• Title/Summary/Keyword: Fluorescence measurement

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Experimental Methodology Development for SFR Subchannel Analysis Code Validation with 37-Rods Bundle (소듐냉각고속로 부수로 해석코드 검증을 위한 37봉다발 실험방법 개념 개발)

  • Euh, Dong-Jin;Chang, Seok-Kyu;Bae, Hwang;Kim, Seok;Kim, Hyung-Mo;Choi, Hae-Seob;Choi, Sun-Rock;Lee, Hyung-Yeon
    • The KSFM Journal of Fluid Machinery
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    • v.17 no.6
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    • pp.89-94
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    • 2014
  • The 4th generation SFR is being designed with a milestone of construction by 2028. It is important to understand the subchannel flow characteristics in fuel assembly through the experimental investigations and to estimate the calculation uncertainties for insuring the confidence of the design code calculation results. The friction coefficient and the mixing coefficient are selected as primary parameters. The two parameters are related to the flow distribution and diffusion. To identify the flow distribution, an iso-kinetic method was developed based on the previous study. For the mixing parameters, a wire mesh system and a laser induced fluorescence methods were developed in parallel. The measuring systems were adopted on 37 rod bundle test geometry, which was developed based on the Euler number scaling. A scaling method for a design of experimental facility and the experimental identification techniques for the flow distribution and mixing parameters were developed based on the measurement requirement.

Interaction of Native and Apo-carbonic Anhydrase with Hydrophobic Adsorbents: A Comparative Structure-function Study

  • Salemi, Zahra;Hosseinkhani, Saman;Ranjbar, Bijan;Nemat-Gorgani, Mohsen
    • BMB Reports
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    • v.39 no.5
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    • pp.636-641
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    • 2006
  • Our previous studies indicated that native carbonic anhydrase does not interact with hydrophobic adsorbents and that it acquires this ability upon denaturation. In the present study, an apo form of the enzyme was prepared by removal of zinc and a comparative study was performed on some characteristic features of the apo and native forms by far- and near-UV circular dichroism (CD), intrinsic fluorescent spectroscopy, 1-anilino naphthalene-8-sulfonate (ANS) binding, fluorescence quenching by acrylamide, and Tm measurement. Results indicate that protein flexibility is enhanced and the hydrophobic sites become more exposed upon conversion to the apo form. Accordingly, the apo structure showed a greater affinity for interaction with hydrophobic adsorbents as compared with the native structure. As observed for the native enzyme, heat denaturation of the apo form promoted interaction with alkyl residues present on the adsorbents and, by cooling followed by addition of zinc, catalytically-active immobilized preparations were obtained.

The Effects of Operating Conditions and Injector Geometry on the Spray Characteristics of Swirl Injectors (스월 인젝터의 작동조건 및 인젝터 형상에 따른 분무특성)

  • Kim, D.J.;Im, J.H.;Han, P.G.;Yoon, Y.B.
    • Journal of ILASS-Korea
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    • v.9 no.1
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    • pp.21-29
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    • 2004
  • The flow characteristics of a swirl injector were investigated with the variation of the flow condition and geometric dimensions, such as orifice length for considering the viscous effect and tangential entry port area for considering the swirl intensity. The liquid film thickness strongly influencing on the formed drop size of the spray was measured using a new technique. The film thickness measurement technique proposed here, used the attenuation of fluorescence signal near the injector exit. The breakup length that is important for the flame location as well as the spray cone angle which influences on the ignition performance was measured using a backlit stroboscopic photography technique. From the experimental results, it is found that an increase in injection pressure decreased the film thickness and breakup length, and also enlarged the spray cone angle. A decrease in orifice length and tangential entry port area has a similar tendency of thinner film thickness, shorter breakup length and larger spray cone angle. In the present study, we proposed empirical models of the flow characteristics of the swirl injectors.

