• Proceedings of the KSME Conference
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• 2007.05b
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• pp.2585-2590
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• 2007

In this paper, a technique of simultaneously measuring the velocity and the temperature in micro-scale flow is proposed. This method uses particle tracking velocimetry (PTV) for measuring the velocity and laser induced fluorescence (LIF) for measuring the temperature. To measure the accurate velocity and temperature, images for PTV and for LIF are separated by using two light sources and a shutter which is synchronized with a camera. By using only one camera, measurement system can be simplified and the error from complicate optical system can be minimized. Error analyses regarding the concentrations of fluorescent dye and particle and the light source fluctuation are also conducted. It is found that the error of the temperature and the velocity highly depends on the concentration of fluorescent particles which are used for PTV. This technique is applied to the simultaneous measurement of the velocity and the temperature in the electrokinetic flow. It is found that the velocity and temperature vary with the electric field strength and the concentration of electrolyte.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.31 no.7 s.262
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• pp.644-652
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• 2007

In this paper, a technique of simultaneously measuring the velocity and the temperature in micro-scale flow is proposed. This method uses particle tracking velocimetry (PTV) for measuring the velocity and laser induced fluorescence (LIE) for measuring the temperature. To measure the accurate velocity and temperature, images for PTV and for LIE are separated by using two light sources and a shutter which is synchronized with a camera. By using only one camera, measurement system can be simplified and the error from complicate optical system can be minimized. Error analyses regarding the concentrations of fluorescent dye and particle and the light source fluctuation are also conducted. It is found that the error of the temperature and the velocity highly depends on the concentration of fluorescent particles which are used for PTV. This technique is applied to the simultaneous measurement of the velocity and the temperature in the electrokinetic flow. It is found that the velocity and temperature vary with the electric field strength and the concentration of electrolyte.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.24 no.10
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• pp.1399-1408
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• 2000

A 2-dimensional temperature field measurement technique using PLIF (Planar Laser Induced Fluorescence) was developed and it was applied to an axisymmetric buoyant jet. Rhodamine B was used as a fluorescent dye. Laser light sheet illuminated a two-dimensional cross section of the jet. The intensity variations of LIF signal from Rhodamine B molecules scattered by the laser light were captured with an optical filter and a CCD camera. The spatial variations of temperature field of buoyant jet were derived using the calibration data between the LIF signal and real temperature. The measured results show that the turbulent jet is more efficient in mixing compared to the transition and laminar jet flows. As the initial flow condition varies from laminar to turbulent flow, the entrainment from ambient fluid increases and temperature decay along the jet center axis becomes larger. In addition to the mean temperature field, the spatial distributions of temperature fluctuations were measured by the PLIF technique and the result shows the shear layer development from the jet nozzle exit.

• Journal of the Optical Society of Korea
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• v.19 no.3
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• pp.228-236
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• 2015

Fluorescence recovery after photobleaching (FRAP) is a well-established method commonly used to measure the diffusion of fluorescent solutes and biomolecules in living cells or tissues. Here a fiber-optic-based FRAP (f-FRAP) system was developed, and validated using macromolecules in water and agarose gels of different concentrations. We applied f-FRAP to measure the site-specific diffusion of fluorescein (NaFluo) in peritoneal membranes (PMs) on the liver, cecum, and kidney of a living rat during peritoneal dialysis. Diffusion of fluorescein in PM varied in a time-dependent manner according to the type of organ ($D_{PM\;on\;Liver}/D_{NaFluo}=0.199{\pm}0.085$, $D_{PM\;on\;Cecum}/D_{NaFluo}=0.292{\pm}0.151$, $D_{PM\;on\;Kidney}/D_{NaFluo}=0.218{\pm}0.110$). The proposed method allows direct quantitative measurement of the three-dimensional diffusion in local PM in vivo, which was previously inaccessible by peritoneal function test methods such as peritoneal equilibration test (PET) and standardized PM assessment (SPA). f-FRAP is promising for local and dynamic assessments of peritoneal pathophysiology and the mass transport properties of PMs, presumed to be affected by variation of tissue structures over different organs and functional changes of the PM with years of peritoneal dialysis.

