• Title/Summary/Keyword: Fluorescence dye

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The Study on the Excitation Lasers for NO Planar Laser-Induced Fluorescence Imaging (NO PLIF용 excitation 레이저에 관한 연구)

  • Kang, Kyung-Tae
    • 한국연소학회:학술대회논문집
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    • 1997.06a
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    • pp.13-21
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    • 1997
  • Excitations of eight pumping transitions for nitric oxide fluorescence imaging are analyzed under equivalent experimental conditions to determine the detection. Frequency mixed dye laser pumping, 1st anti-Stokes $H_2$ Raman of KrF excimer laser pumping and ArF excimer laser pumping show good sensitivities.

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Fluorescence Micropatterning Based on the Polymeric Photobase Generator Containing Oxime-Urethane Groups by Dansylation

  • Choi, Won San;Kim, Hak Soo;Chae, Kyu Ho
    • Rapid Communication in Photoscience
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    • v.2 no.1
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    • pp.24-27
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    • 2013
  • A polymeric photobase generator containing oxime-urethane groups is applied to a fluorescence micropatterning material. Polymer bearing oxime-urethane groups was prepared by copolymerization of methyl methacrylate with methacryloyloxyethyl benzophenoneoxime urethane (MBU). The reaction of amino groups in the irradiated copolymer film with dansyl chloride (Dns-Cl) was monitored by using UV absorption, IR absorption and fluorescence spectroscopy. The fluorescence spectrum of the Dns-Cl-treated irradiated copolymer film shows a strong fluorescence with a fluorescence maximum wavelength at 510 nm. A blue fluorescent micropattern with a line width of $2{\sim}3{\mu}m$ was obtained. Treatment of the irradiated copolymer film with Dns-Cl and rhodamine B mixture led to the formation of green, red, and orange-colored fluorescence micropatterns. Thus, various colored micropatterns on a single polymer film can be obtained by selective excitation of each dye molecules.

Fabrication and Performance Investigation of Surface Temperature Sensor Using Fluorescent Nanoporous Thin Film II (형광 나노 포러스 박막을 이용한 표면 온도 센서의 제작 및 성능 연구 II)

  • Kim, Hyun Jung;Yoo, Jaisuk;Park, Jinil
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.25 no.12
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    • pp.674-678
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    • 2013
  • We present a non-invasive technique to the measure temperature distribution in nano-sized porous thin films by means of the two-color laser-induced fluorescence (2-LIF) of rhodamine B. The fluorescence induced by the green line of a mercury lamp with the makeup of optical filters was measured on two separate color bands. They can be selected for their strong difference in the temperature sensitivity of the fluorescence quantum yield. This technique allows for absolute temperature measurements by determining the relative intensities on two adequate spectral bands of the same dye. To measure temperature fields, Silica (SiO2) nanoporous structure with 1-um thickness was constructed on a cover glass, and fluorescent dye was absorbed into these porous thin films. The calibration curves of the fluorescence intensity versus temperature were measured in a temperature range of $10-60^{\circ}C$, and visualization and measurement of the temperature field were performed by taking the intensity distributions from the specimen for the temperature field.

Metachromasy of Metylene Blue and Thionine by Analytical Fluorescence Spectroscopy (형광분광분석법에 의한 Methylene Blue와 Thionine의 Metachromasy)

  • Lee, Hoo-Seol
    • Journal of the Korean Applied Science and Technology
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    • v.14 no.2
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    • pp.103-114
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    • 1997
  • Metachromatic properties of admixture of methylene blue(MB) and thionine in aqueous solution has been studied by fluorescence spectroscopy. In spite of nonfluorescence character has been MB itself, mixing MB to monomeric concentration of thionine, new coaggregation band has been formed in shorter wave length than fluorescence of thionine because of MB was redistributed to thionine aggregate. It suggested that coaggregate of MB and thionine were more tightly formed than the each dye aggregate.

Two-Dimensional Behavior and J-Aggregate Formation of Merocyanine Dye Monolayers in Mutual Mixing (상호혼합에 의한 메로시아닌 색소 단분자막의 2차원 거동 및 J-회합체 형성)

  • Sin, Hun-Gyu;Kwon, Young-Soo
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.51 no.3
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    • pp.105-110
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    • 2002
  • J-aggregates in the mutual mixing LB films of [6Me-DS]$_{1-x}$ [DO]$_{x}$,[DS]$_{1-x}$ [DO]$_{x}$ and [DSe]$_{1-x}$ [DO]$_{x}$ have been studied by optical absorption, fluorescence and surface pressure-area isotherms. In [6Me-DS]$_{1-x}$ [DO]$_{x}$ films, sharp J-band absorption and fluorescence of [6Me-DS] are linearly shifted to the longer wavelength for the replacement of [6Me-DS] by [DO]. According to the x, a smooth shift of the limited area has been cleared. In the [DS]-[DO] system, the J-band is enhanced at 1:1 composition and strong fluorescence is also observed. Also, the presence of phase separation was suggested in the [DSe]-[DO] system, because the absorption spectra were decomposed into [DSe] and [DO] spectra. On the other hand, in the pressure-area isothermal study, reduction in the molecular occupying area of monolayers has been clarified. This could be ascribed to the enhancement of molecular ordering in J-aggregates. These facts are also believed to reflect the most closely packed arrangement of chromophores in the merocyanine dye monolayers. Thus, it was confirmed that the interaction between mixed dye molecules and the CdC1$_2$+KHCO$_3$subphases affected the J-aggregates of the LB films. Also. it is thought that the J-aggregates are formed non-dimensionally in LB films, such as solution synthesized [DX:DO] assemblies on mixing.s on mixing.