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Synthesis and Property of Pyrene-Naphthalene Diimide-Pyrene Triad (Pyrene-Naphthalene Diimide-Pyrene Triad의 합성 및 물성에 대한 연구)

  • Kim, Hyunji;Kim, A-Rong;Park, Jong S.
    • Textile Coloration and Finishing
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    • v.26 no.4
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    • pp.305-310
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    • 2014
  • In this study, we presented a newly synthesized pyrene-naphthalene diimide(NDI)-pyrene triad. The optical and structural properties were examined using various characterization techniques. A donor-acceptor-donor triad molecule exhibited a strong charge transfer, though there existed neither intramolecular nor intermolecular hydrogen bonding sites, due to the formation of preferential complementary complex between pyrene and NDI. Powder XRD measurement revealed a sharp and distinctive X-ray patterns, indicating the presence of microcrystalline-like structure. POM images showed anisotropic fingerprint texture similar to that of cholesteric phase, and SEM images showed numerous columnar structures with length of 1 to $10{\mu}m$. Above observation clearly demonstrated that ${\pi}$-complementary NDI-pyrene interactions in the traid was strong enough to form columnar aggregates in the long range.

Basic Study on the Spatial Structure Analysis of the Evaporative Diesel Spray (증발디젤분무의 공간적 구조해석에 관한 기초 연구)

  • Yeom, J.K.
    • Journal of Power System Engineering
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    • v.14 no.3
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    • pp.5-12
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    • 2010
  • The purpose of this study is to analyze heterogeneous distribution of branch-like structure at downstream region of inner spray. The previous many studies about diesel spray structure have yet stayed in the analysis of 2-D structure, and there are very few of informations which are concerned with 3-D analysis of the structure. The heterogeneous distribution of droplets in inner spray affects the mixture formation of diesel spray, and also the combustion characteristics of the diesel engines. Therefore, in order to investigate 3-D structure of evaporative spray the laser beam of 2-D plane was used in this study. Liquid fuel was injected from a single-hole nozzle (l/d=5) into a constant-volume vessel under high pressure and temperature in order to visualize the spray phenomena. The incident laser beam was offset on the central axis. From the images analysis taken by offset of laser beam, we examine formation mechanism of heterogeneous distribution by vortex flow at the downstream of the diesel spray. As the experimental results, the branch-like structure formed heterogeneous distribution of the droplets consists of high concentration of vapor phase in the periphery of droplets and spray tip of branch-like structure. Also the 3-D spatial structure of the evaporative diesel spray can be verified by images obtained from 2-D measurement methods.

A Basic study on the Evaporative Diesel Spray with Visible Measurement (가시화 측정을 이용한 증발디젤분무의 기초 연구)

  • Yeom, J.K.
    • Journal of Power System Engineering
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    • v.13 no.3
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    • pp.20-26
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    • 2009
  • 디젤기관에서 배출되는 유해배출성분인 NOx(Nitrogen oxides)와 PM(Particulate matter)은 기관 실린더내의 혼합기 분포에 의해 그 생성이 지배된다. 이 때문에 그 유해배출물들을 저감하기 위해서는 연소의 전단계인 혼합기 분포 및 그 생성과정의 해석은 매우 중요하다. 디젤기관에서 노즐로부터 분사된 연료는 주위기체와 혼합기를 형성하는 과정에서 액체에서 기체로 상변화를 동반한다. 따라서 분무의 혼합기형성과정을 해석하기 위해서는 액상과 기상을 동시에 분리하여 계측하는 것이 필요하다. 그러므로 본 연구에서는 디젤분무를 대상으로 Melton 등이 제안한 엑시플렉스(Exciplex) 형광법을 이용하여, 분무의 액상과 기상을 동시에 2차원분리해서 가시화촬영을 행하였다. 그 엑시플렉스 형광법을 이용하여 획득한 이미지에 화상 응용해석을 실시하여 비정상증발디젤분무의 혼합기형성과정에 대한 정보를 얻고자 하였다. 엑시플렉스 형광법을 이용해서 증발분무의 거동측성을 해석한 결과 프랙틸해석을 이용한 분무 흐트러짐(Disturbance)의 평가에서 플랙틸차원은 분사압력의 변화에 관계없이 하나의 값, 약 1.1로 정리 할 수 있고, 그 결과 각 분사압력에 대한 분무 기상외곽곡선(외주)은 거의 동일한 정도의 요철형상을 갖는다.