• Current Optics and Photonics
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• v.5 no.3
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• pp.229-237
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• 2021

The capabilities of the near-field scanning optical microscope (NSOM) for obtaining high resolution lateral topographical images as well as for mapping the spectroscopic and optical properties of a sample below the diffraction limit of light have made it an attractive research field for most researchers dealing with optical characteristics of materials in nano scales. The apertured NSOM technique involves confining light into an aperture of sub-wavelength size and using it to illuminate a sample maintained at a distance equal to a fraction of the sub-wavelength aperture (near-field region). In this article, we present a setup for developing NSOM using a cantilever with a sub-wavelength aperture at the tip. A single laser is used for both cantilever deflection measurement and near-field sample excitation. The laser beam is focused at the apex of the cantilever where a portion of the beam is reflected and the other portion goes through the aperture and causes local near-field optical excitation of the sample, which is then raster scanned in the near-field region. The reflected beam is used for an optical beam deflection technique that yields topographical images by controlling the probe-sample in nano-distance. The fluorescence emissions signal is detected in far-field by the help of a silicon avalanche photodiode. The images obtained using this method show a good correlation between the topographical image and the mapping of the fluorescence emissions.

• Korean Journal of Environmental Agriculture
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• v.1 no.1
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• pp.48-52
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• 1982

Methods for the measurement of aquatic bacteria can be divided into two groups. The first group of these methods is based on the 'replicon' concept that live bacterial cells, when diluted and transferred to a suitable medium, produce colonies. These methods distinguish living from dead bacteria, but they massively underestimate bacterial numbers. The second group of enumeration methods uses visual counting technique using specific apparatus such as a microscope. These methods are generally direct and simple, but it is very hard to distinguish between live and dead bacteria and between small particle and bacteria. Recently developed technique in staining methods has provided a reliable method of visual determination of aquatic bacteria. This uses epifluorescence microscopy to measure the total bacterial population. In order to present the fluorescence microscopy as a new methodology for the determination of bacterial numbers in water supplies, data were obtained from chlorine and monochloramine doses added to samples. Total counts by fluorescence microscopy were compared with standard plate count method. The total number of bacteria in water supplies can be determined with fluorescence microscopy. This technique allows better resolution of small bacteria and differentiation of particle from bacteria. Chloramine was found to persist longer in natural waters and prevent bacterial regrowth.

• Journal of Integrative Natural Science
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• v.1 no.1
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• pp.1-4
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• 2008

We developed quantification method based on the concept of candela in physics. The measurement of fluorescence signal from a nude mouse in the research of molecular biology. In the measurement of the optical signal with CCD, the quantification method for photon counts based on bio-luminescence imaging technique can provide comparative reference data. In this paper, we described theoretical derivation of our proposed concept. We hope this method could be a useful standard reference for quantitative date analysis in optical imaging.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2001.07a
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• pp.757-760
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• 2001

We have used the random fluidic self-assembly (RFSA) technique based on the chip pattern of hydrophobic self-assembly layers to assemble microfabricated particles onto the chip pattern. Immobilization of DNA, fabrication of the particles and the chip pattern, arrangement of the particles on the chip pattern, and recognition of each using DNA fluorescence measurement were carried out. Establishing the walls, the arrangement stability of the particles was improved. Each DNA is able to distinguish by using the lithography process on the particles. Advantages of this method are process simplicity, wide applicability and stability. It is thought that this method can be applicable as a new fabrication technology to develop a minute integration type biosensor microarray.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.4
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• pp.504-510
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• 2003

Simultaneous measurements of turbulent velocity and concentration field in a stirred mixer tank are carried out by using PIV/LIF technique. Instantaneous velocity fields are measured with a 1K$\times$1K CCD camera adopting the frame straddle method while the concentration fields are obtained by measuring the fluorescence intensity of Rhodamine B tracer excited by the second pulse of Nd:Yag laser light. Image distortion due to the camera view-angle is compensated by a mapping function. It is found that the general features of the mixing pattern are quite dependent on the local flow characteristics during the rapid decay of mean concentration. However, the small scale mixing seems to be independent on the local turbulent velocity fluctuation.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2006.11a
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• pp.59-63
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• 2006

A direct photograph measurement technique was used to determine the spatial distribution of the spray droplet diameter in subsonic crossflow and it also obtain that SMD distribution by using PLLIF technique. The injector internal flow was classified as three modes such as a normal, cavitation, and hydraulic flip. The objectives of this research are getting a droplet distribution and drop size measurement of normal flow and compare with the other flow effects. Although the study showed visually that drop size were spatially dependent of Air-stream velocity, fuel injection velocity, and normalized distance from the injector exit length.(x/d, y/d) There are also difference characteristics between cavitation, hydraulic flip and the normal flow.