• 제목/요약/키워드: Fluorescence measurement

검색결과 319건 처리시간 0.029초

라디칼 PLIF계측을 이용한 연소실의 공간적 열발생율 예측 (Prediction of Spatial Heat Release Rate of Combustion Chamber by Radicals-PLIF)

  • 최경민
    • 한국분무공학회지
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    • 제8권4호
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    • pp.9-16
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    • 2003
  • The Purpose of this study is to investigate the relationships between the local heat release rate and CH concentration have been investigated by numerical simulations of methane-air premixed flames. And simultaneous CH and OH PLIF(Planar Laser Induced Fluorescence) measurement has been also conducted for lean premixed flame as well as for laminar flames. Numerical simulations are conducted for laminar premixed flames and turbulent ones by using PREMIX in CHEMKIN and two dimensional DNS code with GRI mechanism version 2.11, respectively. In the case of laminar premixed flame, the distance between the peak of heat release rate and that of CH concentration is under $91{\mu}m$ for all equivalence ratio calculated in present work. Even for the premixed flame in high intensity turbulence, the distribution of the heat release rate coincides with that of CH mole fraction. For CH PLIF measurements in the laminar premixed flame burner, CH fluorescence intensity as a function of equivalence ratio shows a similar trend with CH mole fraction computed by GRI mechanism. Simultaneous CH and OH PLIF measurement gave us useful information of instantaneous reaction zone. In addition, CH fluorescence can be measured even for lean conditions where CH mole fraction significantly decreases compared with that of stoichiometric condition. It was found that CH PLIF measurements can be applicable to the estimation of the spatial fluctuations of heat release rate in the engine combustion.

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형광 나노 포러스 박막을 이용한 표면 온도 센서의 제작 및 성능 연구 II (Fabrication and Performance Investigation of Surface Temperature Sensor Using Fluorescent Nanoporous Thin Film II)

  • 김현정;유재석;박진일
    • 설비공학논문집
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    • 제25권12호
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    • pp.674-678
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    • 2013
  • We present a non-invasive technique to the measure temperature distribution in nano-sized porous thin films by means of the two-color laser-induced fluorescence (2-LIF) of rhodamine B. The fluorescence induced by the green line of a mercury lamp with the makeup of optical filters was measured on two separate color bands. They can be selected for their strong difference in the temperature sensitivity of the fluorescence quantum yield. This technique allows for absolute temperature measurements by determining the relative intensities on two adequate spectral bands of the same dye. To measure temperature fields, Silica (SiO2) nanoporous structure with 1-um thickness was constructed on a cover glass, and fluorescent dye was absorbed into these porous thin films. The calibration curves of the fluorescence intensity versus temperature were measured in a temperature range of $10-60^{\circ}C$, and visualization and measurement of the temperature field were performed by taking the intensity distributions from the specimen for the temperature field.

형광 나노포러스 박막의 온도장 가시화 (The Visualization of Temperature Field for Nanoporous Thin Film using Laser-Induced Fluorescence.)

  • 오영수;백인기;전필수;김현정;유재석
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2007년도 춘계학술대회B
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    • pp.2459-2464
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    • 2007
  • In present study, a temperature field of specimens which was coated with fluorescence dye such as Rhodamine-B(Rh-B) has been measured, based on the fluorescence intensity. Silica(SiO2) nano porous structure with 1um thickness was constructed on a cover glass, and fluorescence dye was digested into these porous thin films. To optimize manufacturing coating process, various solvents, Rh-B concentration, and other chemical materials were applied to fabricate the specimen and all specimens were measured on the various temperature conditions. For the measurement, a 14 bit cooled CCD camera with 1600 by 1200 spatial resolution is equipped with epifluorescence microscope to obtain only fluorescence intensity from 1.2 mm by 0.9 mm field of view of the illuminated coated specimen.