Miniaturized Fluorometer Based on Total Internal Reflector and Condensing Mirror

  • Jang, Dae-Ho;Yoo, Jae-Chern
    • Journal of the Optical Society of Korea
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    • v.17 no.1
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    • pp.81-85
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    • 2013
  • A miniaturized fluorescence detection system based on total internal reflection (TIR) configuration, which is applicable to detecting the presence of biological materials labeled with fluorescence dye in micro total analysis systems (${\mu}TAS$), is proposed. In conventional fluorescence testing and analysis devices, interference between the excitation light beam and the emitted light from dyes is unavoidable. This paper presents a fluorescence detection system based on TIR configuration that allows the excitation light beam and the emitted light to be spatially perpendicular to each other so as to minimize the interference where fluorescence emission is detected at the orthogonal angle to the excitation beam. We achieved the limit of detection of about 5 nmol/L with a high linearity of 0.994 over a wide range of 6-FAM mol concentration, being comparable to that in earlier studies.

Analysis on Red-colored Dyeing by using Non-destructive UV-visible and Fluorescence Spectrophotometry (비파괴 자외-가시 및 형광 분광 분석법을 이용한 적색계 전통 염료 분석)

  • Yun, Eunyoung;Kim, Yuran
    • Conservation Science in Museum
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    • v.12
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    • pp.9-17
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    • 2011
  • This study examined non-destructive UV-Vis spectrophotometry as well as 3-D fluorescence spectrophotometry of textile that made use of red dye such as Sappan wood, madder, Safflower, Gromwell. The authors produced two textile specimen that were dyed by not only two kinds of textile (cotton and silk) but also three kinds of mordanting (no-mordanting, alumen and iron), and they investigated effects of each dye material upon investigation results. At analysis with UV-Vis spectrophotometry of dyed textile specimen, dyeing made by sappan wood, madder and gromwell had significant difference depending upon mardant regardless of kinds of textile, and safflower had no significant difference depending upon textile and mordant. At analysis with 3D-fluorescence spectrophotometry, specimen dyed with sappan wood had difference with mordants, and with madder, there were difference with textiles, and safflower had inherent fluorescence spectrum regardless of textiles and mordants, while gromwell had no fluorescence spectrum.

A COMPARISON OF FLUORESCENCE EFFECT OF VARIOUS LIGHT SOURCES IN EARLY ENAMEL CARIES (초기 우식 병소에서 광원에 따른 형광효과 비교)

  • Jeon, Jeong-Hoon;Lee, Nan-Young;Lee, Chang-Seop;Lee, Sang-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.32 no.4
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    • pp.611-619
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    • 2005
  • The aim of this study was to apply the quantitative light-induced fluorescence(QLF) and use of fluorescein-enhanced QLF method for quantitative assessment of early enamel demineralization in vitro, comparing effectiveness of light sources : argon laser, halogen lamp, light emitting diode (LED) and plasma arc lamp. Sixty extracted teeth were used, prepared by gentle pumicing and coating in an acid-resistant nail-varnish, except for an exposure to a demineralizing solution. Teeth were removed at regular intervals (24, 48 and 72hrs), air-dried and QLF image were taken. Mineral loss, as measured by difference of optical density, was recorded. For dyeenhanced QLF a 0.075% sodium fluorescein dye was applied after QLF examination and mineral loss was recorded. The following result were obtained : 1. Comparing with mean difference of optical density, plasma arc lamp was higher than other light sources in all group (p<0.05). 2. Comparing each group with mean difference of optical density, there was significant different using plasma are lamp and halogen lamp. 3. For use of dye-enhanced QLF, comparing with mean difference of optical density, plasma arc lamp were higher than other light sources in all group(p<0.05). 4. With this model dye-enhanced QLF compared with QLF in optical density, dye-enhanced QLF was higher than QLF in optical density. From the results presented in this paper, it was concluded that plasma light source was more effective compared with other light source for the detection and the quantification of early enamel caries.

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Synthesis and Photoluminescent Property of Diheteryl-substituted Triphenylamine Compound (Diheteryl-substituted triphenylamine 화합물의 합성과 형광 특성)

  • Kim, Byung-Soon;Kim, Sung-Hoon;Son, Young-A
    • Textile Coloration and Finishing
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    • v.19 no.6
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    • pp.35-38
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    • 2007
  • FTriphenylamine dye compound having diheteryl moiety was synthesized and its photoluminescent property was investigated. Organic luminescent materials have received great attentions due to potential application subjects onto full color image displays. In this context, the dye (III) for light emitting materials was synthesized using 2-(4-amino-2-hydroxyphenyl)benzoxazole (I) and 4,4'-diformyltriphenylamine (II). It is well known that the amino groups of compound (I) react with carbonyl groups, especially an aldehyde, to afford azomethine linkages. The dye shows bulish-green fluorescence property, which is anticipated for the light-emitting material for display devices. In this context, our aim is to synthesize diheteryl-substituted triphenylamine fluorescent dye as an emitting material. The spectroscopic characteristics and the fluorescent properties of this dye molecule were examined and determined.

Characteristics of Ar flashlamp and Fluorescence of BBQ (Ar 섬광관의 방전 특성과 BBQ의 형광 강도)

  • Lim, Chun-Woo;Park, Sung-Jin;Bang, Hyung-Bae;Oh, Chul-Han
    • Proceedings of the KIEE Conference
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    • 1992.07b
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    • pp.958-960
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    • 1992
  • The fluorescence characteristics of near UV dye, BBQ pumped by Ar flashlamp have been studied for various electrical input energies applied to the flashlamp and argon fill pressures in the flash tube. The peak wavelength of fluorescence is about 380nm when the fluorescence intensity is at the peak value. The rise time of the fluorescence pulse is about 7 $\mu$sec and its FWHM is about 55$\mu$sec.

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