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Measurement of Cross-sectional Temperature Distribution in Micro-scale Gap Fluid Using LIF Technique in Combination with CLSM (LIF 및 CLSM을 결합한 미소 간극 내 유체의 단면 온도 분포 측정 기법)

  • Jeong, Dong-Woon;Lee, Sang-Yong
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.30 no.9 s.252
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    • pp.834-841
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    • 2006
  • In the present wort the Laser-induced Fluorescence (LIF) technique and Confocal Laser Scanning Microscopy (CLSM) have been combined to measure the temperature distribution across a micro-scale liquid layer as a direct and non-invasive method. Only the fluorescent light emitted from a very thin volume around a focal plane can be selectively detected, and it enables us to measure the liquid temperatures even at the close vicinity of the walls. As an experimental verification, a test section consists of two flat plates (for heating and cooling, respectively) separated by about 240 microns was made, and the methanol mixed with a temperature-sensitive dye, Rhodamine B, was filled in the gap between them. The measured temperature distribution across the gap showed good linearity, which is a typical characteristic of conduction heat transfer through a thin liquid layer. In result, the CLSM-LIF technique proposed in the present study was found to be a promising method to measure the local temperatures in the liquid flow field in microfluidic devices.

Measurement of OH radical spectrum in counterflow burner using degenerate four wave mixing (DFWM(degenerate four wave mixing)을 이용한 대향류버너 화염내의 OH 라디칼 스펙트럼 측정)

  • 이은성;한재원
    • Korean Journal of Optics and Photonics
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    • v.7 no.2
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    • pp.142-149
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    • 1996
  • In non-saturation region, we measured the degenerate four wave mixing spectra of $X^2\;{\Pi}(v=0){\to}A^2{\Sigma}^+(v'=0)$ transition for OH in counterflow burner, which exists transiently in combustion reaction. We used forward box type geometry for phase matching. Calculating the population of each rotational level from the line intensities of R$_1$band and comparing it with Boltzmann distributions, we could obtain the temperatures of the flame at several points. Corrected for the absorption of incident laser fields, the final temperatures coincided with those measured by coherent anti-Stokes Raman Scattering within error $\pm$60 K near 2000 K. We also measured the concentration distribution of OH radical and it was compared to that measured by laser induced fluorescence.

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Flow Visualization of Flowfield Structures around an Aerospike Nozzle using LIF and PSP

  • NIIMI Tomohide;MORI Hideo;TANIGUCHI Mashio
    • 한국가시화정보학회:학술대회논문집
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    • 2004.12a
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    • pp.75-80
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    • 2004
  • Aerospike nozzles have been expected to be used for an engine of a reusable space shuttle to respond to growing demand for rocket-launching and its cost reduction. In this study, the flow field structures in any cross sections around clustered linear aerospike nozzles are visualized and analyzed, using laser induced fluorescence (LIF) of nitrogen monoxide seeded in the carrier gas of nitrogen. Since flow field structures are affected mainly by pressure ratio, the clustered linear aerospike nozzle is set inside a vacuum chamber to carry out the experiments in the wide range of pressure ratios from 75 to 200. Flow fields are visualized in several cross-sections, demonstrating the complicated three-dimensional flow field structures. Pressure sensitive paint (PSP) of PtTFPP bound by poly- IBM -co-TFEM is also applied to measurement of the complicated pressure distribution on the spike surface, and to verification of contribution of a truncation plane to the thrust. Finally, to examine the effect of the sidewalls attached to the aerospike nozzle, the flow fields around the nozzle with the sidewalls are compared with those without sidewalls.

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Measurement of Fluorescence Signal Strength of NO Particle Using ICCD (ICCD를 이용한 NO입자의 형광신호강도 측정)

  • 전용우;박원주;이광식;이홍식
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.15 no.4
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    • pp.23-30
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    • 2001
  • In this paper, the discharge image of NO particles wire-cylinder type electrode in the discharge reactor where AC dielectric barrier type corona discharge occurred and horizontal and vertical signal intensity at each flourescence emission during discharge and the horizontal and vertical signal strength of NO particles at flourescence emission wavelength band[236[nm], 247[nm]], were measured were measured by ICCD Camera. In addition discharge images and signal intensities in accordance with discharge time were measured to figure out the discharge mechanism. It was found that the strongest horizontal and vertical signal intensity of NO particles were observed at 247[nm] band, but no big difference in the horizontal and vertical signal intensity in accordance with discharge time was seen. In particular, the phenomenon image occuring inside the discharge reactor and wavelength ware able to be carried based on the measured data.

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