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복수 경로를 지닌 자외선 형광측정기를 이용한 오일 산화도 측정장치 (Apparatus for Monitoring Oil Oxidation Using a Plurality of UV Fluorescence Light-reflecting Members)

  • 공호성;한흥구
    • Tribology and Lubricants
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    • 제26권3호
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    • pp.167-174
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    • 2010
  • An apparatus for measuring oil oxidation was developed, which is capable of being mounted to mechanical devices for detecting power of fluorescent light reflected from oil in real time as an indication of the oil oxidation. This device has an advantage over conventional fluorescence spectrometers where the thin film is required for the measurement. Clean and used oil samples (mineral and synthetic oils) were tested by the developed apparatus that calculates a fluorescence quantum yield and a light absorption coefficient of the oil based upon the signals from the two light-receiving members and evaluates the degree of oil oxidation of test oils based on the fluorescence quantum yield. Results generally show that the developed device is able to effectively evaluate oil oxidation characteristics on-site in the field.

마이크로 채널 내부의 Micro-LIF 측정을 위한 마이크로 레이저 평면빔과 혼합용매의 적용 (Application of Micro-Thin Laser sheet and Mixed Solvent for Micro-LIF Measurement in a Microchannel)

  • 윤상열;김재민;김경천
    • 한국가시화정보학회:학술대회논문집
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    • 한국가시화정보학회 2004년도 추계학술대회 논문집
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    • pp.86-89
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    • 2004
  • One most feasible way to measure the concentration field in the micro-channel is using micro-LIF(Laser Induced Fluorescence) method. However, an accurate concentration field at a given cross plane in a micro-channel has not been successfully achieved so far due to various limitations in the light illumination and fluorescence signal detection. The present study demonstrates a novel method to provide an ultra thin laser sheet beam having five(5) microns thickness by use of a micro focus laser line generator. The laser sheet beam illuminates an exact plane of concentration measurement field to increase the signal to noise ratio and considerably reduce the depth uncertainty. Nile Blue A was used as fluorescent dye for the present LIF measurement. The enhancement of the fluorescent intensity signals was performed by a solvent mixture of water $(95\%)$ and ethanol (EtOH)/methanol (MeOH) $(5\%)$ mixture. To reduce the rms errors resulted from the CCD electronic noise and other sources, an expansion of grid size was attempted from $1\times1$ to 3(3 or 5(5 pixel data windows and the pertinent signal-to-noise level has been noticeably increased accordingly.

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신속한 적조 예찰이 가능한 형광 측정시스템 개발 (Development of a Fluorescence Measurement System Capable of Rapid Red Tide Monitoring)

  • 백경훈;오영지;조현서;강윤자;이준석
    • 센서학회지
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    • 제33권1호
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    • pp.30-33
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    • 2024
  • The occurrence of harmful algae on the coast of Korea has been a cause of damage to the aquaculture industry and deterioration of the coastal ecosystem environment. A method is required to predict their outbreak in real-time at the site. Therefore, this study attempted to develop a small hybrid optical sensor and real-time monitoring system based on LiDAR that can be used in the field and laboratory and can be applied to various platforms. FMS-L specifically suggested the amount of Chlorophyll a (Chl a) in the sample by measuring and analyzing the fluorescence emitted by the irradiating light. The accuracy of FMS-L was verified by measuring the concentrations of standard Chlorophyll a substances and Margalfidinium polykirkoids. In addition, the precision was verified by comparing the measurement results of FMS-L using commercial equipment Phyto-PAM-II. This equipment is compact and easy to move. Therefore, it can be easily applied to field surveys, allows short time measurements (10 s), and can be applied at a distance of 10 m from the measurement site.

대기중 이산화황 관측 방법들의 실측 비교 연구 (An Intercomparison of Atmospheric SO$_{2}$ Measurement Techniques)

  • 김경렬;이동수;이강웅
    • 한국대기환경학회지
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    • 제12권5호
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    • pp.587-591
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    • 1996
  • An intercomparison experiment was implemented to assess the uncertainties and precisions om atmospheric SO$_{2}$ measurement techiques including a pulsed fluorescence (P-F), a diffusion-scrubber /ion chromatography (D-I), and a mist-chamber/ion chromatography (M-I). Each of those three techniques was investigated by researchers at Seoul National University, Yonsei University, and Hankuk University of Foreign Studies, respectively. The concentrations of atmospheric SO$_{2}$ were determined concurrently using three independent measurement techniques at the Seoul National University campus, Korea during Nov.22 to Dec.2, 1995. While the results from the P-F and D-I techinques showed the very close agreements (within +-5%) throughout the experiment period, M-I technique showed systematically smaller values (up to 30%) than the other two techniques. Although sources of larger discrepancy between different techniques were mot identified, the lower SO$_{2}$ values of the M-I method may be related to the errors associated with sample collection effciency, mass flow rate measurements, and standardization of ion chromatography.

