not developed. High concentration of fuel vapor due to vaporization of many fine droplets was distributed near the spray axis. For injector II, droplets with the diameter of about 10 $\mu$m were distributed in the vortex region. The vortex region had high concentration of fuel vapor due to vaporization of these droplets. Particularly, higher and lower concentrations of fuel vapor were balanced at 2ms after the start of injection for injector II.

직분식 엔진에서 실린더 내 연료의 액.기상 거동에 미치는 텀블과 스월의 영향 (The Effects of Tumble and Swirl Flow on the Behavior of Liquid/Vapor Phases in a DI Gasoline Engine)

  • 강정중;최동석;김덕줄
    • 한국자동차공학회논문집
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    • 제10권2호
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    • pp.23-30
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    • 2002
  • This present study experimentally investigates the behavior of liquid and vapor phase of fuel mixtures with changing the in-cylinder air motion in an optically accessible engine. The conventional MPI/DOHC engine was modified to gasoline direct injection engine with swirl motion. The images of liquid and vapor phases were captured in the motoring operation condition using exciplex fluorescence method. Two dimensional spray fluorescence images of liquid and vapor phases were acquired to analyze spray behaviors and fuel distribution inside of cylinder respectively, In early injection timings $(BTDC\;270^{\circ},\;180^{\circ})$, tumble flow transported most of vapor phase to the lower region and the both sides of cylinder, so vapor phase didn't become uniform distribution up to the half of the compression stroke. In the case of swirl flow, the fuel mixture was confined near the swirl origin in upper region of cylinder. In late injection timings $(BTDC\;90^{\circ})$, tumble flow transported vapor phase to the intake valve and swirl flow to the exhaust valve.

LIF를 이용한 Hg-Ar 플라즈마 특성 평가 (Evaluation of Plasma Characteristics for Hg-Ar Using LIF)

  • 문종대
    • 전기학회논문지P
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    • 제57권2호
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    • pp.79-83
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    • 2008
  • In this paper, we introduced a LIF measurement method and summarized the theoretical side. When an altered wavelength of laser and electric power, lamp applied electric power, we measured the relative density of the metastable state in mercury after observing a laser induced fluorescence signal of 404.8nm and 546.2nm, and confirmed the horizontal distribution of plasma density in the discharge lamp. Due to this generation, the extinction of atoms in a metastable state occurred through collision, ionization, and excitation between plasma particles. The density and distribution of the metastable state depended on the energy and density of plasma particles, intensely. This highlights the importance of measuring density distribution in plasma electric discharge mechanism study. The results confirmed the resonance phenomenon regarding the energy level of atoms along a wavelength. change, and also confirmed that the largest fluorescent signal in 436nm, and that the density of atoms in 546.2nm ($6^3S_1{\to}6^3P_2$) were larger than 404.8nm ($6^3S_1{\to}6^3P_2$). According to the increase of lamp applied electric power, plasma density increased, too. When increased with laser electric power, the LIF signal reached a saturation state in more than 2.6mJ. When partial plasma density distribution along a horizontal axis was measured using the laser induced fluorescence method, the density decreased by recombination away from the center.

형광(螢光)을 이용한 불량식품(不良食品)의 감별(鑑別) - 식품(食品)의 발광(發光) 패턴 - (Detection of Adulterated Foods by the Use of Fluorescence)

  • 이미순
    • 한국식품과학회지
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    • 제10권1호
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    • pp.16-26
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    • 1978
  • UVSL-25 mineralight를 조사(照射)한 경우에 발생하는 식품자체(食品自體)의 형광(螢光)을 이용하여 Adulteration이나 변질(變質)에 의한 불량식품(不良食品)을 감별(鑑別)할 수 있는 방법확립(方法確立)을 시도(試圖)해 보았다. 형광(螢光)의 육안관찰(肉眼觀察) 및 표면발광(表面發光)의 스펙트럽분석(分析)은 본 실험조건(實驗條件)하에서는 식품감별방법(食品鑑別方法)으로 부적당하게 보였다. 유동(流動)파라핀에 현탁(懸濁)된 분말물질(粉末物質), 또는 액체(液體) 시료(試料)의 흡수(吸收) 및 발광(發光)스펙트럼은 식품(食品)의 종류에 따라 특징적인 패턴을 나타냈다. 재현성(再現性)있는 실험결과(實驗結果)를 얻기위해서는 시료(試料)의 균일성(均一性)이 가장 중요한 요인으로 보였다.

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The optical analysis of discharge lamp by Laser

  • Yang, Jong-Kyung;Lee, Jong-Chan;Choi, Yong-Sung;Park, Dae-Hee
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2005년도 하계학술대회 논문집 Vol.6
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    • pp.570-571
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    • 2005
  • In this paper, we introduced a LIF measurement method and summarized the theoretical side. When an altered wavelength of laser and electric power, lamp applied electric power, we measured the relative density of the metastable state in mercury after observing a laser induced fluorescence signal of 404.8nm and 546.2nm, and confirmed the horizontal distribution of plasma density in the discharge lamp. The results confirmed the resonance phenomenon regarding the energy level of atoms along a wavelength change, and also confirmed that the largest fluorescent signal in 436nm, and that the density of atoms in 546.2nm ($6^3S_1\rightarrow6^3P_2$) were larger than 404.8nm ($6^3S_1\rightarrow6^3P_1$). According to the increase of lamp applied electric power, plasma density increased, too. When increased with laser electric power, the L1F signal reached a saturation state in more than 2.6mJ. When partial plasma density distribution along a horizontal axis was measured using the laser induced fluorescence method, the density decreased by recombination away from the center.

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