• Bulletin of the Korean Chemical Society
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• 제28권12호
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• pp.2253-2260
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• 2007

A series of tetra donor substituted [2.2]paracyclophane-based two-photon absorption (TPA) fluorophores were synthesized in neutral and cationic forms. The imaging activity of overall set of fluorophores was studied by the two-photon induced fluorescence (TPIF) method in a range of solvents. We also measured a clear progression toward a longer photoluminescence lifetime with increasing solvent polarity (intrinsic photoluminescence lifetime, τi: ~2 ns in toluene → 12-16 ns in water). The paracyclophane fluorophores with this unique property can be utilized as an optical polarity probe for the biomolecular substrates. The combined measurement of the two-photon fluorescence microscopy (TPM) cell image and TPIF lifetime can give us a better understanding of the biological processes and local environments in the cells.

• Journal of Photoscience
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• 제9권2호
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• pp.78-81
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• 2002

Fluorescence rise and decay curves of carotenoids were measured in solutions and in pigment protein complexes with a femtosecond time-resolved fluorescence spectroscopy. For linear carotenoids, the S$_2$ lifetimes showed the maximum value around n = 9-10. The conjugation of a keto-carbonyl group shortened the S$_2$lifetime and prolonged the S$_1$lifetime. The excitation relaxation dynamics within carotenoids and the excitation energy transfer kinetics from carotenoids to chlorophylls are discussed as a function of molecular structure of carotenoids.

• Bulletin of the Korean Chemical Society
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• 제20권7호
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• pp.796-800
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• 1999

Three anthrylazacrown ethers in which the anthracene fluorophore π system is separated from the electron donor atoms by one methylene group were synthesized, and their photophysical study was accomplished. These fluorescent compounds showed a maximum fluorescence intensity at pH=5 in aqueous solutions and a decrease in fluorescence intensity upon binding of paramagnetic metal cations (Mn 2+ (d 5 ), Co 2+ (d 7 ), Cu 2+ (d 9 )). The decrease in fluorescence intensity may be attributed to the paramagnetic effect of metal cations to deactivate the excited state by the nonradiative quenching process. The benzylic nitrogen was found to play an important role in changing fluorescence intensity. From the observed linear Stern-Volmer plot and the fluorescence lifetime independence of the presence of metal ions, it was inferred that the chelation enhanced fluorescence quenching (CHEQ) mechanism in the system is a ground state static quenching process. Enhanced fluorescence was also observed when an excess Na + ion was added to the quenched aqueous solution, and it was attributed to cation displacement of a complexed fluorescence quencher.

• BMB Reports
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• 제35권4호
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• pp.389-394
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• 2002

$[Ru(bpy)_2(dppz)]^2+$ (bpy=2,2'-bipyfidine, dppz=dipyrido[3,2-a:2',3'-c]phenazine) (RuBD), a long-lifetime metal-ligand complex, displays favorable photophysical properties. These include long lifetime, polarized emission, but no significant fluorescence from the complex that is not bound to DNA. To show the usefulness of this luminophore (RuBD) for probing the bending and torsional dynamics of nucleic acids, its intensity and anisotropy decays when intercalated into supercoiled and relaxed pTZ18U plasmids were examined using frequency-domain fluorometry with a blue light-emitting diode (LED) as the modulated light source. The mean lifetimes for the supercoiled plasmids (< $\tau$ >=148 ns) were somewhat shorter than those for the relaxed plasmids (< $\tau$ >=160 ns). This suggests that the relaxed plasmids were shielded more efficiently from water. The anisotropy decay data also showed somewhat shorter slow rotational correlation times for supercoiled plasmids (288 ns) than for the relaxed plasmids (355 ns). The presence of two rotational correlation times suggests that RuBD reveals both the bending and torsional motions of the plasmids. These results indicate that RuBD can be useful for studying both the bending and torsional dynamics of mucleic acids.

• 한국수산과학회지
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• 제24권6호
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• pp.397-404
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• 1991