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Hyperspectral Fluorescence Imaging for Mouse Skin Tumor Detection

  • Kong, Seong G.;Martin, Matthew E.;Vo-Dinh, Tuan
    • ETRI Journal
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    • 제28권6호
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    • pp.770-776
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    • 2006
  • This paper presents a hyperspectral imaging technique based on laser-induced fluorescence for non-invasive detection of tumorous tissue on mouse skin. Hyperspectral imaging sensors collect image data in a number of narrow, adjacent spectral bands. Such high-resolution measurement of spectral information reveals contiguous emission spectra at each image pixel useful for the characterization of constituent materials. The hyperspectral image data used in this study are fluorescence images of mouse skin consisting of 21 spectral bands in the visible spectrum of the wavelengths ranging from 440 nm to 640 nm. Fluorescence signal is measured with the use of laser excitation at 337 nm. An acousto-optic tunable filter (AOTF) is used to capture images at 10 nm intervals. All spectral band images are spatially registered with the reference band image at 490 nm to obtain exact pixel correspondences by compensating the spatial offsets caused by the refraction differences in AOTF at different wavelengths during the image capture procedure. The unique fluorescence spectral signatures demonstrate a good separation to differentiate malignant tumors from normal tissues for rapid detection of skin cancers without biopsy.

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유기용매 중에서 Chlorophyll-b의 흡광 및 형광 (제2보) (The Absorbance and Fluorescence of Chlorophyll-b in Organic Solvents (II))

  • 이중화;김명숙;정구춘;박면용
    • 대한화학회지
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    • 제26권4호
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    • pp.224-228
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    • 1982
  • chl-b를 소중합체로서 용매에 녹였을 때 친핵성 n-prOH를 첨가함에 따라 흡광도와 fluorescence emission의 세기는 증가하였지만 단위체로 녹은 다음에 n-prOH의 농도증가에 따라 점차 감소하였다. chl-b는 소중합체의 생성때문에 Beer 법칙에 따르지 않았으며 용매의 극성변화에 따라 stockes shift를 나타냈다. 이러한 경향성은 chl-b의 정량에 고려하여야될 점이다.

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UV-Vis 분광분석에 의한 전기변압기 내 절연지 열화도 측정 (Application of UV-Vis Spectroscopic Analysis for Transformer Insulating Paper Degradation)

  • 공호성;한흥구
    • Tribology and Lubricants
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    • 제35권3호
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    • pp.151-157
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    • 2019
  • Insulated oil degradation produces charged by-products, such as acids and hydro-peroxides, which tend to reduce the insulating properties of the oil. In this study, UV-vis spectroscopy measurement technology is developed and experimentally compared with other measurement methods, such as the titration method and IR spectroscopy, to validate its ability to monitor the degradation of electrical insulating paper. The degradation characteristics of the insulating paper are appropriately represented through various types of measurement methods, such as the Tan (delta) method, $CO_2$ gas production measurement, the titration method, and IR spectroscopy. The results are demonstrated to be well comparable to a change in the fluorescence emission ratio (FER), which is defined as the shift in fluorescence intensity in the measured wavelength range, and also to the chromatic ratio, which is defined as a color shift to longer wavelength ranges. The results also show that, by using UV-vis spectroscopy, it is possible to detect the degradation of the insulating paper. This study suggests that UV-vis spectroscopy can be applied as an alternative to high-performance liquid chromatography, which is the internationally recognized measurement technology for cellulose paper degradation. The FER detector is also verified to be useful as an effective condition-monitoring device for power transformers.