남조류와 crytomonads를 포함한 식물플랑크톤의 일차적 형광특성을 in vivo 상태에서 조사하였다. 형광과 exciatation 스펙트럼을 측정한 견과, 약 580-640 nm 영역에 걸친 형광 스펙트럼이, 조사된 모든 식물플랑크톤으로부터 얻어졌다. 관찰된 형광 스펙트럼의 $\lambda_{max}$ 값은, Synechofoccus sp.(SYN)을 제외하고 모두 유사한 것으로 나타났다 $(\pm3nm)$. 관찰된 형광 스펙트럼의 형광소(fluorophore)를 밝히기 위하여, 식물플랑크톤의 형광 스펙트럼의 세기와 모양, $\lambda_{max}$를 식물플랑크톤에 존재하는 유기화합물의 표준용액의 것과 비교하였다. 식물플랑크톤의 fluorescence $lifetime(\lambda)$과 fluorescence decay curve를 식물플랑크톤에 존재하는 유기화합물의 표준용액의 $\lambda$값과 비교하였다. 580 nm의 형광 파장을 사용하여 얻은 식물플랑크톤의 fluorescence decay는 menoex-ponential과 biexponential decay를 보였으며, 식물플랑크톤의 $\lambda(1.39-1.95ns)$ b-phycoerythrin의 표준용액의 $\lambda(3.23ns)$값보다 현저히 작게 나타났다. 이는 이미 알려진 광합성 능력을 가진 intact cell 내부에서 엽록소 a와 biliprotein간의 효율적인 에너지 전달 체계에 의한 $\lambda$값의 단축으로 여겨지며, 580nm의 형광특성이 biliprotein에 의한 것임을 보여준다.

• Bulletin of the Korean Chemical Society
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• 제10권2호
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• pp.142-147
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• 1989

Fluorescence enhancements of ethidium bromide (EB) by solution species of low molecular weights such as DNA base molecules and nucleosides in water are reported. The degree of enhancements was determined by intensity as well as lifetime measurements for EB fluorescence. Experiments including solvent effects on absorbance and fluorescence spectra of EB, effects of protonation on the EB absorbance spectrum, and determination of equilibrium constants for EB-DNA bases have been performed to help explain the fluorescence enhancement. The results suggest that the excited state stabilization in the hydrophobic environment, the loss of torsional/vibrational energy of amino groups, and the change in the electronic transition characteristics are all responsible for the fluorescence enhancement.

• Journal of Photoscience
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• 제5권3호
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• pp.105-109
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• 1998

By using the liquid -phase-deposition (LPD) process, which has a potetnial of preparing organic inorganic composite materials, samples doped with benzo[f]quinoline (BfQ)into silica thia films wre prepared. We observed the fluorescene and fluorescene excitation spectra of the samples, as well as the fluorescence lifetimes and time-resoluved fluorescence spectra. The comparison of thefluorescence spectra in pH-controlled buffer solutions yields the results that the dominant species of BfQ in the LPD silica films is a protonated one. The fluorescence band assigned to a hydrogen-bonded species was observed on the samples prepared from the dipping solutions of 3 and 2 M hexafluorosilicic acid. The band assignment was confirmed by the fluorescence lifetime measurement. The FT-IR M hexaflurosilicic acid. The band assignment was confirmed by the flurescence lifetime meausurement. The FT-IR data proved the existence of included water in silica films prepared from the LPD process. The appearance of the band corresponding to the hydrogen-bonded species within LPD silica phases was explained by the proesence of included water. Depending on the preparation conditions of LPD silica films, the band assigned to protonated species shows bad shifts in a wavenumber region between the peak of hydrogen-bonded and typical protonated species. This implies that there is some distribution of steric conformation of protonated species of BfQ interacting with adsorbing sites of LPD silica. The time -resolved fluorescence spectra suggest that some relaxation process is involved in the conformation of BfQ doped into the solid phase of LPD silica.

• Nuclear Engineering and Technology
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• 제33권2호
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• pp.166-174
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• 2001

An apparatus for detecting remote real-time uranium concentration using an optrode was developed. An optrode to detect uranium fluorescence as remote real-time control was designed. Fluorescence intensity at time 2ero was derived by the fluorescence signal processing and the algorithm to exclude the quenching effect of various quenchers and temperature fluctuations. This apparatus employing the above deriving method and the optrode has an error range within 6% in spite of serious fluorescence lifetime changes due to the quenching effect and temperature fluctuations. The detection limit is 0.06 ppm and the linearity is excellent between 0.06 ppm and 2 ppm on the aqueous uranium solution.

• 방사성폐기물학회지
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• 제18권4호
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• pp.497-508
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• 2020

In the geochemical field, the chemical speciation of hexavalent uranium (U(VI)) has been widely investigated by performing measurements to determine its luminescence properties, namely the excitation, emission, and lifetime. Of these properties, the excitation has been relatively overlooked in most time-resolved laser fluorescence spectroscopy (TRLFS) studies. In this study, TRLFS and continuous-wave excitation-emission matrix spectroscopy are adopted to characterize the excitation properties of U(VI) surface species that interact with amorphous silica. The luminescence spectra of U(VI) measured from a silica suspension and silica sediment showed very similar spectral shapes with similar lifetime values. In contrast, the excitation spectra of U(VI) measured from these samples were significantly different. The results show that distinctive excitation maxima appeared at approximately 220 and 280 nm for the silica suspension and silica sediment, respectively.

• 한국세라믹학회:학술대회논문집
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• 한국세라믹학회 2004년도 춘계총회 및 연구발표회 초록집
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• pp.35.3-35.3
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• 2004